RESUMO
OBJECTIVES: This study assessed the effect of 2 types of hydroxyethyl starches (HES) on renal integrity and blood transfusion in cardiac surgery patients. DESIGN: Retrospective investigation. SETTING: Patients from a single tertiary medical center. PARTICIPANTS: Inclusion criteria included coronary artery bypass graft (CABG) and/or valve surgery that included cardiopulmonary bypass with aortic cross-clamping. INTERVENTIONS: Intraoperative HES and blood product administration. MEASUREMENTS AND MAIN RESULTS: The study comprised 1,265 patients who met inclusion criteria. Of these patients, 70% received HES, and of these, 47% received<1,000 mL and 53% received≥1,000 mL. There was no difference in the development of acute kidney injury between the 2 groups. A parsimonious propensity model for colloids showed that combined CABG and valve surgery were less likely to be associated with HES administration than was CABG alone (OR 0.68, confidence interval [CI] 0.46-0.97; p = 0.04). Intra-aortic balloon pump use was less likely to be associated with HES administration (OR 0.57, CI 0.38-0.86; p = 0.007). Patients with chronic kidney disease, stages 3 to 5, were less likely to receive HES, with an OR of 0.56 (CI 0.38-0.84; p = 0.004); 0.51 (CI 0.20-1.33; p = 0.170); and 0.23 (CI 0.12-0.44; p<0.0001), respectively, for each stage. No difference was noted in red blood cell transfusion. However, fresh frozen plasma, cryoprecipitate, and platelet transfusions were significantly higher with larger volumes of HES, with an OR of 2.03 (CI 1.64-2.52; p<0.001); 1.60 (CI 1.30-1.97; p<0.000); and 1.62 (CI 1.21-2.15; p = 0.006), respectively. No differences in surgical mortality were found between the colloid and noncolloid groups. CONCLUSIONS: This study showed no association of postoperative acute kidney injury and red blood cell transfusion between the colloid and noncolloid groups. Although the complication rate was higher with HES administration, there was no difference in surgery mortality between the 2 groups.
Assuntos
Injúria Renal Aguda/terapia , Procedimentos Cirúrgicos Cardíacos/efeitos adversos , Transfusão de Eritrócitos/tendências , Derivados de Hidroxietil Amido/administração & dosagem , Cuidados Intraoperatórios/tendências , Complicações Pós-Operatórias/terapia , Injúria Renal Aguda/diagnóstico , Injúria Renal Aguda/etiologia , Idoso , Coloides/administração & dosagem , Transfusão de Eritrócitos/métodos , Feminino , Humanos , Cuidados Intraoperatórios/métodos , Masculino , Pessoa de Meia-Idade , Substitutos do Plasma/administração & dosagem , Complicações Pós-Operatórias/diagnóstico , Complicações Pós-Operatórias/etiologia , Estudos RetrospectivosRESUMO
BACKGROUND: Rigid sternal fixation (RSF) has been shown to reduce sternal wound complications in high-risk patients. However, the higher initial cost continues to deter its use. This study evaluates the cost of caring for high-risk sternotomy patients who underwent RSF compared with those who underwent sternal closure with a modified wire technique (MWT). METHODS: A retrospective single institution review of high-risk patients who underwent MWT (n = 45) and RSF (n = 30) for primary sternal closure from 2006 to 2009 was conducted. Total hospital cost, revenue, and net cost associated with surgery and subsequent care were analyzed. RESULTS: Overall rates of wound dehiscence and wound infections (superficial and deep) were higher in MWT patients (n = 14, 13, and 7, respectively) than RSF patients (n = 3, 2, and 0, respectively; P < 0.05). Modified wire technique patients also required more operations (mean ± SEM: 0.4 ± 0.1 vs 0.1 ± 0.1; P = 0.045), and had longer follow-up time (55.0 ± 9.1 vs 13.4 ± 10.5 days; P = 0.004). Overall, the hospital suffered a greater loss caring for MWT patients (US $18,903 ± 2,160) than RSF patients (US $8,935 ± 2,647). Modified wire technique patients who developed a complication had higher costs associated with their operative hospitalization, outpatient care, and home health than RSF patients (total net loss: US $41,436 ± 7327 vs US $10,612 ± 4,258; P = 0.034). CONCLUSIONS: In high-risk patients, RSF is associated with lower rates of infections, including the "never event" mediastinitis, compared with MWT. Moreover, despite the initial higher cost, RSF affords an overall lower cost of care compared with MWT in patients at high-risk for developing sternal complications.
