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FEMS Microbiol Lett ; 276(1): 19-25, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17937661

RESUMO

In order to isolate and identify the developmentally regulated genes during fruiting body development, cDNA libraries were constructed from eight developmental stages of the Oyster mushroom, Pleurotus ostreatus. From these libraries, 11 761 expressed sequence tags (PoESTs) were generated. Of these, 4060 different genes (PoUnigenes) were identified, representing 34.5% of the entire genome. Redundancy analysis of ESTs during the developmental stages identified eight, 13 and two genes that were specifically expressed in mycelia, fruiting body and basidiospore, respectively. RT-PCR was used to confirm the specific expression of nine genes which showed specific redundancy in fruiting body stages, four genes of which were expressed specifically in fruiting body stages as expected in redundancy analysis, and other genes were expressed abundantly in fruiting body stages. The EST database of P. ostreatus generated during this study provides a genetic and biochemical basis for future studies of the developmental stages of basidiomycetes.


Assuntos
Etiquetas de Sequências Expressas , Perfilação da Expressão Gênica , Regulação Fúngica da Expressão Gênica , Pleurotus/crescimento & desenvolvimento , Pleurotus/genética , DNA Fúngico/genética , Carpóforos/genética , Carpóforos/crescimento & desenvolvimento , Biblioteca Gênica , Genes Fúngicos , Micélio/genética , Micélio/crescimento & desenvolvimento , RNA Fúngico/biossíntese , RNA Mensageiro/biossíntese , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Esporos Fúngicos/genética , Esporos Fúngicos/crescimento & desenvolvimento
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