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1.
Opt Express ; 32(8): 13095-13110, 2024 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-38859288

RESUMO

Modulation format recognition (MFR) is a key technology for adaptive optical systems, but it faces significant challenges in underwater visible light communication (UVLC) due to the complex channel environment. Recent advances in deep learning have enabled remarkable achievements in image recognition, owing to the powerful feature extraction of neural networks (NN). However, the high computational complexity of NN limits their practicality in UVLC systems. This paper proposes a communication-informed knowledge distillation (CIKD) method that achieves high-precision and low-latency MFR with an ultra-lightweight student model. The student model consists of only one linear dense layer under a communication-informed auxiliary system and is trained under the guidance of a high-complexity and high-precision teacher model. The MFR task involves eight modulation formats: PAM4, QPSK, 8QAM-CIR, 8QAM-DIA, 16QAM, 16APSK, 32QAM, and 32APSK. Experimental results show that the student model based on CIKD can achieve comparable accuracy to the teacher model. After knowledge transfer, the prediction accuracy of the student model can be increased by up to 87%. Besides, it is worth noting that CIKD's inference accuracy can reach up to 100%. Moreover, the parameters constituting the student model in CIKD correspond to merely 18% of the parameters found in the teacher model, which facilitates the hardware deployment and online data processing of MFR algorithms in UVLC systems.

3.
Sci Rep ; 14(1): 11368, 2024 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-38762528

RESUMO

High-speed GaN-based lasers play a pivotal role in visible light communication (VLC) systems; however, the causes of the limited modulation response of our fabricated laser diode (LD) are not fully understood. Accordingly, we constructed an equivalent circuit model for both the LD and its packaging. This model enabled us to analyze the series resistance and parallel capacitance of the LD at different injection currents. Experiments and simulations were performed to investigate the intrinsic responses of the LD. The series resistance and parallel capacitance are responsible for S21 roll-off at low frequencies. Determination of the packaging design parameters on the modulation response of a transistor outline (TO)-can packaged LD was investigated which is important to achieve the impedance match in the future. The value of each discrete component was determined by fitting the scattering parameters of the equivalent circuit model to the packaged LD. Reducing the series resistance and parallel capacitance improved the modulation response. Our study firstly illustrates the design and manufacture of violet-blue-green laser transmitters with a large modulation bandwidth for ultra-high-speed VLC from the point of the impedance influence.

4.
Opt Lett ; 49(10): 2805-2808, 2024 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-38748166

RESUMO

The advancement demands of high-speed wireless data link ask for higher requirements on visible light communication (VLC), where wide coverage stands as a critical criterion. Here, we present the design and implementation of a transmitter structure capable of emitting a high-power wide-coverage white light laser. This laser source exhibits excellent stability, with an irradiation range extending to a half-angle of 20°. Its high brightness satisfies the needs of indoor illumination while maintaining excellent communication performance. Utilizing bit-loading discrete multi-tone modulation, a peak data transmission rate of 3.24 Gbps has been achieved, spanning 1 to 5 m. Remarkably, the data rates exceed 2.5 Gbps within a 40° range at a distance of 5 m, enabling a long-distance, wide coverage, high-speed VLC link for future mobile network applications.

5.
Water Res ; 258: 121767, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38754296

RESUMO

The co-occurrence of high As and F concentrations in saline groundwater in arid and semi-arid regions has attracted considerable attention. However, the factors determining the elevated concentrations of the two elements in surface water in these regions have not been sufficiently studied, and their implications for the poor-quality of local groundwater (high levels of As, F, and salinity) are unknown. A total of 18 water samples were collected from Wuliangsu Lake, irrigation/drainage channels, and the Huanghe (i.e., Yellow River) in the Hetao Basin, China. The pH, concentrations of As and F as well as those of other major elements, and stable isotope (H and O) compositions were analyzed. The water samples had a high pH (7.85-9.01, mean 8.25) and high TDS (402-9778 mg/L, mean 1920 mg/L) values. In six of the 10 lake samples, As concentration was above 10 µg/L (maximum 69.1 µg/L) and, in one of them, F concentration was above 1.5 mg/L. Interestingly, the high As, F, and TDS values simultaneously detected in the lake water were similar to those previously reported in local groundwater, and all water samples showed a significant positive correlation between As and F concentrations (R2 = 0.96, p < 0.01), except for two samples with abnormally high Ca2+ levels. The results of stable isotope analysis and Cl/Br ratios suggested that the lake experienced strong evaporation, which is consistent with the high TDS values. Evaporative concentration is suggested as the main factor contributing to the elevated As and F concentrations in the lake water. In addition, the major ions (e.g., Na+, Cl-, HCO3-, and OH-) and pH in the lake water increased during evaporation, leading to desorption of As and F. Thus, the evaporation process, including evaporative concentration and desorption, was considered primarily responsible for the elevated As and F in the lake water. Based on the results of this study, we presume that the paleolakes in the study area have experienced intense evaporation process. As a result, As, F, and major elements accumulated in sediments (or residual lake water) and were buried in the fluvial basins; then, they were released into the groundwater through multiple (bio)hydrogeochemical processes. By combining the results of this study with those obtained from previous groundwater analyses, we propose a new hypothesis explaining the origin of elevated As and F concentrations in saline groundwater in arid and semi-arid regions.


