Harmful planktonic Microcystis and benthic Oscillatoria-induced toxicological effects on the Asian clam (Corbicula fluminea): A survey on histopathology, behavior, oxidative stress, apoptosis and inflammation.

Cyanobacterial blooms are worldwide distributed and threaten aquatic ecosystems and public health. The current studies mainly focus on the adverse impacts of planktonic cyanobacteria or pure cyanotoxins, while the benthic cyanobacteria-induced ecotoxic effects are relatively lacking. The cyanobacterial cell-induced toxic effects on aquatic organisms might be more serious and complex than the pure cyanotoxins and crude extracts of cyanobacteria. This study explored the chronic effects of toxin-producing planktonic Microcystis aeruginosa (producing microcystin) and benthic Oscillatoria sp. (producing cylindrospermopsin) on the behaviors, tissue structures, oxidative stress, apoptosis, and inflammation of the Asian clams (Corbicula fluminea) under 28-d exposure. The data showed that both M. aeruginosa and Oscillatoria sp. can decrease the behaviors associated with the feeding activity and induce tissue damage (i.e. gill and digestive gland) in clams. Furthermore, two kinds of cyanobacteria can alter the antioxidant enzyme activities and increase antioxidant, lipid oxidation product, and neurotransmitter degrading enzyme levels in clams. Moreover, two kinds of cyanobacteria can activate apoptosis-related enzyme activities and enhance the proinflammatory cytokine levels of clams. In addition, two kinds of cyanobacteria can disturb the transcript levels of genes linked with oxidative stress, apoptosis, and inflammation. These results suggested harmful cyanobacteria can threaten the survival and health of clams, while the benthic cyanobacteria-induced adverse effects deserve more attention. Our finding also indicated that it is necessary to focus on the entire algal cell-induced ecotoxicity when concerning the ecological impacts of cyanobacterial blooms.

Molecular, behavioral, and growth responses of juvenile yellow catfish (Tachysurus fulvidraco) exposed to carbamazepine.

Carbamazepine (CBZ) is an anticonvulsant medication used to treat epilepsy and bipolar disorder. Due to its persistence and low removal rate in wastewater treatment plants, it is frequently detected in the environment, raising concerns regarding its potential adverse effects on aquatic organisms and ecosystems. In this study, we aimed to assess the impact of CBZ on the behavior and growth of juvenile yellow catfish Tachysurus fulvidraco, a native and economically important species in China. Fish were exposed to CBZ at three concentrations of 1, 10, or 100 µg/L for 14 days. The fish exposed to 10 and 100 µg/L of CBZ exhibited decreased feeding, and a significant increase in cannibalistic tendencies was observed in fish exposed to 100 µg/L CBZ. Acetylcholinesterase activity was increased in the brain of fish exposed to 100 µg/L CBZ. CBZ also inhibited the growth of yellow catfish. To better elucidate mechanisms of toxicity, transcriptomics was conducted in both the brain and liver. In the brain, gene networks associated with neurotransmitter dysfunction were altered by CBZ, as well as networks associated with mitochondrial dysfunction and metabolism. In the liver, gene networks associated with the immune system were altered by CBZ. The current study improves comprehension of the sub-lethal effects of CBZ and reveals novel insight into molecular and biochemical pathways disrupted by CBZ, identifying putative key events associated with reduced growth and altered behavior. This study emphasizes the necessity for improved comprehension of the effects of pharmaceutical contaminants on fish at environmentally relevant levels.

Biotic and Abiotic Regulations of Carbon Fixation into Lacustrine Sediments with Different Nutrient Levels.

