Flavor assessment of a lactic fermented vinegar described in Japanese books from the Edo period (1603-1867).

Aims: Rice vinegar is a traditional fermented seasoning in Japan, and its production remained unchanged for over 800 years until the Edo period. However, based on the available information regarding rice vinegar production methods from this period and the results of reproduction experiments, we speculated that unlike the modern-day acetic fermented vinegar, rice vinegar produced during the Edo period was lactic fermented. Main methods: To verify this assumption, we analyzed the flavor components of Honcho, a lactic fermented product prepared using a method described in books, including "Honchoshokkan" from the Edo period, by capillary electrophoresis/time-of-flight mass spectrometry, high-performance liquid chromatography, gas chromatography mass spectrometry, and taste sensor analysis. Sensory evaluation was also conducted to assess validation as a seasoning. Results: Honcho contains 2 % lactic acid, which gives it its acidity, and small amounts of other nonvolatile acids, but significantly lower levels of acetic acid (0.188 ± 0.015 g/100 mL, p < 0.01). It contains more than double the free amino acids of Kurozu, a modern rice vinegar, and more glutamic acid. Boiling to remove ethanol from yeast fermentation concentrated the free amino acids 1.5 times. Sensor taste analysis showed Honcho had weaker acidity but stronger umami taste than commercial rice vinegar. The volatile compounds related to acetic acid fermentation were significantly different between Honcho and Kurozu. Boiling increased Honcho's acidity, mainly through non-volatile acids. Significance: These findings provide evidence to indicate that Honcho was an acidic seasoning for heat-cooking, which is uncommon in Japanese cuisine today and is mentioned in Edo period books. This seasoning contains many amino acids, implying that it adds umami flavor, not only the sourness of modern vinegar.

d-Arabitol production by a high arabitol-producing yeast, Zygosaccharomyces sp. Gz-5 isolated from miso.

d-Arabitol, an alternative sweetener to sugar, has low calorie content, high sweetness, low glycemic index, and insulin resistance-improving ability. In this study, d-arabitol-producing yeast strains were isolated from various commercial types of miso, and strain Gz-5 was selected among these strains. Phylogenetic tree analysis of the internal transcribed spacer sequence revealed that strain Gz-5 was distinct from Zygosaccharomyces rouxii, a major fermenting yeast of miso. The strain, identified as Zygosaccharomyces sp. Gz-5, grew better than other Z. rouxii in 15 g/L NaCl and produced 114 g/L d-arabitol from 295 g/L glucose in a batch culture for 8 days (0.386 g/g-consumed glucose). In a fed-batch culture, the yeast produced 133 g/L d-arabitol for 14 days, and the total d-arabitol amount increased by 1.75-fold. These results indicated that Zygosaccharomyces sp. Gz-5, a non-genetically modified strain, has excellent potential for the industrial production of d-arabitol.

Nonlinear feedforward enabling quantum computation.

Measurement-based quantum computation with optical time-domain multiplexing is a promising method to realize a quantum computer from the viewpoint of scalability. Fault tolerance and universality are also realizable by preparing appropriate resource quantum states and electro-optical feedforward that is altered based on measurement results. While linear feedforward has been realized and become a common experimental technique, nonlinear feedforward was unrealized until now. In this paper, we demonstrate that a fast and flexible nonlinear feedforward realizes the essential measurement required for fault-tolerant and universal quantum computation. Using non-Gaussian ancillary states, we observed 10% reduction of the measurement excess noise relative to classical vacuum ancilla.

Isolation of Zygosaccharomyces siamensis kiy1 as a novel arabitol-producing yeast and its arabitol production.

Arabitol is gaining attention in the food industry as an alternative sweetener owing to its low-caloric and non-cariogenic characteristics. The yeast strain kiy1 was newly isolated from unpasteurized honey for arabitol production. Based on internal transcribed spacer sequence analysis, the isolated strain was identified as Zygosaccharomyces siamensis. In this study, the effects of different substrates and sugar concentrations on arabitol production were investigated. When three types of carbon sources (glycerol, fructose, and glucose) were used, glucose was the most suitable substrate for arabitol production (68.7 g/L). Maximum arabitol production (101.4 g/L) was observed at a glucose concentration of 30%, and the highest arabitol production yield was 0.34 g/g of initial glucose. In the time-course production of sugar alcohols by strain kiy1, glucose was completely consumed for 8 days. The concentration of arabitol exceeded that of glycerol after 3 days, and the final arabitol concentration reached 83.6 g/L after 10 days. The maximum production rate was 16.7 g/L/day. The yeast produced glycerol as an intracellular sugar alcohol in the early stage of culture and switched its metabolism to arabitol production after the middle stage. Z. siamensis kiy1 possessed an NADP+-dependent arabitol dehydrogenase, which indicated that it probably produces arabitol via ribulose from glucose. These results suggest that the novel yeast strain, Z. siamensis kiy1, is promising for arabitol production. The proposed arabitol production approach can contribute toward its production at the industrial scale.

