Preoperative prediction of lymphovascular invasion in patients with T1 breast invasive ductal carcinoma based on radiomics nomogram using grayscale ultrasound.

Purpose: The aim of this study was to develop a radiomics nomogram based on grayscale ultrasound (US) for preoperatively predicting Lymphovascular invasion (LVI) in patients with pathologically confirmed T1 (pT1) breast invasive ductal carcinoma (IDC). Methods: One hundred and ninety-two patients with pT1 IDC between September 2020 and August 2022 were analyzed retrospectively. Study population was randomly divided in a 7: 3 ratio into a training dataset of 134 patients (37 patients with LVI-positive) and a validation dataset of 58 patients (19 patients with LVI-positive). Clinical information and conventional US (CUS) features (called clinic_CUS features) were recorded and evaluated to predict LVI. In the training dataset, independent predictors of clinic_CUS features were obtained by univariate and multivariate logistic regression analyses and incorporated into a clinic_CUS prediction model. In addition, radiomics features were extracted from the grayscale US images, and the radiomics score (Radscore) was constructed after radiomics feature selection. Subsequent multivariate logistic regression analysis was also performed for Radscore and the independent predictors of clinic_CUS features, and a radiomics nomogram was developed. The performance of the nomogram model was evaluated via its discrimination, calibration, and clinical usefulness. Results: The US reported axillary lymph node metastasis (LNM) (US_LNM) status and tumor margin were determined as independent risk factors, which were combined for the construction of clinic_CUS prediction model for LVI in pT1 IDC. Moreover, tumor margin, US_LNM status and Radscore were independent predictors, incorporated as the radiomics nomogram model, which achieved a superior discrimination to the clinic_CUS model in the training dataset (AUC: 0.849 vs. 0.747; P < 0.001) and validation dataset (AUC: 0.854 vs. 0.713; P = 0.001). Calibration curve for the radiomic nomogram showed good concordance between predicted and actual probability. Furthermore, decision curve analysis (DCA) confirmed that the radiomics nomogram had higher clinical net benefit than the clinic_CUS model. Conclusion: The US-based radiomics nomogram, incorporating tumor margin, US_LNM status and Radscore, showed a satisfactory preoperative prediction of LVI in pT1 IDC patients.

[Effect of IL-10 gene polymorphism on periodontal microecology in patients with periodontitis].

PURPOSE: To investigate the effect of IL-10 gene polymorphism on periodontal microecology in patients with periodontitis. METHODS: Seventy-nine patients with periodontitis (experimental group) and 50 healthy individuals (control group) visiting Zibo First Hospital from June 2018 to March 2019 were enrolled. The experimental group was further divided into two groups according to the severity of periodontitis, namely moderate periodontitis (n=47) and severe periodontitis (n=32). Real-time quantitative PCR was used to detect the level of bacterial colonization in the subgingival plaque of all subjects. The genotype and allele frequencies at -1082, -819, and -592 loci in IL-10 were detected by PCR. Multivariate analysis was used to explore the relationship between IL-10 genotypes and bacterial colonization level in subgingival plaque. The data were processed by SPSS 2.0 software package. RESULTS: Actinomycetes actinomycetes (A.a) colonization in patients with moderate periodontitis was significantly lower than that of patients with severe periodontitis (P<0.05). The amount of Porphyromonas gingivalis(P.g) and A.a in patients with moderate periodontitis and severe periodontitis was significantly higher than that in the control group (P<0.05). The proportion of CC genotypes in the -819 locus of patients with moderate periodontitis was significantly lower than that of the control group, and the proportion of TT genotype was significantly higher than that of the control group(P<0.05). The proportion of CC, TC and TT genotypes showed no significant difference between patients with moderate periodontitis and those with severe periodontitis (P>0.05). The proportion of CC genotypes in the -592 locus of patients with moderate periodontitis was significantly lower than that of the control group, and the proportion of AA genotype was significantly higher than that of the control group (P<0.05). In moderate periodontitis, A.a colonization amount at -592 gene locus of AA genotype was significantly higher than that of AC genotype(P<0.05). In moderate periodontitis, A.a colonization amount at of -819 gene locus of TT genotype was significantly higher than that of TC genotype (P<0.05). Multivariate analysis showed that IL-10-592-AA genotype and -819-TT genotype were closely related to A.a colonization in moderate periodontitis (P<0.05). CONCLUSIONS: IL-10 gene polymorphism is the influencing factor of subgingival microecological environment in patients with moderate periodontitis, among which IL-10-592- AA genotype and -819-TT genotype have the most obvious correlation with A.a colonization level.

Intracellular and current source density analysis of pretectal input to the optic tectum of the frog.

OBJECTIVE: Electrophysiological examination of the ipsilateral pretectotectal projection has proved that pretectal cells elicit strong suppressive responses to the ipsilateral tectum. However, the neural mechanisms underlying the contralateral pretectotectal prejection are still obscure. The present study aimed to examine the synaptic nature of pretectal nuclei and contralateral tectal cells, and to demonstrate the spatiotemporal pattern of neuronal activity in the 2 main brain structures. METHODS: Intracellular recording and current source density (CSD) analysis were used to test the complexity of neuronal mechanism of pretectotectal information transfer. RESULTS: The pretectal stimulation elicited only one type of response on the contralateral tectum, the inhibitory postsynaptic potential (IPSP). The majority of contra-induced IPSPs were assumed to be polysynaptically driven. In the CSD analysis, only one sink with short latency was observed in each profile. The ipsilateral projection produced a prominent monosynaptic sink in layer 8 of tectum. Recipient neurons were located in layers 6 and 7 of tectum. The result confirmed former findings from ipsilateral intracellular recordings. CONCLUSION: These results suggest the following neuronal circuit: afferents from the pretectal nuclei broadly inhibit both tectal neuron, and since no second sink occurs in tectal layers, the pretectotectal excitatory afferents probably do not extend over the whole tectum, but are within limited state. The results of intracellular recording and CSD analysis further provide evidence of how pretectal afferent activity flows within the tectal laminae.