Lake sediments play a critical role in organic carbon (OC) conservation. However, the biogeochemical processes of the C cycle in lake ecosystems remain limitedly understood. In this study, Fe fractions and OC fractions, including total OC (TOC) and OC associated with iron oxides (TOCFeO), were measured for sediments from a eutrophic lake in China. The abundance and composition of bacterial communities encoding genes cbbL and cbbM were obtained by using high-throughput sequencing. We found that autochthonous algae with a low C/N ratio together with δ13C values predominantly contributed to the OC burial in sediments rather than terrigenous input. TOCFeO served as an important C sink deposited in the sediments. A significantly positive correlation (r = 0.92, p < 0.001) suggested the remarkable regulation of complexed FeO (Fep) on fixed TOC fractions, and the Fe redox shift triggered the loss of deposited OC. It should be noted that a significant correlation was not found between the absolute abundance of C-associating genera and TOC, as well as TOCFeO, and overlying water. Some rare genera, including Acidovora and Thiobacillus, served as keystone species and had a higher connected degree than the genera with high absolute abundance. These investigations synthetically concluded that the absolute abundance of functional genes did not dominate CO2 fixation into the sediments via photosynthesis catalyzed by the C-associating RuBisCO enzyme. That is, rare genera, together with high-abundance genera, control the C association and fixation in the sediments.

The toxicological effects of life-cycle exposure to harmful benthic cyanobacteria Oscillatoria on zebrafish growth and reproduction: A comparative study with planktonic Microcystis.

The risks of planktonic cyanobacteria blooms have been the focus of much scientific research, but studies on the ecotoxicological effects of benthic cyanobacteria are lagging. The impacts of cyanobacteria cells on fish populations might be more complex in contrast to purified cyanotoxins or cyanobacteria extracts. This study systematically compared the chronic effects of benthic Oscillatoria sp. (producing cylindrospermopsins) and planktonic Microcystis aeruginosa (producing microcystins) on the growth and reproduction of zebrafish through life-cycle exposure (5- 90 days post fertilization). The results showed that both Oscillatoria sp. and M. aeruginosa exposure caused growth inhibition and fecundity reduction in F0 generation by disrupting sex hormone levels, delayed ovarian and sperm development, and induced pathological lesions in zebrafish gonads. Furthermore, exposure to Oscillatoria sp. or M. aeruginosa in adult zebrafish increased mortality and teratogenicity in F1 embryos (without exposure), indicating a parental transmission effect of developmental toxicity. The difference was that M. aeruginosa exposure led to significant alterations in pathways, such as tissue development, redox processes, and steroid hormone synthesis. In contrast, Oscillatoria sp. exposure primarily disrupted the PPAR signaling pathway, cell adhesion molecules, and lipid transport pathways. Interestingly, the differentially expressed genes revealed that male fish were more sensitive to harmful cyanobacteria than females, whether exposed to Oscillatoria sp. or M. aeruginosa. These findings contribute to a better mechanistic understanding of the chronic toxic effects of distinct types of harmful cyanobacteria, suggesting that the ecological risk of benthic cyanobacteria requires further attention.

Omics techniques reveal the toxicity mechanisms of three antiepileptic drugs to juvenile zebrafish (Danio rerio) brain and liver.

Epilepsy, a neurological disorder, is characterized by seizures that are an appearance of excessive brain activity and is symptomatically treated with antiepileptic drugs (AEDs). Oxcarbazepine (OCBZ), lamotrigine (LTG), and carbamazepine (CBZ) are widely used AEDs in clinics and are very often detected in aquatic environments. However, neither the sub-lethal effects nor the specific mechanisms of these AEDs' action on the fish are well understood. In this study, juvenile zebrafish were exposed to a sub-lethal concentration (100 µg/L) of OCBZ, LTG, and CBZ for 28 d, after which indicators of oxidative stress (i.e. superoxide dismutase (SOD) activity, catalase (CAT) activity, and malondialdehyde (MDA) level) and neurotoxicity (i.e. acetylcholinesterase (AChE) activity, γ-aminobutyric acid (GABA) level, and glutamic acid (Glu) level) were measured. Brain SOD activity was significantly increased by three AEDs, while brain CAT activity was significantly inhibited by LTG and CBZ. Liver SOD activity was significantly enhanced by CBZ, and liver CAT activity was significantly induced by OCBZ and LTG. Liver MDA level was significantly increased by three AEDs. Brain AChE activity was significantly increased by LTG and CBZ, and brain GABA level was significantly enhanced by three AEDs. However, there were no significant alterations in the levels of MDA and Glu in zebrafish brain. To ascertain mechanisms of AEDs-induced toxicity, brain transcriptomics and liver metabolomics were conducted in zebrafish. The brain transcriptomics results showed that lots of differentially expressed genes (DEGs) were enriched in the sensory system, the immune system, the digestive system, the metabolic processes, and others in three AEDs treated groups. The metabolomics data indicated dysregulation of glycerophospholipid signaling and lipid homeostasis in zebrafish liver after three AEDs exposure. The overall results of this study improve understanding of the sub-lethal effects and potential molecular mechanisms of action of AEDs in fish.

