RESUMO
BACKGROUND: It has been shown that functional status of dendritic cells (DCs) in diabetic patients with unstable angina pectoris (UAP) are more mature and activated than diabetic patients without coronary artery disease (CAD) and none diabetic patients with UAP. Accordingly we aimed to assess the activation of DCs in patients with CAD with/and without Diabetes Mellitus (DM) and compare to those in subjects with normal coronary arteries (NCA). MATERIALS AND METHODS: Twenty three patients with severe CAD who were scheduled to coronary artery by-pass grafting surgery and 6 patients with angiographycally NCAs were included in the study. Activation of peripheral blood DCs have been analyzed by flow cytometric measures of CD86 activation. RESULTS: In patients with CAD and without DM, DC activation significantly increased after stimulation of oxidesized LDL (135⯱â¯121 vs 248⯱â¯197 pâ¯=â¯0.024). However this activation didn't significantly increased in patients with CAD and DM (100⯱â¯20 vs 120⯱â¯97, pâ¯=â¯0,54). Patients with NCAs and without DM showed marked activation of CD86 after stimulation with ox-LDL. CONCLUSION: We have documented that DC activation, upon stimulation of ox-LDL has blunted in patients with CAD compared to patients with NCAs. Moreover this defective activation is more pronounced in those with diabetic patients with CAD.
Assuntos
Doença da Artéria Coronariana/complicações , Doença da Artéria Coronariana/imunologia , Células Dendríticas/fisiologia , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/imunologia , Idoso , Angina Instável/sangue , Angina Instável/complicações , Angina Instável/imunologia , Apresentação de Antígeno/fisiologia , Antígeno B7-2/metabolismo , Estudos de Casos e Controles , Doença da Artéria Coronariana/sangue , Células Dendríticas/metabolismo , Células Dendríticas/patologia , Diabetes Mellitus Tipo 2/sangue , Angiopatias Diabéticas/sangue , Angiopatias Diabéticas/imunologia , Feminino , Citometria de Fluxo , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
We have read with great enthusiasm the article recently published by Boles et al. [...].