Efficient adsorption of rhodamine B using synthesized Mg-Al hydrotalcite/ sodium carboxymethylcellulose/ sodium alginate hydrogel spheres: Performance and mechanistic analysis.

In this study, the sodium dodecyl sulfate intercalated modified magnesium-aluminum hydrotalcite/sodium alginate/sodium carboxymethylcellulose (modified LDHs/SA/CMC) composite gel spheres were synthesized and their efficacies in adsorbing the cationic dye rhodamine B (RhB) from aqueous solutions were evaluated. The effects of adsorption time, pH and temperature on the adsorption of RhB by spheres were investigated. Remarkably, the modified LDHs/SA/CMC gel spheres achieved adsorption equilibrium after 600 min at 25 °C, and the removal rate of RhB at 60 mg/L reached 91.49 % with the maximum adsorption capacity of 59.64 mg/g. The gel spheres maintained over 80 % efficacy across four adsorption cycles. Kinetic and isotherm analyses revealed that the adsorption of RhB conformed to the secondary kinetic model and the Langmuir isotherm, indicating a spontaneous and exothermic nature of the adsorption process. The adsorption mechanisms of modified LDHs/SA/CMC gel spheres on RhB dyes include electrostatic adsorption, hydrogen bonding and hydrophobic interactions. In conclusion, modified LDHs/SA/CMC gel sphere is a green, simple, recyclable and efficient adsorbent, which is expected to be widely used for the treatment of cationic dye wastewater.

Arbuscular mycorrhizal fungi enhanced the drinking water treatment residue-based vertical flow constructed wetlands on the purification of arsenic-containing wastewater.

Arsenic (As) is a toxic metalloid that poses a potential risk to the environment and human health. In this study, drinking water treatment residue (DWTR) and ceramsite-based vertical flow constructed wetlands (VFCWs) were built to purify As-containing wastewater. As a method of bioaugmentation, arbuscular mycorrhizal fungi (AMF) was inoculated to Pteris vittata roots to enhance the As removal of the VFCWs. The results showed that the As removal rates reached 87.82-94.29% (DWTR) and 33.28-58.66% (ceramsite). DWTR and P. vittata contributed 64.33-72.07% and 7.57-29% to the removal of As, while AMF inoculation intensified the As accumulation effect of P. vittata. Proteobacteria, the main As3+ oxidizing bacteria in the aquatic systems, dominated the microbial community, occupying 72.41 ± 7.76%. AMF inoculation increased As-related functional genes abundance in DWTR-based wetlands and provided a reliable means of arsenic resistance in wetlands. These findings indicated that the DWTR-based VFCWs with AMF inoculated P. vittata had a great purification effect on As-containing wastewater, providing a theoretical basis for the application of DWTR and AMF for As removal in constructed wetlands.

Removal of sulfamethoxazole and Cu, Cd compound pollution by arbuscular mycorrhizal fungi enhanced vertical flow constructed wetlands.

The combined pollution of antibiotics and heavy metals (HMs) has a serious impact on the water ecological environment. Previous researches mainly focused on the removal of antibiotics or HMs as single pollutants, with limited investigation into the treatment efficiencies and underlying mechanisms associated with their co-occurring pollution. In this study, 16 micro vertical flow constructed wetlands (MVFCWs) were constructed to treat composite wastewater consisting of sulfamethoxazole (SMX), copper (Cu) and cadmium (Cd), involving two different inoculation treatments (arbuscular mycorrhizal fungi (AMF) inoculated and uninoculated) and eight kinds of pollutant exposure (Control Check (CK), SMX, Cu, Cd, SMX + Cu, SMX + Cd, Cu + Cd, SMX + Cu + Cd). The findings of this study demonstrated that the inoculation of AMF in MVFCWs resulted in removal efficiencies of SMX, Cu, and Cd ranging from 18.70% to 80.52%, 75.18% to 96.61%, and 40.50% to 89.23%, respectively. Cu and CuCd promoted the degradation of SMX in the early stage and inhibited the degradation of SMX in the later stage. Cd did not demonstrate a comparable promotive impact on SMX degradation, and its addition hindered Cu removal. However, comparatively, the presence of Cu exerted a more pronounced inhibitory effect on Cd removal. Furthermore, the addition of Cu augmented the abundances of Proteobacteria, Bacteroidetes (at the phylum level) and Rhodobacter, Lacunisphaera and Flavobacterium (at the genus level), and Cu exposure showed a substantially stronger influence on the microbial community than that of Cd and SMX. AMF might confer protection to plants against HMs and antibiotics by enriching Nakamurella and Lacunisphaera. These findings proved that AMF-C. indica MVFCW was a promising system, and the inoculation of AMF effectively enhanced the simultaneous removal of compound pollution.

