Pharmacological inhibition of the LIF/LIFR autocrine loop reveals vulnerability of ovarian cancer cells to ferroptosis.

Of all gynecologic cancers, epithelial-ovarian cancer (OCa) stands out with the highest mortality rates. Despite all efforts, 90% of individuals who receive standard surgical and cytotoxic therapy experience disease recurrence. The precise mechanism by which leukemia inhibitory factor (LIF) and its receptor (LIFR) contribute to the progression of OCa remains unknown. Analysis of cancer databases revealed that elevated expression of LIF or LIFR was associated with poor progression-free survival of OCa patients and a predictor of poor response to chemotherapy. Using multiple primary and established OCa cell lines or tissues that represent five subtypes of epithelial-OCa, we demonstrated that LIF/LIFR autocrine signaling is active in OCa. Moreover, treatment with LIFR inhibitor, EC359 significantly reduced OCa cell viability and cell survival with an IC50 ranging from 5-50 nM. Furthermore, EC359 diminished the stemness of OCa cells. Mechanistic studies using RNA-seq and rescue experiments unveiled that EC359 primarily induced ferroptosis by suppressing the glutathione antioxidant defense system. Using multiple in vitro, ex vivo and in vivo models including cell-based xenografts, patient-derived explants, organoids, and xenograft tumors, we demonstrated that EC359 dramatically reduced the growth and progression of OCa. Additionally, EC359 therapy considerably improved tumor immunogenicity by robust CD45+ leukocyte tumor infiltration and polarizing tumor-associated macrophages (TAMs) toward M1 phenotype while showing no impact on normal T-, B-, and other immune cells. Collectively, our findings indicate that the LIF/LIFR autocrine loop plays an essential role in OCa progression and that EC359 could be a promising therapeutic agent for OCa.

Abnormal functional connectivity of white-matter networks and gray-white matter functional networks in patients with NMOSD.

Cognitive impairment (CI) has been reported in 29-70% of patients with neuromyelitis optica spectrum disorder (NMOSD). Abnormal white matter (WM) functional networks that correlate with cognitive functions have not been studied well in patients with NMOSD. The aim of the current study was to investigate functional connectivity (FC), spontaneous activity, and functional covariance connectivity (FCC) abnormalities of WM functional networks in patients with NMOSD and their correlation with cognitive performance. Twenty-four patients with NMOSD and 24 healthy controls (HCs) were included in the study. Participants underwent brain resting-state functional magnetic resonance imaging (fMRI) and the Montreal Cognitive Assessment (MoCA). Eight WM networks and nine gray matter (GM) networks were created. In patients, WM networks, including WM1-4, WM1-8, WM2-6, WM2-7, WM2-8, WM4-8, WM5-8 showed reduced FC (P < 0.05). All WM networks except WM1 showed decreased spontaneous activity (P < 0.05). The major GM networks demonstrated increased/decreased FC (P < 0.05), whereas GM7-WM7, GM8-WM4, GM8-WM6 and GM8-WM8 displayed decreased FC (P < 0.05). The MoCA results showed that two-thirds (16/24) of the patients had CI. FC and FCC in WM networks were correlated negatively with the MoCA scores (P < 0.05). WM functional networks are multi-layered. Abnormal FC of WM functional networks and GM functional networks may be responsible for CI.

An environmentally-friendly permeable liquid salt pyrolysis method based on capillary heat transfer for recycling waste insulator materials.

Molten salt pyrolysis technology stands out as a potent approach for achieving efficient degradation and energy recovery of composite organic materials. Nevertheless, challenges such as the high melting point of molten salt, product destruction, and the complexities of treating waste salt pose significant limitations to the widespread application and popularization of this technology. To tackle these issues, this study proposes a salt-assisted pyrolysis method based on capillary heat transfer called permeable liquid salt pyrolysis. Focusing on abandoned power industry insulators, the research delves into the thermal and mass transfer model of cluster-embedded materials under non-molten salt conditions. The investigation reveals that the capillary between glass fiber and resin proves beneficial in enhancing heat transfer conditions by creating a novel phase known as permeate liquid. Results demonstrate that salt-assisted pyrolysis can substantially lower the required temperature and enhance the pyrolysis reaction rate, achieving a maximum degradation efficiency of 98.99 %. Additionally, the pyrolysis products undergo in-situ modification, with a notable reduction in benzene series compounds ranging from 68 % to 85 %. Furthermore, an erosion diffusion capillary mode is established. This study presents an environmentally-friendly approach to recycle and modify products derived from waste resin-based composite materials generated in the electric power industry.

