RESUMO
The present study aimed to investigate the antitumor effects of an intratumoral injection of dendritic cells (DCs) overexpressing interleukin-12 (IL12) on melanoma. DCs, isolated from mouse spleen, were genemodified using an IL12 overexpression vector. Melanoma B6 cells were injected into C57BL/6 mice to generate tumors. Thereafter, DCs overexpressing IL12 were injected into the tumors, and tumor volume was subsequently measured. Pathological changes in tumor tissue were detected by hematoxylin and eosin staining. The expression of interleukin-4 (IL4) and IL12 in tumors was measured by enzymelinked immunosorbent assay, realtime PCR and western blotting. DCs were successfully isolated and a lentivirus vector expressing IL12 was constructed. After intratumoral injection of phosphatebuffered saline (control group), tumor cells exhibited malignant growth; whereas tumors injected with DCs (DC group) or DCs + empty vector (DC + vector group) exhibited a small amount of inflammatory cell infiltration and limited areas of tissue necrosis. In contrast, tumors injected with DCs overexpressing IL12 (DC + IL12 group) displayed severe tissue necrosis, loss of cell structure, and inflammatory cell infiltration. Compared with the control group, the tumor volumes were significantly lower in the DC, the DC + vector and the DC + IL12 groups, while the expression of IL12 and IL4 in the tumors was significantly higher. Importantly, the most marked changes in tumor volume and IL12 and IL4 expression were in the DC + IL12 group, which were significantly greater than those in tumors treated with unmodified DCs. Hence, intratumoral injection of DCs overexpressing IL12 exerted strong antitumor effects in melanoma, and biotherapy with DCs overexpressing IL12 is a potential treatment strategy for melanoma.