Performances and mechanisms of simultaneous removal of nitrate and phosphate by biofilter assembled with sponge iron/copper and corn cobs.

Sponge iron (SI) is a potential material for removing nitrate and phosphate from water. We decorated the SI with copper (Cu) to enhance its removal performance. To gain insight into the nitrate and phosphate removal utilizing SI/Cu and microbial coupling systems, three biofilters filled with corn cob (CC), corn cob + sponge iron (CS) and corn cob + sponge iron/copper (CSCu) were constructed. The results showed that the effluent NO3--N and PO43--P concentrations of CSCu remained consistently below 1 and 0.1 mg/L. The introduction of SI/Cu led to the enrichment of the Dechloromonas genus, making it the dominant microbial group, occupying 42.65% of the effective sequences. Modification of SI with Cu increased nitrogen cycle-related functional genes abundance in CSCu, with a 634% increase in nirS compared to CS. These findings proved that SI/Cu was a promising material, providing an approach to concomitantly removing nitrate and phosphate.

Plant and microbial communities responded to copper and/or tetracyclines in mycorrhizal enhanced vertical flow constructed wetlands microcosms with Canna indica L.

Arbuscular mycorrhizal fungi (AMF) play a significant role in pollutants removal in constructed wetlands (CWs). However, the purification effects of AMF on combined copper (Cu) and tetracycline (TC) pollution in CWs remains unknown. This study investigated the growth, physiological characteristics and AMF colonization of Canna indica L. living in vertical flow CWs (VFCWs) treated for Cu and/or TC pollution, the purification effects of AMF enhanced VFCWs on Cu and TC, and the microbial community structures. The results showed that (1) Cu and TC inhibited plant growth and decreased AMF colonization; (2) the removal rates of TC and Cu by VFCWs were 99.13-99.80% and 93.17-99.64%, respectively; (3) the growth, Cu and TC uptakes of C. indica and Cu removal rates were enhanced by AMF inoculation; (4) TC and Cu stresses reduced and AMF inoculation increased bacterial operational taxonomic units (OTUs) in the VFCWs, Proteobacteria, Bacteroidetes, Firmicutes and Acidobacteria were the dominant bacteria, and AMF inoculation decreased the relative abundance of Novosphingobium and Cupriavidus. Therefore, AMF could enhance the pollutants purification in VFCWs by promoting plant growth and altering the microbial community structures.

The function and community structure of arbuscular mycorrhizal fungi in ecological floating beds used for remediation of Pb contaminated wastewater.

Arbuscular mycorrhizal fungi (AMF) have been demonstrated to be ubiquitous in aquatic ecosystems. However, their distributions and ecological functions are rarely studied. To date, a few studies have combined sewage treatment facilities with AMF to improve removal efficiency, but appropriate and highly tolerant AMF strains have not been explored, and the purification mechanisms remain unclear. In this study, three ecological floating-bed (EFB) installations inoculated with different AMF inocula (mine AMF inoculum, commercial AMF inoculum and non-AMF inoculated) were constructed to investigate their removal efficiency for Pb-contaminated wastewater. The AMF community structure shifts in the roots of Canna indica inhabiting EFBs during the three phases (pot culture phase, hydroponic phase and hydroponic phase with Pb stress) were tracked utilizing quantitative real-time polymerase chain reaction and Illumina sequencing techniques. Furthermore, transmission electron microscopy (TEM) and energy-dispersive X-ray spectroscopy (EDS) were used to detect the Pb location in mycorrhizal structures. The results showed that AMF could promote host plant growth and enhance the Pb removal efficiency of the EFBs. The higher the AMF abundance, the better the effect of the AMF on Pb purification by EFBs. Both flooding and Pb stress decreased the AMF diversity but did not significantly inhibit the abundance. The three inoculation treatments showed different community compositions with different dominant AMF taxa in different phases, and an uncultured Paraglomus species (Paraglomus sp. LC516188.1) was found to be the most dominant (99.65 %) AMF in the hydroponic phase with Pb stress. The TEM and EDS analysis results showed that the Paraglomus sp. could accumulate Pb in plant roots through their fungal structures (intercellular mycelium, intracellular mycelium, etc.), which alleviated the toxic effect of Pb on plant cells and limited Pb translocation. The new findings provide a theoretical basis for the application of AMF in plant-based bioremediation of wastewater and polluted waterbodies.