A molting chemical cue (N-acetylglucosamine-6-phosphate) contributes to cannibalism of Chinese mitten crab Eriocheir sinensis.

Under high-density culture, cannibalism occurs frequently during the molting of the Chinese mitten crabs Eriocheir sinensis, resulting in a large reduction in production. We found that the leakage of molting fluid from sexually immature crabs informs conspecifics that they are in a molting process. This hypothesis was verified through metabolomics analyses combined with behavioral experiments. The GlcNAc-6-P was identified as a molting biomarker from the differential metabolites by non-targeted metabolomics. In addition, we found that the concentration of GlcNAc-6-P in the molting fluid was significantly higher than other molting metabolites at different molting stages, reaching 5.84 µmol L-1, indicating that the molting fluid was the source of GlcNAc-6-P. Moreover, the behavioral experiments showed that crabs were actively approached to high concentrations of GlcNAc-6-P (1 µmol L-1), but had no obvious choice tendency at different concentrations of UTP, 20-HE and low concentrations of GlcNAc-6-P (0.1 µmol L-1, 0.01 µmol L-1) compared with the control groups. In conclusion, that E. sinensis by sensing the concentration change of GlcNAc-6-P can locate the source of GlcNAc-6-P release and actively approach the high concentration GlcNAc-6-P area and attack the molting crab, causing cannibalism. Blocking the reception pathway of molting chemical cues in E. sinensis, thereby preventing the perception of signals originating from conspecifics' molting in the vicinity, could lead to a reduction in cannibalistic behavior and an increase in overall production. Additionally, this method presents a prospective solution for addressing cannibalism in other crustacean species where such behavior is prevalent.

Development and testing of a planktonic index of biotic integrity (P-IBI) for Lake Fuxian, China.

Lake Fuxian has the largest reserves of high-quality water resources in China, and understanding its ecological health status is the basis of its environmental protection. Based on a seasonal field investigation of the plankton community, we established a planktonic index of biotic integrity (P-IBI) evaluation system to assess the lake's ecosystem health. The biological integrity of Lake Fuxian was relatively good during winter and spring, but gradually deteriorated from summer to autumn. Areas with poor biological integrity were mainly distributed near tourist attractions along the lake's west coast. Redundancy analysis (RDA) was performed to explore the relationships between the P-IBI, its selected indicators, and the environmental variables. Water temperature (WT), pH, ammonia nitrogen (NH3-N), and dissolved oxygen (DO) significantly influenced the P-IBI and its selected indicators. NH3-N and DO were significantly positively correlated with the P-IBI, indicating that it could be used as a water quality indicator to indirectly reflect lake biological integrity. We demonstrated that the P-IBI can effectively reflect temporal and spatial variations of biological integrity and could be used as a potential tool to evaluate Lake Fuxian ecosystem health.

Iron bound phosphorus predominates the contribution of phosphorus to lake system from terrigenous source: The evidence from the small watershed scale.

