RESUMO
Sturgeons belong to a subclass of fishes that derived from ray-finned fish ancestors preceding the emergence of teleosts. The Russian sturgeon (Acipenser gueldenstaedtii) is a late-maturing fish with the females reaching puberty under aquaculture conditions at 6-10 years of age. Since kisspeptin has been shown to be a key hormone involved in regulation of major reproductive processes of many vertebrate species, this study was conducted to better understand the kisspeptin receptor (KissR) in sturgeon. In this study we have cloned Russian sturgeon KissR1 from brain mRNA and observed the ontogeny of rsKissR1 mRNA expression in ovarian follicles. Multiple sequence alignment of KissR1, KissR4, and their orthologs revealed that the Russian sturgeon (rs) KissR1 sequence shares 64%-77% identity with elephant shark, coelacanth, and gar and 44-58% identity with tetrapod and teleost KissR1 sequences, while KissR4 seemed to share <65% identity to eel KissR2 and ~57% identity to Perciformes and Cypriniformes. Further rsKissR4 showed <97% identity to reed fish KissR4, <63% with Squamata (Reptiles) and gar KissR4. A phylogenetic analysis revealed that rsKissR1 is more closely related to coelacanth and gar KissR1 than teleost, while rsKissR4 was part of the KissR4 clade and shared higher similarity with Actinopterygiian sequences. We have further predicted homology models for both rsKiss receptors and performed in-silico analyses of their binding to a kiss-10 peptide. Both sturgeon and zebrafish Kiss1 and Kiss2 activated rsKissR1 via both PKC/Ca2+ and PKA/cAMP signal-transduction pathways, while rsKissR2 was found to be less effective and was not activated by stKiss peptides. Ovarian rsKissR transcript levels for 10 fishes were determined by real-time PCR and significantly increased concomitantly with oogenesis, where the highest level of expression was evident in black follicles. These data suggest that extra-neuronal expression of the kisspeptin receptor may be involved in sturgeon reproduction in a manner dependent on reproductive development.
Assuntos
Kisspeptinas , Peixe-Zebra , Animais , Feminino , Expressão Gênica , Kisspeptinas/genética , Oogênese/genética , Filogenia , Federação Russa , Maturidade SexualRESUMO
Sturgeons are being used in aquaculture because wild populations are now endangered due to overfishing for caviar. A challenge in working with sturgeon as an aquacultured species is its long and slow reproductive development. Reproduction is a hormonally regulated process that involves hierarchical signaling between the brain, pituitary gland, and gonads. In an effort to better understand the hormonal regulation of sturgeon reproduction, we have cloned the Russian sturgeon (st), Acipenser gueldenstaedtii, luteinizing hormone receptor (stLHR) and follicle stimulating hormone receptor (stFSHR) and measured their expression from previtellogenic to mature ovarian follicles. Sturgeon LHR and FSHR expression was elevated in early-vitellogenic and mature follicles compared with pre-vitellogenic and mid-vitellogenic follicles, and only LHR expression increased during late-vitellogenesis. Recombinant sturgeon FSH and LH both activated sturgeon LHR and FSHR in a cAMP reporter assay. Further molecular characterization of these receptors was accomplished by in silico modeling and cAMP reporter assays using heterologous recombinant gonadotropins from human and piscine species. There was no apparent trend in heterologous LH and/or FSH activation of the sturgeon LHR or FSHR. These data suggest that permissive activation of LHR and FSHR are a consequence of some yet undetermined biological characteristic(s) of different piscine species.
