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1.
Res Vet Sci ; 91(3): 358-61, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20980035

RESUMO

Feline obesity generally results in aberrations to plasma metabolite levels, such as lipid concentrations and lipoprotein composition. This study sought to investigate the resultant effect of obesity on cholesterol lipoprotein composition and circulating adiponectin concentrations in cats. Plasma glucose, lipids (triglyceride, cholesterol and free fatty acid), insulin and adiponectin concentrations, and cholesterol lipoprotein composition were measured and compared between body condition score (BCS) determined normal healthy control and obese cats. Although the obese group demonstrated higher levels of plasma cholesterol, glucose, and triglycerides, as compared to healthy controls, the difference was insignificant thus indicating that the BCS determined obese cats may have been overweight and not morbidly obese. Plasma insulin levels were significantly higher (25-30%) versus healthy control animals thereby possibly hinting at the ensuing emergence of obesity induced insulin resistance. However, the BCS determined obese cat demonstrated a significant reduction (p<0.05) in plasma adiponectin concentration and a significant increase (p<0.05) in LDL-cholesterol % as compared to age matched healthy control animals. This would indicate that changes in plasma adiponectin concentration and cholesterol lipoprotein composition may be good early indicators of obesity in cats.


Assuntos
Adiponectina/sangue , Doenças do Gato/metabolismo , Lipoproteínas/sangue , Lipoproteínas/classificação , Obesidade/veterinária , Animais , Doenças do Gato/sangue , Gatos , Feminino , Lipoproteínas/metabolismo , Masculino , Obesidade/sangue , Obesidade/metabolismo
2.
Res Vet Sci ; 88(3): 458-60, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20080273

RESUMO

Metabolite, insulin and adiponectin concentrations and LDH, AST and ALT activities were measured in plasma of 142 client-owned cats (1-13years old, 16 breeds) to set up a new criterion of hypertriglyceridemia (hyper-TG) with increased plasma insulin concentrations for early diagnosis of lipid metabolism abnormality including obesity. 25 cats with over 165mg/dl of plasma triglyceride (TG) concentrations were decided as hyper-TG with increased plasma insulin concentrations, and prevalence of hyper-TG was 16.7% in young (1-6years old) and 18.3% in old (>7years old) cats examined. In the hyper-TG cats, their plasma TG concentrations increased to 6.6-7.4-fold of the values of control cats with 35-50mg/dl of plasma TG and their plasma cholesterol, FFA and insulin concentrations and LDH and ALT activities increased significantly, whereas their plasma adiponectin concentrations decreased significantly compared to those in the control cats. Hyper-TG cats with significantly increased body weights and plasma insulin and decreased plasma adiponectin seemed to be in early stage of obesity accompanying increased plasma insulin concentrations. Increased TG, insulin, LDH and ALT and decreased adiponectin values in plasma seemed to be key factors for diagnosis of lipid metabolism abnormality at early stage in cats.


Assuntos
Hipertrigliceridemia/metabolismo , Hipertrigliceridemia/veterinária , Insulina/sangue , Obesidade/veterinária , Envelhecimento , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Peso Corporal , Gatos , Colesterol/sangue , Hipercolesterolemia/epidemiologia , Hipercolesterolemia/veterinária , Hipertrigliceridemia/enzimologia , L-Lactato Desidrogenase/sangue , Obesidade/enzimologia , Obesidade/metabolismo , Valores de Referência
3.
Res Vet Sci ; 88(3): 394-9, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20060988

RESUMO

Miniature Schnauzers are the first canine breed, in the United States, reported to suffer from primary hyperlipidemia, but this has yet to be documented in other regions. Using over 900 canine plasma samples collected from over seven different veterinary clinics across Japan, the aim of this study was to compare plasma triglyceride (TG) and cholesterol concentrations between Miniature Schnauzers and other purebreeds in Japan. In addition, we investigated the influence of aging and sex on changes to hyperlipidemia incidence in purebred dogs. Our results indicated that both Miniature Schnauzers and Shetland sheepdogs in Japan exhibited remarkably high concentrations of plasma TG and total cholesterol, which are considered to be signs of hyperlipidemia, as compared to other purebred and mixed (Mongrel) canine breeds. Interestingly, the cause and conditions of primary hyperlipidemia in Miniature Schnauzers and Shetland sheepdogs might be different, with hypertriglyceridemia predominantly occurring with Miniature Schnauzers and hypercholesterolemia occurring in Shetland sheepdogs. However, with the influence of aging, the hyperlipidemia evolves into both hypercholesterolemia and hypertriglyceridemia in both groups indicating that the severity of hyperlipidemia positively correlates with aging. Gender differences were also observed with regards to severity. In fact, a higher severity was prevalent with female Miniature Schnauzers than their male counterparts whereas it was more balanced between genders for Shetland sheepdogs.


