RESUMO
Suncus murinus has been identified as the host for Seoul virus (SEOV). Here, we report the complete genome sequence of SEOV strain Fj372/2013, which was isolated from the lung tissue of Suncus murinus in the Fujian Province of China. A mutation A38C was observed in an open reading fragment of the middle segment.
RESUMO
To gain more insights into epidemiologic characteristics and genotype of hantavirus in Apodemus agrarius in Changbai Area. Complete hantavirus S segment sequences were amplified by RT-PCR and sequenced. The phylogenetic trees were constructed for analysis of genetic characters of hantavirus. A total of 58 Apodemus agrarius were trapped in the epidemic areas, and complete hantavirus S segment sequences were obtained from 4 lung samples of these rodents (6. 90%0). Phylogenetic analysis of the four S segment sequences indicated that all viruses isolated from Apodemu sagrarius were closely related to genotype 6 of Hantaan virus (95. 8%-96. 3%, nucleotide identity; 98. 6%-99. 5%, amino acid identity), all of them had a specific S387 different from other genotypes of Hantaan virus.
Assuntos
Reservatórios de Doenças/virologia , Infecções por Hantavirus/veterinária , Murinae/virologia , Orthohantavírus/genética , Doenças dos Roedores/virologia , Proteínas Virais/genética , Animais , Sequência de Bases , China/epidemiologia , DNA Complementar/química , DNA Complementar/genética , Genótipo , Orthohantavírus/classificação , Orthohantavírus/isolamento & purificação , Infecções por Hantavirus/epidemiologia , Infecções por Hantavirus/virologia , Pulmão/virologia , Filogenia , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNARESUMO
Wohlfahrtiimonas chitiniclastica bacilli that live in the larvae of a parasitic fly were recently isolated and are speculated to be the cause of fulminant sepsis. Here we report and analyze the complete genome sequence of Wohlfahrtiimonas chitiniclastica strain SH04. No complete genome sequence of a Wohlfahrtiimonas chitiniclastica isolate has been documented previously.
RESUMO
Amur virus was recently identified as the causative agent of hemorrhagic fever with renal syndrome. Here we report the complete genome sequence of an Amur virus isolated from Apodemus peninsulae in Northeastern China. The sequence information provided here is critical for the molecular epidemiology and evolution of Amur virus in China.
Assuntos
Genoma Viral , Vírus Hantaan/genética , Murinae/virologia , Animais , China , Evolução Molecular , Dados de Sequência MolecularRESUMO
Seoul virus (SEOV) is responsible for 25% of cases of hemorrhagic fever with renal syndrome in Asia. Here we report the complete genome of strain DPRK08. The sequence information provided here is useful for understanding the molecular character of SEOV in the Democratic People's Republic of Korea (DPRK) and the circulation of SEOV in East Asia.
Assuntos
Genoma Viral , Vírus Seoul/genética , Animais , Dados de Sequência Molecular , Ratos , República da CoreiaRESUMO
BACKGROUND: To acquire the recombinant human monoclonal antibodies and IgG to adeno-associated virus type 2 (AAVs-2). METHODS: Construct and pan human Fab antibody library to AAVs-2 was established from normal volunteer donors by using phage display technology and secreted expression in E.Coli system. The positive Fab clones were selected and characterized through ELISA and immunofluorescent assay, and then the heavy and light chain were sequenced. The gene of light chain and heavy chain Fd fragment of recombinant mAb were inserted into baculovirus expression vector pAC-L-Fc and construct expression vectors of intact IgG, then transfected insert sf-9 cell secreted expression in Baculovirus/Insert system. Immunoprecipitation test was used to detect its recognizing region. RESULTS: One clone named AAVs-31 showed positive responses in ELISA and IFA, the Fab was composed of gamma chain and kappa chain IgG was positive in ELISA and IFA. The IgG failed to detect nonassembled or denatured capsid proteins, but recognized the AAVs-2 stock from immunoprecipitation test. CONCLUSION: The authors isolated a clone of Fab and IgG to adeno-associated virus type 2 by phage display technology, they perhaps recognize an epitope which is formed during capsid assembly.