Assuntos
Fios Ortopédicos , Esternotomia , Técnicas de Fechamento de Ferimentos/economia , Custos e Análise de Custo , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/economia , Complicações Pós-Operatórias/prevenção & controle , Estudos Retrospectivos , Resultado do TratamentoRESUMO
BACKGROUND: Atrial fibrillation represents the most common arrhythmia leading to increased morbidity and mortality, yet, current treatment strategies have proven inadequate. Conventional treatment with antiarrhythmic drugs carries a high risk for proarrhythmias. The soluble epoxide hydrolase enzyme catalyzes the hydrolysis of anti-inflammatory epoxy fatty acids, including epoxyeicosatrienoic acids from arachidonic acid to the corresponding proinflammatory diols. Therefore, the goal of the study is to directly test the hypotheses that inhibition of the soluble epoxide hydrolase enzyme can result in an increase in the levels of epoxyeicosatrienoic acids, leading to the attenuation of atrial structural and electric remodeling and the prevention of atrial fibrillation. METHODS AND RESULTS: For the first time, we report findings that inhibition of soluble epoxide hydrolase reduces inflammation, oxidative stress, atrial structural, and electric remodeling. Treatment with soluble epoxide hydrolase inhibitor significantly reduces the activation of key inflammatory signaling molecules, including the transcription factor nuclear factor κ-light-chain-enhancer, mitogen-activated protein kinase, and transforming growth factor-ß. CONCLUSIONS: This study provides insights into the underlying molecular mechanisms leading to atrial fibrillation by inflammation and represents a paradigm shift from conventional antiarrhythmic drugs, which block downstream events to a novel upstream therapeutic target by counteracting the inflammatory processes in atrial fibrillation.
Assuntos
Antiarrítmicos/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Fibrilação Atrial/metabolismo , Remodelamento Atrial/fisiologia , Inibidores Enzimáticos/uso terapêutico , Átrios do Coração/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Animais , Fibrilação Atrial/tratamento farmacológico , Fibrilação Atrial/fisiopatologia , Remodelamento Atrial/efeitos dos fármacos , Modelos Animais de Doenças , Átrios do Coração/efeitos dos fármacos , Átrios do Coração/fisiopatologia , Masculino , Camundongos , Camundongos Endogâmicos C57BLRESUMO
BACKGROUND: More than 40% of patients undergoing coronary artery bypass grafting (CABG) have diabetes. However, it is unknown how insulin treatment status influences cardiac surgical outcomes among patients with diabetes. METHODS: All isolated CABG, CABG plus aortic valve replacement or plus mitral valve repair/replacement procedures performed in 2012 were extracted from the California CABG Outcomes Reporting Program database. Patients were grouped into three categories: (1) no diabetes, (2) non-insulin-treated diabetes (NITDM), and (3) insulin-treated diabetes (ITDM). Demographic and clinical baseline characteristics and observed postoperative major adverse events, including 30-day mortality, stroke, deep sternal wound infection, prolonged ventilation, new dialysis requirement, renal failure, and 30-day readmission were compared. Multivariable logistic regression models were developed for predicting the impact of NITDM and ITDM on postoperative major adverse events. RESULTS: A total of 14,051 patients underwent isolated CABG or CABG plus aortic/mitral valve procedures in California during 2012; 6700 (47.7%) had no diabetes, 5165 (36.8%) had NITDM, and 2183 (15.6%) had ITDM. Compared with the nondiabetic and NITDM groups, the ITDM group was younger, more frequently women and nonwhite, and had a higher prevalence of preoperative comorbidities (all p < 0.05). After adjusting for baseline risk factors and surgery type compared with patients without diabetes, both NITDM and ITDM were associated with significantly increased risk of major adverse events [NITDM: adjusted odds ratio (AOR), 1.15, 95 % confidence interval (CI), 1.04 to 1.26, p = 0.005; ITDM: AOR, 1.49, 95% CI, 1.32 to 1.68, p < 0.0001]. A subgroup comparison indicated a similar gradient of risk for each category of cardiac surgery. CONCLUSIONS: Patients with diabetes undergoing CABG have substantially increased risk of major adverse events. Patients with ITDM represent an especially high-risk group.
