Integrated Bioinformatics and Machine Learning Analysis Identify ACADL as a Potent Biomarker of Reactive Mesothelial Cells.

Mesothelial cells with reactive hyperplasia are difficult to distinguish from malignant mesothelioma cells on cell morphology. This study aimed to identify and validate potential biomarkers that distinguish mesothelial cells from mesothelioma cells through machine learning combined with immunohistochemistry experiments. This study integrated the gene expression matrix from three Gene Expression Omnibus data sets (GSE2549, GSE12345, and GSE51024) to analyze the differently expressed genes between normal and mesothelioma tissues. Then, three machine learning algorithms, least absolute shrinkage and selection operator, support vector machine-recursive feature elimination, and random forest were used to screen and obtain four shared candidate markers, including ACADL, EMP2, GPD1L, and HMMR. The receiver operating characteristic curve analysis showed that the area under the curve for distinguishing normal from mesothelioma was 0.976, 0.943, 0.962, and 0.956, respectively. The expression and diagnostic performance of these candidate genes were validated in another two independent data sets (GSE42977 and GSE112154), indicating that the performances of ACADL, GPD1L, and HMMR were consistent between the training and validation data sets. Finally, the optimal candidate marker ACADL was verified by immunohistochemistry assay. ACADL was stained strongly in mesothelial cells, especially for reactive hyperplasic mesothelial cells, but was negative in malignant mesothelioma cells. Therefore, ACADL has the potential to be used as a specific marker of reactive hyperplasic mesothelial cells in the differential diagnosis of mesothelioma.

Nostalgia, Ritual Engagement, and Meaning in Life.

Rituals are pervasive and beneficial. Little is known, however, about causes or antecedents of ritual engagement. We hypothesized that nostalgia-a sentimental longing for one's past-promotes ritual engagement, which in turn augments meaning in life. We tested this hypothesis in five methodologically diverse studies. In Study 1 (N = 311), nostalgia was positively associated with ritual engagement. In Study 2 (N = 188), nostalgia promoted ritual engagement, and in Study 3 (N = 296), it did so over engagement in a neutral task. In Study 4 (N = 252), nostalgia predicted later ritual engagement but not vice versa, convergent with Studies 2 and 3. Furthermore, nostalgia prospectively predicted meaning in life through specific ritualistic behaviors during a traditional festival. Finally, in Study 5 (N = 166), experimentally manipulated ritual engagement augmented meaning in life. As hypothesized, nostalgia advances ritual engagement, contributing to a meaningful life.

Nostalgia Promotes Parents' Tradition Transfer to Children by Strengthening Parent-Child Relationship Closeness.

Parental tradition transfer to children is pivotal for their socialization, identity formation, and culture perpetuation. But what motivates parents to transfer traditions to their children? We hypothesized that nostalgia, an emotion strengthening interpersonal bonds, would promote tradition transfer through parent-child relationship closeness. We tested these hypotheses using cross-sectional (Studies 1 and 4), cross-lagged (Study 2 and preregistered Study 5), and experimental (Studies 3 and 6) designs. In Studies 1 to 3, nostalgia was associated with, had lagged effect on, and promoted tradition transfer. In Studies 4-6, parent-child relationship closeness mediated the link between nostalgia and tradition transfer. The findings enrich our understanding of the vertical transmission of knowledge, customs, and values, offering insight into how intergenerational bonds are reinforced and cultural heritage is maintained.