Microplastics in soils: Production, behavior process, impact on soil organisms, and related toxicity mechanisms.

In recent years, microplastics (MPs) pollution has become a hot ecological issue of global concern and MP pollution in soil is becoming increasingly serious. Studies have shown that MPs have adverse effects on soil biology and ecological functions. Although MPs are evident in soils, identifying their source, abundance, and types is difficult because of the complexity and variability of soil components. In addition, the effects of MPs on soil physicochemical properties (PCP), including direct effects such as direct interaction with soil particles and indirect effects such as the impact on soil organisms, have not been reported in a differentiated manner. Furthermore, at present, the soil ecological effects of MPs are mostly based on biological toxicity reports of their exudate or size effects, whereas the impact of their surface-specific properties (such as environmentally persistent free radicals, surface functional groups, charge, and curvature) on soil ecological functions is not fully understood. Considering this, this paper reviews the latest research findings on the production and behavioral processes of MPs in soil, the effects on soil PCP, the impacts on different soil organisms, and the related toxic mechanisms. The above discussion will enhance further understanding of the behavioral characteristics and risks of MPs in soil ecosystems and provide some theoretical basis for further clarification of the molecular mechanisms of the effects of MPs on soil organisms.

Toxic effects of environmentally persistent free radicals (EPFRs) on the surface of tire wear particles on freshwater biofilms: The alleviating role after sewage-incubation-aging.

The aquatic ecological risks posed by the surface-active components of tire wear particles (TWPs) are not fully understood. This study aimed to determine the acute (24 h exposure) aquatic toxicity effects of TWPs on freshwater biofilms in terms of total organic carbon (TOC), chlorophyll-a (Chl-a) abundance, quantum yield (ФM), and adenosine triphosphate (ATP). Three types of TWP were tested: TWPs produced via the typical wear of tires and roads (i.e., rolling friction (R-TWPs) and sliding friction (S-TWPs)) and cryogenically milled tire treads (C-TWPs). The results showed that the surface structural properties of the three TWPs differed significantly in morphology, bare composition, functional groups, and surface-active components (environmental persistent free radicals). The exposure of biofilms to the TWPs increased TOC and ATP at low concentrations (1 mg L-1) but inhibited them at high concentrations (50 mg L-1). All TWP types inhibited biofilm photosynthesis (reduced Chl-a and ФM) and altered the community structure of algae to varying degrees; in addition, the toxicity mechanisms of the TWPs contributed to the accumulation of reactive oxygen species and cell membrane (or cell-wall) fragmentation, leading to lactate dehydrogenase release. S-TWPs were the most toxic because their surface carried the highest environmental persistent free radicals. R-TWPs were the second most toxic, which was attributed to their smaller particle size. The toxicity of all TWPs was tested after sewage incubation aging. The results showed that the toxicity of all TWPs reduced as the sewage covered their surface components and active sites. This process also reduced the differences in toxicity among the TWPs. This study filled a research gap in our understanding of aquatic toxicity caused by the surface structural properties of tire microplastics and has implications for the study of microplastic biotoxicity mechanisms.

Tire wear particles in different water environments: occurrence, behavior, and biological effects-a review and perspectives.

As an important source of microplastics, the water ecological risk of tire wear particles (TWPs) has attracted widespread attention worldwide. However, the occurrence and behavior of TWPs and their biological effects in water environments have not been clearly analyzed. For example, most contemporary studies have focused on the evaluation of the aquatic toxicity of TWPs leachate, and little attention has been paid to the behavior process and potential risks of its surface properties in water environments. In addition, most studies rely on preparing TWPs under laboratory conditions or purchasing commercial TWPs for studying their water environmental behavior or exposure. These obviously cannot meet the requirements of accurate assessment of water ecological risks of TWPs. As thus, in addition to describing the occurrence, distribution, and (aging) transformation of TWPs in different water environments, we further tried to explain the potential water environment behavior process and multiple pathways leading to potential adverse impacts of TWPs on aquatic organisms from the perspectives of particle self-toxicity and release toxicity, as well as synergistic effects of TWPs and other substances are also discussed. The existing data, such as studies on the self-characteristics of TWPs, environmental factors, and subjects, are insufficient to comprehensively evaluate the recent changes in essential water ecosystem services and multifunctions caused by TWPs, implying that the impact of TWPs on water environmental health needs to be further evaluated, and the corresponding countermeasures should be recommended. In this context, the current review provides an outlook on future research on TWPs in aquatic environments.