The responses and detoxification mechanisms of dark septate endophytes (DSE), Exophiala salmonis, to CuO nanoparticles.

To understand the tolerance mechanisms of dark septate endophytes (DSE), Exophiala salmonis, to CuO nanoparticles (CuO-NPs) with different sizes (40 and 150 nm), we investigated the morphology, antioxidant response, Cu subcellular distribution, and the melanin gene expression in the mycelia of E. salmonis. E. salmonis was cultured in liquid and solid media under the stress of increasing CuO-NP concentrations (0, 50, 100, 150, and 250 mg/L). Results showed that (1) E. salmonis showed good CuO-NP tolerance, and the tolerance to CuO-NPs at 150 nm was stronger than that at 40 nm. A large number of agglomeration structures were observed on the mycelia surface with the exception of 50 mg/L CuO-NPs with a diameter of 150 nm. (2) CuO-NP stress significantly stimulated the production of antioxidant enzymes, particularly the CuO-NPs with small particle size (40 nm). (3) Cu uptaken by E. salmonis increased proportionally with the increase of CuO-NP concentration in the medium. More than 80% Cu was absorbed in cell wall of mycelia treated with a small particle size (40 nm). (4) FTIR analysis revealed that hydroxyl, amine, carboxyl, and phosphate groups were associated with CuO-NP binding regardless of particle size. (5) Fungal melanin content increased with the addition of CuO-NPs; the increase of melanin induced by CuO-NPs with small particle size (40 nm) was more significant. (6) The expression of 1,3,6,8-tetrahydroxynaphthalene reductase (Arp2) in the melanin synthesis pathway increased under the stress of CuO-NPs, and CuO-NPs with a small particle size (40 nm) caused a significant change in the expression level of Arp2 gene than those with a large particle size (150 nm). In conclusion, E. salmonis had a strong tolerance to CuO-NPs and mitigated the toxic effects of CuO-NPs through the antioxidant system, the expression of genes related to melanin synthesis, and the synthesis of melanin.

Transcriptome analysis reveals the molecular mechanisms of Phragmites australis tolerance to CuO-nanoparticles and/or flood stress induced by arbuscular mycorrhizal fungi.

The molecular mechanism of arbuscular mycorrhizal fungi (AMF) in vertical flow constructed wetlands (VFCWs) for the purification of copper oxide nanoparticles (CuO-NPs) contaminated wastewater remains unclear. In this study, transcriptome analysis was used to explore the effect of AMF inoculation on the gene expression profile of Phragmites australis roots under different concentrations of CuO-NPs and/or flood stress. 551, 429 and 2281 differentially expressed genes (DEGs) were specially regulated by AMF under combined stresses of CuO-NPs and flood, single CuO-NPs stress and single flood stress, respectively. Based on the results of DEG function annotation and enrichment analyses, AMF inoculation under CuO-NPs and/or flood stress up-regulated the expression of a number of genes involved in antioxidant defense systems, cell wall biosynthesis and transporter protein, which may contribute to plant tolerance. The expression of 30 transcription factors (TFs) was up-regulated by AMF inoculation under combined stresses of CuO-NPs and flood, and 44 and 44 TFs were up-regulated under single CuO-NPs or flood condition, respectively, which may contribute to the alleviating effect of symbiosis on CuO-NPs and/or flood stress. These results provided a theoretical basis for enhancing the ecological restoration function of wetland plants for metallic nanoparticles (MNPs) by mycorrhizal technology in the future.