Assuntos
Doenças do Cão/epidemiologia , Cães/genética , Predisposição Genética para Doença/epidemiologia , Hiperlipidemias/veterinária , Envelhecimento , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Nitrogênio da Ureia Sanguínea , Colesterol/sangue , Creatinina/sangue , Doenças do Cão/genética , Feminino , Hiperlipidemias/epidemiologia , Hiperlipidemias/genética , Hipertrigliceridemia/epidemiologia , Hipertrigliceridemia/genética , Hipertrigliceridemia/veterinária , L-Lactato Desidrogenase/sangue , Masculino , Especificidade da Espécie , Triglicerídeos/sangue
4.
Vet Res Commun ; 33(4): 315-29, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18946721

RESUMO

Type 2 diabetes mellitus (DM) animal models have provided ample opportunity for investigating pathogenesis, as well as to evaluate novel treatment and prevention options for the disease. Because the domestic cat shares a similar environment with humans, it is also confronted with many similar risk factors for diabetes, such as physical inactivity and obesity. Obesity is a significant risk factor for diabetes in cats, and as such, the domestic cat may serve as an ideal model for investigating obesity induced insulin resistance. This study determined changes in insulin signaling genes within insulin sensitive tissues of obese felines. Quantitative RT-PCR was performed to determine mRNA levels of three important insulin signaling genes which have been implicated with insulin resistance: insulin receptor substrate (IRS)-1, IRS-2, and phosphatidylinositol 3'-kinase (PI3-K) p85alpha. Obese cats had significantly lower IRS-2 and PI3-K p85alpha mRNA levels in liver and skeletal muscle as compared to control cats. This down regulation of insulin signaling genes in obese cats mirrors that of obese humans and rodents suffering from insulin resistance. Interestingly, preprandial blood tests indicated that our obese cats were no different from control cats with regards to glucose tolerance and insulin resistance, thus indicating that the obese cats used in our study had a moderate level of obesity. Therefore, insulin signaling gene alterations were occurring in insulin sensitive tissues of moderately obese felines before glucose intolerance was clinically evident. As such, the monitoring of key insulin signaling genes may have some important diagnostic value to determine the risk level and degree of obesity induced insulin resistance.


Assuntos
Doenças do Gato/metabolismo , Proteínas Substratos do Receptor de Insulina/biossíntese , Resistência à Insulina/genética , Obesidade/metabolismo , Fosfatidilinositol 3-Quinases/biossíntese , Gordura Abdominal/enzimologia , Gordura Abdominal/metabolismo , Animais , Doenças do Gato/enzimologia , Doenças do Gato/genética , Gatos , Modelos Animais de Doenças , Expressão Gênica , Proteínas Substratos do Receptor de Insulina/genética , Fígado/enzimologia , Fígado/metabolismo , Masculino , Músculo Esquelético/enzimologia , Músculo Esquelético/metabolismo , Neutrófilos/enzimologia , Neutrófilos/metabolismo , Obesidade/enzimologia , Obesidade/genética , Fosfatidilinositol 3-Quinases/genética , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Transdução de Sinais
5.
Clin Exp Obstet Gynecol ; 35(1): 45-7, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18390080

RESUMO

PURPOSE OF INVESTIGATION: To determine if changes in basal body temperature (BBT) during the ovuratory phase are related to subsequent effects on pregnancy. METHODS: BBT records from 216 pregnant women in a spontaneous cycle or a clomiphene citrate cycle during a recent 6-year period were studied. The last day of low phase (LDLP) and the number of days until high phase (NDHP) were determined for all subjects. RESULTS: In the spontaneous cycle group, medium-cycle cases were most frequent and long-cycle cases were most frequent in the clomiphene cycle group. The NDHP ranged between one and three days in 82.8% of the subjects in the spontaneous cycle group and in 86.1% of the subjects in the clomiphene cycle group. CONCLUSIONS: Our findings demonstrate the importance of properly evaluating an NDPH of two or even three days in a BBT-based assessment of ovarian function in the ovulatory phase.


