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1.
IEEE J Biomed Health Inform ; 28(2): 730-741, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-37023158

RESUMO

Cell instance segmentation (CIS) via light microscopy and artificial intelligence (AI) is essential to cell and gene therapy-based health care management, which offers the hope of revolutionary health care. An effective CIS method can help clinicians to diagnose neurological disorders and quantify how well these deadly disorders respond to treatment. To address the CIS task challenged by dataset characteristics such as irregular morphology, variation in sizes, cell adhesion, and obscure contours, we propose a novel deep learning model named CellT-Net to actualize effective cell instance segmentation. In particular, the Swin transformer (Swin-T) is used as the basic model to construct the CellT-Net backbone, as the self-attention mechanism can adaptively focus on useful image regions while suppressing irrelevant background information. Moreover, CellT-Net incorporating Swin-T constructs a hierarchical representation and generates multi-scale feature maps that are suitable for detecting and segmenting cells at different scales. A novel composite style named cross-level composition (CLC) is proposed to build composite connections between identical Swin-T models in the CellT-Net backbone and generate more representational features. The earth mover's distance (EMD) loss and binary cross entropy loss are used to train CellT-Net and actualize the precise segmentation of overlapped cells. The LiveCELL and Sartorius datasets are utilized to validate the model effectiveness, and the results demonstrate that CellT-Net can achieve better model performance for dealing with the challenges arising from the characteristics of cell datasets than state-of-the-art models.


Assuntos
Inteligência Artificial , Células Secretoras de Somatostatina , Humanos , Fontes de Energia Elétrica , Entropia , Microscopia , Processamento de Imagem Assistida por Computador
2.
ACS Appl Mater Interfaces ; 15(23): 28175-28183, 2023 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-37276488

RESUMO

Photodetectors (PDs) are critical parts of visible light communication (VLC) systems for achieving efficient photoelectronic conversion and high-fidelity transmission of signals. Antimony sulfide (Sb2S3) as a nontoxic, high optical absorption coefficient, and low-cost semiconductor becomes a promising candidate for applications in VLC systems. Particularly, Sb2S3 PDs were verified to have significantly weak light detection ability in the visible region. However, the response speed of Sb2S3 PDs with existing device structures is still relatively slow. Herein, through optimizing the device structure for the p-i-n type PDs, a p-type Sb2Se3 hole transport layer (HTL) is designed to enhance the built-in electric field and to accelerate the migration of photogenerated carriers for the high responsivity and fast response speed. The optimal thickness of the structure is obtained through the simulation of SCAPS-1D software, and the optimized devices show high-performance parameters, including a responsivity of 0.34 A W-1, a specific detectivity (D*) of 2.20 × 1012 Jones, the -3 dB bandwidth of 440 kHz, high stability, and the value of the Sb2S3 PDs can reach 60% in the range of 360-600 nm, which indicates that the device is very suitable for working in the visible light band. In addition, the resulting Sb2S3 PD is successfully integrated into VLC systems by designing a matched light detection circuit. The results suggest that the Sb2S3 PDs are expected to provide an alternative to future VLC system applications.

3.
Sci Rep ; 11(1): 21900, 2021 11 09.
Artigo em Inglês | MEDLINE | ID: mdl-34754057

RESUMO

Every year, around 28,100 journals publish 2.5 million research publications. Search engines, digital libraries, and citation indexes are used extensively to search these publications. When a user submits a query, it generates a large number of documents among which just a few are relevant. Due to inadequate indexing, the resultant documents are largely unstructured. Publicly known systems mostly index the research papers using keywords rather than using subject hierarchy. Numerous methods reported for performing single-label classification (SLC) or multi-label classification (MLC) are based on content and metadata features. Content-based techniques offer higher outcomes due to the extreme richness of features. But the drawback of content-based techniques is the unavailability of full text in most cases. The use of metadata-based parameters, such as title, keywords, and general terms, acts as an alternative to content. However, existing metadata-based techniques indicate low accuracy due to the use of traditional statistical measures to express textual properties in quantitative form, such as BOW, TF, and TFIDF. These measures may not establish the semantic context of the words. The existing MLC techniques require a specified threshold value to map articles into predetermined categories for which domain knowledge is necessary. The objective of this paper is to get over the limitations of SLC and MLC techniques. To capture the semantic and contextual information of words, the suggested approach leverages the Word2Vec paradigm for textual representation. The suggested model determines threshold values using rigorous data analysis, obviating the necessity for domain expertise. Experimentation is carried out on two datasets from the field of computer science (JUCS and ACM). In comparison to current state-of-the-art methodologies, the proposed model performed well. Experiments yielded average accuracy of 0.86 and 0.84 for JUCS and ACM for SLC, and 0.81 and 0.80 for JUCS and ACM for MLC. On both datasets, the proposed SLC model improved the accuracy up to 4%, while the proposed MLC model increased the accuracy up to 3%.

4.
PeerJ Comput Sci ; 7: e385, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33817031

RESUMO

Frequently used items mining is a significant subject of data mining studies. In the last ten years, due to innovative development, the quantity of data has grown exponentially. For frequent Itemset (FIs) mining applications, it imposes new challenges. Misconceived information may be found in recent algorithms, including both threshold and size based algorithms. Threshold value plays a central role in generating frequent itemsets from the given dataset. Selecting a support threshold value is very complicated for those unaware of the dataset's characteristics. The performance of algorithms for finding FIs without the support threshold is, however, deficient due to heavy computation. Therefore, we have proposed a method to discover FIs without the support threshold, called Top-k frequent itemsets mining (TKFIM). It uses class equivalence and set-theory concepts for mining FIs. The proposed procedure does not miss any FIs; thus, accurate frequent patterns are mined. Furthermore, the results are compared with state-of-the-art techniques such as Top-k miner and Build Once and Mine Once (BOMO). It is found that the proposed TKFIM has outperformed the results of these approaches in terms of execution and performance, achieving 92.70, 35.87, 28.53, and 81.27 percent gain on Top-k miner using Chess, Mushroom, and Connect and T1014D100K datasets, respectively. Similarly, it has achieved a performance gain of 97.14, 100, 78.10, 99.70 percent on BOMO using Chess, Mushroom, Connect, and T1014D100K datasets, respectively. Therefore, it is argued that the proposed procedure may be adopted on a large dataset for better performance.

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