The reduction of exogenous emissions of phosphorus (P) is a crucial measure for resolving eutrophication in lakes. However, the input of terrigenous materials still potentially contributes to an increase of P load in lake systems. In this study, we examined the phosphate oxygen isotope (δ18OP) of various P fractions in soils and sediments in a small lake watershed, namely, Shijiuhu watershed. The high-resolution in-situ diffusive gradients in thin films (DGT) technology was also used to survey the dynamic processes of P diffusion from sediment particles to the water. The results demonstrated that lighter δ18OP values (16.2-19.5‰) for individual P fractions in lake sediments were detected compared to other land-use patterns, indicating the cumulative biological P recycling on anaerobic condition. Fe bound P (Fe-P) overall had heavier δ18OP values (17.3-24.8‰) than some of Ca bound P (Ca-P) and equilibrium values, suggesting that Fe-P conserved the parental isotope signatures from terrigenous source and could act as the ideal tracer for the lake sediments. The mixing effect of terrigenous detrital input and biological mineralization made the source identification uncertain by using Ca-P, which had a wider range of δ18OP values (13.0-26.6‰). Additionally, significantly positive correlation (r = 0.551-0.913, p<0.05) between soluble reactive P (SRP) and Fe2+ in interstitial water obtained using DGT measurement revealed the conspicuous release and desorption of solid Fe-P toward the water. High diffusion fluxes from the sediments toward the overlying water further demonstrated that the desorption of Fe-P in the soil-originated sediments toward the solution conspicuously facilitated the accumulation of SRP in lake water. The first-time application of δ18OP isotope combined with in-situ DGT techniques certified that it's feasible for the contribution confirmation from terrigenous to lacustrine environments, and presented the direct evidence for management strategy making about P control and eutrophication restoration at the catchment scale of lakes.

Seasonal variation regulate the endogenous phosphorus release in sediments of Shijiuhu Lake via water-level fluctuation.

Freshwater lakes undergo substantial alterations of the phosphorus (P) cycle in the water-sediment ecosystem due to thermal change. The impact process of seasonal fluctuation on P cycling in sediments has been scarcely investigated. P forms in sediments from a freshwater lake in China were analyzed using sequential extraction technique. The vertical distribution of soluble reactive P (SRP), Fe2+, and S2- in the interstitial water was measured using diffusion gradient technique (DGT). Fick's Law and DIFS model were used to obtain the diffusion fluxes of SRP and the kinetic parameters in the water-sediment system. The results showed that total P (TP) concentrations in the solid sediments varied from 207.5, 266.6 and 130.3 mg/kg to 614.7, 1053.1, and 687.6 mg/kg in winter, spring, and summer, respectively. The concentrations of individual P forms in spring were higher than those in other seasons, with Fe-bound P (Fe-P) concentration being the highest across all seasons. Notably, significant variations of SRP concentrations were found in the interstitial water between sedimentary depths of approximately 2 cm and 6 cm, particularly in the summer. Furthermore, higher diffusion fluxes of SRP through the interface were found in summer. A stable anaerobic environment failed to develop in spring with high water level, preventing the desorption of solid Fe-P and diffusion of Fe2+ into the water due to the afflux and deposition of P-containing particulate into deeper sediment layers along with organic material. Under extreme high-temperature in summer, decreased rainfall and rising temperatures boosted the activity of aquatic organisms in the water, thereby reducing P fixation by sediments and leading to P release. This process increased the risk of P excess and potential eutrophication in the water. Generally, clarifying the resupplying processes of endogenous P in sediment systems experiencing seasonal variations is critical for eutrophication management of lakes.

Two-dimensional, high-resolution imaging of pH dynamics in the phyllosphere of submerged macrophyte using a new Nano-optode.

The phyllosphere pH helps shape the plant microbiome and strongly influences aboveground interactions in plant canopies. Yet little is known about the distribution of pH at a microscale within the macrophyte phyllosphere and the factors promoting them because achieving high-resolution quantitative imaging of phyllosphere pH is a great challenge. Here, new ratiometric pH nano-optodes were prepared by firstly encapsulating the self-synthesized lipophilic dyes (8-acetoxypyrene-N1, N3, N6-trioctadecyl-1, 3, 6-tri-trisulfonamide) to poly(1-vinylpyrrolidone-co-styrene) nanoparticles, and then immobilizing the resulting nanoparticles in polyurethane hydrogel on transparent foils. The nano-optodes presented reversible and fast response (t95 < 80 s) to the pH range from 7.0 to 11.0, with merits of good spatial resolution, photobleaching/leaching resistance and negligible cross-sensitives toward temperature, O2 and ionic strength (< 100 mM). The nano-optodes together with a self-designed phyllosphere chamber were further applied to directly measure the pH distributions at a microscale around single leaves of V. spiralis grown in natural sediment. The pronounced pH microheterogeneity and leaf basification within the V. spiralis phyllosphere were quantitatively visualized. We also provided direct empirical evidence that the dynamic of the phyllosphere pH at high resolution was significantly controlled by the shifting light intensity and temperature. Implementation of the nano-optodes holds great potential for various laboratory applications, which will provide an in-depth insight into phyllosphere activities on the microscale.