Assuntos
Ponte de Artéria Coronária/métodos , Doença da Artéria Coronariana/cirurgia , Diabetes Mellitus Tipo 1/tratamento farmacológico , Diabetes Mellitus Tipo 2/tratamento farmacológico , Hipoglicemiantes/uso terapêutico , Insulina/uso terapêutico , Complicações Pós-Operatórias/epidemiologia , Idoso , California/epidemiologia , Doença da Artéria Coronariana/complicações , Diabetes Mellitus Tipo 1/complicações , Feminino , Mortalidade Hospitalar/tendências , Humanos , Masculino , Pessoa de Meia-Idade , Morbidade/tendências , Fatores de Risco , Taxa de Sobrevida/tendências , Resultado do TratamentoRESUMO
OBJECTIVES: Cardiothoracic surgeons and practitioners of cardiovascular medicine have a long history of humanitarian aid. Although this is worthwhile at multiple levels and occasionally described in some detail, few efforts have a proven algorithm with demonstrable outcomes that suggest effective educational methodology or clinical results approaching accepted standards in developed countries. METHODS: Our report provides a stepwise approach to developing highly successful self-sustainable, replicable, and scalable humanitarian congenital cardiac surgical programs, and provides data to allow insight into the efficacy of our model. RESULTS: This program model has evolved over 25 years, during which it has been replicated several times and scaled throughout a vast and populous country. Since 1989, Russia has undergone considerable social, political, and economic changes. Our program model proved successful throughout this time despite dynamic social, political, and medical landscapes. CONCLUSIONS: The positive results of our program model indicate that these methodologies may be helpful to others attempting to address the worldwide shortage of cardiovascular care and particularly the complex interventions required in the management of congenital cardiovascular disease.
Assuntos
Altruísmo , Cardiologia/organização & administração , Atenção à Saúde/organização & administração , Cardiopatias Congênitas/cirurgia , Missões Médicas/organização & administração , Modelos Organizacionais , Cirurgia Torácica/organização & administração , Cardiologia/educação , Comportamento Cooperativo , Educação Médica , Cardiopatias Congênitas/diagnóstico , Humanos , Cooperação Internacional , Liderança , Desenvolvimento de Programas , Avaliação de Programas e Projetos de Saúde , Qualidade da Assistência à Saúde/organização & administração , Federação Russa , Cirurgia Torácica/educação , Fatores de Tempo , Resultado do Tratamento , Estados Unidos , VoluntáriosRESUMO
BACKGROUND: We evaluated the impact of intraoperative conversion from off-pump coronary artery bypass graft surgery (OPCAB) to conventional coronary artery bypass graft surgery (CABG) with cardiopulmonary bypass (CCB) on rates of postoperative 30-day hospital readmissions. METHODS: Using data from the California CABG outcomes reporting program, postoperative 30-day hospital readmissions were compared among CCB, OPCAB, and intraoperative conversions from OPCAB to CCB (IOC) for isolated CABG operations. A multivariable logistic regression model with inverse propensity for OPCAB weighting was used to compute risk-adjusted readmission rates. General linear models were used to test the differences in propensity-weighted and risk-adjusted readmission rates among CCB, OPCAB, and IOC cases. RESULTS: Among 22,389 isolated CABGs, 5,125 (22.9%) were OPCAB; 595 (11.6%) OPCAB patients had intraoperative conversion from OPCAB to CCB (IOC). The patients who underwent IOC had a higher prevalence of preoperative heart failure, left main coronary artery disease, and 3 or greater diseased coronary vessels compared with OPCAB without conversion. The risk-adjusted readmission model (weighted for OPCAB propensity) showed OPCAB without conversion was not associated with any increase in readmission rates when compared with CCB (adjusted odds ratio [AOR]; 1.02, 95% confidence interval [CI], 0.963 to 1.081) but OPCAB with IOC had a significant effect on readmission (AOR, 1.258; 95% CI, 1.122 to 1.411, p<0.0001). The OPCAB with IOC was also associated with a higher proportion of readmissions due to postoperative infection (19.1% vs 11.9% of readmissions for CCB). CONCLUSIONS: Intraoperative conversions from OPCAB to CCB are associated with a higher rate of postoperative hospital readmission and infection.
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Ponte de Artéria Coronária/métodos , Doença da Artéria Coronariana/cirurgia , Readmissão do Paciente/tendências , Risco Ajustado/métodos , Idoso , California/epidemiologia , Ponte de Artéria Coronária sem Circulação Extracorpórea/métodos , Doença da Artéria Coronariana/mortalidade , Feminino , Seguimentos , Mortalidade Hospitalar/tendências , Humanos , Período Intraoperatório , Masculino , Período Pós-Operatório , Estudos Retrospectivos , Fatores de Tempo , Resultado do TratamentoRESUMO
BACKGROUND: AND OBJECTIVES: The aim of this retrospective investigation was to study the relationships among chronic kidney disease, acute kidney injury (AKI), and potential benefits by post-bypass dexmedetomidine use in patients undergoing cardiac surgery. METHODS: The patient data were reviewed from the institutional Society of Thoracic Surgeons National Adult Cardiac Surgery Database after IRB approval. 1,133 patients were identified and divided into two groups: those who received dexmedetomidine or those who did not during the post-bypass period. The postoperative outcomes include the incidence of AKI, any complication and all cause of mortality. RESULTS: Post-bypass dexmedetomidine use was associated with significantly reduced the incidence of total AKI (26.1% vs. 33.75%; adjusted OR, 0.7033; 95%CI, 0.540 to 0.916; p=0.0089). In addition, post-bypass dexmedetomidine use was more likely to reduce the incidence of AKI in these patients with preoperative normal kidney function (Stage1; 32.8% to 22.8%; p=0.0233) and mild CKD (Stage 2; 32.8% to 24.7; p=0.0003) after cardiac surgery. Post-bypass infusion of dexmedetomidine was associated with significantly reduced incidence of any complication and 30-day mortalities. CONCLUSIONS: Post-bypass dexmedetomidine use is associated with a significant reduction in the incidence of AKI, especially mild AKI in patients with preoperative normal renal function and mild CKD undergoing cardiac surgery.
