RESUMO
In China, most maize used for animal diets is stored for long periods. We examined the effects of dietary aged maize on growth performance, nutrients utilization, and serum metabolites in broilers. A total of 270 healthy 1-day-old male Cobb broilers were assigned randomly into three treatments groups and fed maize stored for different times (24 days, M0; 18 months, M18; 36 months, M36). Growth performance was examined at 21 and 42 days of age. Nutrient digestibility was studied on days 18-21 and 38-41. At day 42, blood samples were collected for serum metabolite analysis. Dietary aged maize significantly affected the feed to gain ratio, total starch digestibility, and apparent metabolizable energy (p < 0.05). Compared with the M0 group, 39 and 144 differential metabolites were observed in the M18 and M36 groups, respectively, whereas 56 differential metabolites were identified between the M18 and M36 groups. Pathway analysis indicated that the main altered pathways were clustered into lipid metabolism in M18, and lipid and glucose metabolism in M0 and M36, respectively. In conclusion, negative effects were observed for both new harvested maize and maize stored for 36 months; maize stored for 18 months may improve broiler performance.
Assuntos
Galinhas , Zea mays , Animais , Masculino , Digestão , Ração Animal/análise , Dieta , NutrientesRESUMO
Surface-enhanced Raman spectroscopy (SERS) is a powerful technique for the discrimination, identification, and potential quantification of certain compounds/organisms. However, its real application is challenging due to the multiple interference from the complicated detection matrix. Therefore, selective/specific detection is crucial for the real application of SERS technique. We summarize in this review five selective/specific detection techniques (chemical reaction, antibody, aptamer, molecularly imprinted polymers and microfluidics), which can be applied for the rapid and reliable selective/specific detection when coupled with SERS technique.