In situ high-valued transformation of nonmetals in waste printed circuit boards into supercapacitor electrodes with excellent performance.

Nonmetals in waste printed circuit boards after metal separation containing brominated resin and fiberglass are considered hazardous and low-recoveryvalue e-waste. However, if these nonmetals are not treated or are improperly treated, they can cause serious environmental pollution. Therefore, there is an urgent and significant need to develop an efficient recycling process for these nonmetals. Based on the concept of high-valued recycling of waste, this study in situ utilized such nonmetals to prepare a porous supercapacitor electrode through a facile carbonization, activation, and carbon thermal reduction process. The results indicated that the activation was a key role in constructing a porous structure. The optimal parameters for activation were a temperature of 800 °C, mass ratio of KOH to pyrolytic residues of 2, and an activation time of 1 h. The electrode materials exhibited a surface area of 589 m2 g-1 and hierarchical porous structures. In addition, the supercapacitors exhibited a capacitance of 77.14 mF cm-2 (62.5 mF cm-2) at 0.5 mA cm-2 (100 mV s-1). Moreover, the supercapacitors had excellent temperature resistance and adaptability. The capacitance retention was 89.36% and 90% at -50 °C and 100 °C after 10 000 cycles, respectively. This study provides a high-valued recycling strategy to utilize the nonmetals in e-waste as energy materials.

Single-cell transcriptomic analyses of tumor microenvironment and molecular reprograming landscape of metastatic laryngeal squamous cell carcinoma.

Laryngeal squamous cell carcinoma (LSCC) is a malignant tumor with a high probability of metastasis. The tumor microenvironment (TME) plays a critical role in cancer metastasis. To gain insights into the TME of LSCC, we conducted single-cell RNA-seq (scRNA-seq) on samples collected from LSCC patients with or without lymphatic metastasis. The stem and immune cell signatures in LSCC suggest their roles in tumor invasion and metastasis. Infiltration of a large number of regulatory T cells, dysplastic plasma cells, and macrophages that are at the early development stage in the cancerous tissue indicates an immunosuppressive state. Abundant neutrophils detected at the cancer margins reflect the inflammatory microenvironment. In addition to dynamic ligand-receptor interactions between the stromal and myeloid cells, the enhanced autophagy in endothelial cells and fibroblasts implies a role in nutrient supply. Taken together, the comprehensive atlas of LSCC obtained allowed us to identify a complex yet unique TME of LSCC, which may help identify potential diagnostic biomarkers and therapeutic targets for LSCC.

Pollution characteristics and release mechanism of microplastics in a typical end-of-life vehicle (ELV) recycling base, East China.

Microplastics (MPs) is a novel and significant pollution due to its eco-environmental hazards and ubiquity. In end-of-life vehicle (ELV) recycling base, MPs are widely distributed but have rare reported in scientific literature. In this study, a comprehensive analysis of MPs was conducted in a typical ELV recycling base. MPs were found in all samples at different sampling sites and environmental mediums. A total of 34 polymer types were detected by µ-FTIR, and the main polymers include PE-PP, ABS, polyester resin, nylon, and PEU plastic. MPs were released from the crushing, tearing, and breaking of plastic parts in ELVs. They were in high content in ground dust, with the abundance of 737-29,021 p/5 g D (the average abundance of 5552 ± 6435 p/5 g D). The abundance, shape, color, and size of MPs are related with functional areas of ELV recycling. Heavy metals could be adsorbed on MPs, and their contents on MPs have a significant correlation with those in the corresponding dust samples. At last, some specific MPs control measures, such as changing transportation mode, using dust-proof cloths, and equipping dust removal equipment, have been put forward.

Intelligent leaching rare earth elements from waste fluorescent lamps.

