Accounting for spatial heterogeneity in the added conservation value of land protection when prioritizing protected areas.

To combat biodiversity loss, there is increasing interest in safeguarding habitat by expanding protected areas. Given limited resources in conservation, organizations must invest in places that will add the greatest amount of value in species protection. To determine the added conservation value of protection, one needs to consider the level of human disturbance in areas that would result if they were left unprotected. In recent years, data resources have become available that reveal the spatial heterogeneity in human disturbance over large spatial extents worldwide. We investigated how accounting for heterogeneity in future disturbance in unprotected areas affects prioritization of protected areas by determining the added value offered by protection of different areas. We applied a complementarity-based framework for protected area prioritization to select protected areas in the coterminous United States under different assumptions about the heterogeneity of future disturbance in unprotected areas. Prioritizing protected areas while incorrectly assuming spatially homogeneous disturbance in unprotected areas, a common assumption, led to a loss of 76% of possible conservation gain for a given budget. The conservation return on investment from protecting candidate areas was positively correlated (0.44) to future human disturbance in that area if it was left unprotected. Our results show that the ability to identify cost-effective protected area networks depends on how one accounts for the ecological contribution of private lands that remain unprotected.

Existe un creciente interés por salvaguardar los hábitats mediante la expansión de áreas protegidas para combatir la pérdida de la biodiversidad. Debido a los recursos limitados para la conservación, las organizaciones deben invertir en localidades que adicionarán la mayor cantidad de valor a la protección de las especies. Para determinar el valor de conservación adicionado por la protección se necesita considerar el nivel de perturbación humana en las áreas que ocurriría si se les dejara desprotegidas. En años recientes, han quedado disponibles recursos informativos que revelan la heterogeneidad espacial en la perturbación humana a lo largo de grandes extensiones espaciales a nivel mundial. Investigamos cómo considerar esta heterogeneidad en las futuras perturbaciones de las áreas desprotegidas afecta la priorización de las áreas protegidas mediante la determinación del valor adicionado que ofrece la protección de diferentes áreas. Aplicamos un marco de trabajo basado en la complementariedad para la priorización de áreas protegidas para seleccionar estas áreas en los estados colindantes de los Estados Unidos bajo diferentes suposiciones sobre la heterogeneidad de las perturbaciones futuras en las áreas desprotegidas. La priorización de las áreas protegidas mientras se asumía incorrectamente la perturbación espacial homogénea en las áreas desprotegidas, una suposición común, resultó en una pérdida del 76% de la posible ganancia de conservación para un presupuesto dado. El rendimiento de la conservación en la inversión a partir de la protección de las áreas candidatas estuvo correlacionado positivamente (0.44) con las perturbaciones humanas en el futuro si el área permanece desprotegida. Nuestros resultados muestran que la capacidad de identificar las redes rentables de áreas protegidas depende de cómo se consideran las contribuciones ecológicas de las tierras privadas que permanecen desprotegidas.

Full lifetime perspectives on the costs and benefits of lay-date variation in tree swallows.

Animals must balance various costs and benefits when deciding when to breed. The costs and benefits of breeding at different times have received much attention, but most studies have been limited to investigating short-term season-to-season fitness effects. However, breeding early, versus late, in a season may influence lifetime fitness over many years, trading off in complex ways across the breeder's lifespan. In this study, we examined the complete life histories of 867 female tree swallows (Tachycineta bicolor) breeding in Ithaca, New York, between 2002 and 2016. Earlier breeders outperformed later breeders in short-term measures of reproductive output and offspring quality. Though there were weak indications that females paid long-term future survival costs for breeding early, lifetime fledgling output was markedly higher overall in early-breeding birds. Importantly, older females breeding later in the season did not experience compensating life history advantages that suggested an alternative equal-fitness breeding strategy. Rather, most or all of the swallows appear to be breeding as early as they can, and differences in lay dates appear to be determined primarily by differences in individual quality or condition. Lay date had a significant repeatability across breeding attempts by the same female, and the first lay date of females fledged in our population was strongly influenced by the first lay date of their mothers, indicating the potential for ongoing selection on lay date. By examining performance over the entire lifespan of a large number of individuals, we were able to clarify the relationship between timing of breeding and fitness and gain new insight into the sources of variability in this important life history trait.

Author Correction: Alpha-oxoglutarate inhibits the proliferation of immortalized normal bladder epithelial cells via an epigenetic switch involving ARID1A.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.

Alpha-oxoglutarate inhibits the proliferation of immortalized normal bladder epithelial cells via an epigenetic switch involving ARID1A.

Interstitial cystitis (IC) is a chronic urinary tract disease that is characterized by unpleasant sensations, such as persistent pelvic pain, in the absence of infection or other identifiable causes. We previously performed comprehensive metabolomics profiling of urine samples from IC patients using nuclear magnetic resonance and gas-chromatography/mass spectrometry and found that urinary α-oxoglutarate (α-OG), was significantly elevated. α-OG, a tricarboxylic acid (TCA) cycle intermediate, reportedly functions to suppress the proliferation of immortalized normal human bladder epithelial cells. Here, we identified AT-rich interactive domain 1 A (ARID1A), a key chromatin remodeler, as being hypomethylated and upregulated by α-OG treatment. This was done through EPIC DNA methylation profiling and subsequent biochemical approaches, including quantitative RT-PCR and western blot analyses. Furthermore, we found that α-OG almost completely suppresses ten-eleven translocation (TET) activity, but does not affect DNA methyltransferase (DNMT) activity. Altogether, our studies reveal the potential role of α-OG in epigenetic remodeling through its effects on ARID1A and TET expression in the bladder. This may provide a new possible therapeutic strategy in treating IC.

Novel herbal medicine LA16001 ameliorates cisplatin-induced anorexia.

Chemotherapy frequently causes anorexia in cancer patients, which has been associated with poor disease prognosis. Several therapeutic strategies for the treatment of chemotherapy­induced anorexia are available; however, their adverse effects limit their clinical use. Herbal medicines have a long history of use for the treatment of various diseases, including cancer, and recent research has demonstrated their safety and efficacy. In the present study, combinations of herbal medicines were designed based on traditional Korean medicine, and their effects were investigated on chemotherapy­induced anorexia. Herbal mixtures were extracted, composed of Atractylodes japonica, Angelica gigas, Astragalus membranaceus, Lonicera japonica Thunb., Taraxacum platycarpum H. Dahlstedt and Prunella vulgaris var. asiatica (Nakai) Hara. The mixtures were termed LCBP­Anocure­16001­3 (LA16001, LA16002, LA16003). A cisplatin­induced anorexic mouse model was used to evaluate the putative effects of the extracts on chemotherapy­induced anorexia. Treatment with LA16001 was revealed to prevent body weight loss, and all three extracts were demonstrated to improve food intake. When the molecular mechanisms underlying the orexigenic effects of LA16001 were investigated, altered expression levels of ghrelin, leptin and interleukin­6 were revealed. Furthermore, LA16001 was reported to induce phosphorylation of Janus kinase 1 and signal transducer and activator of transcription 3. In addition, LA16001 administration increased the number of white blood cells and neutrophils. These results suggested that the herbal formula LA16001 may be able to prevent chemotherapy­induced anorexia and may have potential as a novel therapeutic strategy for the adjuvant treatment of patients with cancer.