How phoD-harboring functional microbial populations trigger the release risk of phosphorus in water sediment system of Shijiuhu Lake, China after experiencing the transseasonal shift.

Excessive phosphorus (P) enrichment is the critical cause of eutrophication in the lake water. Organic P (Po) mineralization processes induced by alkaline phosphatase (APase) regulated by phoD-encoding microorganisms in the lake ecosystems was still ambiguous due to the transseasonal shift of water temperatures and depths. Different P pools in the water and sediments of Shijiuhu Lake at varied seasons were measured using chemical extraction methods and solution 31P NMR. The alkaline phosphatase activity (APA) in the sediments were assessed together with enzyme kinetic parameters. The abundances and compositions of microbial communities encoding functional gene phoD were also obtained using high-throughput sequencing. The results showed that Po concentrations remarkably increased from winter toward spring when having higher water depths due to the terrigenous input and biomass deposition. Noteworthy elevation in the PO43- concentration was observed in the interstitial water during the spring, particularly at around 5 cm sediment depth with value reaching as high as 0.43 mg/L. The degradation and mineralization of momoesters and diesters with higher concentrations in the sediments of spring aggravated the PO43- load in the interstitial water. Higher APA reaching 91.6 µg/(g·h) in spring was responsible for the mineralization of Po. Remarkably upwards increasing of absolute abundance of phoD-encoding gene in spring reaching up to 2.6 times of that in winter facilitated the generation of APA in spring. Cobetia and Calothrix followed by Aquabacterium and Mitsuaria were the most abundant phoD-encoding genera with relative abundance > 4%. Weakly positive correlation between dominant bacterial genera and APA and P fractions suggested that low-abundance genera was also involved in the APA generation and Po hydrolysis. These results indicate that spring with high water temperature and depth facilitate the mineralization of Po in the sediment and increase of labile PO43- load in the water, further provide valuable information for the management of eutrophic lakes.

Impact of seasonal change on dissimilatory nitrate reduction to ammonium (DNRA) triggering the retention of nitrogen in lake.

Nitrogen (N) reduction processes including denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are critical for the eutrophication in the lake water. However, the understanding about the dominant pathways of N cycling keep limited due to the high complexity of N cycle processes in lacustrine environment. The N fractions in sediments collected from Shijiuhu Lake were measured using high-resolution (HR)-Peeper technique and chemical extraction method in varied seasons. The abundance and microbial community compositions of functional genes involved in various N-cycling processes were also obtained using high-throughput sequencing. The results showed that NH4+ concentrations in the pore water remarkably increased from the upper layer toward the deeper layer and from winter to spring. This trend suggested that higher temperature facilitated the accumulation of NH4+ in the water. Decreased NO3- concentrations were also detected at deeper sediment layers and higher temperature, indicating the intensification of N reduction on anaerobic conditions. The NH4+-N concentrations reduced in spring along with the slight change of NO3--N in solid sediment, indicating the desorption and release of mobile NH4+ from solid phase to the solution. Remarkably decreased absolute abundances of functional genes were found in spring with DNRA bacteria nrfA gene as dominant genus and Anaeromyxobacter as the most dominant bacterium (21.67 ± 1.03%). Higher absolute abundance (146.2-788.1 × 105 Copies/g) of nrfA gene relative to other genes was mainly responsible for the increase of bio-available NH4+ in the sediments. Generally, microbial DNRA pathway predominated the N reduction and retention processes in the lake sediment at higher temperature and water depth even experiencing the suppression of DNRA bacteria abundance. These results suggested the existence of ecological risk via N retention by the action of the DNRA bacteria in the sediment on the condition of higher temperature, further provided valuable information for N management of eutrophic lakes.

Phosphorus Accumulation in Extracellular Polymeric Substances (EPS) of Colony-Forming Cyanobacteria Challenges Imbalanced Nutrient Reduction Strategies in Eutrophic Lakes.