Assuntos
Temperatura Corporal/fisiologia , Ovulação/fisiologia , Clomifeno/uso terapêutico , Estudos de Coortes , Feminino , Fármacos para a Fertilidade Feminina/uso terapêutico , Fase Folicular/fisiologia , Humanos , Fase Luteal/fisiologia , Gravidez
6.
J Nucl Med ; 41(1): 85-92, 2000 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-10647609

RESUMO

UNLABELLED: The goals of this study were to correlate FDG uptake with cell proliferation and cellular density in non-small cell lung cancer. METHODS: Thirty-one patients with 32 non-small cell lung cancers were examined with FDG PET. For semiquantitative analysis, standardized uptake values (SUVs) were calculated. All patients underwent thoracotomy within 4 wk after the FDG PET study. Cell proliferation was immunohistochemically assessed as the relative number of cells expressing the proliferating cell nuclear antigen ([PCNA] labeling index). Cellular density was also evaluated using light microscopy. RESULTS: SUVs correlated significantly with PCNA labeling index (r = 0.740; P < 0.0001) but only weakly with cellular density (r = 0.392; P = 0.0266). High FDG uptake correlated with high PCNA expression. The PCNA labeling index and SUVs were significantly lower in bronchioloalveolar carcinomas (n = 8) (12.3 +/- 9.45% and 1.45 +/- 0.76, respectively) than in nonbronchioloalveolar carcinomas (n = 19) (33.5 +/- 21.8%, P = 0.015, and 3.75 +/- 1.93, P = 0.003, respectively). However, no significant differences in cellular density were seen between bronchioloalveolar carcinomas and nonbronchioloalveolar carcinomas. CONCLUSION: FDG uptake is related to cell proliferation rather than to the cellular density of non-small cell lung cancer.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/diagnóstico por imagem , Radioisótopos de Flúor , Fluordesoxiglucose F18 , Neoplasias Pulmonares/diagnóstico por imagem , Compostos Radiofarmacêuticos , Tomografia Computadorizada de Emissão , Carcinoma Pulmonar de Células não Pequenas/patologia , Divisão Celular , Feminino , Humanos , Pulmão/diagnóstico por imagem , Pulmão/patologia , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Antígeno Nuclear de Célula em Proliferação/análise
7.
Nihon Kokyuki Gakkai Zasshi ; 37(1): 61-6, 1999 Jan.
Artigo em Japonês | MEDLINE | ID: mdl-10087879

RESUMO

A 52-year-old woman who had undergone a partial mastectomy 1 year earlier because of benign phyllodes tumor was admitted because of dry cough and abnormal chest radiograph findings. Chest computed tomograms demonstrated multiple thin-walled cavities and nodules. Clinical examinations and transbronchial biopsy specimens failed to provide a conclusive diagnosis. However, the pulmonary thin-walled cavities enlarged, and a nodular shadow revealed cavitary formation. An open lung biopsy was performed to diagnose the pulmonary lesions. Although biopsy specimens disclosed the infiltration of poorly differentiated adenocarcinoma cells in pleura and pulmonary parenchyma, no primary site was detected. The patient did not respond to systemic chemotherapy (CDDP and VP-16), and died of respiratory failure due to advanced pulmonary metastasis. Autopsy demonstrated marked tumor invasion of the lungs, myocardium, and bone. We analyzed malignant cells in lung tissues at autopsy by immunohistochemistry, and found identical malignant cells in surgical samples obtained during the patients earlier mastectomy. A diagnosis of pulmonary metastasis from malignant phyllodes tumor of the breast was made. Thin walled cavitary lesions from malignant phyllodes tumor are rare; however, pulmonary metastasis of malignant phyllodes tumor should be considered one disease that exhibits thin-walled cavities as a radiographic manifestation.