The positive effects of arbuscular mycorrhizal fungi inoculation and/or additional aeration on the purification efficiency of combined heavy metals in vertical flow constructed wetlands.

Inoculation with arbuscular mycorrhizal fungi (AMF) and additional aeration (AA), as two approaches to improve the functioning of treatment wetlands, can further promote the capacity of wetlands to purify pollutants. The extent to which, and mechanisms by which, AMF and AA purify wetlands polluted by combined heavy metals (HMs) are not well understood. In this study, the effects and mechanisms of AMF and/or AA on combined HMs removal in vertical flow constructed wetlands (VFCWs) with the Phragmites australis (reeds) were investigated at different HMs concentrations. The results showed that (1) AA improved the AMF colonization in VFCWs and AMF accumulated the combined HMs in their structures; (2) AMF inoculation and/or AA significantly promoted the reeds growth and antioxidant enzymes activities, thereby alleviating oxidative stress; (3) AMF inoculation and AA significantly enhanced the removal rates of Pb, Zn, Cu, and Cd under the stress of high combined HMs concentrations comparing to the control check (CK) treatment (autoclaved AMF inoculation and no aeration), which increased by 22.72%, 30.31%, 12.64%, and 50.22%, respectively; (4) AMF inoculation and/or AA significantly promoted the combined HMs accumulation in plant roots and substrates and altered the distribution of HMs at the subcellular level. We therefore conclude that AMF inoculation and/or AA in VFCWs improves the purification of combined HM-polluted water, and the VFCWs-reeds-AMF/AA associations exhibit great potential for application in remediation of combined HM-polluted wastewater.

Impact of Cold Weather on Setup Errors in Radiotherapy.

Objective: To investigate the influence of cold weather on setup errors of patients with chest and pelvic disease in radiotherapy. Methods: The image-guided data of the patients were collected from the Radiotherapy Center of Cancer Hospital Affiliated to Guangxi Medical University from October 2020 to February 2021. During this period, the cold weather days were December 15, 16, and 17, 2020, and January 7 and 8, 2021. For body fixation in radiotherapy, an integrated plate and a thermoplastic mold were employed in 18 patients with chest disease, while an integrated plate and a vacuum pad were applied in 19 patients with pelvic disease. All patients underwent cone beam computed tomography (CBCT) scans in the first five treatments and once a week thereafter. The obtained data were registered to the planning CT image to get the setup errors of the patient in the translational direction including X, Y, and Z axes and rotational direction including R X , R Y , and R Z . Then, the Mann-Whitney U test was performed. The expansion boundary values of the chest and pelvis were calculated according to the formula M PTV=2.5∑+0.7δ. Results: A total of 286 eligible results of CBCT scans were collected. There were 138 chest CBCT scans, including 26 taken in cold weather and 112 in usual weather, and 148 pelvic CBCT scans, including 33 taken in cold weather and 115 in usual weather. The X-, Y-, and Z-axis translational setup errors of patients with chest disease in the cold weather group were 0.16 (0.06, 0.32) cm, 0.25 (0.17, 0.52) cm, and 0.35 (0.21, 0.47) cm, respectively, and those in the usual weather group were 0.14 (0.08, 0.29) cm, 0.23 (0.13, 0.37) cm, and 0.18 (0.1, 0.35) cm, respectively. The results indicated that there was a statistical difference in the Z-axis translational error between the cold weather group and the usual weather group (U = 935.5; p=0.005 < 0.05), while there was no statistical difference in the rotational error between the two groups. The external boundary values of X, Y, and Z axes in the cold weather group were 0.57 cm, 0.92 cm, and 0.99 cm, respectively, and those in the usual weather group were 0.57 cm, 0.78 cm, and 0.68 cm, respectively. There was no significant difference in the translational and rotational errors of patients with pelvic disease between the cold weather group and the usual weather group (p < 0.05). The external boundary values of X, Y, and Z axes were 0.63 cm, 0.79 cm, and 0.68 cm in the cold weather group and 0.61 cm, 0.79 cm, and 0.61 cm in the usual weather group, respectively. Conclusion: The setup error of patients undergoing radiotherapy with their bodies fixed by an integrated plate and a thermoplastic mold was greater in cold weather than in usual weather, especially in the ventrodorsal direction.