Assuntos
Injúria Renal Aguda/prevenção & controle , Agonistas de Receptores Adrenérgicos alfa 2/uso terapêutico , Procedimentos Cirúrgicos Cardíacos/efeitos adversos , Ponte Cardiopulmonar/efeitos adversos , Dexmedetomidina/uso terapêutico , Complicações Pós-Operatórias/prevenção & controle , Injúria Renal Aguda/etiologia , Injúria Renal Aguda/fisiopatologia , Idoso , Humanos , Incidência , Rim/efeitos dos fármacos , Rim/fisiopatologia , Masculino , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/fisiopatologia , Período Pós-Operatório , Insuficiência Renal Crônica/complicações , Estudos Retrospectivos , Fatores de RiscoRESUMO
BACKGROUND: Concomitant aortic valve replacement (AVR) and coronary artery bypass graft surgery (CABG) is a common procedure. Whether the extent of coronary artery disease (CAD) influences outcomes of AVR plus CABG is unknown. METHODS: All AVR plus CABG cases from 2008 to 2010 were extracted from the California CABG Outcomes Reporting Program database. Patients with left main coronary artery stenosis greater than 50% or at least three diseased vessels were defined as having extensive CAD, and patients with one or two diseased coronary vessels were defined as having less extensive CAD. Multivariable logistic regression models were developed for predicting major postoperative complications and 30-day mortality. A Cox proportional hazards model was developed to predict the risk of 1-year mortality. RESULTS: Between 2008 and 2010, 6,151 AVR plus CABG were performed in California. Compared with patients with one- or two-vessel CAD, patients with extensive CAD undergoing AVR plus CABG were on average older, more often male, had greater prevalence of multiple comorbidities, and underwent more urgent or emergent operations (all p < 0.05). After adjusting for baseline risk factors, AVR plus CABG with extensive CAD was associated with significantly increased risk of major postoperative complications (adjusted odds ratio, 1.24; 95% confidence interval, 1.10 to 1.40; p = 0.001) but not operative mortality (adjusted odds ratio, 1.00; 95% confidence interval, 0.77 to 1.29; p = 0.978). A Cox proportional hazards model showed that age and other medical comorbidities, but not extensive CAD, were significant risk factors for 1-year mortality. CONCLUSIONS: Compared with AVR plus CABG for one- or two-vessel CAD, AVR plus CABG for left main or three or more vessel CAD had higher observed and risk-adjusted rates of postoperative complications but not operative or 1-year mortality.
Assuntos
Estenose da Valva Aórtica/cirurgia , Ponte de Artéria Coronária/métodos , Estenose Coronária/cirurgia , Implante de Prótese de Valva Cardíaca/métodos , Idoso , Idoso de 80 Anos ou mais , Estenose da Valva Aórtica/complicações , Estenose da Valva Aórtica/mortalidade , California/epidemiologia , Estenose Coronária/complicações , Estenose Coronária/mortalidade , Feminino , Seguimentos , Humanos , Incidência , Masculino , Complicações Pós-Operatórias/epidemiologia , Estudos Retrospectivos , Fatores de Risco , Taxa de Sobrevida/tendências , Resultado do TratamentoRESUMO
BACKGROUND: Barriers to incorporation of simulation in cardiothoracic surgery training include lack of standardized, validated objective assessment tools. Our aim was to measure interrater reliability and internal consistency reliability of a coronary anastomosis assessment tool created by the Joint Council on Thoracic Surgery Education. METHODS: Ten attending surgeons from different cardiothoracic residency programs evaluated nine video recordings of 5 individuals (1 medical student, 1 resident, 1 fellow, 2 attendings) performing coronary anastomoses on two simulation models, including synthetic graft task station (low fidelity) and porcine explant (high fidelity), as well as in the operative setting. All raters, blinded to operator identity, scored 13 assessment items on a 1 to 5 (low to high) scale. Each performance also received an overall pass/fail determination. Interrater reliability and internal consistency were assessed as intraclass correlation coefficients and Cronbach's α, respectively. RESULTS: Both interrater reliability and internal consistency were high for all three models (intraclass correlation coefficients = 0.98, 0.99, and 0.94, and Cronbach's α = 0.99, 0.98, and 0.97 for low fidelity, high fidelity, and operative setting, respectively). Interrater reliability for overall pass/fail determination using κ were 0.54, 0.86, 0.15 for low fidelity, high fidelity, and operative setting, respectively. CONCLUSIONS: Even without instruction on the assessment tool, experienced surgeons achieved high interrater reliability. Future resident training and evaluation may benefit from utilization of this tool for formative feedback in the simulated and operative environments. However, summative assessment in the operative setting will require further standardization and anchoring.