Low-loss polarization control in fiber systems for quantum computation.

Optical quantum information processing requires low loss interference of quantum light. Also, when the interferometer is composed of optical fibers, degradation of interference visibility due to the finite polarization extinction ratio becomes a problem. Here we propose a low loss method to optimize interference visibility by controlling the polarizations to a crosspoint of two circular trajectories on the Poincaré sphere. Our method maximizes visibility with low optical loss by using fiber stretchers as polarization controllers on both paths of the interferometer. We also experimentally demonstrate our method, where the visibility was maintained basically above 99.9% for three hours using fiber stretchers with an optical loss of 0.02 dB (0.5%). Our method makes fiber systems promising for practical fault-tolerant optical quantum computers.

De novo genome assembly and analysis of Zalaria sp. Him3, a novel fructooligosaccharides producing yeast.

BACKGROUND: Zalaria sp. Him3 was reported as a novel fructooligosaccharides (FOS) producing yeast. However, Zalaria spp. have not been widely known and have been erroneously classified as a different black yeast, Aureobasidium pullulans. In this study, de novo genome assembly and analysis of Zalaria sp. Him3 was demonstrated to confirm the existence of a potential enzyme that facilitates FOS production and to compare with the genome of A. pullulans. RESULTS: The genome of Zalaria sp. Him3 was analyzed; the total read bases and total number of reads were 6.38 Gbp and 42,452,134 reads, respectively. The assembled genome sequence was calculated to be 22.38 Mbp, with 207 contigs, N50 of 885,387, L50 of 10, GC content of 53.8%, and 7,496 genes. g2419, g3120, and g3700 among the predicted genes were annotated as cellulase, xylanase, and ß-fructofuranosidase (FFase), respectively. When the read sequences were mapped to A. pullulans EXF-150 genome as a reference, a small amount of reads (3.89%) corresponded to the reference genome. Phylogenetic tree analysis, which was based on the conserved sequence set consisting of 2,362 orthologs in the genome, indicated genetic differences between Zalaria sp. Him3 and Aureobasidium spp. CONCLUSION: The differences between Zalaria and Aureobasidium spp. were evident at the genome level. g3700 identified in the Zalaria sp. Him3 likely does not encode a highly transfructosyl FFase because the motif sequences were unlike those in other FFases involved in FOS production. Therefore, strain Him3 may produce another FFase. Furthermore, several genes with promising functions were identified and might elicit further interest in Zalaria yeast.

Generation of highly pure single-photon state at telecommunication wavelength.

Telecommunication wavelength with well-developed optical communication technologies and low losses in the waveguide are advantageous for quantum applications. However, an experimental generation of non-classical states called non-Gaussian states at the telecommunication wavelength is still underdeveloped. Here, we generate highly-pure-single-photon states, one of the most primitive non-Gaussian states, by using a heralding scheme with an optical parametric oscillator and a superconducting nano-strip photon detector. The Wigner negativity, the indicator of non-classicality, of the generated single photon state is -0.228 ± 0.004, corresponded to 85.1 ± 0.7% of single photon and the best record of the minimum value at all wavelengths. The quantum-optics-technology we establish can be easily applied to the generation of various types of quantum states, opening up the possibility of continuous-variable-quantum-information processing at the telecommunication wavelength.

Quantum arbitrary waveform generator.

Controlling the temporal waveform of light is the key to a versatile light source in classical and quantum electronics. Although pulse shaping of classical light is mature and has been used in various fields, more advanced applications would be realized by a light source that generates arbitrary quantum light with arbitrary temporal waveforms. We call such a device a quantum arbitrary waveform generator (Q-AWG). The Q-AWG must be able to handle various quantum states of light, which are fragile. Thus, the Q-AWG requires a radically different methodology from classical pulse shaping. Here, we invent an architecture of Q-AWGs that can operate semi-deterministically at a repetition rate over gigahertz in principle. We demonstrate its core technology via generating highly nonclassical states with temporal waveforms that have never been realized before. This result would lead to powerful quantum technologies based on Q-AWGs such as practical optical quantum computing.