The positive effects of inoculation using arbuscular mycorrhizal fungi and/or dark septate endophytes on the purification efficiency of CuO-nanoparticles-polluted wastewater in constructed wetland.

The extent to which, and mechanisms by which, arbuscular mycorrhizal fungi (AMF) and dark septate endophytes (DSE) purify wetlands polluted by metallic nanoparticles (metallic NPs) are not well understood. In this study, micro-vertical flow constructed wetlands (MVFCWs) with the Phragmites australis (reeds)-AMF/DSE symbiont were used to treat CuO nanoparticles (CuO-NPs)-polluted wastewater. The results showed that (1) the removal efficiencies of chemical oxygen demand (COD), total nitrogen (TN), and CuO-NPs in three inoculated groups significantly exceeded those in the control check (CK) groups by 28.94-98.72%, 16.63-47.66%, and 0.53-19.12%, respectively; (2) inoculation with AMF and/or DSE significantly promoted the growth, nutrient content, and photosynthesis of reeds, increased the osmoregulation substance content and antioxidant enzyme activities, and decreased the malondialdehyde and reactive oxygen species contents of reeds under CuO-NPs stress; (3) higher Cu accumulation and smaller transport coefficients were found in the inoculated groups than in the CK group; (4) inoculation with AMF and/or DSE changed the subcellular structure distribution and chemical form of Cu in reeds. We therefore conclude that inoculation with AMF and/or DSE in MVFCWs improves the purification of CuO-NPs-polluted wastewater, and the MVFCW-reeds-AMF/DSE associations exhibit great potential for application in remediation of metallic-NPs-polluted wastewater.

Diverse and abundant arbuscular mycorrhizal fungi in ecological floating beds used to treat eutrophic water.

An increasing number of investigations have shown the universal existence of arbuscular mycorrhizal fungi (AMF) in aquatic ecosystems. However, little is known about the accurate distribution and function of AMF inhabiting aquatic ecosystems, especially ecological floating beds (EFBs), which are constructed for the remediation of polluted water bodies. In this study, we collected root samples of Canna generalis, Cyperus alternifolius, and Eichhornia crassipes from three EFBs on two eutrophic lakes in Wuhan, China. We aimed to investigate the resources and distribution of AMF in EFBs using Illumina Mi-seq technology. A total of 229 operational taxonomic units (OTUs) and 21 taxa from 348,799 Glomeromycota sequences were detected. Glomus and Acaulospora were the most dominant and second most dominant genera of AMF in the three EFBs, respectively. Different aquatic plant species showed varying degrees of AMF colonization (3.83-71%), diversity (6-103 OTUs, 3-15 virtual taxa), and abundance (14-57,551 sequences). Low AMF abundance, but relatively high AMF diversity, was found in C. alternifolius, which is usually considered non-mycorrhizal. This finding indicated the high accuracy of Illumina sequencing. Our results also revealed a lognormal species abundance distribution that was observed across AMF taxa in the three plant species. The AMF community composition was closely related to nitrogen and phosphorus contents. Overall, our data show that EFBs harbor diverse and abundant AMF communities. Additionally, the AMF community composition is closely related to the water quality of eutrophic lakes treated by the EFBs, indicating the potential application of AMF in plant-based bioremediation of wastewater. KEYPOINTS: • Aquatic plants in EFBs harbor diverse (229 OTUs) and abundant (348,799 sequences) AMF. • Different plant species host different taxa of AMF. Cyperaceae, originally considered non-mycorrhizal, may in fact be a variable mycorrhizal plant family. • The AMF community composition in EFBs is closely related to nutrient concentrations (nitrogen and phosphorus).

Nitrogen removal from domestic wastewater using core-shell anthracite/Mg-layered double hydroxides (LDHs) in constructed wetlands.

