RESUMO
Paracoccidioidomycosis (PCM) is a systemic mycosis prevalent in Brazil and other Latin American countries. The etiological agents of PCM are the thermo-dimorphic fungi Paracoccidioides brasiliensis and P. lutzii. Taking into account that the natural habitat of Paracoccidioides spp. is still undefined, domestic and wild animals could be useful as indicators of Paracoccidioides spp. presence in endemic areas. The objective of this study was to evaluate the infection of small wild mammals by P. brasiliensis in an endemic area for human PCM. Samples from 38 wild mammals from different species such as Akodon sp., Thaptomys nigrita, Euryoryzomys russatus, Oligoryzomys nigripes, Monodelphis sp., Sooretamys angouya, Abrawayaomys angouya, Abrawayaomys ruschii and Akodontinae sp. were evaluated by ELISA, immunodiffusion, histopathology, nested PCR and culture. The overall positivity to gp43 observed in the ELISA was 23.7%. Samples from heart and liver of one O. nigripes were PCR positive, and the animal was also seropositive to gp43 in ELISA. This study showed that wild animals living in endemic areas for PCM are infected with P. brasiliensis and can be valuable epidemiological markers of the fungus presence in the environment. This is the first evidence of PCM infection in Akodon sp., E. russatus, T. nigrita and O. nigripes.
Assuntos
Monodelphis , Paracoccidioides/isolamento & purificação , Paracoccidioidomicose/veterinária , Sigmodontinae , Animais , Anticorpos Antifúngicos/sangue , Brasil , DNA Fúngico/análise , DNA Fúngico/genética , Ensaio de Imunoadsorção Enzimática , Coração/microbiologia , Fígado/microbiologia , Paracoccidioidomicose/epidemiologia , Paracoccidioidomicose/microbiologia , Reação em Cadeia da Polimerase , PrevalênciaRESUMO
Here we present tabular keys for families and genera of ascidian species found in shallow waters in the Atlantic Ocean. Characters used in the keys are illustrated or shown in photos of dissected animals and we also provide generalized drawings of all genera. A list of species in each genus and their geographical distribution in the Atlantic is also included, totaling 461 species.
O trabalho apresenta chaves tabulares para famílias e gêneros de espécies de ascídias encontradas em águas rasas no Oceano Atlântico. Os caracteres usados nas chaves são ilustrados ou apresentados em fotos de animais dissecados e são apresentados também desenhos generalizados de todos os gêneros. Também foram incluídas listas de espécies em cada gênero e sua distribuição geográfica no Atlântico, totalizando 461 espécies.