Assuntos
Competência Clínica/normas , Simulação por Computador , Vasos Coronários/cirurgia , Educação de Pós-Graduação em Medicina/normas , Procedimentos Cirúrgicos Torácicos/educação , Adulto , Anastomose Cirúrgica/educação , Educação de Pós-Graduação em Medicina/tendências , Feminino , Humanos , Internato e Residência/normas , Internato e Residência/tendências , Masculino , Pessoa de Meia-Idade , Avaliação das Necessidades , Variações Dependentes do Observador , Reprodutibilidade dos Testes , Sociedades Médicas/normas , Inquéritos e Questionários , Procedimentos Cirúrgicos Torácicos/normas , Estados Unidos , Gravação em VídeoRESUMO
INTRODUCTION: Previous work has demonstrated the efficacy of vacuum-assisted closure (VAC) in the treatment of poststernotomy local wound infections, compared to historical treatment protocol. The negative pressure has been found to protect wounds against contamination, prevent wound fluid retention, increase blood flow, and increase rates of granulation tissue formation. For this study, a retrospective analysis compared patients receiving VAC as definitive treatment versus bridging to delayed flap closure. METHODS: Sixteen patients developed sternal wound infections after cardiac surgeries at the authors' institution from 2006 to 2008. Data was gathered regarding patient comorbidities, treatment method, and outcome. Study objectives included assessment of risk factors that warranted secondary surgicalclosure and examination of long-term followup where VAC was thedefinitive treatment modality. RESULTS: Group A (n = 12) had VAC as the final treatment modality. Group B (n = 4) required myocutaneous flap closure. One patient in Group B passed away prior to flap surgery. Both groups had similar risk factors, except Group B had a higher risk of body mass index (BMI) > 35 that was near statistically significant (P = 0.085; odds ratio = 0.0, 95% CI = [0.0 - 1.21]). Group A required a shorter hospital stay on average. Long-term follow-up showed the majority of Group A had completely healed sternal wounds 2-3 years from initial cardiac surgery. CONCLUSIONS: Vacuum-assisted closure as definitive treatment modality is a successful, first line therapy for local superficial sternal wound infections. When deep infections occur, however, VAC as bridge-to-flap coverage is recommended over attempted secondary healing with VAC. .
RESUMO
Atrial septal aneurysm is an uncommon cardiac anomaly that is usually asymptomatic or occasionally associated with cardioembolic events. We present the unusual impeding effect of an atrial septal aneurysm on venous drainage during cardiopulmonary bypass in a 70-year-old man who underwent aortic valve replacement and coronary artery bypass graft surgery.
Assuntos
Ponte Cardiopulmonar , Aneurisma Cardíaco/complicações , Comunicação Interatrial/complicações , Idoso , Valva Aórtica/cirurgia , Ponte de Artéria Coronária , Ecocardiografia Transesofagiana , Implante de Prótese de Valva Cardíaca , Humanos , MasculinoRESUMO
BACKGROUND: Coronary artery bypass grafting (CABG) is no longer rare for elderly patients. This study evaluates operative mortality and the effects of off-pump CABG (OPCAB) on mortality for elderly Californians between 2003 and 2008. METHODS: All isolated CABGs in California for 2003 to 2008 were classified into cohorts by age: (1) younger than 75, (2) 75 to 84, and (3) 85 or older. Multivariable logistic regression models were developed for operative mortality. Trend analyses for observed and predicted mortality, and observed-to-expected mortality ratios were performed. The "recycled predictions" method was used to assess the effect of OPCAB on operative mortality. RESULTS: Among 101,710 isolated CABGs between 2003 and 2008, 22.0% were in cohort 2 and 2.3% were in cohort 3. Predicted mortality was unchanged for cohorts 2 and 3 (all p > 0.05), but observed-to-expected mortality ratios declined from 0.958 to 0.633 for cohort 2 (p = 0.021) and from 1.027 to 0.965 for cohort 3 (p = 0.168). The proportion of OPCAB for patients aged 75 years or older increased from 25.0% to 29.1% between 2003 and 2008. The adjusted odds ratio for operative mortality for OPCAB in patients aged 75 years or older was 0.752 (95% confidence interval, 0.650 to 0.871; p < 0.001) compared with on-pump CABG for the same age cohort. CONCLUSIONS: In California, overall predicted mortality was unchanged for elderly patients between 2003 and 2008, but operative mortality significantly decreased for patients aged 75 to 84. Improvement for CABG patients aged 85 years or older was insignificant. The increase in the number of OPCAB patients was associated with decreased mortality for elderly patients.