Assuntos
Arsênio , Fluoretos , Água Subterrânea , Lagos , Poluentes Químicos da Água , Água Subterrânea/química , Lagos/química , China , Arsênio/análise , Poluentes Químicos da Água/análise , Fluoretos/análise , Monitoramento Ambiental , Rios/química , Concentração de Íons de Hidrogênio
6.
Sensors (Basel) ; 24(10)2024 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-38793940

RESUMO

Mobile visible light communication (VLC) is key for integrating lighting and communication applications in the 6G era, yet there exists a notable gap in experimental research on mobile VLC. In this study, we introduce a mobile VLC system and investigate the impact of mobility speed on communication performance. Leveraging a laser-based light transmitter with a wide coverage, we enable a light fidelity (LiFi) system with a mobile receiving end. The system is capable of supporting distances from 1 m to 4 m without a lens and could maintain a transmission rate of 500 Mbps. The transmission is stable at distances of 1 m and 2 m, but an increase in distance and speed introduces interference to the system, leading to a rise in the Bit Error Rate (BER). The mobile VLC experimental system provides a viable solution to the issue of mobile access in the integration of lighting and communication applications, establishing a solid practical foundation for future research.

7.
Opt Lett ; 49(7): 1656-1659, 2024 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-38560829

RESUMO

The escalating surge in datacenter traffic creates a pressing demand for augmenting the capacity of cost-effective intensity modulation and direct detection (IM/DD) systems. In this Letter, we report the demonstration of the single-lane 128-GBaud probabilistically shaped (PS)-PAM-20 IM/DD transmission using only a single digital-to-analog converter (DAC) for a net 400 G/λ system. Based on the advanced digital signal processing (DSP), we achieve net bitrates of up to 437 Gb/s for optical back-to-back and 432 Gb/s after the 0.5-km SSMF transmission in the C-band with 128-Gbaud PS-PAM-20 signals. This work is the latest demonstration on ultra-high-order PS-PAM signals achieving net bitrates exceeding 400 Gb/s despite symbol rate limitations. Notably, to the best of our knowledge, the realized net information rate ([net bitrate]/[symbol rate]) of 3.37 marks a new achievement within the domain of 400 G/λ IM/DD systems, with promising implications for enhancing bandwidth efficiency in the upcoming 1.6-Tb Ethernet scenario.

8.
Polymers (Basel) ; 16(8)2024 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-38674952

RESUMO

As one of the most widely applied general-purpose plastics, high-density polyethylene (HDPE) exhibits good comprehensive performance. However, mechanical strength limits its wider application. In this work, we introduced the engineering plastic PA6 as a dispersed phase to modify the HDPE matrix and applied multiple shears generated by vibration to the polymer melt during the packing stage of injection molding. SEM, 2D-WXRD and 2D-SAXS were used to characterize the morphology and structure of the samples. The results show that under the effect of a strong shear field, the dispersed phase in the composites can form in situ microfibers and numerous high-strength shish-kebab and hybrid shish-kebab structures are formed. Additionally, the distribution of fibers and high-strength oriented structures in the composites expands to the core region with the increase in vibration times. As a result, the tensile strength, tensile modulus and surface hardness of VIM-6 can reach a high level of 66.5 MPa, 981.4 MPa and 72, respectively. Therefore, a high-performance HDPE product is successfully prepared in this study, which is of great importance for expanding the application range of HDPE products.