Rare earth elements (REEs), one of the global key strategic resources, are widely applied in electronic information and national defense, etc. The sharply increasing demand for REEs leads to their overexploitation and environmental pollution. Recycling REEs from their second resources such as waste fluorescent lamps (WFLs) is a win-win strategy for REEs resource utilization and environmental production. Pyrometallurgy pretreatment combined with acid leaching is proven as an efficient approach to recycling REEs from WFLs. Unfortunately, due to the uncontrollable components of wastes, many trials were required to obtain the optimal parameters, leading to a high cost of recovery and new environmental risks. This study applied machine learning (ML) to build models for assisting the leaching of six REEs (Tb, Y, Eu, La, and Gd) from WFLs, only needing the measurement of particle size and composition of the waste feed. The feature importance analysis of 40 input features demonstrated that the particle size, Mg, Al, Fe, Sr, Ca, Ba, and Sb content in the waste feed, the pyrometallurgical and leaching parameters have important effects on REEs leaching. Furthermore, their influence rules on different REEs leaching were revealed. Finally, some verification experiments were also conducted to demonstrate the reliability and practicality of the model. This study can quickly get the optimal parameters and leaching efficiency for REEs without extensive optimization experiments, which significantly reduces the recovery cost and environmental risks. Our work carves a path for the intelligent recycling of strategic REEs from waste.

A green strategy for upcycling utilization of core parts from end-of-life vehicles (ELVs): Pollution source analysis, technology flowchart, technology upgrade.

The disposal of end-of-life vehicles (ELVs) is an issue of great concern to the society, because of its huge amount, resource value and environmental pollution. A wide variety of pollutants generate and release during the recycling process. However, previous studies are piecemeal and segmentary, the correlation between treatment flowchart and pollution is unknown, and pollution source analysis in ELV recycling and core parts (engine, gear box, etc.) remanufacturing bases is still a challenge. In this study, the aim is to propose a green strategy for upcycling utilization of ELV part based on pollution source analysis, technology flowchart, and technology upgrade. We synthetically analyzed current typical ELV dismantling and core part remanufacturing processes of ELVs. A total of 36 volatile organic compound (VOC) species and 7 heavy metals were found in dismantling process, and 61 VOC species were detected in remanufacturing process. Based on statistical analysis and treatment process characteristics, 18 pollution fingerprints were constructed. At last, an intelligent dismantling and upcycle utilization line for ELVs has been developed to improve production efficiency and reduce pollution release.

Discovery of fast and stable proton storage in bulk hexagonal molybdenum oxide.

Ionic and electronic transport in electrodes is crucial for electrochemical energy storage technology. To optimize the transport pathway of ions and electrons, electrode materials are minimized to nanometer-sized dimensions, leading to problems of volumetric performance, stability, cost, and pollution. Here we find that a bulk hexagonal molybdenum oxide with unconventional ion channels can store large amounts of protons at a high rate even if its particle size is tens of micrometers. The diffusion-free proton transport kinetics based on hydrogen bonding topochemistry is demonstrated in hexagonal molybdenum oxide whose proton conductivity is several orders of magnitude higher than traditional orthorhombic molybdenum oxide. In situ X-ray diffraction and theoretical calculation reveal that the structural self-optimization in the first discharge effectively promotes the reversible intercalation/de-intercalation of subsequent protons. The open crystal structure, suitable proton channels, and negligible volume strain enable rapid and stable proton transport and storage, resulting in extremely high volumetric capacitance (~1750 F cm-3), excellent rate performance, and ultralong cycle life (>10,000 cycles). The discovery of unconventional materials and mechanisms that enable proton storage of micrometer-sized particles in seconds boosts the development of fast-charging energy storage systems and high-power practical applications.

Enhancing Debromination Efficiency through Introducing Water Vapor Atmosphere to Overcome Limitations of Conventional Pyrolysis.

Bromine removal is significant in the recycling of waste printed circuit boards (WPCBs). This study found that the critical factors limiting the debromination efficiency of conventional pyrolysis are the formation of coke impeding mass transfer and conversion of bromine into less volatile species, such as coking-Br and copper bromide. According to frontier molecular orbital analysis and thermodynamic equilibrium analysis, C-O bonds of resin are sites prone to electrophilic reactions and copper bromide in residue may undergo hydrolysis; therefore, introducing H2O during pyrolysis was a feasible method for thorough debromination. Through pyrolysis in a water vapor atmosphere, the diffusion limitation of debromination was overcome, and resin was converted into light components; thereby, rapid and deep removal of bromine was achieved. The result indicated that 99.7% of bromine was removed, and the residue could be used as a clean secondary resource. According to life-cycle assessment, pyrolysis of WPCBs in water vapor could be expected to reduce 77 Kt of CO2 emission and increase financial benefits by 60 million dollars, annually.

