A Florfenicol-Resistant Plasmid Shuttling Between Actinobacillus pleuropneumoniae and Glaesserella parasuis.

Porcine contagious pleuropneumonia, caused by Actinobacillus pleuropneumoniae, has resulted in significant economic losses to the swine industry. Although antibiotics are commonly employed to control this disease, their widespread use or misuse can lead to the development of antibiotic resistance in A. pleuropneumoniae. Consequently, it is crucial to conduct antimicrobial susceptibility testing on clinical isolates. In our study, we identified one strain of A. pleuropneumoniae with resistance to florfenicol and extracted a 5919 bp plasmid named pAPPJY, which confers florfenicol resistance. Sequence analysis revealed that the plasmid contains four open reading frames, namely rep, antioxin vbha family protein, floR, and a partial copy of lysr. Although a few variations in gene position were observed, the plasmid sequence exhibits a high degree of similarity to other florfenicol-resistant plasmids found in Glaesserella parasuis and A. pleuropneumoniae. Therefore, it is possible that the pAPPJY plasmid functions as a shuttle, facilitating the spread of florfenicol resistance between G. parasuis and A. pleuropneumoniae. In addition, partial recombination may occur during bacterial propagation. In conclusion, this study highlights the horizontal transmission of antibiotic resistance among different bacterial species through plasmids, underscoring the need for increased attention to antibiotic usage.

Discovery of a Novel Integrative Conjugative Element ICEAplChn2 Related to SXT/R391 in Actinobacillus pleuropneumoniae.

Objective: The objective of this study was to characterize ICEAplChn2, a novel SXT/R391-related integration and conjugation element (ICE) carrying 19 drug resistance genes, in a clinical isolate of Actinobacillus pleuropneumoniae from swine. Methods: Whole genome sequencing (WGS) of A. pleuropneumoniae CP063424 strain was completed using a combination of third-generation PacBio and second-generation Illumina. The putative ICE was predicted by the online tool ICEfinder. ICEAplChn2 was analyzed by PCR, conjugation experiments, and bioinformatics tools. Results: A. pleuropneumoniae CP063424 strain exhibited high minimum inhibitory concentrations of clindamycin (1,024 mg/L). The WGS data revealed that ICEAplChn2, with a length of 167,870 bp and encoding 151 genes, including multiple antibiotic resistance genes such as erm(42), VanE, LpxC, dfrA1, golS, aadA3, EreA, dfrA32, tetR(C), tet(C), sul2, aph(3)″-lb, aph(6)-l, floR, dfrA, ANT(3″)-IIa, catB11, and VanRE, was found to be related to the SXT/R391 family on the chromosome of A. pleuronipneumoniae CP063424. The circular intermediate of ICEAplChn2 was detected by PCR, but conjugation experiments showed that it was not self-transmissible. Conclusions: To our knowledge, ICEAplChn2 is the longest member with the most resistance genes in the SXT/R391 family. Meanwhile, ATP-binding cassette superfamily was found to be inserted in the ICEAplChn2 and possessed a new insertion region, which is the first description in the SXT/R391 family.

Pili Subunit PilA Contributes to the Cytoadhesion of Glaesserella Parasuis to Host Cells and Provides Immunoprotection.

Glaesserella parasuis (G. parasuis) is commonly located in the upper respiratory tract of pigs as an opportunistic pathogen. It can cause Glässer's disease, which leads to serious economic losses in the swine industry. The occurrence of the disease is often linked with the adhesion and colonization of the pathogen. The PilA pilus subunit is important for adhesion to the host, twitching motility, and biofilm formation in many bacteria. However, no research has focused on the function of PilA in G. parasuis. To further reveal the pathogenesis of G. parasuis and to search for subunit vaccine candidates, we investigated whether PilA could adhere to cells and provide immune protection. A bioinformatic analysis showed that the protein secondary structure of the G. parasuis PilA was similar to that of Haemophilus influenzae (HI). Cell adhesion, ELISA, and far-Western blotting showed that rPilA could bind porcine-derived, porcine kidney-15 (PK-15) cells, swine tracheal epithelial cells (STECs), and the extracellular matrix components fibronectin (FN) and laminin (LN). An immunogenicity analysis showed that recombinant PilA (rPilA) reacted specifically with convalescent and hyperimmune serum. Importantly, purified rPilA elicited a strong immune response and conferred robust protection against challenges with serovar 5 G. parasuis in mice. These results suggested that the PilA protein might help G. parasuis adhere to host cells by binding to FN and LN, and its immunogenicity establishes it as a promising, novel subunit vaccine candidate against infections with G. parasuis. IMPORTANCE G. parasuis is one of the most prevalent bacterial infections in swine production and can lead to huge economic losses around the world. A full understanding of colonization and immunity with G. parasuis infections will be essential in disease control. In this study, the PilA protein, which is a common virulence factor in other bacteria that mediates adherence to the host, was assessed. The results suggested that the PilA protein of G. parasuis can mediate adhesion to host cells through FN and LN, which provides a new idea for the study of the pathogenicity of G. parasuis. Furthermore, fimbriae usually have high immunogenicity. Immunogenicity and protective capacity results showed that the use of this recombinant PilA antigen might be a promising candidate vaccine antigen with which to prevent G. parasuis infections.