9.
Ann Cardiothorac Surg ; 13(2): 165-172, 2024 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-38590995

RESUMO

Background: Thoracoscopic ablation (TA) has emerged as a promising treatment for atrial fibrillation (AF), with the Cox-Maze IV Procedure (CMP-IV) as the current gold-standard intervention. This study aims to evaluate and compare the outcomes of TA and CMP-IV in treating AF. Methods: Patients with AF underwent either CMP-IV or TA through a left-side chest approach. The CMP-IV entailed bi-atrium ablation, whereas the TA involved creating three circular plus three linear ablations in the left atrium. We analyzed baseline characteristics, perioperative outcomes and recurrence rates using propensity score matching (PSM) at a 1:1 ratio, to ensure comparability between the two treatment groups. Results: A total of 459 patients underwent either CMP-IV (n=93) or TA via left chest (n=366) and 174 patients were deemed eligible for 1:1 PSM. The TA group experienced significantly shorter intensive care unit (ICU) and hospital stays. The mean follow-up period was 31.5±22.1 months. Pre- and post-matching analysis showed that CMP-IV had a higher rate of freedom from recurrence compared to TA, particularly in non-paroxysmal AF patients. Multivariable Cox regression analysis revealed that CMP-IV was associated with a reduced risk of recurrence, while an increased left atrial size emerged as an independent predictor of postoperative recurrence, regardless of the use of CMP-IV or TA. Conclusions: Our study suggests that while the therapeutic efficacy of TA for "lone" AF may fall short of the classic CMP-IV, its less invasive nature results in significantly shorter ICU and hospital stays. To enhance patient outcomes following TA, it is essential to improve the quality of ablation, refine the ablation route, and focus on careful patient selection.

10.
Opt Express ; 32(6): 8623-8637, 2024 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-38571117

RESUMO

In fiber-terahertz integrated communication systems, nonlinear distortion and inter-symbol interference (ISI) will degrade transmission performance. Pre-compensation is an efficient method to handle the channel distortion as it can avoid noise boosting during channel compensation and reduce receiver side signal processing algorithmic complexity at user-end (UE) considering the asymmetric access scenario. In this paper, we propose and experimentally demonstrate a neural-network (NN)-based carrier-less amplitude phase (CAP) modulated signal generation and end-to-end optimization method for a fiber-terahertz integrated communication system. The CAP signal is generated directly from quadrature amplitude modulation symbols and pre-compensated through a transmitter NN, which allows the receiver to demodulate the signal with simple linear digital signal process (DSP). In generating the CAP signal, the NN based transmitter learns a group of filters, which can generate, up-convert, and pre-compensate the signals. Based on the proposed method, a fiber-terahertz integration access system at 220 GHz is demonstrated and a sensitivity gain of 1.2 dB is achieved at a transmission speed of 50 Gbps and the forward error correction (FEC) bit error rate (BER) threshold of 1 × 10-2 compared with the baseline after 10-km fiber transmission and 1-m wireless delivering.

11.
Opt Express ; 32(4): 6309-6328, 2024 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-38439337

RESUMO

Visible light communication (VLC) benefits from the underwater blue-green window and holds immense potential for underwater wireless communication. In order to address the limitations of various equipment and harsh channel conditions in the underwater visible light communication (UVLC) system, the researchers proposed to use the method of autoencoder (AE) to tap the potential of the system. However, traditional AE schemes involve replacing the transmitting and receiving components of a communication system with a large multilayer perceptron (MLP) network, and they have significant drawbacks due to their reliance on a single network structure. In this paper, a novel 2D adaptive optimization autoencoder (2D-AOAE) framework is proposed to realize adaptive modulation and demodulation of two-dimensional signals. By implementing this scheme, we experimentally achieved a transmission rate of 2.85 Gbps over a 1.2-meter underwater VLC link. Compared to the traditional 32QAM UVLC system, the 2D-AOAE scheme demonstrated a 15.4% data rate increase. Moreover, the 2D-AOAE scheme exhibited a remarkable 73% improvement when compared to the UVLC system utilizing the traditional AE scheme. This significant enhancement highlights the superior performance and capabilities of the 2D-AOAE scheme in terms of transmission rate.