Efficient Backcasting Search for Optical Quantum State Synthesis.

Non-Gaussian states are essential for many optical quantum technologies. The so-called optical quantum state synthesizer (OQSS), consisting of Gaussian input states, linear optics, and photon-number resolving detectors, is a promising method for non-Gaussian state preparation. However, an inevitable and crucial problem is the complexity of the numerical simulation of the state preparation on a classical computer. This problem makes it very challenging to generate important non-Gaussian states required for advanced quantum information processing. Thus, an efficient method to design OQSS circuits is highly desirable. To circumvent the problem, we offer a scheme employing a backcasting approach, where the circuit of OQSS is divided into some sublayers, and we simulate the OQSS backwards from final to first layers. Moreover, our results show that the detected photon number by each detector is at most 2, which can significantly reduce the requirements for the photon-number resolving detector. By virtue of the potential for the preparation of a wide variety of non-Gaussian states, the proposed OQSS can be a key ingredient in general optical quantum information processing.

Generation of Schrödinger cat states with Wigner negativity using a continuous-wave low-loss waveguide optical parametric amplifier.

Continuous-wave (CW) squeezed light is used in the generation of various optical quantum states, and thus is a fundamental resource of fault-tolerant universal quantum computation using optical continuous variables. To realize a practical quantum computer, a waveguide optical parametric amplifier (OPA) is an attractive CW squeezed light source in terms of its THz-order bandwidth and suitability for modularization. The usages of a waveguide OPA in quantum applications thus far, however, are limited due to the difficulty of the generation of the squeezed light with a high purity. In this paper, we report the first observation of Wigner negativity of the states generated by a heralding method using a waveguide OPA. We generate Schrödinger cat states at the wavelength of 1545 nm with Wigner negativity using a quasi-single-mode ZnO-doped periodically poled LiNbO3 waveguide module we developed. Wigner negativity is regarded as an important indicator of the usefulness of the quantum states as it is essential in the fault-tolerant universal quantum computation. Our result shows that our waveguide OPA can be used in wide range of quantum applications leading to a THz-clock optical quantum computer.

Decolorization of cationic dyes under alkaline conditions by Iodidimonas sp. Q-1 multicopper oxidase.

Previously, we found that a multicopper oxidase (IOX) produced by Iodidimonas sp. Q-1, an iodide (I-)-oxidizing marine bacterium, exhibited significant decolorization activity toward various anionic dyes. In this study, the potential capacity of IOX for decolorization of cationic dyes such as malachite green (MG), crystal violet (CV), and methylene blue (MB) was determined. Decolorization of the dyes by IOX exhibited significant pH dependence, and effective decolorization was observed under alkaline conditions. At an optimum pH of 9.5, IOX decolorized more than 90% of MG, CV, and MB within 30 min, 2 h, and 6 h, respectively. The addition of iodide was indispensable for decolorization, suggesting that this halide ion serves as a redox mediator. Decolorization products of MG showed less toxicity than MG against Escherichia coli cells. These results suggest that this IOX-iodide system can be used for the decolorization and detoxification of cationic dyes under alkaline conditions.

Isolation and identification of Zalaria sp. Him3 as a novel fructooligosaccharides-producing yeast.

AIMS: This study aimed at obtaining a novel fructooligosaccharides (FOS)-producing yeast, which was different from conventional FOS producers, Aureobasidium spp. METHODS AND RESULTS: Strain Him3 was newly isolated from a Japanese dried sweet potato as a FOS producer. The strain exhibited yeast-like cells and melanization on the potato dextrose agar medium, and formed very weak pseudomycelia on the yeast extract polypeptone dextrose agar medium. Based on the internal transcribed spacer (ITS) region of ribosomal DNA and a partial ß-tubulin gene sequences, the strain Him3 was identified as Zalaria sp. The ß-fructofuranosidase (FFase) produced by strain Him3 was localized on the cell surface (CS-FFase) as well as in the culture broth (EC-FFase). The FOS production yields by CS-FFase and EC-FFase from 50% sucrose were 63.8% and 64.6%, respectively, to consumed sucrose after the reaction for 72 h. CONCLUSIONS: We successfully isolated a novel black yeast, Zalaria sp. Him3, with effective capacity for FOS production. Phylogenetic analysis revealed that strain Him3 was distantly related with the conventional FOS producers, Aureobasidium spp. SIGNIFICANCE AND IMPACT OF THE STUDY: Since FFase of strain Him3 demonstrated high production yields of FOS, it could be applied to novel industrial production of FOS, which is different from conventional methods.

