[Modulation effect of Acanthopanax senticosus polysaccharides through inflammatory cytokines in protecting immunological liver-injured mice].

The aim of this paper was to discuss the protective effect and mechanism of Acanthopanax senticosus polysaccharides( ASPs) on immunological liver injury caused by conanavalin A( Con A). BALB/c mice were randomly divided into seven groups: control group,model group( Con A),low-,medium-,and high-dose( 36. 25,72. 5,145 mg·kg~(-1)) ASPs groups,bifendate( 200 mg·kg~(-1),positive drug) group and pyrrolidinedithiocarbamate( PDTC,NF-κB inhibitor,200 mg·kg~(-1)) group. ASPs groups and bifendate group were given with corresponding drugs by ig administration once daily for 7 d. Control group,model group and PDTC group were given with normal saline by ig administration once daily for 7 d. After the last ig administration,PDTC was given in DTC group by iv administration( 200 mg·kg~(-1)); 0. 5 h after that,Con A( 20 mg·kg~(-1)) was injected via the tail vein to induce immunological liver injury in all the mice except normal control group. The mice were killed 8 h later and their liver tissues were collected for histopathological examination. The contents of nitric oxide( NO),superoxide dismutase( SOD),malondialdehyde( MDA),reduced glutathione( GSHPX),interleukin( IL-1ß) and tumor necrosis factor( TNF-α) in liver tissues were detected by kit assay. Western blot method was used to detect TNF-α,intercellular cell adhesion molecule-1( ICAM-1),inducible nitric oxide synthase( i NOS) and nuclear factor( NF-κB) protein expression in liver tissues. As compared with model group,ASPs not only could reduce the activity of MDA,NO,IL-1ß and TNF-α,but also increase the content of GSH-PX and SOD; at the same time,the protein expression levels of TNF-α,ICAM-1,i NOS and NF-κB were reduced in liver tissues; in addition,inflammatory cell infiltration was alleviated,hepatocyte cytoplasm was loose and swollen,and nuclear condensation and staining were improved. ASPs has a protective effect on immunological liver injury,and the mechanism may be associated with regulating secretion of inflammatory cytokines and the expression of adhesion factor through NF-κB signaling pathway.

Facile Synthesis and High Activity of Novel Composite C/Fe-BiVO4 Photocatalyst for Degradation of Cipfloxacin.

A novel C/Fe-BiVO4 nanosheet composite photocatalyst combining the properties of both semiconductor and Fenton like catalyst was prepared via a two-step method involving Resin carbonization and hydrothermal process. The samples were characterized by X-ray diffraction (XRD), scanning electronic microscopy (SEM) and energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), UV-vis diffuse reflectance spectra (DRS), X-ray photoelectron spectroscopy (XPS), Fourier transformed infrared spectroscopy (FT-IR) and nitrogen adsorption-desorption measurements and fluorescence spectrum technique. Structure analyses indicated that C/Fe-BiVO4 presented a nanosheet and macro-meso dual porosity structure. Photocatalytic degradation of cipfloxacin (CIP) (10 mg/L) was studied using C/Fe-BiVO4 under simulated solar light (SSL) irradiation in a cylindrical reactor. Three experimental parameters were chosen as independent variables: pH, C/Fe-BiVO4 concentration, and H2O2 concentration. The catalyst shows high catalytic activity in cipfloxacin photodegradation reaction with the cipfloxacin conversion efficiency higher than 95.61% at experimental conditions (catalyst dose, 0.75 g · L-1; H2O2 dose, 200 mg · L-1; solution pH, 5.0). The process obeyed the pseudo first-order kinetics by assuming a constant concentration of OH•. The as-prepared composite exhibited high efficiency in the photocatalytic decomposition of Cipfloxacin (CIP) by the assistance of H2O2. This method is promising due to its inexpensive starting materials and good photocatalyst for degradation of emerging micropollutants.