RESUMO
The eye irritation potential of drug candidates or pharmaceutical ingredients should be evaluated if there is a possibility of ocular exposure. Traditionally, the ocular irritation has been evaluated by the rabbit Draize test. However, rabbit eyes are more sensitive to irritants than human eyes, therefore substantial level of false positives are unavoidable. To resolve this species difference, several three-dimensional human corneal epithelial (HCE) models have been developed as alternative eye irritation test methods. Recently, we introduced a new HCE model, MCTT HCE(TM) which is reconstructed with non-transformed human corneal cells from limbal tissues. Here, we examined if MCTT HCE(TM) can be employed to evaluate eye irritation potential of solid substances. Through optimization of washing method and exposure time, treatment time was established as 10 min and washing procedure was set up as 4 times of washing with 10 mL of PBS and shaking in 30 mL of PBS in a beaker. With the established eye irritation test protocol, 11 solid substances (5 non-irritants, 6 irritants) were evaluated which demonstrated an excellent predictive capacity (100% accuracy, 100% specificity and 100% sensitivity). We also compared the performance of our test method with rabbit Draize test results and in vitro cytotoxicity test with 2D human corneal epithelial cell lines.
RESUMO
We present analytic expressions for time-dependent space charge field and two wave mixing gain under the external applied field accompanying the grating translation. The variations of complex space charge field are analyzed in a complex plane, and the explicit expressions for the resonance and optimum frequencies (or moving grating velocities), which maximize the magnitude and imaginary part of space charge field, respectively, are also obtained. In addition, two wave mixing experiment was conducted with a grating translation technique without the external applied field in a BaTiO(3) crystal and the transient behaviors of measured gain look like damped harmonic oscillations, showing excellent agreement with the theory for the entire time range.
RESUMO
We investigated the diffraction properties of dynamic holograms recorded in porphyrin:Zn doped nematic liquid crystals (NLCs) under the influence of an applied dc electric field for various conditions of the grating period, the writing beam intensity and the applied electric field. We also derived an analytic expression for diffraction efficiency from NLCs material equations and torque balance equations and compared the experimental results with the theory, revealing excellent agreement.