To investigate the mechanism of nitrogen removal by anthracites and enhance the nitrogen removal efficiency in constructed wetland, three kinds of layered double hydroxides (MgFe-LDHs, MgCo-LDHs, MgAl-LDHs) were prepared by co-precipitation under alkaline conditions and coated in situ on the surface of anthracites to synthesize core-shell anthracites/Mg-LDHs composites. Experiments with different treatments (columns loaded with original anthracites and anthracite/Mg-LDH composites) were conducted to study the nitrogen removal efficiency of domestic wastewater in constructed wetlands. The results of nitrogen removal experiments showed that the anthracite/MgAl-LDH composite had the best performance with average removal rates of 53.69%, 72.91%, and 47.43% for TN, NH4+-N, and organic nitrogen, respectively. Modification changed the denitrification mode of the anthracites. The data of adsorption isothermal experiments were fitted better with the Freundlich model. The amount of ammonifier, nitrosobacteria, nitrobacter, and denitrifier on the surface of the Mg-LDH-modified anthracite was higher than that of the original anthracite. The performance of the anthracite in removing nitrogen was attributed to physical interception, chemical adsorption, and biological degradation. Moreover, the modified anthracites were superior to the original anthracite in the chemical adsorption and biodegradation, which indicated that coating the Mg-LDHs on the surface of common anthracite was a potential method to improve the nitrogen removal efficiency of domestic wastewater and to restore the eutrophic water body.

Effects of Aeration on the Formation of Arbuscular Mycorrhiza under a Flooded State and Copper Oxide Nanoparticle Removal in Vertical Flow Constructed Wetlands.

In this study, six vertical flow constructed wetlands (VFCWs) planted with Phragmites australis were operated at different aeration times (4 h day-1 and 8 h day-1), aeration modes (continuous and intermittent), and arbuscular mycorrhizal fungi (AMF) inoculation treatments (inoculation with Rhizophagus intraradices and no inoculation) to explore the effects of different aeration strategies on the formation of arbuscular mycorrhiza under a flooded state in VFCWs. In addition, these VFCWs were further used to treat copper oxide nanoparticle (CuO-NP) wastewater to evaluate the correlations among aeration, colonization, growth, and CuO-NP removal. The highest AMF 28S copy number (1.99×105) and colonization in reed roots, with values of 67%, 21%, and 1% for frequency (F%), intensity (M%), and arbuscule abundance (A%), were observed in the treatment with intermittent aeration for 4 h day-1. Aeration significantly increased the dissolved oxygen (DO) concentration and AMF colonization in VFCWs, thereby promoting plant growth and the purification of the CuO-NPs. However, excessive and continuous aeration had little positive effect on AMF colonization. This study provides a theoretical basis for the application of AMF for improving pollutant removal performance in constructed wetlands.

Microbial action and mechanisms for Cr(VI) removal performance by layered double hydroxide modified zeolite and quartz sand in constructed wetlands.

This study was conducted to evaluate the performance underlying the removal of hexavalent chromium Cr(VI) associated with Zn-layered double hydroxides (Zn-LDHs)-modified substrates utilized in simulated constructed wetlands (CWs) from a microbial perspective. To accomplish this, Zn-LDHs-modified substrates (zeolite and quartz sand (QS)) were synthesized at various Zn2+/Al3+ and Fe3+ molar ratios by co-precipitation under alkaline conditions. The experimental group was then compared with a control group to determine the microbial action responsible for Cr(VI) removal during the Cr(VI) removal experiments. The removal experiment revealed that the average Cr(VI) removal rates of the Zn-LDHs-modified substrates were superior to those of natural substrates. Subsequent evaluation of the microbial structure by Illumina high-throughput sequencing revealed that the relative abundance of Novosphingobium, Brevundimonas, Methylophilus, and Acidovorax related to Cr(VI) removal was relatively high in Zn-LDHs-modified substrates. Moreover, the extracellular polymeric substance (EPS) content was significantly influenced by the Zn-LDHs coating according to the relative microbial experiments. Similar trends were observed in enzyme activity. Taken together, these findings illustrated that the Zn-LDHs coating had a significant impact on microbial action, and the Cr(VI) removal efficiency of the Zn-LDHs-modified QS (zeolite) substrate was better than that of the natural substrate because of intracellular and extracellular removal mechanisms. Briefly, the microbial action of Zn-LDHs-modified QS played an important role in Cr(VI) removal, since the EPS content possessed the appropriate concentrations. Moreover, the microbial activity of ZnAl-LDHs-modified QS (zeolite) may have been higher than that of ZnFe-LDHs-modified QS (zeolite) because Al had a stronger promoting effect on Cr(VI) bio-removal than Fe. Therefore, the microbial Cr(VI) removal supported by ZnAl-LDHs-modified QS is a better choice for CWs.