Assuntos
Ponte de Artéria Coronária/mortalidade , Doença da Artéria Coronariana/cirurgia , Sistema de Registros , Idoso , Idoso de 80 Anos ou mais , California/epidemiologia , Estudos de Coortes , Ponte de Artéria Coronária sem Circulação Extracorpórea/mortalidade , Feminino , Humanos , Modelos Logísticos , Masculino , Mortalidade/tendênciasRESUMO
Cardiac progenitor cells (CPCs) are multipotent cells that may offer tremendous potentials for the regeneration of injured myocardium. To expand the limited number of CPCs for effective clinical regeneration of myocardium, it is important to understand their proliferative potentials. Single-cell based assays were utilized to purify c-kit(pos) CPCs from human and mouse hearts. MicroRNA profiling identified eight differentially expressed microRNAs in CPCs from neonatal and adult hearts. Notably, the predicted protein targets were predominantly involved in cellular proliferation-related pathways. To directly test this phenotypic prediction, the developmental variance in the proliferation of CPCs was tested. Ki67 protein expression and DNA kinetics were tested in human and mouse in vivo CPCs, and doubling times were tested in primary culture of mouse CPCs. The human embryonic and mouse neonatal CPCs showed a six-fold increase in Ki67 expressing cells, a two-fold increase in the number of cells in S/G2-M phases of cell cycle, and a seven-fold increase in the doubling time in culture when compared to the corresponding adult CPCs. The over-expression of miR-17-92 increased the proliferation in adult CPCs in vivo by two-fold. In addition, the level of retinoblastoma-like 2 (Rbl2/p130) protein was two-fold higher in adult compared to neonatal-mouse CPCs. In conclusion, we demonstrate a differentially regulated cohort of microRNAs that predicts differences in cellular proliferation in CPCs during postnatal development and target microRNAs that are involved in this transition. Our study provides new insights that may enhance the utilization of adult CPCs for regenerative therapy of the injured myocardium.
Assuntos
Perfilação da Expressão Gênica , MicroRNAs/genética , Mioblastos Cardíacos/metabolismo , Animais , Ciclo Celular/fisiologia , Proliferação de Células , Separação Celular , Células Cultivadas , Análise por Conglomerados , Humanos , Cinética , Camundongos , MicroRNAs/metabolismo , Fenótipo , Proteínas Proto-Oncogênicas c-kit/metabolismo , Proteína p130 Retinoblastoma-Like/metabolismoRESUMO
Complex aortic valve repair after mass lesion resection, in an otherwise normal, thin leafleted valve, is rarely described in the literature. We present a 68-year-old woman who underwent resection of an asymptomatic aortic valve papillary fibroelastoma. Due to extensive involvement of her left coronary cusp, the resection resulted in a significant defect in the leaflet, requiring a complex repair to preserve her otherwise normal aortic valve. We describe the operative findings, repair technique, and associated literature.