12.
Dig Dis Sci ; 69(5): 1889-1896, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38517560

RESUMO

AIM: Endoscopic necrosectomy has become the first-line treatment option for infectious necrotizing pancreatitis (INP), especially walled-off necrosis. However, the problems, including operation-related adverse events (AEs) and the need for multiple endoscopic procedures, have not been effectively addressed. We sought to evaluate the clinical safety and efficacy of anhydrous ethanol-assisted endoscopic ultrasound (EUS)-guided transluminal necrosectomy in INP. METHODS: A single-center observational cohort study of INP patients was conducted in a tertiary endoscopic center. Anhydrous ethanol-assisted EUS-guided transluminal necrosectomy (modified group) and conventional endoscopic necrosectomy (conventional group) were retrospectively compared in INP patients. The technical and clinical success rates, operation time, perioperative AEs, postoperative hospital stay, and recurrent INP rates were analyzed, respectively. RESULTS: A total of 55 patients were enrolled. No statistically significant differences were observed between the two groups regarding baseline characteristics. Compared to patients in the conventional group, patients in the modified group demonstrated significantly reduced times of endoscopic transluminal necrosectomies (1.96 ± 0.89 vs. 2.73 ± 0.98; P = 0.004) and comparable perioperative AEs (P = 0.35). Meanwhile, no statistically significant differences were observed in the technical and clinical success rates (P = 0.92), operation time (P = 0.59), postoperative hospital stay (P = 0.36), and recurrent INP rates (P = 1.00) between the two groups. CONCLUSION: Anhydrous ethanol-assisted EUS-guided transluminal necrosectomy seemed safe and effective in treating INP. Compared with conventional endoscopic transluminal necrosectomy, its advantage was mainly in reducing the number of endoscopic necrosectomies without increasing perioperative AEs.


Assuntos
Endossonografia , Etanol , Pancreatite Necrosante Aguda , Humanos , Pancreatite Necrosante Aguda/cirurgia , Pancreatite Necrosante Aguda/diagnóstico por imagem , Masculino , Feminino , Pessoa de Meia-Idade , Etanol/administração & dosagem , Endossonografia/métodos , Estudos Retrospectivos , Adulto , Resultado do Tratamento , Idoso , Tempo de Internação/estatística & dados numéricos , Ultrassonografia de Intervenção/métodos , Duração da Cirurgia
13.
World J Gastrointest Endosc ; 16(1): 29-36, 2024 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-38313456

RESUMO

BACKGROUND: Gastric variceal hemorrhage is one of the primary manifestations of left-sided portal hypertension (LSPH). The hemorrhage is fatal and requires safe and effective interventions. AIM: To evaluate the clinical safety and efficacy of modified endoscopic ultrasound (EUS)-guided selective N-butyl-2-cyanoacrylate (NBC) injections for gastric variceal hemorrhage in LSPH. METHODS: A retrospective observational study of patients with LSPH-induced gastric variceal hemorrhage was conducted. Preoperative EUS evaluations were performed. Enrolled patients were divided into modified and conventional groups according to the NBC injection technique. The final selection of NBC injection technique depended on the patients' preferences and clinical status. The technical and clinical success rates, operation time, NBC doses, perioperative complications, postoperative hospital stay, and recurrent bleeding rates were analyzed, respectively. RESULTS: A total of 27 patients were enrolled. No statistically significant differences were observed between the two groups regarding baseline characteristics. In comparison to patients in the conventional group, patients in the modified group demonstrated significantly reduced NBC doses (2.0 ± 0.6 mL vs 3.1 ± 1.0 mL; P = 0.004) and increased endoscopic operation time (71.9 ± 11.9 min vs 22.5 ± 6.7 min; P < 0.001). Meanwhile, the two groups had no significant difference in the technical and clinical success rates, perioperative complications, postoperative hospital stay, and recurrent bleeding rates. CONCLUSION: Modified EUS-guided selective NBC injections demonstrated safety and efficacy for LSPH-induced gastric variceal hemorrhage, with advantages of reduced injection dose and no radiation risk. Drawbacks were time consumption and technical challenge.