Extracellular polymeric substances (EPS) are crucial for cyanobacterial proliferation; however, certain queries, including how EPS affects cellular nutrient processes and what are the implications for nutrient management in lakes, are not well documented. Here, the dynamics of cyanobacterial EPS-associated phosphorus (EPS-P) were examined both in a shallow eutrophic lake (Lake Taihu, China) and in laboratory experiments with respect to nitrogen (N) and phosphorus (P) availability. Results indicated that 40-65% of the total cyanobacterial aggregate/particulate P presented as EPS-P (mainly labile P and Fe/Al-P). Phosphorus-starved cyanobacteria rapidly replenished their EPS-P pools after the P was resupplied, and the P concentration in this pool was stable for long afterward, although the environmental P concentration decreased dramatically. A low-N treatment enhanced the EPS production alongside two-fold EPS-P accumulation (particularly labile P) higher than the control. Such patterns occurred in the lake where EPS and EPS-P contents were high under N limitation. EPS-P enrichment increased the P content in cyanobacteria; subsequently, it could hold the total P concentration higher for longer and make bloom mitigation harder. The findings outline a new insight into EPS functions in the P process of cyanobacterial aggregates and encourage consideration of both N and P reductions in nutrient management.

Nutrient enrichment drives the sediment microbial communities in Chinese mitten crab Eriocheir sinensis culture.

Microbial communities play a critical role in aquaculture ecosystems. To identify the influence of sediment nutrient levels on microbial communities, sediment and water samples were collected from Chinese mitten crab Eriocheir sinensis culture ponds with different nutrient enrichment levels. Relevant physicochemical properties were measured, and 16 S rRNA gene sequencing was applied to identify relevant bacterial communities in the sediments. The results showed that the diversity and composition of microbial communities in sediments with different levels of nutrient enrichment varied considerably. Proteobacteria was the most abundant phylum in all samples, followed by Bacteroidetes, and Desulfobacterota with relative abundances of 23.5-40.9%, 9.8-21.5%, and 9.6-18.1%, respectively. Notably, total nitrogen (TN), organic matter (OM), and pH were important factors driving sediment bacterial community aggregation, the TN concentration explaining 61.5% of the microbial community variation. This study highlights that long-term culture activities alter the degree of sediment nutrient enrichment, which in turn affects microbial community composition and may ultimately have an impact on culture efficiency.

Effects of nutrient reduction and habitat heterogeneity on benthic macroinvertebrate assemblages in a large shallow eutrophic lake.

The Taihu Lake ecosystem has been subjected to numerous anthropogenic stressors during the past decades, leading to substantial changes in nutrient dynamics and habitat quality. For instance, the northwestern lake bays receive large amounts of nutrient-rich wastewater and have frequently experienced algal blooms, while the eastern lake region is still dominated by submersed macrophytes. Such changes in environmental characteristics can greatly impact benthic macroinvertebrate communities. We used a 15-year monitoring data series collected by the Taihu Laboratory for Lake Ecosystem Research to examine the spatial and temporal variations of the benthic invertebrate fauna and evaluate its status and trends. We found that three major communities could be distinguished based on taxonomic group composition and abundance, and these corresponded well with three lake habitat types: algal-dominated, macrophyte-dominated, and open-lake zone. An analysis of temporal trends showed major changes in the macroinvertebrates during the study period, largely driven by a lake-wide and significant decline in the abundance of pollution-tolerant taxa. The spatial and temporal variations of macroinvertebrate communities were mainly explained by nutrients (e.g., total nitrogen and ammonium concentrations) and habitat factors (e.g., sediment substrates and macrophyte biomass) as indicated by Random Forests regression, but the major drivers of macroinvertebrate density differed among the three lake zones at the temporal scale. Moreover, our findings suggest that benthic invertebrates were more sensitive to the improvement of the lake's environmental conditions than the pelagic community was. This study provides insights into the responses of macroinvertebrates to ecological dynamics in lakes and highlights the importance of continued monitoring for tracking long-term changes.

Response of zooplankton to nutrient reduction and enhanced fish predation in a shallow eutrophic lake.