Research on compound pollution characteristics and health risk evaluation of particulate matter and heavy metals in waste glass recycling process.

Owing to the large amount of waste glass generated, the waste glass recycling base is an indispensable municipal supporting facility of a sustainable city. However, waste glass recycling is a complex process involving stages such as multiple-stage crushing and material sorting. Consequently, waste glass recycling base has a considerable impact on the surrounding environment, such as health risk of particulate matter on workers. In this study, we aimed to perform a comprehensive investigation and analysis of compound pollution characteristics and health risk evaluation of particulate matter and heavy metals generated from waste glass recycling process. Soil, particulate fallout, and glass samples were collected from inside and outside a recycling plant in eastern China. Our findings showed that the waste glass treatment process produces a large amount of air particulate matter, and the PM2.5 and PM10 concentrations can reach 3725 and 4055 µg/m3, respectively, in the production workshop during working hours. Meanwhile, the monitoring results show that the concentration of heavy metals on fine particles is higher compared to coarse particles. The high Zn and Pb concentrations detected in the soil and dustfall were proved to be derived from the glass raw materials. However, health risk assessment and particle deposition modeling in the human respiratory system revealed that heavy metals from the air particulate matter have no significant carcinogenicity or non-carcinogenic risk. The Gaussian dispersion model showed that the impact of particulate matter on the surrounding environment and health of the surrounding residents is minimal. Furthermore, transportation is the major emission link according to the particulate emission calculation, indicating that it is imperative to upgrade and reform the existing processes of waste glass recycling. Taken together, this study provides a scientific basis for the green development of waste glass recycling process and further environmental information regarding waste glass recycling plants.

"Mn-locking" effect by anionic coordination manipulation stabilizing Mn-rich phosphate cathodes.

High-voltage cathodes with high power and stable cyclability are needed for high-performance sodium-ion batteries. However, the low kinetics and inferior capacity retention from structural instability impede the development of Mn-rich phosphate cathodes. Here, we propose light-weight fluorine (F) doping strategy to decrease the energy gap to 0.22 eV from 1.52 eV and trigger a "Mn-locking" effect-to strengthen the adjacent chemical bonding around Mn as confirmed by density functional theory calculations, which ensure the optimized Mn ligand framework, suppressed Mn dissolution, improved structural stability and enhanced electronic conductivity. The combination of in situ and ex situ techniques determine that the F dopant has no influence on the Na+ storage mechanisms. As a result, an outstanding rate performance up to 40C and an improved cycling stability (1000 cycles at 20C) are achieved. This work presents an effective and widely available light-weight anion doping strategy for high-performance polyanionic cathodes.

The Foreseeable Future of Spent Lithium-Ion Batteries: Advanced Upcycling for Toxic Electrolyte, Cathode, and Anode from Environmental and Technological Perspectives.

With the rise of the new energy vehicle industry represented by Tesla and BYD, the need for lithium-ion batteries (LIBs) grows rapidly. However, owing to the limited service life of LIBs, the large-scale retirement tide of LIBs has come. The recycling of spent LIBs has become an inevitable trend of resource recovery, environmental protection, and social demand. The low added value recovery of previous LIBs mostly used traditional metal extraction, which caused environmental damage and had high cost. Beyond metal extraction, the upcycling of spent LIBs came into being. In this work, we have outlined and particularly focus on sustainable upcycling technologies of toxic electrolyte, cathode, and anode from spent LIBs. For electrolyte, whether electrolyte extraction or decomposition, restoring the original electrolyte components or decomposing them into low-carbon energy conversion is the goal of electrolyte upcycling. Direct regeneration and preparation of advanced materials are the best strategies for cathodic upcycling with the advantages of cost and energy consumption, but challenges remain in industrial practice. The regeneration of advanced graphite-based materials and battery-grade graphite shows us the prospect of regeneration of anode. Furthermore, the challenges and future development of spent LIBs upcycling are summarized and discussed from technological and environmental perspectives.

Recycling Hazardous and Valuable Electrolyte in Spent Lithium-Ion Batteries: Urgency, Progress, Challenge, and Viable Approach.