14.
Pancreatology ; 24(3): 357-362, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38369393

RESUMO

BACKGROUND AND AIM: Endoscopic ultrasound (EUS)-guided endoscopic necrosectomy is an effective and minimally invasive treatment for walled-off pancreatic necrosis (WON). This study investigated the factors affecting the time interval of EUS-guided WON necrosectomy. METHODS: Patients who received EUS-guided necrosectomy in the Endoscopy Center of the First Affiliated Hospital of Chongqing Medical University in the past 5 years were retrospectively analyzed. Data including general information, etiology, blood biochemical indexes, physical signs, CT severity grade, location, size, solid necrotic ratio, type and number of stents, and immediate necrosectomy were collected to explore the relationships between these factors and the interval of endoscopic necrosectomy. RESULTS: A total of 51 WON patients were included. No significant correlation has been noted between the endoscopic debridement interval and the following indexes, including the patients' general information, the etiology of pancreatitis, blood biochemical indexes (leukocyte count, neutrophil percentage, C-reactive protein), preoperative fever, and WON's location and size, type and number of stents, and whether immediate necrosectomy. However, there were significant differences between the debridement interval and the modified CT Severity Index (MCTSI) (p < 0.001), the solid necrotic ratio of WON (p < 0.001) before the intervention, postoperative fever (p = 0.038), C-reactive protein increasing (p = 0.012) and fever before reintervention (p = 0.024). CONCLUSIONS: The EUS-measured solid necrotic ratio, the MCTSI, postoperative fever, C-reactive protein increase, and fever before reintervention in patients affect the time interval of EUS-guided endoscopic necrosectomy in WON patients. These five indicators may be promisingly effective in predicting and managing endoscopic necrosectomy intervals.


Assuntos
Pancreatite Necrosante Aguda , Humanos , Pancreatite Necrosante Aguda/terapia , Estudos Retrospectivos , Proteína C-Reativa , Endossonografia , Stents , Endoscopia Gastrointestinal , Resultado do Tratamento , Ultrassonografia de Intervenção , Drenagem , Necrose
15.
Nat Commun ; 15(1): 1498, 2024 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-38374085

RESUMO

Multimode fiber (MMF) which supports parallel transmission of spatially distributed information is a promising platform for remote imaging and capacity-enhanced optical communication. However, the variability of the scattering MMF channel poses a challenge for achieving long-term accurate transmission over long distances, of which static optical propagation modeling with calibrated transmission matrix or data-driven learning will inevitably degenerate. In this paper, we present a self-supervised dynamic learning approach that achieves long-term, high-fidelity transmission of arbitrary optical fields through unstabilized MMFs. Multiple networks carrying both long- and short-term memory of the propagation model variations are adaptively updated and ensembled to achieve robust image recovery. We demonstrate >99.9% accuracy in the transmission of 1024 spatial degree-of-freedom over 1 km length MMFs lasting over 1000 seconds. The long-term high-fidelity capability enables compressive encoded transfer of high-resolution video with orders of throughput enhancement, offering insights for artificial intelligence promoted diffusive spatial transmission in practical applications.

16.
PLoS One ; 19(2): e0296859, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38416719

RESUMO

To assess the possible impact of climatic variation on microbial community composition in organic winemaking, we employed a metabarcoding approach to scrutinize the microbiome in a commercial, organic, Pinot noir wine production system that utilizes autochthonous fermentation. We assessed microbial composition across two vintages (2018 and 2021) using biological replicates co-located at the same winery. Microbial dynamics were monitored over four important fermentation time points and correlated with contemporaneous climate data. Bacterial (RANOSIM = 0.4743, p = 0.0001) and fungal (RANOSIM = 0.4738, p = 0.0001) compositions were different in both vintages. For bacteria, Lactococcus dominated the diversity associated with the 2018 vintage, while Tatumella dominated the 2021 vintage. For fungal populations, while Saccharomyces were abundant in both vintages, key differences included Starmerella, copious in the 2018 vintage; and Metschnikowia, substantive in the 2021 vintage. Ordination plots correlated the climatic variables with microbial population differences, indicating temperature as a particularly important influence; humidity values also differed significantly between these vintages. Our data illustrates how climatic conditions may influence microbial diversity during winemaking, and further highlights the effect climate change could have on wine production.


Assuntos
Microbiota , Saccharomyces , Vitis , Vinho , Vinho/análise , Bactérias/genética , Fermentação , Vitis/microbiologia
17.
Opt Express ; 32(3): 4201-4214, 2024 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-38297626

RESUMO

Multimode fibers (MMF) show tremendous potential in transmitting high-capacity spatial information. However, the quality of multimode transmission is quite sensitive to inherent scattering characteristics of MMF and almost inevitable external perturbations. Previous research has shown that deep learning may break through this limitation, while deep neural networks are intricately designed with huge computational complexity. In this study, we propose a novel feature decoupled knowledge distillation (KD) framework for lightweight image transmission through MMF. In this framework, the frequency-principle-inspired feature decoupled module significantly improves image transmission quality and the lightweight student model can reach the performance of the sophisticated teacher model through KD. This work represents the first effort, to the best of our knowledge, that successfully applies a KD-based framework for image transmission through scattering media. Experimental results demonstrate that even with up to 93.4% reduction in model computational complexity, we can still achieve averaged Structure Similarity Index Measure (SSIM) of 0.76, 0.85, and 0.90 in Fashion-MNIST, EMNIST, and MNIST images respectively, which are very close to the performance of cumbersome teacher models. This work dramatically reduces the complexity of high-fidelity image transmission through MMF and holds broad prospects for applications in resource-constrained environments and hardware implementations.

