Essential oil from Chimonanthus nitens Oliv. Leaves ameliorate inflammation and oxidative stress in LPS-induced ALI through NF-κB and Nrf2 signaling pathways.

ETHNOPHARMACOLOGICAL RELEVANCE: Initial investigative research indicated that the essential oil from Chimonanthus nitens Oliv. Leaves (CLO) significantly reduces lung tissues inflammation and effectively repairs Acute lung injury (ALI) mice model. However, the mechanism underlying is not clear, and the impacts of CLO on oxidative stress require further investigation. AIM OF THE STUDY: The purpose of the experiment was to validate the influence of CLO in ALI model mice, as well as its potential mechanisms. MATERIALS AND METHODS: Lipopolysaccharide-induced establishment of the A549 cell inflammation model, and ALI mice model was established by intrathecal administration of LPS. RESULTS: CLO significantly reduced the release of inflammatory cytokines in A549 cells, lowered MDA and ROS levels, and enhanced SOD activity. Animal experiment results showed that CLO dramatically decreased white blood cell count, the expression of inflammatory cytokines, and the destruction of alveolar structures. CLO enhances the activity of antioxidant enzymes. Western Blot and q-PCR analyses have revealed that the mechanism of CLO is correlation with the NF-κB and Nrf2 signaling pathways in cellular and animal models. Pathway inhibitor experiments indicated that there might be functional crosstalk between these two pathways. CONCLUSIONS: CLO may regulate inflammation and oxidative stress in LPS-induced ALI through NF-κB and Nrf2 signaling pathways. This finding could be novel in the pharmacological treatment of ALI.

Advanced analytical techniques for authenticity identification and quality evaluation in Essential oils: A review.

Essential oils (EO), secondary metabolites of plants are fragrant oily liquids with antibacterial, antiviral, anti-inflammatory, anti-allergic, and antioxidant effects. They are widely applied in food, medicine, cosmetics, and other fields. However, the quality of EOs remain uncertain owing to their high volatility and susceptibility to oxidation, influenced by factors such as the harvesting season, extraction, and separation techniques. Additionally, the huge economic value of EOs has led to a market marked by widespread and varied adulteration, making the assessment of their quality challenging. Therefore, developing simple, quick, and effective identification techniques for EOs is essential. This review comprehensively summarizes the techniques for assessing EO quality and identifying adulteration. It covers sensory evaluation, physical and chemical property evaluation, and chemical composition analysis, which are widely used and of great significance for the quality evaluation and adulteration detection of EOs.

An integrated approach utilizing raman spectroscopy and chemometrics for authentication and detection of adulteration of agarwood essential oils.

Agarwood is a precious aromatic plant which has good pharmacological effects such as antidepressant and sedation. It also has good ornamental and collection value. However, due to it is long and complex production process, the output of agarwood essential oils (AEOs) is scarce, so the price is expensive, the quality is uneven, and the adulteration events is endless. From the commercial and pharmaceutical point of view, the authenticity and quality of the commercial products labeled as AEOs is very important. This paper tested the applicability of Raman spectroscopy combined with chemometrics in classification and authenticity identification of AEOs. In this study, Raman spectroscopy and principal component analysis (PCA) combined with partial least square discriminant analysis (PLS-DA) were used to comprehensively evaluate AEOs from different geographical origins and/or extracted by different methods which showed different characteristic bands. The characteristic component of AEOs, chromone derivatives, and two commonly used adulterants were also detected. These characteristic bands provide spectrum information of AEO samples and reference materials, which can be used as Raman spectral markers for the qualitative identification of AEOs. This study can provide a novel, fast and convenient method for identification of AEOs.