A novel mice model of metabolic syndrome: the high-fat-high-fructose diet-fed ICR mice.

Currently, the metabolic syndrome (MS) is occurring at growing rates worldwide, raising extensive concerns on the mechanisms and therapeutic interventions for this disorder. Herein, we described a novel method of establishing MS model in rodents. Male Institute of Cancer Research (ICR) mice were fed with high-fat-high-fructose (HFHF) diet or normal chow (NC) respectively for 12 weeks. Metabolic phenotypes were assessed by glucose tolerance test, insulin tolerance test and hyperinsulinemic-euglycemic clamp. Blood pressure was measured by a tail-cuff system. At the end of the experiment, mice were sacrificed, and blood and tissues were harvested for subsequent analysis. Serum insulin levels were measured by ELISA, and lipid profiles were determined biochemically. The HFHF diet-fed ICR mice exhibited obvious characteristics of the components of MS, including obvious obesity, severe insulin resistance, hyperinsulinemia, dislipidemia, significant hypertension and hyperuricemia. Our data suggest that HFHF diet-fed ICR mice may be a robust and efficient animal model that could well mimic the basic pathogenesis of human MS.

A novel method for wound healing-skin suspension accelerates donor site wound healing .

UNLABELLED:  The purpose of this study was to apply a skin suspension to accelerate wound healing at the skin donor site. METHODS: A small fragment of skin was collected after skin transplantation had been performed. The skin suspension was prepared by mixing the small fragments of skin tissue with the same volume of normal saline. The suspension was then applied to the donor site. Donor sites without skin suspension were employed as controls. RESULTS: Faster healing was found at the donor sites that had been covered with skin suspension and with less scar formation compared to controls. CONCLUSION: Skin suspension prepared from residual graft skin can ameliorate donor site wound healing.