Recycling spent lithium-ion batteries (LIBs) is becoming a hot global issue due to the huge amount of scrap, hazardous, and valuable materials associated with end-of-life LIBs. The electrolyte, accounting for 10-15 wt % of spent LIBs, is the most hazardous substance involved in recycling spent LIBs. Meanwhile, the valuable components, especially Li-based salts, make recycling economically beneficial. However, studies of electrolyte recycling still account for only a small fraction of the number of spent LIB recycling papers. On the other hand, many more studies about electrolyte recycling have been published in Chinese but are not well-known worldwide due to the limitations of language. To build a bridge between Chinese and Western academic achievements on electrolyte treatments, this Review first illustrates the urgency and importance of electrolyte recycling and analyzes the reason for its neglect. Then, we introduce the principles and processes of the electrolyte collection methods including mechanical processing, distillation and freezing, solvent extraction, and supercritical carbon dioxide. We also discuss electrolyte separation and regeneration with an emphasis on methods for recovering lithium salts. We discuss the advantages, disadvantages, and challenges of recycling processes. Moreover, we propose five viable approaches for industrialized applications to efficiently recycle electrolytes that combine different processing steps, ranging from mechanical processing with heat distillation to mechanochemistry and in situ catalysis, and to discharging and supercritical carbon dioxide extraction. We conclude with a discussion of future directions for electrolyte recycling. This Review will contribute to electrolyte recycling more efficiently, environmentally friendly, and economically.

Correlated factors for Li-ion migration in ionic conductors with the fcc anion sublattice.

The development of solid-state electrolytes (SSEs) with high lithium ionic conductivities is critical for the realization of all-solid-state Li-ion batteries. Crystal structure distortions, Li polyhedron volumes, and anion charges in SSEs are reported to affect the energy landscapes, and it is paramount to investigate their correlations. Our works uncover the cooperative effect of lithium site distortions, anion charges, and lattice volumes on Li-ion migration energy barrier in superionic conductors of LiMS2 (M = Sc, Ti, V, Cr, Mn, Fe, Co, and Ni) and Li2MO3 (M = Sc, Ti, V, Cr, Mn, Fe, Co, and Ni). Combined with the Least Absolute Shrinkage and Selection Operator analyses, the volume and Continuous symmetrical methods (CSMs) of Li tetrahedral (Tet) sites appear to have a larger effect on the manipulation of Ea for Li migration, compared to that of Li octahedral (Oct) sites, which is further confirmed by the results from the face-centered cubic (fcc) anion lattice model. For the Tet-Oct-Tet Li migration path, the CSM (the volume of Li site) has a negative (positive) correlation with Ea, while for the Oct-Tet-Oct Li migration paths, opposite correlations have been observed. The understanding of the correlation between site preference, anion charge, lattice volume, and structural distortion as well as the prediction model of Ea in terms of these three factors, namely, C-V-D model, could be useful for the design of solid-state electrolytes with lower activation energy.

Monitoring the Resources and Environmental Impacts from the Precise Disassembly of E-Waste in China.

Due to the dispersed distribution of e-waste and crude disassembly in traditional recycling, valuable metals are not traceable during their life cycle. Meanwhile, incomplete separation between metals and nonmetals reduces the economic value of disassembled parts, which leads to higher environmental costs for metal refining. Therefore, this study proposes a precise disassembly of e-waste to finely classify and recover metals in an environmentally friendly way. First, the macroscopic material flow of e-waste in China (source, flow, scrap, and recycling gap) was measured based on data collected by the government and 109 formal recycling enterprises. The sustainable recycling balance time points for e-waste recycling and scrap volumes were forecast by introducing an additional recycling efficiency. By 2030, the total scrap volume of e-waste is predicted to reach 133.06 million units. For precise disassembly, the main metals and their percentages from these typical e-wastes were measured based on material flow analysis combined with experimental methods. After precise disassembly, the proportion of reusable metals increases significantly. The CO2 emission of precise disassembly with the smelting process was the lowest compared with crude disassembly with smelting and ore metallurgy. The greenhouse gas for secondary metals Fe, Cu, and Al was 830.32, 1151.62, and 716.6 kg CO2/t metal, respectively. The precise disassembly of e-waste is meaningful for building a future resource sustainable society and for carbon emission reduction.

