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Sheng Wu Gong Cheng Xue Bao ; 40(4): 1076-1088, 2024 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-38658150

RESUMO

Flavin-containing monooxygenase (FMO) is the key enzyme in the biosynthesis pathway of CSOs with sulfur oxidation. In order to explore the molecular regulatory mechanism of FMO in the synthesis of onion CSOs, based on transcriptome database and phylogenetic analysis, one AcFMO gene that may be involved in alliin synthesis was obtained, the AcFMO had a cDNA of 1 374 bp and encoded 457 amino acids, which was evolutionarily closest to the AsFMO of garlic. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) indicated that AcFMO was the highest in the flowers and the lowest in the leaf sheaths. The results of subcellular localization showed that the AcFMO gene product was widely distributed throughout the cell A yeast expression vector was constructed, and the AcFMO gene was ecotopically overexpressed in yeast to further study the enzyme function in vitro and could catalyze the synthesis of alliin by S-allyl-l-cysteine. In summary, the cloning and functional identification of AcFMO have important reference value for understanding the biosynthesis of CSOs in onions.


Assuntos
Clonagem Molecular , Cisteína/análogos & derivados , Cebolas , Cebolas/genética , Cebolas/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Cisteína/biossíntese , Cisteína/metabolismo , Oxigenases/genética , Oxigenases/metabolismo , Sequência de Aminoácidos , Filogenia , Dissulfetos/metabolismo , Dados de Sequência Molecular , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo
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