Virus-host interaction networks as new antiviral drug targets for IAV and SARS-CoV-2.

Currently, SARS-CoV-2, especially the Omicron strain, is ravaging the world and even co-infecting human beings with IAV, which is a serious threat to human public health. As of yet, no specific antiviral drug has been discovered for SARS-CoV-2. This requires deeper understandings of the molecular mechanisms of SARS-CoV-2-host interaction, to explore antiviral drug targets and provide theoretical basis for developing anti-SARS-CoV-2 drugs. This article discussed IAV, which has been comprehensively studied and is expected to provide the most important reference value for the SARS-CoV-2 study apart from members of the Coronaviridae family. We wish to establish a theoretical system for the studies on virus-host interaction. Previous studies have shown that host PRRs recognize RNAs of IAV or SARS-CoV-2 and then activate innate immune signaling pathways to induce the expression of host restriction factors, such as ISGs, to ultimately inhibit viral replication. Meanwhile, viruses have also evolved various regulatory mechanisms to antagonize host innate immunity at transcriptional, translational, post-translational modification, and epigenetic levels. Besides, viruses can hijack supportive host factors for their replication. Notably, the race between host antiviral innate immunity and viral antagonism of host innate immunity forms virus-host interaction networks. Additionally, the viral replication cycle is co-regulated by proteins, ncRNAs, sugars, lipids, hormones, and inorganic salts. Given this, we updated the mappings of antiviral drug targets based on virus-host interaction networks and proposed an innovative idea that virus-host interaction networks as new antiviral drug targets for IAV and SARS-CoV-2 from the perspectives of viral immunology and systems biology.

The Influence of "Artificial Intelligence + Human-Computer Interaction" on Teachers' Psychological Changes in Academic Management in Colleges.

The purpose was to analyze the psychological changes of teaching staff in the academic management of local colleges, and briefly explore the role of teaching staff in the development of the social economy and colleges. In the environment of artificial intelligence and human-computer interaction (HCI), first, the relevant theories of teaching staffs' psychological status and the characteristics of teaching staff in college academic management were analyzed and expounded. Next, the way of the questionnaire was selected to analyze the psychology of teaching staff in college academic management at different ages, professional titles, academic qualifications, disciplines, and teaching years. The results showed that the mental health level of college teachers was lower than the current national adult standard; the mental health level of female teachers in colleges was higher than that of male teachers; the p value of mental health of college teachers with different ages, professional titles, education, disciplines, and teaching years was greater than 0.05, indicating that there was no significant difference; the p-value of professional academic and mental health was less than 0.01, indicating that there was a significant correlation, that was, teachers' professional academic exerted a significant impact on teachers' mental health. In short, under the background of artificial intelligence and HCI's rapid development, higher education was moving forward with high quality, and more attention should be paid to the psychological changes of college teaching staff.

Innovation Strategy of Cultivating Innovative Enterprise Talents for Young Entrepreneurs Under Higher Education.

A time series model is designed based on the backpropagation neural network to further optimize the innovation and development of new ventures. The specific situation of two factors is primarily analyzed as follows: the supply and demand ratio of enterprise talents and the state of entrepreneurship in the development of new ventures. The results show that the potential demand of future enterprises for big data talents can be obtained by fitting prediction sequences. Based on the Backpropagation-Autoregressive Integrated Moving Average model, the post modeling and prediction are carried out, and the coefficient 0.6235 is obtained by substituting the equation of Pearson's correlation coefficient. The analysis results suggest that the matching needs to be strengthened between the cultivation of innovative talents in universities and the demand trend of big data-related positions in enterprises. Moreover, there is a mismatch between the cultivation of innovative talents and the demand for innovative talents. Meanwhile, the mental health level of young entrepreneurs is concerned. The mental health status of young entrepreneurs is compared with the national norm data through the questionnaire survey and statistical data analysis. The results reveal that the mental health level of young entrepreneurs is significantly lower than that of the national norm, and the proportion of anxiety and depression is 29.4% and 27.5%, respectively. Considering the professional characteristics and competitive environment of young entrepreneurs, busy work and the multiple missions given by society to entrepreneurs are the major reasons for their pressure, and mental health problems are serious.

Construction of Innovation Behavior of College-Student Entrepreneurs Using Entrepreneurship and Innovation Theory Under Educational Psychology.