18.
Opt Express ; 32(2): 2561-2573, 2024 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-38297782

RESUMO

With the advent of the sixth-generation mobile communication standard (6 G), the visible light communication (VLC) technology based on wavelength division multiplexing (WDM) technology can effectively solve the problem of shortage of spectrum resources and insufficient channel capacity. This paper introduces one of our technical achievements, namely the construction of a near-real-time visible light laser communication (VLLC) system based on WDM, which includes a self-designed 10-λ fully-packaged visible light laser emission module, 1 m multimode fiber - 0.175 m free space - 1 m multimode fiber optical transmission link, and receiver array. In the transmitter system, we adopt adaptive discrete multitone (DMT) modulation technique combined with Quadrature Amplitude Modulation (QAM) modulation scheme to obtain maximum spectral efficiency (SE). In the receiving system, we utilize the sparse-structured reservoir computing post-equalization algorithm to achieve superior equalization performance on the basis of the traditional post-equalization algorithm. The experimental results indicate that this quasi-real-time communication system has achieved a signal transmission rate of 113.175Gbps. To the best of our knowledge, this work has set a record in the field of high-speed visible light laser communication. Therefore, the laser communication system constructed by this work, with its flexibility in deployment and high-speed performance, demonstrates the significant potential application of visible light laser communication in data center interconnection and high-speed indoor access networks.

19.
World J Gastrointest Oncol ; 16(1): 8-12, 2024 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-38292847

RESUMO

This editorial comments on the article "Efficacy of multi-slice spiral computed tomography in evaluating gastric cancer recurrence after endoscopic submucosal dissection". We focus on the importance of paying more attention to post-endoscopic submucosal dissection (ESD) gastric cancer recurrence in patients with early gastric cancer (EGC) and how to manage it effectively. ESD has been a well-known treatment and the mainstay for EGC, with the advantages of less invasion and fewer complications when compared with traditional surgical procedures. Despite a lower local recurrence rate after ESD, the problem of postoperative recurrence in patients with EGC has become increasingly non-ignorable with the global popularization of ESD technology and the increasing number of post-ESD patients.

20.
Int J Biol Macromol ; 257(Pt 2): 128670, 2024 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-38070794

RESUMO

In this study, a novel nano-drug delivery system (CS-Au NPs) based on gold nanoparticles (Au NPs) and chitosan (CS) that modified Myricaria germanica polysaccharide (MGP) was developed to enhance immune responses. At a MGP to CS Au ratio of 5:1, CS-Au-MGP NPs had a loading capacity of 78.27 %. The structure of CS-Au-MGP NPs were characterized by Transmission electron microscope, TEM-energy dispersive spectroscopy mapping, Fourier transform infrared spectroscopy, X-ray photoelectron spectrometer, particle size and zeta-potential distribution analysis. Under weakly acidic conditions, in vitro CS-Au-MGP NPs release was most effective. In vivo showed that co-immunization with CS-Au-MGP NPs and PCV2 significantly increased the organ index of the thymus, spleen, and liver in mice. Additionally, CS-Au-MGP NPs significantly increased the levels of IgG, IgG1, and IgG2a antibodies, as well as IFN-γ and IL-6 levels. Furthermore, the CS-Au-MGP NPs promoted proliferation of spleen T and B lymphocytes, increased the number of CD3+, CD4+, and CD8+ cells, and increased the CD4+/CD8+ T cell ratio. Meanwhile, CS-Au-MGP NPs remarkably TLR2/IRAK4 pathway activation and mRNA levels of cytokines (IFN-γ and IL-6). These results indicated that CS-Au-MGP NPs could enhance the immune activity, and it could be potentially used as an MGP delivery system for the induction of strong immune responses.


Assuntos
Quitosana , Nanopartículas Metálicas , Nanopartículas , Camundongos , Animais , Quitosana/química , Ouro/química , Interleucina-6 , Nanopartículas/química , Polissacarídeos/farmacologia , Imunidade
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