RESUMO
AIM: Determination of the degree of phylogenetic relationship of Yersinia pestis strains iso- lated from the territories of natural foci of plague from the Caucasus using VNTR-typing by 25 loci (MLVA25). MATERIALS AND METHODS: 26 strains of Y pestis from Russian natural foci of the Caucasus were used in the study. 25 loci of tandem repeats in Y pestis genome by Le Fleche scheme were used for execution of multi-locus VNTR-analysis. Deciphering of nucleotide sequences was carried out in automatic sequencer ABI 3130 Genetic Analyser. Analysis of confinement of clusters to certain territories, objects and time of isolation of strains was carried out. using Arc GIS 10.1 program. RESULTS: Groups of MLVA25-types of various levels of discrimination were formed: clusters, groups and subgroups. Clusters were formed by strains ofvarious taxonomic membership: main and subspecies of Y pestis. Subgroups reflect membership of strains in certain foci, and MLVA25-types - the degree of genetic relationship. CONCLUSION: Genetic <
Assuntos
Loci Gênicos , Filogenia , Peste/genética , Yersinia pestis , Animais , Humanos , Peste/epidemiologia , Federação Russa/epidemiologia , Yersinia pestis/classificação , Yersinia pestis/genética , Yersinia pestis/isolamento & purificaçãoRESUMO
Two plasmid variants of the main subspecies of the plague microbe circulate in a Central Caucasian high-altitude natural focus of plague. The strains of one plasmid variant fully correspond to the main subspecies of the plague pathogen in their characteristics. Those of the other are auxotrophic for proline, weakly virulent to one or both species of laboratory animals. The mountain subspecies of Citellophilus tesquorum fleas excretes the greatest quantity of plague microbe strains so the investigation of whether unblocked fleas can transmit the plague microbe is of interest.
Assuntos
Insetos Vetores/microbiologia , Peste/microbiologia , Plasmídeos/genética , Sifonápteros/microbiologia , Yersinia pestis/crescimento & desenvolvimento , Altitude , Animais , Camundongos , Viabilidade Microbiana , Peste/transmissão , Prolina/metabolismo , Estações do Ano , Yersinia pestis/genética , Yersinia pestis/patogenicidadeRESUMO
The scheme of the complex microbiological diagnostics of cholera, dysentery, salmonellosis and escherichiosis has been developed. The scheme includes the introduction of material taken from a patient with acute enteric infection into 1% peptone water (pH 8.0), followed by inoculation into Endo medium (pH 7.4-8.0) and the selection of colonies on MC-agar with the subsequent final identification of bacteria with the use of biochemical, serological and genetic methods of investigation. This scheme is proposed for use in bacteriological sections of specialized antiepidemic brigades working on the liquidation of the consequences of emergency situations when the epidemiological safety of the population is threatened.
Assuntos
Bactérias/isolamento & purificação , Infecções Bacterianas/microbiologia , Desastres , Gastroenteropatias/microbiologia , Ágar , Infecções Bacterianas/diagnóstico , Técnicas Bacteriológicas , Cólera/microbiologia , Meios de Cultura , Surtos de Doenças/prevenção & controle , Serviços Médicos de Emergência , Infecções por Enterobacteriaceae/microbiologia , Escherichia coli/isolamento & purificação , Gastroenteropatias/diagnóstico , Guias como Assunto , Humanos , Concentração de Íons de Hidrogênio , Peptonas , Salmonella/isolamento & purificação , Shigella/isolamento & purificação , Vibrio cholerae/isolamento & purificaçãoRESUMO
The results of complete identification revealed the phenotypical similarity of V. cholerae strains isolated from surface water reservoirs im Grozny in 1995 and 2000. The strains under study proved to be atoxigenic, hemolysis-positive and phage resistant. The modified method of subculturing from peptone water 1 to peptone water 2 was more labor-consuming, but it was used in the bacteriological laboratory of the specialized antiepidemic brigade with the aim of more profound study of open reservoirs for the presence of V. cholerae with the positive result.
Assuntos
Vibrio cholerae O1/classificação , Microbiologia da Água , Proteínas de Bactérias , Técnicas Bacteriológicas/métodos , Meios de Cultura , Proteínas Hemolisinas , Peptonas , Fenótipo , Federação Russa , Especificidade da Espécie , Vibrio cholerae O1/imunologia , Vibrio cholerae O1/isolamento & purificação , GuerraRESUMO
The use of new diagnostic preparations (magnetic immunosorbents, polyvalent fluorescent immunoglobulins and immunoperoxidase conjugates), as well as the combination of magnetic immunosorbents with the polymerase chain reaction, for the rapid diagnostics of dangerous infectious diseases has been shown to have good prospects. Information on their successful use for laboratory diagnostics in emergency situations is presented.