RESUMO
Ecdysone-induced protein 93 (E93), known as the 'adult-specifier' transcription factor in insects, triggers metamorphosis in both hemimetabolous and holometabolous insects. Although E93 is conserved in ametabolous insects, its spatiotemporal expression and physiological function remain poorly understood. In this study, we first discover that, in the ametabolous firebrat Thermobia domestica, the previtellogenic ovary exhibits cyclically high E93 expression, and E93 mRNA is broadly distributed in previtellogenic ovarioles. E93 homozygous mutant females of T. domestica exhibit severe fecundity deficiency due to impaired previtellogenic development of the ovarian follicles, likely because E93 induces the expression of genes involved in ECM (extracellular matrix)-receptor interactions during previtellogenesis. Moreover, we reveal that in the hemimetabolous cockroach Blattella germanica, E93 similarly promotes previtellogenic ovarian development. In addition, E93 is also essential for vitellogenesis that is necessary to guarantee ovarian maturation and promotes the vitellogenesis-previtellogenesis switch in the fat body of adult female cockroaches. Our findings deepen the understanding of the roles of E93 in controlling reproduction in insects, and of E93 expression and functional evolution, which are proposed to have made crucial contributions to the origin of insect metamorphosis.
Assuntos
Metamorfose Biológica , Ovário , Reprodução , Animais , Feminino , Reprodução/genética , Metamorfose Biológica/genética , Ovário/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Vitelogênese/genética , Proteínas de Insetos/metabolismo , Proteínas de Insetos/genética , Fatores de Transcrição/metabolismo , Fatores de Transcrição/genética , Proteínas de Drosophila/metabolismo , Proteínas de Drosophila/genéticaRESUMO
BACKGROUND: Cockroaches are widely acknowledged as significant vectors of pathogenic microorganisms. The Periplaneta fuliginosa densovirus (PfDNV) infects the smoky-brown cockroach P. fuliginosa and causes host mortality, which identifies the PfDNV as a species-specific and environmentally friendly biopesticide. However, although the biochemical characterization of PfDNV has been extensively studied, the immune response against PfDNV remains largely unclear. RESULTS: Here, we investigated the replication of PfDNV and its associated pathological phenotype in the foregut and hindgut. Consequently, we dissected and performed transcriptome sequencing on the foregut, midgut, and hindgut separately. We revealed the up-regulation of immune response signaling pathway c-Jun N-terminal kinase (JNK) and apoptosis in response to viral infection. Furthermore, knockdown of the JNK upstream gene Ben resulted in a decrease in virus titer and delayed host mortality. CONCLUSION: Taken together, our findings provide evidence that the Ben-JNK signaling plays a crucial role in PfDNV infection, leading to excessive apoptosis in intestinal tissues and ultimately resulting in the death of the host. Our results indicated that the host response to PfDNV fosters viral infection, thereby increasing host lethality. This underscores the potential of PfDNV as a viable, environmentally friendly biopesticide. © 2024 Society of Chemical Industry.
Assuntos
Densovirus , Periplaneta , Animais , Periplaneta/virologia , Periplaneta/fisiologia , Periplaneta/genética , Densovirus/fisiologia , Densovirus/genética , Proteínas de Insetos/metabolismo , Proteínas de Insetos/genética , Sistema de Sinalização das MAP Quinases , Apoptose , Transdução de SinaisRESUMO
Volatile sex pheromones are vital for sexual communication between males and females. Females of the American cockroach, Periplaneta americana, produce and emit two sex pheromone components, periplanone-A (PA) and periplanone-B (PB). Although PB is the major sex attractant and can attract males, how it interacts with PA in regulating sexual behaviors is still unknown. In this study, we found that in male cockroaches, PA counteracted PB attraction. We identified two odorant receptors (ORs), OR53 and OR100, as PB/PA and PA receptors, respectively. OR53 and OR100 were predominantly expressed in the antennae of sexually mature males, and their expression levels were regulated by the sex differentiation pathway and nutrition-responsive signals. Cellular localization of OR53 and OR100 in male antennae further revealed that two types of sensilla coordinate a complex two-pheromone-two-receptor pathway in regulating cockroach sexual behaviors. These findings indicate distinct functions of the two sex pheromone components, identify their receptors and possible regulatory mechanisms underlying the male-specific and age-dependent sexual behaviors, and can guide novel strategies for pest management.