Assuntos
Regulação da Expressão Gênica , Receptores da Gonadotropina/genética , Receptores da Gonadotropina/metabolismo , Sequência de Aminoácidos , Animais , Clonagem Molecular , Feminino , Humanos , Modelos Moleculares , Filogenia , Domínios Proteicos , Receptores do FSH/química , Receptores do FSH/genética , Receptores do FSH/metabolismo , Receptores da Gonadotropina/química , Receptores do LH/química , Receptores do LH/genética , Receptores do LH/metabolismo , Federação RussaRESUMO
In the reproduction process of male and female fish, pituitary derived gonadotropins (GTHs) play a key role. To be able to specifically investigate certain functions of Luteinizing (LH) and Follicle stimulating hormone (FSH) in Russian sturgeon (Acipenser gueldenstaedtii; st), we produced recombinant variants of the hormones using the yeast Pichia pastoris as a protein production system. We accomplished to create in vitro biologically active heterodimeric glycoproteins consisting of two associated α- and ß-subunits in sufficient quantities. Three dimensional modelling of both GTHs was conducted in order to study the differences between the two GTHs. Antibodies were produced against the unique ß-subunit of each of the GTHs, in order to be used for immunohistochemical analysis and to develop an ELISA for blood and pituitary hormone quantification. This detection technique revealed the specific localization of the LH and FSH cells in the sturgeon pituitary and pointed out that both cell types are present in substantially higher numbers in mature males and females, compared to immature fish. With the newly attained option to prevent cross-contamination when investigating on the effects of GTH administration, we compared the steroidogeneic response (estradiol and 11-Keto testosterone (11-KT) in female and males, respectively) of recombinant stLH, stFSH, and carp pituitary extract in male and female sturgeon gonads at different developmental stages. Finally, we injected commercially available gonadotropin releasing hormones analog (GnRH) to mature females, and found a moderate effect on the development of ovarian follicles. Application of only testosterone (T) resulted in a significant increase in circulating levels of 11-KT whereas the combination of GnRH + T did not affect steroid levels at all. The response pattern for estradiol demonstrated a similar situation. FSH levels showed significant increases when GnRH + T was administered, while no changes were present in LH levels.
Assuntos
Peixes/fisiologia , Gonadotropinas Hipofisárias/fisiologia , Esteroides/fisiologia , Animais , Ensaio de Imunoadsorção Enzimática , Estradiol/metabolismo , Feminino , Hormônio Foliculoestimulante/química , Hormônio Foliculoestimulante/farmacologia , Hormônio Liberador de Gonadotropina/análogos & derivados , Hormônio Liberador de Gonadotropina/farmacologia , Gonadotropinas Hipofisárias/química , Hormônio Luteinizante/química , Hormônio Luteinizante/farmacologia , Masculino , Modelos Moleculares , Folículo Ovariano/efeitos dos fármacos , Folículo Ovariano/fisiologia , Hipófise/efeitos dos fármacos , Hipófise/fisiologia , Subunidades Proteicas , Proteínas Recombinantes/química , Proteínas Recombinantes/farmacologia , Testículo/efeitos dos fármacos , Testículo/fisiologia , Testosterona/análogos & derivados , Testosterona/metabolismo , Testosterona/farmacologiaRESUMO
In this study, the involvement of the cytochrome P450 aromatase gene (CYP19) in the gametogenesis of the teleost blue gourami (Trichogaster trichopterus) is described. The blue gourami brain CYP19 (bgCYP19b) and gonadal CYP19 (bgCYP19a) aromatase genes were cloned and their expression analyzed during the different reproductive stages. The cloned cDNAs of the bgCYP19b and bgCYP19a were found to contain segments of 1518 bp (an open reading frame encoding a deduced protein of 506 residues) and 489 bp (encoding a peptide of 163 residues), respectively. Although the mRNA levels of bgCYP19b were very low in females until the vitellogenic phase, they were significantly higher in the final oocyte maturation stage. The aromatase gene mRNA levels in the gonads were significantly lower in females in the high vitellogenic stage, as compared to females during early vitellogenesis or maturation. In males, the mRNA levels of bgCYP19b were significantly lower in juveniles than in mature individuals. However, no significant differences were observed between mature non-reproductive and reproductive males. In addition, there was no significant difference between the expression of bgCYP19a in juvenile and non-nest building mature males, although a significant increase was detected in mature reproductive males. Although CYP19b expression was similar in both sexes, the expression of CYP19a was significantly different between males and females.