Control of defensive behavior by the nucleus of Darkschewitsch GABAergic neurons.

The nucleus of Darkschewitsch (ND), mainly composed of GABAergic neurons, is widely recognized as a component of the eye-movement controlling system. However, the functional contribution of ND GABAergic neurons (NDGABA) in animal behavior is largely unknown. Here, we show that NDGABA neurons were selectively activated by different types of fear stimuli, such as predator odor and foot shock. Optogenetic and chemogenetic manipulations revealed that NDGABA neurons mediate freezing behavior. Moreover, using circuit-based optogenetic and neuroanatomical tracing methods, we identified an excitatory pathway from the lateral periaqueductal gray (lPAG) to the ND that induces freezing by exciting ND inhibitory outputs to the motor-related gigantocellular reticular nucleus, ventral part (GiV). Together, these findings indicate the NDGABA population as a novel hub for controlling defensive response by relaying fearful information from the lPAG to GiV, a mechanism critical for understanding how the freezing behavior is encoded in the mammalian brain.

Improving suppressive activity of compost on phytopathogenic microbes by inoculation of antagonistic microorganisms for secondary fermentation.

Antimicrobial activity contributes to plant disease control property of composts but its source is still not clear. From composting cow manure during secondary fermentation, 50 microbial strains with antifungal activity were isolated and identified. Two bacterial strains Bacillus mojavensis B282 and Pseudomonas aeruginosa F288, antagonistic against both phytopathogenic fungi and bacteria, were respectively used as the inoculum of compost for secondary fermentation. Inoculation of B282 or F288 significantly shifted microbial community structure of compost and genera functionally linked to antagonistic activity and plant growth promotion were enriched. Notably, culturable cells of B282 increased by about 40 times during secondary fermentation. The inoculation of each strain significantly increased antifungal activity of compost extracts and enhanced disease suppressive effects of compost on wheat root rot. This study demonstrates that inoculation of compost-indigenous microorganisms could improve antimicrobial activity of compost and provides a low-cost strategy for producing bio-organic fertilizers with biocontrol function.

Characterization of Alcaligenes aquatilis as a novel member of heterotrophic nitrifier-aerobic denitrifier and its performance in treating piggery wastewater.

A novel strain AS1 with heterotrophic nitrifying-aerobic denitrifying capacity in the species of Alcaligenes aquatilis was isolated from the aerobic activated sludge. It showed a great capability of ammonia removal, and the aerobic metabolic pathways to yield gaseous-nitrogen by hydroxylamine oxidation and nitrite denitrification were proposed. AS1 could efficiently remove ammonia under a wide range of environmental conditions, including the ratio of chemical oxygen demand to total nitrogen: 15-30, pH: 6-10, NaCl: 0-60 g/L, shaking speed of 0-180 rpm, and succinate, acetate, or citrate as carbon source. In the treatment of actual piggery wastewater, 95.3%, 95.1% and 84.9% of NH4+-N was removed by AS1 when the initial ammonia concentration was 500, 1300, and 2000 mg/L, respectively, with the maximum NH4+-N removal rate of 30.5 mg/L/h and 569.7 mg/L/d. Furthermore, plate colony-counting showed that AS1 achieved an efficient proliferation. These results imply the application potential of AS1 in treating high-ammonia wastewater.