Assuntos
Periplaneta , Receptores Odorantes , Atrativos Sexuais , Comportamento Sexual Animal , Animais , Masculino , Atrativos Sexuais/metabolismo , Feminino , Receptores Odorantes/metabolismo , Receptores Odorantes/genética , Periplaneta/metabolismo , Periplaneta/fisiologia , Periplaneta/genética , Comportamento Sexual Animal/fisiologia , Antenas de Artrópodes/metabolismo , Antenas de Artrópodes/fisiologia , Comunicação Animal , Proteínas de Insetos/metabolismo , Proteínas de Insetos/genética , Receptores de Feromônios/metabolismo , Receptores de Feromônios/genéticaRESUMO
Appendage regeneration relies on the formation of blastema, a heterogeneous cellular structure formed at the injury site. However, little is known about the early injury-activated signaling pathways that trigger blastema formation during appendage regeneration. Here, we provide compelling evidence that the extracellular signal-regulated kinase (ERK)-activated casein kinase 2 (CK-2), which has not been previously implicated in appendage regeneration, triggers blastema formation during leg regeneration in the American cockroach, Periplaneta americana. After amputation, CK-2 undergoes rapid activation through ERK-induced phosphorylation within blastema cells. RNAi knockdown of CK-2 severely impairs blastema formation by repressing cell proliferation through down-regulating mitosis-related genes. Evolutionarily, the regenerative role of CK-2 is conserved in zebrafish caudal fin regeneration via promoting blastema cell proliferation. Together, we find and demonstrate that the ERK-activated CK-2 triggers blastema formation in both cockroach and zebrafish, helping explore initiation factors during appendage regeneration.
Assuntos
Regeneração , Peixe-Zebra , Animais , Peixe-Zebra/metabolismo , Regeneração/genética , Cicatrização , Transdução de Sinais/genética , Proteínas de Peixe-Zebra/genética , Proteínas de Peixe-Zebra/metabolismoRESUMO
The mystery of appendage regeneration has fascinated humans for centuries, while the underlying regulatory mechanisms remain unclear. In this study, we establish a transcriptional landscape of regenerating leg in the American cockroach, Periplaneta americana, an ideal model in appendage regeneration studies showing remarkable regeneration capacity. Through a large-scale in vivo screening, we identify multiple signaling pathways and transcription factors controlling leg regeneration. Specifically, zfh-2 and bowl contribute to blastema cell proliferation and morphogenesis in two transcriptional cascades: bone morphogenetic protein (BMP)/JAK-STAT-zfh-2-B-H2 and Notch-drm/bowl-bab1. Notably, we find zfh-2 is working as a direct target of BMP signaling to promote cell proliferation in the blastema. These mechanisms might be conserved in the appendage regeneration of vertebrates from an evolutionary perspective. Overall, our findings reveal that two crucial transcriptional cascades orchestrate distinct cockroach leg regeneration processes, significantly advancing the comprehension of molecular mechanism in appendage regeneration.
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Baratas , Animais , Humanos , Fatores de Transcrição , MorfogêneseRESUMO
DNA methylation at the fifth position of cytosine (5-methylcytosine, 5mC) is a crucial epigenetic modification for regulating gene expression, but little is known about how it regulates gene expression in insects. Here, we pursue the detailed molecular mechanism by which DNMT1-mediated 5mC maintenance regulates female reproduction in the German cockroach, Blattella germanica. Our results show that Dnmt1 knockdown decreases the level of 5mC in the ovary, upregulating numerous genes during choriogenesis, especially the transcription factor ftz-f1. The hypomethylation at the ftz-f1 promoter region increases and prolongs ftz-f1 expression in ovarian follicle cells during choriogenesis, which consequently causes aberrantly high levels of 20-hydroxyecdysone and excessively upregulates the extracellular matrix remodeling gene Mmp1. These changes further impair choriogenesis and disrupt fertilization by causing anoikis of the follicle cells, a shortage of chorion proteins, and malformation of the sponge-like bodies. This study significantly advances our understanding of how DNA 5mC modification regulates female reproduction in insects.
