Gold nanoparticles improve the embryonic developmental competency of artificially activated mouse oocytes.

Currently, artificial oocyte activation has attracted wide attention in assisted reproduction due to extensive range of applications, particularly in somatic cell nuclear transfer and deriving pluripotent stem cell lines and it is the unique model to determine the role of paternal genome. Numbers of artificial activating agents have been used extensively to induce the oocytes activation; however, embryos developmental competency of artificially activated oocytes is still very low. In the present study, we determined the functional impact of strontium chloride supplementation with gold nanoparticles (AuNPs) in artificial oocytes activation and subsequent embryonic development. Oocytes were activated artificially in the culture medium containing 250 nM AuNPs with constant concentration of strontium chloride 10.00 mM. We found that adding 250 nM AuNPs with constant concentration of strontium chloride (10.00 mM for 3 hr) in culture medium improves the proportion of embryos reaching to the morula and blastocyst stages from 61.00% and 42.00% (controls) to 75.00% and 58.00% (250 nM AuNPs), respectively. In addition, foster mothers receiving AuNPs-treated embryos showed more implantation percentage and pregnancy rate relative to females received control embryos. Finally, embryos treated with 250 nM AuNPs concentration showed no toxic effect in term of blastocyst development. Collectively, our findings suggest the potential role of AuNPs in early embryonic development for mouse oocytes activated artificially and provide new insights in the field of animal biotechnology and assisted reproduction in humans.

Biomass-derived carbon boosted catalytic properties of tungsten-based nanohybrids for accelerating the triiodide reduction in dye-sensitized solar cells.

Manganese tungstate (MnWO4), zinc tungstate (ZnWO4), and copper tungstate (CuWO4) embedded biomass-derived carbon (MWO-C, ZWO-C, CWO-C) was synthesized by hydrothermal treatment and investigated as counter electrode (CE) catalysts to test electrochemical activity. Biomass-derived carbon was used as the shape controlling agent, which changed the morphology of MWO from spherical to spindle-like. Owing to the synergistic effect between tungsten-based bimetal oxides and biomass-derived carbon, the MWO-C, ZWO-C, and CWO-C catalysts exhibited enhanced electrochemical performance in dye-sensitized solar cells (DSSCs) system. The MWO-C, ZWO-C and CWO-C catalysts in DSSCs showed outstanding power conversion efficiency (PCE) of 7.33%, 7.61%, and 6.52%, respectively, as compared with 7.04% for Pt based devices. Biomass-derived carbon improves the catalytic properties of tungsten-based nanohybrids. The results showed that biomass-derived carbon-enhanced inorganic compound as CE catalysts are promising alternatives to Pt-based CE catalysts for energy conversion devices.