Neuromuscular disease auxiliary diagnosis using a portable magnetomyographic system.

Objective. The measurement of electromyography (EMG) signals with needle electrodes is widely used in clinical settings for diagnosing neuromuscular diseases. Patients experience pain during needle EMG testing. It is significant to develop alternative diagnostic modalities.Approach. This paper proposes a portable magnetomyography (MMG) measurement system for neuromuscular disease auxiliary diagnosis. Firstly, the design and operating principle of the system are introduced. The feasibility of using the system for auxiliary diagnosis of neuromuscular diseases is then studied. The magnetic signals and needle EMG signals of thirty subjects were collected and compared.Main results. It is found that the amplitude of muscle magnetic field signal increases during mild muscle contraction, and the signal magnitudes of the patients are smaller than those of normal subjects. The diseased muscles tested in the experiment can be distinguished from the normal muscles based on the signal amplitude, using a threshold value of 6 pT. The MMG diagnosis results align well with the needle EMG diagnosis. In addition, the MMG measurement indicates that there is a persistence of spontaneous activity in the diseased muscle.Significance.The experimental results demonstrate that it is feasible to auxiliary diagnose neuromuscular diseases using the portable MMG system, which offers the advantages of non-contact and painless measurements. After more in-depth, systematic, and quantitative research, the portable MMG could potentially be used for auxiliary diagnosis of neuromuscular diseases. The clinical trial registration number is ChiCTR2200067116.

Microfluidic magnetic detection system combined with a DNA framework-mediated immune-sandwich assay for rapid and sensitive detection of tumor-derived exosomes.

Tumor-derived circulating exosomes (TDEs) are being pursued as informative and noninvasive biomarkers. However, quantitatively detecting TDEs is still challenging. Herein, we constructed a DNA tetrahedral-structured probe (TSP)-mediated microfluidic magnetic detection system (µFMS) to provide a rapid and sensitive platform for analyzing TDEs. CD63 aptamer-modified Fe3O4 magnetic nanoparticles (MNPs) were constructed to form magnetic nano-report probes (MNRs). The microfluidic chips were fabricated from glass functionalized with DNA TSP-modified aldehyde groups and a PDMS layer designed with serpentine microchannels. An induction coil-based magnetic detector was used to measure the magnetic signal. The linear dynamic range of the µFMS system for TDE assays was 1.98 × 103-1.98 × 107 particles/mL with a limit of detection of 1.98 × 103 particles/mL in PBS. There was no significant difference in TDE detection between the simulated serum and PBS, which indicated the feasibility of the constructed µFMS system for TDE analysis in complex biological systems. In terms of cost, reaction time and operation procedure, this µFMS has the potential to be developed as a clinical point-of-care testing tool for cancer diagnosis and therapeutics.

Resolution Enhanced Array ECT Probe for Small Defects Inspection.

It is a continual and challenging problem to detect small defects in metallic structures for array eddy current testing (ECT) probes, which require the probe to have ultra-high resolution and sensitivity. However, the spatial resolution of an ECT array probe is limited by the size of the induction coils. Even if it is possible to increase the spatial resolution by using smaller coils, the sensitivity of the sensor also decreases. To obtain finer spatial resolution without sacrificing sensitivity, this paper proposes a resolution enhanced ECT array probe with four rows of coils attached to a flexible printed circuit board (FPCB). The distance between each two adjacent coils in a row is 2 mm and the position of each row is offset by 0.5 mm along the horizontal direction related to its prior row. The outputs of the four rows are aligned and interpolated in a line, and in this way the image resolution of the probe is increased to 0.5 mm. The probe is configured to operate with the differential setting, namely two differential coils operate simultaneously at each time. The currents in the two coils can be controlled to have the same flowing direction or opposite flowing direction, resulting in different distributions of the induced eddy current and two sets of output images. A patch-image model and an image fusion method based on discrete wavelet transforms are employed to suppress the noise and highlight the defects' indications. Experimental results show that small defects with dimensions as small as length × width × depth = 1 mm × 0.1 mm × 0.3 mm on a 304 stainless-steel sample can be detected from the fused image, demonstrating that the probe has super sensitivity for small defects inspection.

Tunnel Magnetoresistance Sensor with AC Modulation and Impedance Compensation for Ultra-Weak Magnetic Field Measurement.