Assuntos
Proteínas de Ligação a DNA , Fatores de Transcrição , Animais , Feminino , Proteínas de Ligação a DNA/metabolismo , Fatores de Transcrição/metabolismo , Regulação da Expressão Gênica , Insetos/metabolismo , Fertilização/genéticaRESUMO
Phosphorylation is a key post-translational modification in regulating autophagy in yeast and mammalians, yet it is not fully illustrated in invertebrates such as insects. ULK1/Atg1 is a functionally conserved serine/threonine protein kinase involved in autophagosome initiation. As a result of alternative splicing, Atg1 in the silkworm, Bombyx mori, is present as three mRNA isoforms, with BmAtg1c showing the highest expression levels. Here, we found that BmAtg1c mRNA expression, BmAtg1c protein expression and phosphorylation, and autophagy simultaneously peaked in the fat body during larval-pupal metamorphosis. Importantly, two BmAtg1c phosphorylation sites were identified at Ser269 and Ser270, which were activated by BmAMPK, the major energy-sensing kinase, upon stimulation with 20-hydroxyecdysone and starvation; additionally, these Atg1 phosphorylation sites are evolutionarily conserved in insects. The two BmAMPK-activated phosphorylation sites in BmAtg1c were found to be required for BmAMPK-induced autophagy. Moreover, the two corresponding DmAtg1 phosphorylation sites in the fruit fly, Drosophila melanogaster, are functionally conserved for autophagy induction. In conclusion, AMPK-activated Atg1 phosphorylation is indispensable for autophagy induction and evolutionarily conserved in insects, shedding light on how various groups of organisms differentially regulate ULK1/Atg1 phosphorylation for autophagy induction.
Assuntos
Proteínas Quinases Ativadas por AMP , Proteínas de Drosophila , Animais , Fosforilação , Proteínas Quinases Ativadas por AMP/genética , Proteínas Quinases Ativadas por AMP/metabolismo , Drosophila melanogaster/metabolismo , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismo , Drosophila/metabolismo , Autofagia/genética , Mamíferos/metabolismo , Proteína Homóloga à Proteína-1 Relacionada à Autofagia/genética , Proteína Homóloga à Proteína-1 Relacionada à Autofagia/metabolismo , Proteínas de Drosophila/metabolismoRESUMO
Juvenile hormone (JH) and 20-hydroxyecdysone (20E) coordinately regulate development and metamorphosis in insects. Two JH intracellular receptors, methoprene-tolerant (Met) and germ-cell expressed (Gce), have been identified in the fruit fly Drosophila melanogaster. To investigate JH membrane signaling pathway without the interference from JH intracellular signaling, we characterized phosphoproteome profiles of the Met gce double mutant in the absence or presence of JH in both chronic and acute phases. Functioning through a potential receptor tyrosine kinase and phospholipase C pathway, JH membrane signaling activated protein kinase C (PKC) which phosphorylated ultraspiracle (USP) at Ser35, the PKC phosphorylation site required for the maximal action of 20E through its nuclear receptor complex EcR-USP. The uspS35A mutant, in which Ser was replaced with Ala at position 35 by genome editing, showed decreased expression of Halloween genes that are responsible for ecdysone biosynthesis and thus attenuated 20E signaling that delayed developmental timing. The uspS35A mutant also showed lower Yorkie activity that reduced body size. Altogether, JH membrane signaling phosphorylates USP at Ser35 and thus potentiates 20E action that regulates the normal fly development. This study helps better understand the complex JH signaling network.