Positive effects of Funneliformis mosseae inoculation on reed seedlings under water and TiO2 nanoparticles stresses.

TiO2 nanoparticles (TiO2NPs) is one of the most widely used nanomaterials. Arbuscular mycorrhizal fungi (AMF) are an important and widely distributed group of soil microorganisms, which promote the absorption of nutrients by host plants and increase their tolerance to contaminants. However, the effects and mechanisms of AMF on plant TiO2NPs tolerance in wetland habitats are not clear. In this experiment, under the conditions of three soil moisture contents (drought 50%, normal 70% and flooding 100%) and four TiO2NPs concentrations (0, 100, 200 and 500 mg kg-1), the effects of Funneliformis mosseae on the growth, antioxidant enzyme activities, osmotic substances and the absorption and accumulation of Ti in the Phragmites australis (reed) seedlings were studied. The results showed that the inoculation of F. mosseae under three moisture content conditions significantly increased the plant nutrition and root activities of reeds. Compared with the non-inoculated control, inoculation with F. mosseae increased the activities of antioxidant enzymes, the contents of chlorophyll, proline, soluble protein, and free amino acids, and significantly reduced the contents of malondialdehyde (MDA) and reactive oxygen species (ROS) of leaves. The accumulating ability of inoculated reeds to Ti was significantly higher than that of non-inoculated controls (P < 0.05), and inoculation of F. mosseae changed the distribution of Ti in reeds, increased the accumulation of Ti in roots. It's confirmed that inoculation of F. mosseae under three water conditions could improve the plant growth and nutrition, the activities of antioxidant enzymes, and enhance the reeds tolerance to TiO2NPs in this study.

Phosphorus removal and mechanisms by Zn-layered double hydroxide (Zn-LDHs)-modified zeolite substrates in a constructed rapid infiltration system.

This work presents novel materials, ZnFe-LDHs-modified (Zn2+ : Fe3+ molar ratio of 2 : 1 and 3 : 1) and ZnAl-LDHs-modified (Zn2+ : Al3+ molar ratio of 2 : 1 and 3 : 1) zeolites, which were synthesized under alkaline conditions via a co-precipitation method and coated in situ on original zeolites. The as-prepared LDHs-modified zeolites were used as substrates for a constructed rapid infiltration system (CRIS) to conduct purification experiments to investigate the phosphorus removal performance of all types of zeolites. The experimental results showed that the phosphorus removal rates of the Zn-LDHs-modified zeolites reached over 80%, which are superior to that of the original zeolites. Furthermore, isothermal adsorption and adsorption kinetic experiments were conducted to explore the adsorption mechanisms. The theoretical maximumadsorption capacities were efficiently enhanced owing to the Zn-LDHs coating strategy. Especially, that of the ZnFe-LDHs-modified (3 : 1) zeolites reached 434.78 mg kg-1, which is much higher than that of the original zeolites. Meanwhile, according to the fitting results of the adsorption kinetics experiments, it was found that the predominant adsorption type of the original zeolites was converted from intrinsically weak physical adsorption into more stable chemical adsorption by the Zn-LDHs coating. Furthermore, high-throughput sequencing was also exerted to uncover the phosphorus removal mechanism by microorganisms. The obtained results indicate that the relative abundance of Pseudomonas and Dechloromonas, which are closely related to phosphorus removal, effectively increased. Overall, the Zn-LDHs-modified zeolites improved the phosphorus removal performance efficiently and sustainably when applied in CRIS.

[Influencing Factors for Phosphorus Removal by Modified Bio-ceramic Substrates Coated with ZnAl-LDHs Synthesized by Different Modification Conditions].