Assuntos
Adenocarcinoma/secundário , Neoplasias da Mama/patologia , Neoplasias Pulmonares/secundário , Pulmão/patologia , Tumor Filoide/secundário , Adenocarcinoma/diagnóstico por imagem , Feminino , Humanos , Pulmão/diagnóstico por imagem , Neoplasias Pulmonares/diagnóstico por imagem , Pessoa de Meia-Idade , Tumor Filoide/diagnóstico por imagem , Radiografia
8.
Nihon Kokyuki Gakkai Zasshi ; 36(10): 871-4, 1998 Oct.
Artigo em Japonês | MEDLINE | ID: mdl-9893429

RESUMO

A 69-year-old woman was admitted because of persistent productive cough, low-grade fever and abnormal pulmonary infiltrates. Chest roentgenograms revealed right pulmonary cavitary lesions and infiltrates in both upper pulmonary fields. Digital subtraction bronchography (DSBG) was performed to detect for the presence of pulmonary cavitary lesions. Double-contrast DSBG images clearly revealed marked bronchiectasis in the right pulmonary cavity with aspergilloma in the cavitary lesions. Aspergilloma was not obvious in routine chest roentgenograms and computed tomograms. Asperillus fumigatus was cultured from sputum materials, and aspergilloma of the pulmonary cavity was clinically diagnosed. The infiltrative lesions were thought to be associated with invasive aspergillosis because the patient exhibited persistent low-grade fever and positive inflammatory reactions. Anti-fungal chemotherapy was highly effective in treating these lesions. DSBG is a new, less-invasive bronchographic technique for the evaluation of bronchial trees, and in this case proved useful for the detection of small aspergilloma in pulmonary cavities.


Assuntos
Aspergilose/diagnóstico por imagem , Aspergillus fumigatus , Broncografia/métodos , Pneumopatias Fúngicas/diagnóstico por imagem , Idoso , Bronquiectasia/diagnóstico por imagem , Feminino , Humanos
9.
Gan To Kagaku Ryoho ; 20(11): 1638-41, 1993 Aug.
Artigo em Japonês | MEDLINE | ID: mdl-7690538

RESUMO

The concomitant administration of Sizofiran (a macrophage activator) and rG-CSF (which promotes neutrophil proliferation and activation) caused marked activation of intraperitoneal antitumor immunity. It promoted the induction of IL-2 receptor expression as well as the proliferation and activation of neutrophils, and also caused an increase in LAK and NK activity, which resulted in a clinical antitumor effect. Therefore, this concomitant regimen was found to be useful as maintenance therapy for ovarian cancer.


Assuntos
Fator Estimulador de Colônias de Granulócitos/administração & dosagem , Neoplasias Ovarianas/terapia , Sizofirano/administração & dosagem , Esquema de Medicação , Feminino , Humanos , Infusões Parenterais , Células Matadoras Ativadas por Linfocina/imunologia , Células Matadoras Naturais/imunologia , Neoplasias Ovarianas/imunologia , Receptores de Interleucina-2/imunologia
11.
Gan To Kagaku Ryoho ; 20(8): 1071-4, 1993 Jun.
Artigo em Japonês | MEDLINE | ID: mdl-7685585

RESUMO

Preoperative 5-drug-combined intraarterial infusion chemotherapy using carboplatin as a main component was performed in a case of uterine cervical squamous cell carcinoma of Stage IIa. The regimen employed was as follows: carboplatin (100 mg/m2) on Day 4 and 5, vincristine (0.6 mg/m2) on Day 1, peplomycin (5 mg/body) on Day 1, 2, and 3, methotrexate (5 mg/m2) on Day 2 and 3, and doxorubicin (15 mg/m2) on Day 4. These drugs were administered into the right inguinal region through a residual catheter at 3-week intervals prior to operation. After completion of 3 courses, the patient achieved a CR confirmed by pathological analysis and diagnostic imaging followed by extended radical hysterectomy. Side effects observed, especially renal disturbance and myelosuppression, were all tolerable. Intraarterial infusion chemotherapy using carboplatin was thus suggested to be useful against malignant tumors in the gynecological field.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Carboplatina/administração & dosagem , Carcinoma de Células Escamosas/tratamento farmacológico , Neoplasias do Colo do Útero/tratamento farmacológico , Bleomicina/administração & dosagem , Carcinoma de Células Escamosas/cirurgia , Quimioterapia Adjuvante , Doxorrubicina/administração & dosagem , Esquema de Medicação , Feminino , Humanos , Histerectomia , Infusões Intra-Arteriais , Metotrexato/administração & dosagem , Pessoa de Meia-Idade , Peplomicina , Cuidados Pré-Operatórios , Indução de Remissão , Neoplasias do Colo do Útero/cirurgia , Vincristina/administração & dosagem
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