Assuntos
Drosophila , Hormônios Juvenis , Animais , Hormônios Juvenis/genética , Drosophila/metabolismo , Ecdisterona/farmacologia , Drosophila melanogaster/metabolismo , Transdução de Sinais , Metoprene/farmacologia , Proteína Quinase C/genéticaRESUMO
Insects have evolved numerous adaptations and colonized diverse terrestrial environments. Several polyneopterans, including dictyopterans (cockroaches and mantids) and locusts, have developed oothecae, but little is known about the molecular mechanism, physiological function, and evolutionary significance of ootheca formation. Here, we demonstrate that the cockroach asymmetric colleterial glands produce vitellogenins, proline-rich protein, and glycine-rich protein as major ootheca structural proteins (OSPs) that undergo sclerotization and melanization for ootheca formation through the cooperative protocatechuic acid pathway and dopachrome and dopaminechrome subpathway. Functionally, OSP sclerotization and melanization prevent eggs from losing water at warm and dry conditions, and thus effectively maintain embryo viability. Dictyopterans and locusts convergently evolved vitellogenins, apolipoprotein D, and laminins as OSPs, whereas within Dictyoptera, cockroaches and mantids independently developed glycine-rich protein and fibroins as OSPs. Highlighting the ecological-evolutionary importance, convergent ootheca formation represents a successful reproductive strategy in Polyneoptera that promoted the radiation and establishment of cockroaches, mantids, and locusts.
Assuntos
Baratas , Besouros , Aclimatação , Animais , Insetos , ReproduçãoRESUMO
Transmissible gastroenteritis virus (TGEV) is a coronavirus (CoV) that is a major pathogenity of viral enteritis and diarrhea in suckling piglets, causing high morbidity and mortality. In this study, a TGEV strain HQ2016 was isolated from northeast China and characterized its genome sequence and pathogenicity. The phylogenetic analysis indicated that the TGEV HQ2016 strain was more similar to the TGEV Purdue cluster than to the Miller cluster. Both recombination and phylogenetic analysis based on each structural and non-structural gene revealed no recombination event in the HQ2016 strain. Experimental infection study using colostrum-deprived newborn piglets successfully showed that the HQ2016 can cause clinical symptoms including anorexia and yellow-to-whitish watery diarrhea, which are characteristics of TGE, in the inoculated piglets 48 h post-inoculation. These results provide valuable information about the evolution of the porcine CoVs.
RESUMO
In insects, 20-hydroxyecdysone (20E) limits systemic growth by triggering developmental transitions. Previous studies have shown that 20E-induced let-7 exhibits crosstalk with the cell cycle. Here, we examined the underlying molecular mechanisms and physiological functions of 20E-induced let-7 in the fat body, an organ for energy storage and nutrient mobilization which plays a critical role in the larval growth. First, the overexpression of let-7 decreased the body size and led to the reduction of both nucleolus and cell sizes in the larval fat body. In contrast, the overexpression of let-7-Sponge increased the nucleolus and cell sizes. Moreover, we found that cdc7, encoding a conserved protein kinase that controls the endocycle, is a target of let-7. Notably, the mutation of cdc7 in the fat body resulted in growth defects. Overall, our findings revealed a novel role of let-7 in the control of endoreduplication-related growth during larval-prepupal transition in Drosophila.
Assuntos
Proteínas de Drosophila , Drosophila/crescimento & desenvolvimento , Corpo Adiposo , MicroRNAs , Proteínas Serina-Treonina Quinases , Animais , Drosophila/genética , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Ecdisterona , Corpo Adiposo/metabolismo , Larva , MicroRNAs/genética , Proteínas Serina-Treonina Quinases/genética , Proteínas Serina-Treonina Quinases/metabolismoRESUMO
In insects, 20-hydroxyecdysone (20E) limits the growth period by triggering developmental transitions; 20E also modulates the growth rate by antagonizing insulin/insulin-like growth factor signaling (IIS). Previous work has shown that 20E cross-talks with IIS, but the underlying molecular mechanisms are not fully understood. Here we found that, in both the silkworm Bombyx mori and the fruit fly Drosophila melanogaster, 20E antagonized IIS through the AMP-activated protein kinase (AMPK)-protein phosphatase 2A (PP2A) axis in the fat body and suppressed the growth rate. During Bombyx larval molt or Drosophila pupariation, high levels of 20E activate AMPK, a molecular sensor that maintains energy homeostasis in the insect fat body. In turn, AMPK activates PP2A, which further dephosphorylates insulin receptor and protein kinase B (AKT), thus inhibiting IIS. Activation of the AMPK-PP2A axis and inhibition of IIS in the Drosophila fat body reduced food consumption, resulting in the restriction of growth rate and body weight. Overall, our study revealed an important mechanism by which 20E antagonizes IIS in the insect fat body to restrict the larval growth rate, thereby expanding our understanding of the comprehensive regulatory mechanisms of final body size in animals.
Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Tamanho Corporal/fisiologia , Proteína Fosfatase 2/metabolismo , Animais , Bombyx/crescimento & desenvolvimento , Bombyx/metabolismo , Drosophila/crescimento & desenvolvimento , Drosophila/metabolismo , Ecdisterona/metabolismo , Corpo Adiposo/metabolismo , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Proteínas de Insetos/genética , Insetos/crescimento & desenvolvimento , Insetos/metabolismo , Insulina/metabolismo , Larva/crescimento & desenvolvimento , Receptor de Insulina/metabolismo , Transdução de Sinais/efeitos dos fármacos , Somatomedinas/metabolismoRESUMO
Porcine epidemic diarrhoea virus (PEDV) is an emerging and re-emerging epizootic virus of swine that causes substantial economic losses to the pig industry in China and other countries. The variations in the virus, and its co-infections with other enteric viruses, have contributed to the poor control of PEDV infection. In the current study, a broad epidemiological investigation of PEDV was carried out in 22 provinces or municipalities of China during 2015-2018. The enteric viruses causing co-infection with PEDV and the genetic diversity of the PEDV S1 gene were also analysed. The results indicated that, of the 543 diarrhoea samples, 66.85% (363/543) were positive for PEDV, and co-infection rates of PEDV with 13 enteric viruses ranged from 3.58% (13/363) to 81.55% (296/363). Among these enteric viruses, the signs of diarrhoea induced by PEDV were potentially associated with co-infections with porcine enterovirus 9/10 (PEV) and torque teno sus virus 2 (TTSuV-2) (p < .05). The 147 PEDV strains identified in our study belong to Chinese pandemic strains and exhibited genetic diversity. The virulence-determining S1 proteins of PEDV pandemic strains were undergoing amino acid mutations, in which S58_S58insQGVN-N135dup-D158_I159del-like mutations were common patterns (97.28%, 143/147). When compared with 2011-2014 PEDV strains, the amino acid mutations of PEDV pandemic strains were mainly located in the N-terminal domain of S1 (S1-NTD), and 21 novel mutations occurred in 2017 and 2018. Furthermore, protein homology modelling showed that the mutations in pattern of insertion and deletion mutations of the S1 protein of PEDV pandemic strains may have caused structural changes on the surface of the S1 protein. These data provide a better understanding of the co-infection and genetic evolution of PEDV in China.
Assuntos
Coinfecção/veterinária , Infecções por Coronavirus/veterinária , Variação Genética , Vírus da Diarreia Epidêmica Suína/isolamento & purificação , Doenças dos Suínos/epidemiologia , Proteínas Virais/genética , Sequência de Aminoácidos , Animais , China/epidemiologia , Coinfecção/epidemiologia , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Filogenia , Vírus da Diarreia Epidêmica Suína/genética , Alinhamento de Sequência , Suínos , Doenças dos Suínos/virologia , Proteínas Virais/química , Proteínas Virais/metabolismo , Viroses/epidemiologia , Viroses/veterinária , Viroses/virologiaRESUMO
Cholesterol plays essential roles in animal development and disease progression. Here, we characterize the evolutionary pattern of the canonical cholesterol biosynthesis pathway (CBP) in the animal kingdom using both genome-wide analyses and functional experiments. CBP genes in the basal metazoans were inherited from their last common eukaryotic ancestor and evolutionarily conserved for cholesterol biosynthesis. The genomes of both the basal metazoans and deuterostomes retain almost the full set of CBP genes, while Cnidaria and many protostomes have independently experienced multiple massive losses of CBP genes that might be due to the geologic events during the Ediacaran period, such as the appearance of an exogenous sterol supply and the frequent perturbation of ocean oxygenation. Meanwhile, the indispensable utilization processes of cholesterol potentially strengthened the maintenance of the complete set of CBP genes in vertebrates. These results strengthen both biotic and abiotic roles in the macroevolution of a biosynthesis pathway in animals.