Assuntos
Valva Aórtica/cirurgia , Procedimentos Cirúrgicos Cardíacos/métodos , Fibroma/cirurgia , Neoplasias Cardíacas/cirurgia , Doenças das Valvas Cardíacas/cirurgia , Idoso , Valva Aórtica/diagnóstico por imagem , Valva Aórtica/ultraestrutura , Diagnóstico Diferencial , Ecocardiografia Transesofagiana , Feminino , Fibroma/diagnóstico , Neoplasias Cardíacas/diagnóstico , Doenças das Valvas Cardíacas/diagnóstico , Doenças das Valvas Cardíacas/etiologia , Humanos , Achados Incidentais , Tomografia Computadorizada por Raios XRESUMO
The aim of this study was to evaluate whether African American, Hispanic, and Asian patients in California were more likely to undergo coronary artery bypass grafting (CABG) by cardiac surgeons with higher risk-adjusted mortality rates (RAMRs). Clinical data from the California CABG Outcomes Reporting Program were analyzed for all patients who underwent isolated CABG from 2003 to 2006 by surgeons who performed ≥ 10 operations. Surgeons were divided into quintiles on the basis of their RAMRs, with the top-performing surgeons in the first quintile and the lowest performing surgeons in the fifth quintile. There were 72,845 isolated CABG procedures performed by 303 surgeons, including 49,886 in white, 9,380 in Hispanic, 6,867 in Asian, and 2,750 in African American patients. African American and Asian patients underwent CABG by surgeons with higher mean RAMRs (2.90% and 2.99%, respectively) compared with the state average of 2.65% (p <0.001). Compared to white patients, Asian and Hispanic patients were more likely to be treated by surgeons in the lowest quintile (odds ratio [OR] 1.2, 95% confidence interval [CI] 1.11 to 1.3, and OR 1.38, 95% CI 1.30 to 1.48, respectively). African Americans and Hispanics were less likely to be operated on by surgeons in the top quintile compared to white patients (OR 0.80, 95% CI 0.71 to 0.90, and OR 0.81, 95% CI 0.76 to 0.87, respectively). Hispanics were less likely to be treated by surgeons in the top quintile than by surgeons in the lowest quintile (OR 0.65, 95% CI 0.60 to 0.75). In conclusion, racial and ethnic minority patients who undergo isolated CABG in California may be more likely to be operated on by cardiac surgeons with higher RAMRs.
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Ponte de Artéria Coronária/mortalidade , Doença da Artéria Coronariana/etnologia , Doença da Artéria Coronariana/cirurgia , Etnicidade , Médicos/normas , Idoso , California/etnologia , Feminino , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
RATIONALE: Ca(2+)-activated K(+) channels are present in a wide variety of cells. We have previously reported the presence of small conductance Ca(2+)-activated K(+) (SK or K(Ca)) channels in human and mouse cardiac myocytes that contribute functionally toward the shape and duration of cardiac action potentials. Three isoforms of SK channel subunits (SK1, SK2, and SK3) are found to be expressed. Moreover, there is differential expression with more abundant SK channels in the atria and pacemaking tissues compared with the ventricles. SK channels are proposed to be assembled as tetramers similar to other K(+) channels, but the molecular determinants driving their subunit interaction and assembly are not defined in cardiac tissues. OBJECTIVE: To investigate the heteromultimeric formation and the domain necessary for the assembly of 3 SK channel subunits (SK1, SK2, and SK3) into complexes in human and mouse hearts. METHODS AND RESULTS: Here, we provide evidence to support the formation of heteromultimeric complexes among different SK channel subunits in native cardiac tissues. SK1, SK2, and SK3 subunits contain coiled-coil domains (CCDs) in the C termini. In vitro interaction assay supports the direct interaction between CCDs of the channel subunits. Moreover, specific inhibitory peptides derived from CCDs block the Ca(2+)-activated K(+) current in atrial myocytes, which is important for cardiac repolarization. CONCLUSIONS: The data provide evidence for the formation of heteromultimeric complexes among different SK channel subunits in atrial myocytes. Because SK channels are predominantly expressed in atrial myocytes, specific ligands of the different isoforms of SK channel subunits may offer a unique therapeutic opportunity to directly modify atrial cells without interfering with ventricular myocytes.
Assuntos
Potenciais de Ação/fisiologia , Miócitos Cardíacos/fisiologia , Potássio/metabolismo , Canais de Potássio Ativados por Cálcio de Condutância Baixa , Sequência de Aminoácidos , Animais , Células Cultivadas , Átrios do Coração/citologia , Humanos , Camundongos , Dados de Sequência Molecular , Miócitos Cardíacos/citologia , Técnicas de Patch-Clamp , Multimerização Proteica , Estrutura Terciária de Proteína , Canais de Potássio Ativados por Cálcio de Condutância Baixa/química , Canais de Potássio Ativados por Cálcio de Condutância Baixa/genética , Canais de Potássio Ativados por Cálcio de Condutância Baixa/fisiologia , TransfecçãoRESUMO
BACKGROUND: Coronary artery bypass surgery (CABG) is associated with a significant risk of stroke. Some studies suggest that off-pump CABG (OPCAB) may reduce postoperative stroke rate. We performed this study to evaluate the relationship between postoperative stroke and OPCAB compared with conventional on-pump CABG (CCB) in a recent, large cohort of patients. METHODS: Data from the California CABG Outcomes Reporting Program were analyzed in patients who had OPCAB or CCB for isolated CABGs in 2006 to 2007. Two multivariable logistic regression models were developed for the analysis, and the "recycled predictions" method was used to compute risk-adjusted postoperative stroke rates in the two surgical groups. RESULTS: Of 30,426 isolated CABGs, 7,720 (23.7%) were OPCAB. The model developed in the CCB subset indicated that CCB had a lower predicted stroke risk than OPCAB, yet the observed rate of stroke was higher in the CCB subset. The model using both CCB and OPCAB patients revealed that OPCAB was associated with a reduction in postoperative stroke (adjusted odds ratio: 0.76, 95% confidence interval [CI] 0.59 to 0.98). For patients with cardiogenic shock, OPCAB was also associated with a lower risk-adjusted postoperative stroke rate compared with CCB (OPCAB: 3.06%, 95% CI 2.83% to 3.28%; CCB: 4.05%, 95% CI 3.76% to 4.33%, p < 0.001). However, the 793 (11%) OPCAB patients who were converted to CCB intraoperatively had an increased postoperative stroke rate (with conversion: 2.02%, 95% CI 1.04% to 3.00% versus without conversion: 0.96%, 95% CI 0.73% to 1.20%, p < 0.001). CONCLUSIONS: The OPCAB was associated with a significantly lower postoperative stroke rate compared with CCB even for older and higher risk patients. However, intraoperative OPCAB to CCB conversion was associated with the highest postoperative stroke rate.
