Neuronal transcriptomic responses to Japanese encephalitis virus infection with a special focus on chemokine CXCL11 and pattern recognition receptors RIG-1 and MDA5.

Japanese encephalitis virus (JEV) causes central nervous system neuronal injury and inflammation. A clear understanding of neuronal responses to JEV infection remains elusive. Using the Affymetrix array to investigate the transcriptome of infected SK-N-MC cells, 1316 and 2737 dysregulated genes (≥ 2/-2 fold change, P < 0.05) were found at 48 hours post-infection (hpi) and 60 hpi, respectively. The genes were mainly involved in anti-microbial responses, cell signalling, cellular function and maintenance, and cell death and survival. Among the most highly upregulated genes (≥ 10 folds, P < 0.05) were chemokines CCL5, CXCL11, IL8 and CXCL10. The upregulation and expression of CXCL11 were confirmed by qRT-PCR and immunofluorescence. Pathogen recognition receptors retinoic acid-inducible gene-1 (RIG-1) and melanoma differentiation-associated protein 5 (MDA5) were also upregulated. Our results strongly suggest that neuronal cells play a significant role in immunity against JEV. CXCL11, RIG-1 and MDA5 and other cytokines may be important in neuropathogenesis.

[Effects of typical organic pollutants on marine dioxide carbon system in simulated experiments].

The effects of typical organic pollutants (ethanol, acetone, ON, OP) on pH and marine carbon dioxide system were investigated in laboratory simulated experiments. The results indicated that the growth of biological carbon fixed system was promoted by ethanol and acetone of low concentration (< 0.5 mmol x L(-1)) (t = 7 d). So pH increased while DIC, HCO3(-) and p(CO2) significantly decreased compared to the controls (p <0.01). The concentrations of ethanol and acetone which resulted in the maximum decrease of carbon dioxide parameters were 0.25 mmol x L(-1) and 0.1 mmol x L(-1), respectively. When the infusion of organic pollutants were higher than the "critical concentration", the carbon dioxide parameters increased and pH decreased compared to the controls with the decrease of carbon fixation strength of alga. As for ON and OP groups, when they were in the range of experimental concentration, decreasing trends of inorganic carbon in sea water were always found. The concentration changes of DIC (DeltaDIC) under the press of the four typical organic pollutants were significantly negatively correlated with the changes of dry weight of alga (p < 0.01 or p < 0.05) and the coefficient parameters were -0.902, -0.945, -0.898 and -0.918, respectively (t = 7 d). The main controlling factor to the variation of carbon dioxided system was the adaption of biological carbon fixed system to different concentration and kind of organic pollutants.