As a key link between top-down regulators and bottom-up factors, zooplankton responds sensitively to environmental variations and provides information on the ecological state of freshwater systems. Although the response of zooplankton to anthropogenic pressures and fluctuating natural conditions, such as nutrient loading and climate change, has been extensively examined, findings have varied markedly. The mechanistic basis for the correlation between environmental variability and the zooplankton community is still debated, particularly for subtropical eutrophic lakes. We used two methods to analyze physicochemical and selected biological variables derived from long-term monitoring of Lake Taihu, a subtropical shallow lake in China. We first applied random forest regression to examine how changes in zooplankton were related to a set of environmental variables on interannual time scales. Then we used the results to guide the construction of a conceptual model for piecewise structural equation modeling (pSEM) to quantify more precisely the zooplankton-environment relationship. Zooplanktivorous fish and nutrient concentrations were the most important predictors of long-term trends in zooplankton in RF regression. Intensification of planktivorous fish predation led to a lower zooplankton biomass and smaller individuals through the removal of larger crustaceans. Moreover, suppression of zooplankton can in part be explained by increases in inedible algae, triggered by a combination of reduced nutrient concentrations and weakened grazer control. These results were also confirmed in the pSEM, which further indicated that top-down regulators might be more important than bottom-up factors for the zooplankton community in Lake Taihu. Our results suggest that stocking of filter-feeding fish in the lake did not meet the expectation that they would control algae, but that the use of biomanipulation measures considering both water quality and fishery management seems promising. This study offers insights into how indicator metrics of zooplankton can improve our understanding of the associations between plankton communities and ecosystem alterations.

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Microcystis aeruginosa is a common bloom-forming cyanobacterium, which generally coexists and competes with Chlorella pyrenoidosa in lakes. Sonication can be used for emergency management of algal blooms. Ultrasound influences algal growth and physiological parameters, as well as interspecific competition in algal community. To explore the effects of ultrasonic stress (35 kHz, 0.035 W·cm-3) on physiological characteristics and interspecific competition of algae, M. aeruginosa and C. pyrenoidosa were sonicated in mono- and co-cultures (1:1 mixture, according to cell concentration). Results showed that M. aeruginosa was more sensitive to ultrasonic stress. After the sonication for 600 s, both photosynthetic activity (Fv/Fm) and esterase activity of M. aeruginosa showed significant changes, with Fv/Fm values in mono- and co-cultures being decreased by 51.8% and 64.7%, respectively. In comparison, Fv/Fm values of C. pyrenoidosa changed slightly. M. aeruginosa released more chromophoric dissolved organic matter (CDOM, including tryptophan-, tyrosine-, and fulvic-like substances) than C. pyrenoidosa. The cell concentration of C. pyrenoidosa showed little changes regardless of sonication time, while the cell concentration of M. aeruginosa decreased at different degrees. The cell concentration of M. aeruginosa in co-cultures decreased by 42.6% after sonication for 600 s, which might be responsible for the dominance of C. pyrenoidosa during 8 days after sonication. M. aeruginosa inhibited C. pyrenoidosa in other treatments, but mutual inhibition appeared in the 600 s sonication treatment. After ultrasonic treatment, the activity of M. aeruginosa could recover gradually. The treatment should be conducted again within a week to improve the persistence of algal control.

Transcriptome profiling reveals toxicity mechanisms following sertraline exposure in the brain of juvenile zebrafish (Danio rerio).