Under different pH conditions, the hydrothermal and co-precipitation method was used to synthesize layered double hydroxides (LDHs) coated on bio-ceramic substrates with three different Zn2+/Al3+ molar ratios. Applying the original and six kinds of modified bio-ceramic substrates coated with ZnAl-LDHs (bio-ceramic/ZnAl-LDHs) in simulated vertical-flow constructed wetlands, experiments for phosphorus removal and isothermal adsorption were conducted to analyze the mechanism and effect of each synthesis factor. The results showed that ZnAl-LDHs (pH=11) had a more obvious effect on phosphorus removal, especially for bio-ceramic/ZnAl-LDHs (pH=11, 1:1), whose average removal rates of TP, TDP and SRP were enhanced over 70%. Its maximum adsorption capacity for phosphorus was three times higher than that of the original bio-ceramic. Both pH and Zn2+/Al3+ molar ratio affected the configuration and coating properties of bio-ceramic/ZnAl-LDHs at the time of synthesis, and pH was the main synthesis factor for phosphorus removal efficiency of bio-ceramic/ZnAl-LDHs. Through reasonable regulation of pH and Zn2+/Al3+ molar ratio when bio-ceramic/ZnAl-LDHs was synthesized, the phosphorus removal efficiency could be improved effectively.

Arbuscular mycorrhizal fungi in two vertical-flow wetlands constructed for heavy metal-contaminated wastewater bioremediation.

Over the last three decades, the presence of arbuscular mycorrhizal fungi (AMF) in wetland habitats had been proven, and their roles played in wetland ecosystems and potential functions in wastewater bioremediation technical installations are interesting issues. To increase knowledge on the functions of AMF in the plant-based bioremediation of wastewater, we constructed two vertical-flow wetlands planting with Phragmites australis and investigated AMF distribution in plant roots and their roles played in purification of wastewater polluted by heavy metals (HMs), utilizing the Illumina sequencing technique. A total of 17 operational taxonomic units (OTUs) from 33,031 AMF sequences were obtained, with Glomus being the most dominant. P. australis living in the two vertical-flow constructed wetlands (CWs) harbored diverse AMF comparable with the AM fungal communities in upland habitats. The AMF composition profiles of CW1 (vegetated with non-inoculated plants) and CW2 (vegetated with mycorrhizal plants inoculated with Rhizophagus intraradices) were significantly different. CW1 (15 OTUs) harbored more diverse AMF than CW2 (7 OTUs); however, CW2 harbored much more OTU13 than CW1. In addition, a zipf species abundance distribution (SAD), which might due to the heavy overdominance of OTU13, was observed across AM fugal taxa in P. australis roots of the two CWs. CW1 and CW2 showed high (> 70%) removal capacity of HMs. CW2 exhibited significant higher Cd and Zn removal efficiencies than CW1 (CK) (p = 0.005 and p = 0.008, respectively). It was considered that AMF might play a role in HM removal in CWs.

Enhanced removal performance of Cr(VI) by the core-shell zeolites/layered double hydroxides (LDHs) synthesized from different metal compounds in constructed rapid infiltration systems.

Nine kinds of LDHs were synthesized by the co-precipitation method under alkaline conditions with different combinations of trivalent metal compounds (FeCl3, AlCl3, CoCl3) and divalent metal compounds (CaCl2, MgCl2, ZnCl2), which were then coated in situ on the surface of zeolites to synthesize core-shell zeolites/LDHs composites. The zeolites before and after modification were characterized by SEM and X-ray fluorescence spectrometry. Using the different core-shell zeolites/LDHs and original zeolite substrates, the constructed rapid infiltration systems (CRIS) simulated test columns were set to treat the municipal sewage containing hexavalent chromium, Cr(VI). Isothermal adsorption tests were subsequently performed. The average removal efficiencies of the small-sized zeolites were much higher than those of the large-sized zeolites. For the small-sized zeolites, the Cr(VI) removal performances of the Mg-LDHs- and Al-LDHs-modified zeolite substrates were efficiently enhanced in particular, which could reach over 90%. And the removal rate of core-shell zeolites/ZnAl-LDHs reached 94.5%. Meanwhile, the maximum adsorption capacity of ZnAl-LDHs-modified zeolites could reach 51.0 mg/kg, indicating that the adsorption properties could be enhanced by ZnAl-LDHs coating. During the purification experiments, most of the LDHs-modified zeolites maintained their predominant chemical adsorption ability for the removal of Cr(VI). Therefore, the small-sized core-shell zeolites/ZnAl-LDHs composites could be used as potential substrates for the efficient removal of Cr(VI) in CRIS.