Tunnel magnetoresistance (TMR) is a kind of magnetic sensor with the advantages of low cost and high sensitivity. For ultra-weak and low-frequency magnetic field measurement, the TMR sensor is affected by the 1/f noise. This paper proposes an AC modulation method with impedance compensation to improve the performance. The DC and AC characteristics of the sensors were measured and are presented here. It was found that both the equivalent resistance and capacitor of the sensors are affected by the external magnetic field. The TMR sensors are connected as a push-pull bridge circuit to measure the magnetic field. To reduce the common-mode noise, two similar bridge circuits form a magnetic gradiometer. Experimental results show that the sensor's sensitivity in the low-frequency range is obviously improved by the modulation and impedance compensation. The signal-to-noise ratio of the sensor at 1 Hz was increased about 25.3 dB by the AC modulation, impedance compensation, and gradiometer measurement setup. In addition, the sensitivity of the sensor was improved from 165.2 to 222.1 mV/V/mT. Ultra-weak magnetic signals, namely magnetocardiography signals of two human bodies, were measured by the sensor in an unshielded environment. It was seen that the R peak of MCG can be clearly visualized from the recorded signal.

Stk and Stp1 participate in Streptococcus suis serotype 2 pathogenesis by regulating capsule thickness and translocation of certain virulence factors.

Eukaryotic-like serine/threonine protein kinase (eSTK) and phosphatase (eSTP) play multiple roles in pathogenesis of many Gram-positive bacteria. eSTK (Stk) and eSTP (Stp1) of Streptococcus suis serotype 2 (S. suis 2) have also been reported to be virulence-associated, but their roles and underlying mechanisms in S. suis 2 pathogenesis require further investigation. We constructed mutants of stk or stp1 deletion using the virulent S. suis 2 isolate 05ZYH33 as the parental strain. Both Δstk and Δstp1 mutants showed abnormal cell division shown as increased chain length. This might be due to regulation by Stk and Stp1 of the phosphorylation status of the bacterial division protein DivIVA. Both mutants showed increased adhesion but reduced invasion to epithelial and endothelial cells. The two mutants were more readily phagocytosed by murine RAW264.7 macrophages. Western blotting revealed that GAPDH (glyceraldehyde-3-phosphate dehydrogenase), an important adhesin of S. suis, was significantly increased in the surface-associated and secreted fractions of the two mutant strains. Because increased adhesion of the mutant strains Δstk and Δstp1 to endothelial cells could be significantly inhibited by anti-GAPDH serum, we suppose that aberrant translocation of GAPDH due to deletion of the stk or stp1 gene contributed to increased interaction with host cells. The Δstk mutant showed reduced survival in macrophages, while the Δstp1 mutant showed increased survival probably as a result of increased capsule thickness. Enhanced hemolytic activity of the Δstk mutant could be due to increased secretion of suilysin. Both mutants exhibited reduced survival in pig whole blood and attenuated virulence to mice. Taken together, these results suggest that Stk and Stp1 can modulate S. suis cell division by post-translational modification of DivIVA, and regulate translocation of certain virulence factors, thereby benefiting its pathogenicity by compromising its interactions with the host.

Optimization and Validation of Rotating Current Excitation with GMR Array Sensors for Riveted Structures Inspection.

In eddy current non-destructive testing of a multi-layered riveted structure, rotating current excitation, generated by orthogonal coils, is advantageous in providing sensitivity to defects of all orientations. However, when used with linear array sensors, the exciting magnetic flux density ( B x ) of the orthogonal coils is not uniform over the sensor region, resulting in an output signal magnitude that depends on the relative location of the defect to the sensor array. In this paper, the rotating excitation coil is optimized to achieve a uniform B x field in the sensor array area and minimize the probe size. The current density distribution of the coil is optimized using the polynomial approximation method. A non-uniform coil design is derived from the optimized current density distribution. Simulation results, using both an optimized coil and a conventional coil, are generated using the finite element method (FEM) model. The signal magnitude for an optimized coil is seen to be more robust with respect to offset of defects from the coil center. A novel multilayer coil structure, fabricated on a multi-layer printed circuit board, is used to build the optimized coil. A prototype probe with the optimized coil and 32 giant magnetoresistive (GMR) sensors is built and tested on a two-layer riveted aluminum sample. Experimental results show that the optimized probe has better defect detection capability compared with a conventional non-optimized coil.