Lenvatinib-induced tumor lysis syndrome in a patient with advanced hepatocellular carcinoma: a case report.

Tumor lysis syndrome (TLS) is an oncologic emergency caused by release of intracellular tumor components due to massive tumor lysis and is rare in patients with hepatocellular carcinoma (HCC). We describe a case of TLS with rupture of HCC induced by lenvatinib in a patient with advanced HCC. A 72-year-old man who presented with right upper abdominal pain was diagnosed as having advanced HCC with a high tumor burden by contrast-enhanced computed tomography and percutaneous hepatic tumor biopsy. He was started on lenvatinib 12 mg once daily when his tumor progressed despite one-shot hepatic arterial infusion chemotherapy. On day 2 of treatment with lenvatinib, he developed severe upper abdominal pain and was diagnosed as having TLS with HCC rupture by laboratory tests and contrast-enhanced computed tomography. Urgent treatment with transarterial embolization, hemodialysis, and blood transfusion therapy was successful. The patient was then restarted on oral lenvatinib at a reduced dose without recurrence of TLS. TLS is a rare potential complication of lenvatinib in patients with advanced HCC and a high tumor burden.

Identification and characterization of proteinase B as an unstable factor for neutral lactase in the enzyme preparation from Kluyveromyces lactis.

The stability of the commercial lactase enzyme is important for the dairy industry. A destabilizing factor for neutral lactase in the enzyme preparation from Kluyveromyces lactis was investigated. We found that lactase had lower thermal stability when fragmented bands of lactase were confirmed on SDS-PAGE. After the destabilizing factor of lactase was purified, that was identified by BLAST search as a hypothetical protein in K. lactis similar to proteinase B (PRB) of Saccharomyces cerevisiae. The molecular mass of protease was estimated to be approximately 30 kDa with SDS-PAGE. The purified protease exhibited activity toward lactase and FITC-casein but not toward bovine serum albumin or milk casein. The optimal pH and temperature of the protease were 8.0 and 40 °C, respectively. The protease activity was strongly inhibited by Fe2+, Cu2+, and a serine protease inhibitor, but activated by Ca2+. Based on these properties, the protease was identified as PRB. Lactase fragmentation was accelerated by the addition of purified PRB to the lactase preparation and was suppressed by protease inhibitors. Thus, this is the first report to identify and characterize PRB as the unstable factor of neutral lactase in the K. lactis preparation.

Phase Locking between Two All-Optical Quantum Memories.

Optical approaches to quantum computation require the creation of multimode photonic quantum states in a controlled fashion. Here we experimentally demonstrate phase locking of two all-optical quantum memories, based on a concatenated cavity system with phase reference beams, for the time-controlled release of two-mode entangled single-photon states. The release time for each mode can be independently determined. The generated states are characterized by two-mode optical homodyne tomography. Entanglement and nonclassicality are preserved for release-time differences up to 400 ns, confirmed by logarithmic negativities and Wigner-function negativities, respectively.

Generation of time-domain-multiplexed two-dimensional cluster state.

Entanglement is the key resource for measurement-based quantum computing. It is stored in quantum states known as cluster states, which are prepared offline and enable quantum computing by means of purely local measurements. Universal quantum computing requires cluster states that are both large and possess (at least) a two-dimensional topology. Continuous-variable cluster states-based on bosonic modes rather than qubits-have previously been generated on a scale exceeding one million modes, but only in one dimension. Here, we report generation of a large-scale two-dimensional continuous-variable cluster state. Its structure consists of a 5- by 1240-site square lattice that was tailored to our highly scalable time-multiplexed experimental platform. It is compatible with Bosonic error-correcting codes that, with higher squeezing, enable fault-tolerant quantum computation.

All-Optical Storage of Phase-Sensitive Quantum States of Light.