Effects of tire wear particles with and without photoaging on anaerobic biofilm sulfide production in sewers and related mechanisms.

Tire wear particles (TWPs) are considered to be one of the major sources of microplastics (MPs) in sewers; however, little has been reported on the surface properties and photochemical behavior of TWPs, especially in terms of their environmental persistent radicals, leachate type, and response after photoaging. It is also unknown how TWPs influence the production of common pollutants (e.g., sulfides) in anaerobic biofilms in sewers. In our study, the effects of cryogenically milled tire treads (C-TWPs) and their corresponding photoaging products (photoaging-TWPs, A-TWPs) on anaerobic biofilm sulfide production in sewers and related mechanisms were studied. The results showed that the two TWPs at a low concentration (0.1 mg L-1) exerted no significant (p > 0.05) effects on sulfide yield, whereas exposure to a high concentration of TWPs (100 mg L-1) inversely affected sulfide yield, with A-TWPs exerting a significant inhibitory effect on sulfide yield in the sewers (p < 0.01). The main reason was that A-TWPs carried higher concentrations of reactive environmental persistent radicals on their surfaces after photoaging than C-TWPs, which could induce the formation of oxygen radicals. In addition, A-TWPs were more uniformly distributed in the wastewater system and could penetrate the biofilm to damage bacterial cells, and their ability to leach polycyclic aromatic hydrocarbons and heavy metals such as zinc additives enhanced their toxic effects. In contrast, C-TWPs contributed significantly to sulfide production (p < 0.01), primarily because of their low biotoxicity, ability to leach a considerable amount of sulfide, and stimulatory effect on anaerobic biofilm surface sulfate-reducing bacteria. Our study complements the toxicity studies of the TWPs particles themselves and provides insight on a new influencing factor for determining the changes in sulfide generation and control measures in sewers.

A review on the generation, discharge, distribution, environmental behavior, and toxicity (especially to microbial aggregates) of nano-TiO2 in sewage and surface-water and related research prospects.

This article reviews the nano-effects and applications of different crystalline nano­titanium dioxide (nano-TiO2), identifies their discharge, distribution, behavior, and toxicity to aquatic organisms (focusing on microbial aggregates) in sewage and surface-water, summarizes related toxicity mechanisms, and critically proposes future perspectives. The results show that: 1) based on crystal type, application boundaries of nano-TiO2 have become clear, extending from traditional manufacturing to high-tech fields; 2) concentration of nano-TiO2 in water is as high as hundreds of thousands of µg/L (sewage) or several to dozens of µg/L (surface-water) due to direct application or indirect release; 3) water environmental behaviors of nano-TiO2 are mainly controlled by hydration conditions and particle characteristics; 4) aquatic toxicities of nano-TiO2 are closely related to their water environmental behavior, in which crystal type and tested species (such as single species and microbial aggregates) also play the key role. Going forward, the exploration of the toxicity mechanism will surely become a hot topic in the aquatic-toxicology of nano-TiO2, because most of the research so far has focused on the responses of biological indicators (such as metabolism and damage), while little is known about the stress imprint caused by the crystal structures of nano-TiO2 in water environments. Additionally, the aging of nano-TiO2 in a water environment should be heeded to because the continuously changing surface structure is bound to have a significant impact on its behavior and toxicity. Moreover, for microbial aggregates, comprehensive response analysis should be conducted in terms of the functional activity, surface features, composition structure, internal microenvironment, cellular and molecular level changes, etc., to find the key point of the interaction between nano-TiO2 and microbial aggregates, and to take mitigation or beneficial measures to deal with the aquatic-toxicity of nano-TiO2. In short, this article contributes by 1) reviewing the research status of nano-TiO2 in all aspects: application and discharge, distribution and behavior, and its aquatic toxicity; 2) suggesting the response mechanism of microbial aggregates and putting forward the toxigenic mechanism of nanomaterial structure; 3) pointing out the future research direction of nano-TiO2 in water environment.