Assuntos
Ponte de Artéria Coronária sem Circulação Extracorpórea , Acidente Vascular Cerebral/epidemiologia , Acidente Vascular Cerebral/etiologia , Idoso , Ponte de Artéria Coronária/efeitos adversos , Feminino , Humanos , Masculino , Estudos RetrospectivosRESUMO
BACKGROUND: California launched the coronary artery bypass graft surgery (CABG) Outcomes Reporting Program in 2003 for all nonfederal hospitals performing this procedure. The program provides annual public reports of risk-adjusted operative mortality rates by hospital and surgeon. However, the impact of this program on operative mortality and access to surgery for high-risk patients has not been clarified. METHODS: The cohort consisted of all isolated CABG cases from the California CABG Outcomes Reporting Program database for 2003 and 2006. We applied the same multivariable logistic risk adjustment model to each year to compute predicted and risk-adjusted operative mortality for isolated CABG by hospital and surgeons. Changes in surgical volume and observed, predicted, and risk-adjusted operative mortality were compared by quintiles of patients based on the predicted risk and among hospitals and surgeons between 2003 and 2006. RESULTS: Total volume of isolated CABG decreased by 26.5% from 2003 (N=21,276) to 2006 (N=15,647). The reduction in CABG volume between 2003 and 2006 was universal among hospitals and surgeons regardless of their performance status in 2003. The change in patient case mix for a majority of hospitals and surgeons was insignificant, and overall patient risk was stable (statewide predicted operative mortality rate for 2003, 3.06%; 95% confidence interval, 2.98 to 3.13; and for 2006, 3.05%; 95% confidence interval, 2.97 to 3.14). Yet, the statewide observed mortality declined from 2.90% in 2003 to 2.22% in 2006 (p=0.0001). Overall, the empiric odds ratio of operative death for 2006 patients was 24% lower than for 2003 patients. In 2006, patients with the highest predicted operative mortality risk (4th and 5th quintiles) had 35% and 26% lower odds of operative mortality, respectively, when compared with patients from 2003. CONCLUSIONS: Although total CABG volume decreased from 2003 to 2006 by almost 27%, patient case mix for most hospitals and surgeons was unchanged. Despite similar patient characteristics, the operative mortality for patients in the highest risk group was 26% lower in 2006 than in 2003. We found no evidence of decreased access to CABG for high-risk patients in California during the period of public reporting of isolated CABG outcomes.
Assuntos
Ponte de Artéria Coronária/mortalidade , Ponte de Artéria Coronária/estatística & dados numéricos , Idoso , California , Feminino , Acessibilidade aos Serviços de Saúde/estatística & dados numéricos , Mortalidade Hospitalar , Humanos , Masculino , Fatores de Risco , Resultado do TratamentoRESUMO
Percutaneous approaches for treating mitral regurgitation are under investigation, including repair with the MitraClip percutaneous mitral repair system (Evalve, Inc., Menlo Park, CA, USA), which has undergone extensive preclinical and clinical evaluation in the EVEREST I and II trials. The procedure involves the transcatheter placement of one or two MitraClip devices under echocardiographic and fluoroscopic guidance to restore leaflet coaptation. A desirable feature of any percutaneous mitral valve (MV) repair system is that the device should not impede subsequent surgical repair if needed. To date, the majority of reported MV surgeries after MitraClip device implantation have occurred earlier, within one year of treatment. We herein describe four previously unreported cases of successful surgical MV repair up to five years after MitraClip device implantation, demonstrating that late MV repair remains possible, including after implantation of two clips.