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2.
Nat Commun ; 14(1): 2683, 2023 05 09.
Artigo em Inglês | MEDLINE | ID: mdl-37160917

RESUMO

Many secretory enzymes acquire essential zinc ions (Zn2+) in the Golgi complex. ERp44, a chaperone operating in the early secretory pathway, also binds Zn2+ to regulate its client binding and release for the control of protein traffic and homeostasis. Notably, three membrane transporter complexes, ZnT4, ZnT5/ZnT6 and ZnT7, import Zn2+ into the Golgi lumen in exchange with protons. To identify their specific roles, we here perform quantitative Zn2+ imaging using super-resolution microscopy and Zn2+-probes targeted in specific Golgi subregions. Systematic ZnT-knockdowns reveal that ZnT4, ZnT5/ZnT6 and ZnT7 regulate labile Zn2+ concentration at the distal, medial, and proximal Golgi, respectively, consistent with their localization. Time-course imaging of cells undergoing synchronized secretory protein traffic and functional assays demonstrates that ZnT-mediated Zn2+ fluxes tune the localization, trafficking, and client-retrieval activity of ERp44. Altogether, this study provides deep mechanistic insights into how ZnTs control Zn2+ homeostasis and ERp44-mediated proteostasis along the early secretory pathway.


Assuntos
Complexo de Golgi , Proteostase , Humanos , Homeostase , Transporte Biológico , Bioensaio , Proteínas de Membrana , Chaperonas Moleculares
3.
Cell Chem Biol ; 27(12): 1521-1531.e8, 2020 12 17.
Artigo em Inglês | MEDLINE | ID: mdl-32997976

RESUMO

Fluorescent Zn2+ probes used for the quantitative analysis of labile Zn2+ concentration ([Zn2+]) in target organelles are crucial for understanding the role of Zn2+ in biological processes. Although several fluorescent Zn2+ probes have been developed to date, there is still a lack of consensus concerning the [Zn2+] in intracellular organelles. In this study, we describe the development of ZnDA-1H, a small-molecule fluorescent probe for Zn2+, which exhibits less pH sensitivity, high Zn2+ selectivity, and large fluorescence enhancement upon binding to Zn2+. Through protein labeling technology, ZnDA-1H was precisely targeted in various intracellular organelles, such as the nucleus, mitochondria, endoplasmic reticulum, and Golgi apparatus. ZnDA-1H exhibited a reversible fluorescence response toward labile Zn2+ in these organelles in live cells. Using this probe, the [Zn2+] in the Golgi apparatus was estimated to be 25 ± 1 nM, suggesting that labile Zn2+ plays a physiological role in the secretory pathway.


Assuntos
Corantes Fluorescentes/química , Corantes Fluorescentes/metabolismo , Complexo de Golgi/metabolismo , Microscopia de Fluorescência , Zinco/metabolismo , Células HeLa , Humanos , Coloração e Rotulagem
4.
Nat Commun ; 10(1): 603, 2019 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-30723194

RESUMO

Zinc ions (Zn2+) are imported into the early secretory pathway by Golgi-resident transporters, but their handling and functions are not fully understood. Here, we show that Zn2+ binds with high affinity to the pH-sensitive chaperone ERp44, modulating its localization and ability to retrieve clients like Ero1α and ERAP1 to the endoplasmic reticulum (ER). Silencing the Zn2+ transporters that uptake Zn2+ into the Golgi led to ERp44 dysfunction and increased secretion of Ero1α and ERAP1. High-resolution crystal structures of Zn2+-bound ERp44 reveal that Zn2+ binds to a conserved histidine-cluster. The consequent large displacements of the regulatory C-terminal tail expose the substrate-binding surface and RDEL motif, ensuring client capture and retrieval. ERp44 also forms Zn2+-bridged homodimers, which dissociate upon client binding. Histidine mutations in the Zn2+-binding sites compromise ERp44 activity and localization. Our findings reveal a role of Zn2+ as a key regulator of protein quality control at the ER-Golgi interface.


Assuntos
Proteínas de Membrana/metabolismo , Chaperonas Moleculares/metabolismo , Via Secretória , Zinco/metabolismo , Aminopeptidases/metabolismo , Sítios de Ligação/genética , Proteínas de Transporte de Cátions/genética , Proteínas de Transporte de Cátions/metabolismo , Cristalografia por Raios X , Retículo Endoplasmático/metabolismo , Complexo de Golgi/metabolismo , Células HeLa , Células Hep G2 , Humanos , Glicoproteínas de Membrana/metabolismo , Proteínas de Membrana/química , Proteínas de Membrana/genética , Antígenos de Histocompatibilidade Menor/metabolismo , Chaperonas Moleculares/química , Chaperonas Moleculares/genética , Oxirredutases/metabolismo , Ligação Proteica , Conformação Proteica , Multimerização Proteica , Controle de Qualidade , Interferência de RNA , Zinco/química
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