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Thitarodes (Lepidoptera, Hepialidae) is the only genus that hosts to the Ophiocordyceps sinensis, a traditional Chinese medicine considered as a powerful medicinal supplement. In this study, the complete mitochondrial genomes (mitogenomes) of two species, T. damxungensis and T. pui, have been sequenced, which are 15,928â¯bp and 15,362â¯bp in size respectively, and both contain 13 protein-coding genes (PCGs), 2 rRNAs, 22 tRNAs and an AT-rich region. Like other hepialoids, the gene arrangement of the mitogenomes of T. damxungensis and T. pui is identical to the ancestral arrangement but differs from those of other lepidopteran species on account of the different arrangements of trnM, trnI, and trnQ. The size of AT-rich region is 545â¯bp in T. damxungensis and 1030â¯bp in T. pui. Tandem repetition in the AT-rich region is responsible for the length difference of the Aâ¯+â¯T-rich region in both species. In Hepialidae, the phylogenetic study based on the dataset of the sequences that combined the protein-coding genes and RNA genes suggested that the species T. yunnanensis should still belong to the genus Thitarodes rather than Ahamns, which is different from the results based on the traditional phylogeny.
Assuntos
Ascomicetos , Genoma Mitocondrial , Genômica , Lepidópteros/genética , Lepidópteros/microbiologia , Animais , Composição de Bases , Códon , Biologia Computacional/métodos , Genes de Insetos , Genômica/métodos , Lepidópteros/classificação , Anotação de Sequência Molecular , Fases de Leitura Aberta , FilogeniaRESUMO
As an intrinsic property, elasticity of soft material is affected significantly by the externally applied alternating magnetic field. Magnetostrictive properties of the grain-oriented (GO) silicon steel under DC-biased and multisinusoidal magnetizations are measured by using a laser-based measuring system. Magnetostriction curves of the GO silicon steel sheet under different magnetizations are obtained and the influence of frequency and DC bias on the magnetostrictive property is observed and analyzed based on the measured data. In addition, the spectrum of magnetostriction under harmonic magnetization is obtained, and the acoustic noise level of the GO silicon steel sheet represented by the A-weighted decibel value caused by magnetostriction is measured under DC-biased and multisinusoidal magnetizations. The measurement results are applied to the simulation of the three-limb laminated core model, and the effects of DC bias and harmonics on magnetic flux density and displacement are analyzed.
RESUMO
To trace the prevalence of canine distemper virus (CDV) in diarrhoetic dogs, a total of 201 stool samples were collected in the Heilongjiang province of northeastern China from May 2014 to April 2015. The 201 fecal samples were subjected to the detection of CDV by using RT-PCR targeting the partial N gene, phylogenetic analysis based on the complete H gene, and co-infection analysis. Results indicated that 24.88% (50/201) of the samples were positive for CDV. The fifty CDV samples exhibited an overall co-infection rate of 94% (47/50) with four enteric viruses (82%, 41/50) and five bacteria (72%, 36/50). The positivity rate of CDV exhibited differences among regions, seasons, ages and immunization status. Phylogenetic analysis of the complete H genes (n=6) revealed that the CDV strains identified in our study belonged to the Asia-1 group, and showed genetic diversities. These data provide evidence that there are a number of genetically diverse CDV Asia-1 strains circulating in diarrhoetic dogs in northeastern China; the CDV-affected animals exhibit the high co-infection with other enteric viruses and bacteria.