A comprehensive analysis on the source-exposure and diffusion rule of VOCs for end-life vehicles dismantling activities.

In recent years, the rapid development of technology has posed significant challenges to the waste management practices of the retired vehicle industry. How to minimize the environmental impact during the recycle process of scrap vehicle has emerged as a prevalent and pressing concern. This study employed statistical analysis and positive matrix factorization (PMF) model to assess the origin of Volatile Organic Compounds (VOCs) at a scrap vehicle dismantling location situated in China. The quantification of potential hazards to human health arising from identified sources was achieved through the integration of source characteristics with exposure risk assessment. In addition, fluent simulation was employed to examine the spatiotemporal dispersion of the pollutant concentration field and velocity profile. The findings of the study revealed that the activities of parts cutting, disassembling air conditioning and refined dismantling were responsible for 89.98 %, 84.36 %, and 78.63 % of the total air pollution accumulation, respectively. Additionally, it should be noted that the aforementioned sources accounted for 59.40 %, 18.44 %, and 4.86 % of the aggregate non-cancer risk. The cumulative cancer risk was determined to be the disassembling air conditioning process, with a contribution of 82.71 %. Meanwhile, the average concentration of VOC in soil around the disassembling air conditioning area is 8.4 times higher than the background value. The simulation revealed that pollutants were primarily dispersed within the factory at a height ranging from 0.75 m to 2 m, which corresponds to the human respiratory zone and the concentration of pollutants in the vehicle cutting area was observed to exceed normal levels by over 10 times. These findings of this study may serve as a foundation for improving of environmental protection measures of industrialization.

Intrinsic B cell TLR-BCR linked coengagement induces class-switched, hypermutated, neutralizing antibody responses in absence of T cells.

Maturation of antibody responses entails somatic hypermutation (SHM), class-switch DNA recombination (CSR), plasma cell differentiation, and generation of memory B cells, and it is thought to require T cell help. We showed that B cell Toll-like receptor 4 (TLR4)-B cell receptor (BCR) (receptor for antigen) coengagement by 4-hydroxy-3-nitrophenyl acetyl (NP)-lipopolysaccharide (LPS) (Escherichia coli lipid A polysaccharide O-antigen) or TLR5-BCR coengagement by Salmonella flagellin induces mature antibody responses to NP and flagellin in Tcrß-/-Tcrδ-/- and NSG/B mice. TLR-BCR coengagement required linkage of TLR and BCR ligands, "linked coengagement." This induced B cell CSR/SHM, germinal center-like differentiation, clonal expansion, intraconal diversification, plasma cell differentiation, and an anamnestic antibody response. In Tcrß-/-Tcrδ-/- mice, linked coengagement of TLR4-BCR by LPS or TLR5-BCR by flagellin induced protective antibodies against E. coli or Salmonella Typhimurium. Our findings unveiled a critical role of B cell TLRs in inducing neutralizing antibody responses, including those to microbial pathogens, without T cell help.

A method for using the residual energy in waste Li-ion batteries by regulating potential with the aid of overvoltage response.

The value of considerable residual energy in waste Li-ion batteries (WLIBs) is always neglected. At present, "this energy" is always wasted during the discharge process of WLIBs. However, if this energy could be reused, it would not only save a lot of energy but also avoid the discharge step of recycling of WLIBs. Unfortunately, the instability of WLIBs potential is a challenge to efficient utilization of this residual energy. Here, we propose a method that could regulate the cathode potential and current of the battery by simply adjusting the solution pH to utilize 35.08%, 88.4%, and 84.7% of the residual energy for removing heavy metal ions from wastewater, removing Cr (VI) from wastewater, and recovering copper from the solution, respectively. By taking advantage of the high internal resistance R of WLIBs and the sudden change of battery current I caused by iron passivation on the positive electrode of the battery, this method could induce the response of overvoltage η (η = IR) inside the battery at different pH levels to regulate the cathode potential µ of the battery to the three intervals. The potential ranges of the battery cathode corresponding to pH < 3.4, pH ≈ 3.4, and pH > 4 were µ > -0.47V, -0.47V < µ < -0.82V, and µ < -0.82V, respectively. This study provides a promising way and theoretical basis for the development of technologies for reusing residual energy in WLIBs.