RESUMO
A new compound 6-formamide-chetomin (1) together with chetomin (2) was isolated from solid fermentation products of the endophytic fungus Chaetomium sp. M336, which were elucidated by extensive spectroscopic analyses, including 1D and 2D NMR, and HR-ESI-MS experiments. The bioassay result showed that compound 1 had strong antibacterial activity against Escherichia coli, Staphylococcus aureus, Salmonella typhimurium ATCC 6539 and Enterococcus faecalis with minimum inhibitory concentration (MIC) value of 0.78 µg/mL; meanwhile it exhibited strong cytotoxicity with IC50 values of 21.6-27.1 nM against cell lines HeLa, SGC-7901 and A549.
Assuntos
Antibacterianos/farmacologia , Antineoplásicos/farmacologia , Chaetomium/química , Formamidas/farmacologia , Antibacterianos/química , Antineoplásicos/química , Linhagem Celular Tumoral , Dissulfetos/química , Dissulfetos/farmacologia , Endófitos/química , Enterococcus faecalis/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Formamidas/química , Células HeLa , Humanos , Alcaloides Indólicos/química , Alcaloides Indólicos/farmacologia , Testes de Sensibilidade Microbiana , Estrutura Molecular , Salmonella typhimurium/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacosRESUMO
An endophytic fungus, Chaetomium sp. M453, was isolated from Huperzia serrata (Thunb. ex Murray) Trev. and subjected to phytochemical investigation. Three unusual C25 steroids, neocyclocitrinols E-G (1-3), and 3ß-hydroxy-5,9-epoxy-(22E,24R)-ergosta-7,22-dien-6-one (4) together with three known steroids were isolated from solid fermentation products of the fungus, which were elucidated by extensive spectroscopic analyses, including 1D-, 2D-NMR, and HR-ESI-MS experiments. The absolute configuration of 1 was determined by X-ray crystallographic analysis and CD analyses. The acetylcholinesterase inhibitory activities of compounds 1-4 were tested in vitro. Compound 4 showed weak acetylcholinesterase inhibitory activity.
Assuntos
Chaetomium/química , Inibidores da Colinesterase/química , Huperzia/microbiologia , Esteroides/química , Linhagem Celular Tumoral , Inibidores da Colinesterase/isolamento & purificação , Humanos , Estrutura Molecular , Plantas Medicinais/microbiologia , Esteroides/isolamento & purificaçãoRESUMO
The white pine blister rust caused by Cronartium ribicola is a severe disease of Pinus armandii Franch in Sichuan Province, China. A mycoparasite was isolated from aeciospore piles of C. ribicola and identified as Pestalotipsis sp. The chemical constituents were investigated and nine new polyketides, named pestalotic acids A-I (1-9), were isolated from solid fermentation products of Pestalotiopsis sp. cr014. Their structures were elucidated by extensive spectroscopic methods, including 1D- and 2D- NMR, HR-MS experiments. Compounds 2-5 and 7-9 exhibited strong antibacterial activities in vitro against five plant pathogens, with minimum inhibitory concentration values of 0.78-100 µg/mL.