The purpose is to analyze the entrepreneurship and innovation education of colleges from the perspective of educational psychology and optimize the teaching mode reform of entrepreneurship and innovation courses. In this study, the theoretical research and case studies are combined to explore the performance of college-student entrepreneurs during college and work and have provided data for targeted entrepreneurship and innovation education in the schools. Meanwhile, the specific manifestation of the entrepreneurial spirit during work is analyzed, and the impact of entrepreneurial spirit is discussed on the new venture performance. The case study shows that most of the surveyed college-student entrepreneurs have a higher educational background and short venture-creation time, and their ventures are mostly small and medium-sized enterprises (SMEs) with rapid development. Most entrepreneurs show a strong entrepreneurial spirit during college. Among them, the average score of honesty is 3.85. At work, the surveyed entrepreneurs have high innovation attitude and innovation intention. Most entrepreneurs use innovative methods to solve practical problems in their work, and innovation spirit plays an important role in improving venture performance. Innovation attitude and innovation performance have a significant positive impact on innovation behavior. The research is comprehensive, and the results have very important application value. The results can provide scientific and effective references for colleges to reform entrepreneurship and innovation education.

Analysis on Influences of College Students' Psychological Capital in Entrepreneurial Learning Engagement.

College students' entrepreneurial learning engagement (ELE) is a key link that affects the success of future entrepreneurship. To analyze the influencing factors of the psychological capital (PC) dimension in college students' ELE, a total of 211 college students were selected from colleges in the Ningbo area for questionnaire survey. The principal component analysis method was used to test the difference validity of PC and ELE. One-way analysis of variance was used to analyze differences in demographic variables between PC, ELE, and positive emotions (PEs). Besides, the structural equation model was used to analyze the mediating role of PEs in PC and ELE. In addition, there was a significant difference between the unrestricted model of PC-ELE and the restricted model (p < 0.05), and the difference between the potential dimensions of "mental capital-learning input" was generally satisfactory; there were significant differences at the professional level (p < 0.01); PC had significant differences in family economic status (p < 0.01); the indirect path coefficients of PE added to the relationship between PC and ELE were 0.106 and 0.211, respectively, and there was no significance (p > 0.05). In short, the PC of college students has a significant positive influence (PI) on ELE, PC differs significantly in family economic status, and PEs differ significantly at the professional level. The research results show that there is no mediating effect of PEs in the relationship between PC and college students' ELE.

Zinc Finger CCCH-Type Antiviral Protein 1 Restricts the Viral Replication by Positively Regulating Type I Interferon Response.

Zinc finger CCCH-type antiviral protein 1 (ZC3HAV1) is a host antiviral factor that can repress translation and promote degradation of specific viral mRNAs. In this study, we found that expression of ZC3HAV1 was significantly induced by infection with influenza A virus (IAV) and Sendai virus (Sev). It was shown that deficiency of IFNAR resulted in a dramatic decrease in the virus-induced expression of ZC3HAV1. Furthermore, transfection with poly(I:C) and treatment with interferon ß (IFN-ß) induced the ZC3HAV1 expression. Interference with the endogenous expression of ZC3HAV1 enhanced the replication of influenza virus by impairing the production of IFN-ß and MxA, following the infection of influenza virus. In contrast, ectopic expression of ZC3HAV1 significantly restricted the replication of influenza virus by increasing the IFN-ß expression. In addition, ZC3HAV1 also promoted the induction of tumor necrosis factor and interleukin 6. These results suggest that ZC3HAV1 is induced by IFN-ß/IFNAR signaling during IAV and Sev infection and involved in positive regulation of IFN-dependent innate antiviral response.

Functional Involvement of Interferon-Inducible Transmembrane Proteins in Antiviral Immunity.

Interferons (IFNs) play crucial roles in host defense against viral infections by inducing the expression of numerous IFN-stimulated genes (ISGs) that can activate host antiviral immunity. Interferon-inducible transmembrane proteins (IFITMs), a family of small transmembrane proteins, are critical ISG products. Compelling evidence has implicated that IFITMs can establish an innate immune state to eliminate pathogens efficiently. IFITM proteins can impede broad-spectrum viral infection through various mechanisms. It is generally believed that IFITMs can block the viral entry by suppressing viral membrane fusion. However, some findings indicated that IFITMs might also inhibit viral gene expression and viral protein synthesis and thereby impair viral replication. IFITMs may incorporate into virions during viral assembly and thus reduce the infectivity of nascent virions. The precise inhibitory mechanism of IFITMs on viral infection and replication still requires further exploration. In this review, we highlight the recent findings regarding critical roles of IFITMs in host-virus interaction. We also discuss the molecular mechanisms underlying their functions in antiviral responses.