Assuntos
Vírus da Cinomose Canina/genética , Cinomose/virologia , Doenças do Cão/virologia , Animais , China , Cães , Escherichia coli , Feminino , FilogeniaRESUMO
Many cockroach species have adapted to urban environments, and some have been serious pests of public health in the tropics and subtropics. Here, we present the 3.38-Gb genome and a consensus gene set of the American cockroach, Periplaneta americana. We report insights from both genomic and functional investigations into the underlying basis of its adaptation to urban environments and developmental plasticity. In comparison with other insects, expansions of gene families in P. americana exist for most core gene families likely associated with environmental adaptation, such as chemoreception and detoxification. Multiple pathways regulating metamorphic development are well conserved, and RNAi experiments inform on key roles of 20-hydroxyecdysone, juvenile hormone, insulin, and decapentaplegic signals in regulating plasticity. Our analyses reveal a high level of sequence identity in genes between the American cockroach and two termite species, advancing it as a valuable model to study the evolutionary relationships between cockroaches and termites.
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Adaptação Biológica/fisiologia , Genoma , Genômica , Metamorfose Biológica/fisiologia , Periplaneta/fisiologia , Animais , Ecdisterona/fisiologia , Meio Ambiente , Feminino , Proteínas de Insetos/fisiologia , Insulina/fisiologia , Isópteros/genética , Hormônios Juvenis/fisiologia , Masculino , Filogenia , Interferência de RNA , Transdução de Sinais/fisiologia , Transcriptoma , Sequenciamento Completo do GenomaRESUMO
Recently, parainfluenza virus 5 (PIV5) infection has been increasingly reported in mammals. In this study, five PIV5 strains were isolated from diarrhea-affected piglets from four provinces or municipalities in China. An F-gene-based phylogenetic tree indicated that the five isolated strains were closely related to the PIV5 strain ZJQ-221 from a lesser panda in China, and the PIV5 strain 1168-1 from a dog in South Korea. The new isolates differed genetically from other pig, calf, rhesus macaque kidney cells, human, and dog PIV5 reference strains. Our study reveals the presence of PIV5 in intestinal tissue samples collected from diarrhea-affected piglets, and provides novel information regarding the epidemiology and tissue tropism of PIV5.
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Diarreia/veterinária , Vírus da Parainfluenza 5/isolamento & purificação , Infecções por Rubulavirus/veterinária , Doenças dos Suínos/virologia , Animais , China/epidemiologia , Diarreia/epidemiologia , Diarreia/virologia , Vírus da Parainfluenza 5/genética , Infecções por Rubulavirus/epidemiologia , Infecções por Rubulavirus/virologia , Suínos , Doenças dos Suínos/epidemiologiaRESUMO
Rabbit hemorrhagic disease (RHD) is a contagious disease in adult rabbits, with high mortality, that occurs throughout the world. The VP60 protein has been implicated as main protein antigen in virus diagnosis and vaccine design. In this report, we describe the construction of a novel DNA vaccine (pcDNA-VP60) expressing the RHDV capsid protein (VP60), and the expression of the recombinant protein was identified through indirect immunofluorescence assay (IFA) and Western blot assay. VP60 protein self-assembled to form virus-like particles (VLPs) observed by electron microscopy were morphologically similar to native virions. For the evaluation of vaccine efficacy, rabbits were inoculated with PBS, pcDNA3.1((+)), pcDNA-VP60 or RHDV inactive vaccine. They were challenged with RHDV-TP isolate four weeks after last boost immunization. In all cases, the rabbits immunized with pcDNA-VP60 developed high level of RHDV-specific antibodies and cellular immune response. The rabbits injected with DNA vaccine were completely protected against RHDV challenge like commercial RHDV inactive vaccine, moreover, RHDV viral load was significantly reduced in the liver samples from immunized rabbits. The recombinant DNA vaccine may provide a novel strategy for the immunization of rabbits for the control of RHDV.