Sertraline (SER) is one of the most commonly detected antidepressants in the aquatic environment that can negatively affect aquatic organisms at low concentrations. Despite some knowledge on its acute toxicity to fish, the effects of chronic SER exposure remain poorly understood along with any underlying mechanisms of SER-induced toxicity. To address this knowledge gap, the effects of chronic exposure to three SER concentrations from low to high were investigated in zebrafish. Juvenile zebrafish were exposed to three concentrations of 1, 10, or 100 µg/L of SER for 28 d, after which indicators of oxidative stress and neurotoxicity in the brain were measured. Superoxide dismutase (SOD) activity was significantly enhanced by SER at 1 up to 100 µg/L, and catalase (CAT) activity was significantly induced by SER at 1 or 10 µg/L. The activity of acetylcholinesterase (AChE) was significantly induced by 10 and 100 µg/L of SER, and the serotonin (5-HT) level was significantly increased by all three concentrations of SER. To ascertain mechanisms of SER-induced toxicity, transcriptomics was conducted in the brain of zebrafish following 100 µg/L SER exposure. The molecular signaling pathways connected with circadian system and the immune system were significantly altered in the zebrafish brain. Based on transcriptomic data, the expression levels of six circadian clock genes were measured, and three genes were significantly altered in relative abundance in fish from all experimental treatments with SER, including cryptochrome circadian regulator 2 (cry2), period circadian clock 2 (per2), and period circadian clock 3 (per3). We hypothesize that the circadian system may be related to SER-induced neurotoxicity and oxidative stress in the central nervous system. This study reveals potential mechanisms and key events (i.e., oxidative stress and neurotoxicity) associated with SER-induced toxicity, and improves understanding of the molecular and biochemical pathways putatively perturbed by SER.

Comparison of acid volatile sulphide, metal speciation, and diffusive gradients in thin-film measurement for metal toxicity assessment of sediments in Lake Chaohu, China.

The toxicity of heavy metals in sediments is inseparable from their forms in the environment. Traditional sediment toxicity assessment systems, such as total metals, dissolved metals in pore water, metals extracted by the Community Bureau of Reference procedure, and acid volatile sulphide (AVS)-simultaneously extracted metal (SEM), have their own limitations. This study revealed the horizontal and vertical distribution characteristics of AVS and SEM in Lake Chaohu and three typical groups of two-dimensional profiles of diffusive gradients in thin-film (DGT)-labile S(-II) were obtained at representative sampling sites. There was a positive correlation between DGT-labile S(-II) and AVS due to sulphate-reducing bacteria and a negative correlation due to the high sulphate reduction rate induced by high total organic carbon. Moreover, there was no correlation between DGT-labile S(-II) and AVS when bioturbation was dominant in the sediments. To realise the application of DGT measurement in toxicity assessment of heavy metals in sediment through the sandwich relationship of DGT-labile metals vs. metals speciation vs. sediment toxicity assessment, the key relationship of DGT-labile metals vs. metals speciation was explored. DGT-labile Ni showed potential to reveal this relationship.

Dissimilatory nitrate reduction to ammonium (DNRA) potentially facilitates the accumulation of phosphorus in lake water from sediment.

Nitrogen (N) and phosphorus (P) are crucial nutrients for eutrophication in the lacustrine ecosystem and attract the attention worldwide. However, the interaction between them need further clarification. This study aimed to assess the influence of dissimilatory nitrate reduction to ammonia (DNRA) on the cycle of P in lacustrine sediment. Different fractions of N and P in the pore water were measured using high-resolution in-situ measurement techniques, HR-Peeper and DGT, coupling with sequential extraction for solid sediment from a shallow freshwater lake. The results showed that elevated nitrate (NO3-) reduction via DNRA rather than denitrification was verified at deeper sediment layer, suggesting the generation of inorganic ammonia (NH4+) as electron donor under anaerobic episodes. High abundance of DNRA bacteria (nrfA gene) obtained using high-throughput sequencing analysis were detected at upper layer and responsible for the accumulation of NH4+ in the sediment coupling with chemolithoautotrophic metabolism. Additionally, significant desorption of ionic ferrous iron (Fe2+) and dissolved reactive phosphate (DRP) from solid phase and the enrichment in the solution was simultaneously detected. Higher concentration of solid Fe bound P (Fe-P) at deeper layer indicated the potential re-oxidation of Fe2+ as electron donor during DNRA process and sorption of DRP toward the Fe-containing minerals. However, obvious evidence of desorption proved by DGT indicated that higher NH4+ concentrations favored the reduction of Fe(III) oxy(hydr)oxides and the desorption of DRP into the pore water and diffusion toward the overlying water. Finally, noteworthy S2- release from solid sediment was speculated to stimulate the DNRA and facilitated the accumulation of NH4+ in the solution, which further induced the enrichment of DRP in water from the solid phase. Overall, DNRA potentially facilitates the accumulation of P in lake water, and the synchronous control of N and P is important for the eutrophication management and restoration of lake eutrophication.