We experimentally demonstrate storage and on-demand release of phase-sensitive, photon-number superposition states of the form α|0⟩+ße^{iθ}|1⟩ for an optical quantized oscillator mode. For this purpose, we newly developed a phase-probing mechanism compatible with a storage system composed of two concatenated optical cavities, which was previously employed for storage of phase-insensitive single-photon states [Phys. Rev. X 3, 041028 (2013)PRXHAE2160-330810.1103/PhysRevX.3.041028]. This is the first demonstration of all-optically storing highly nonclassical and phase-sensitive quantum states of light. The strong nonclassicality of the states after storage becomes manifest as a negative region in the corresponding Wigner function shifted away from the origin in phase space. This negativity is otherwise, without the phase information of the memory system, unobtainable. While our scheme includes the possibility of optical storage, on-demand release and synchronization of arbitrary single-rail qubit states, it is not limited to such states. In fact, our technique is extendible to more general phase-sensitive states such as multiphoton superposition or entangled states, and thus it represents a significant step toward advanced optical quantum information processing, where highly nonclassical states are utilized as resources.

Simple method for analyzing the purity of protease-containing samples by acid-treatment SDS-PAGE.

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a widely used technique to analyze the purity of a protein. However, it is necessary to denature (via boiling) the samples before subjecting them to electrophoresis. In the case of protease-containing samples, autolysis of the protease can occur, affecting the accuracy of results. In this study, we investigated the methods for analyzing the purity of Dispase I, a thermolysin-like neutral protease. When we analyzed D protease, a neutral metalloprotease component of Dispase I and highly purified Dispase I using the conventional SDS-PAGE method, a large number of bands were detected in both cases. These bands (putative D protease fragments) were assumed to result from autolysis. To inactivate D protease (optimal pH 7-8), 0.05 M sulfuric acid was utilized (pH 0.7-2.5). Using a conventional sample preparation solution, acid-treated Dispase I samples (without boiling) were made, and SDS-PAGE (15% w/v gel) was carried out. Our findings show that autolysis was inhibited under strong acidic conditions, and protein denaturation was achieved by treatment with sulfuric acid and SDS without boiling. Using this modified SDS-PAGE method, the purities of Dispase I and the purified enzyme were determined to be approximately 80% and 98%, respectively. Furthermore, we demonstrated that this method can be applied for the analysis of other samples including non-acidic proteases (e.g., thermolysin, subtilisin, and trypsin) and protease-contaminated samples (a mixed solution of albumin and D protease).

Generation of a Cat State in an Optical Sideband.

We propose a method to subtract a photon from a double sideband mode of continuous-wave light. The central idea is to use phase modulation as a frequency sideband beam splitter in the heralding photon subtraction scheme, where a small portion of the sideband mode is down-converted to 0 Hz to provide a trigger photon. An optical cat state is created by applying the proposed method to a squeezed state at 500 MHz sideband, which is generated by an optical parametric oscillator. The Wigner function of the cat state reconstructed from a direct homodyne measurement of the 500 MHz sideband modes shows the negativity of W(0,0)=-0.088±0.001 without any loss corrections.

Preoperative diagnosis of the sclerosed hemangioma of the liver using multimodality imaging findings: A case report.

A sclerosed hemangioma of the liver is a rare benign lesion characterized by fibrosis and hyalinization of a hepatic cavernous hemangioma as a result of degeneration. This condition has been difficult to correctly diagnose with imaging. Our patient was a 57-year-old man whose computed tomography (CT) scan showed a mass of 45 mm in diameter in the lateral segment. On dynamic contrast-enhanced CT, the lesion was found to comprise peripheral, gradual, and heterogeneous enhanced areas with a central nonenhanced area; malignancy was suspected. On magnetic resonance imaging, the peripheral area showed slight hperintensity on T2-weighted image, and showed a similar intensity on T1- and diffusion-weighted images as compared to the background liver and gradual enhancement, and the presence of abundant fibrous tissue was suspected. Conversely, the central area showed remarkable hyperintensity on T2-weighted images and no enhancement, and degeneration or hyalinization was suspected. The mass showed no uptake of fluorine-18 fludeoxyglucose (FDG). Some imaging findings suspected a benign tumor, and sclerosed hemangioma with abundant fibrosis and hyalinization was pathologically confirmed. Herein, we report a case of sclerosed hemangioma focusing on possible preoperative diagnosis using a combination of multimodality imaging findings-diffusion-weighted imaging and FDG-positron emission tomography imaging.