RESUMO
A comparative investigation on the photophysical properties and solvation-related ICT dynamics of three push-pull compounds containing different donors including carbazole, triphenylamine and phenothiazine, was performed. The steady-state spectra and theoretical calculations show the charge transfers from the central donors to the acceptors at each side. The characterization of the extent of charge transfer was determined by various means, including estimation of the dipole moment, the electron density distribution of HOMO and LUMO, CDD and change in Gibb's free energy, which show the charge transfer strength to be in the order PDHP > BDHT > PDHC. This suggests that the electron-donating ability of the donor groups plays a crucial role in the charge transfer in these compounds. The TA data show the excited-state relaxation dynamics follow a sequential model: FCâICTâICT'âS0 , and are affected by the solvent polarity. The results presented here demonstrate that the compound with a higher degree of ICT characteristic interacts more strongly with stronger polar solvent molecules, which can accelerate the solvation and spectral evolution to lower energy levels. The A-π-D-π-A architectures with prominent ICT characteristics based on carbazole, triphenylamine and phenothiazine might be potential scaffolds for light-harvesting and photovoltaic devices. These results are of value for understanding structure-property relationships and the rational design of functional materials for photoelectric applications.
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Excited state solvation plays a very important role in modulating the emission behavior of fluorophores upon excitation. Here, the solvation effects on the local micro-environment around a fluorophore are proposed by investigating the fantastic emission behavior of a novel amyloid fibril marker, NIAD-4, in different alcoholic and aprotic solvents. In alcoholic solvents, high solvent viscosity causes an obvious enhancement of fluorescence because of the restriction of torsion of NIAD-4, where the formation of a non-fluorescent twist intramolecular charge transfer (TICT) state is suppressed. In aprotic solvents, high solvent polarity leads to a remarkable redshift of the emission spectra suggesting strong solvation. Surprisingly, an abnormal fluorescence enhancement of NIAD-4 is observed with increasing solvent polarity of the aprotic solvents, whereas solvent viscosity plays little role in influencing the fluorescence intensity. We conclude that such an abnormal phenomenon is originated from a solvation induced micro-viscosity enhancement around the fluorophore upon excitation which restricts the torsion of NIAD-4. Femtosecond transient absorption results further prove such a micro-viscosity increasing mechanism. We believe that this solvation induced micro-viscosity enhancement effect on fluorescence could widely exist for most donor-π-acceptor (D-π-A) compounds in polar solvents, which should be carefully taken into consideration when probing the micro-viscosity in polar environments, especially in complex bioenvironments.
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We report the excited-state intramolecular charge transfer (ICT) characteristics of four tetrahydro[5] helicene-based imide (THHBI) derivatives with various electron-donating substitutes in different polarity of solvents using steady-state, time-resolved transient absorption (TA) spectroscopy. It is found that, the small bathochromic-shift of the absorption spectra but large red shift of the emission spectra for all dyes with increasing solvent polarity indicates the larger dipole moment of the excited state compared to ground state. The results of theoretical calculations exhibit the charge transfer from the terminal donors to helical backbone, which accounts for the degrees of red shift of the emission spectra from different extent of ICT nature. Time-resolved TA spectra recorded as a function of electron-donating substitutes and solvent polarity show the dye with stronger donors (THHBI-PhNPh2) in more polar solvent behaves faster excited-state ICT relaxation, leading to the formation of solvent-stabilized ICT state (ICT' state) from the excited ICT state; The dyes (THHBI-Ph, THHBI-PhCF3 and THHBI-PhOMe) with relative weaker donors show weaker dependence on solvent polarity, and instead of that intersystem crossing (ISC) becomes possible from ICT state to triplet state.
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The photophysical properties of three octupolar chromophores containing planar triazatruxene (TAT) as the central electron donor with different electron-withdrawing groups in the tribranched arrangement have been systematically investigated by means of steady state and transient spectroscopy. The multidimensional intramolecular charge transfer (ICT) properties of these tribranched chromophores related to the observed two-photon absorption (TPA) properties are explored by estimating the TPA essential factors (Mge and Δµge). Besides the large Stokes shift between steady state absorption and fluorescence spectra in different polar solvents, photoinduced ICT was further demonstrated by quantum-chemical calculations and transient absorption measurements. Both quantum calculations and spectral experiments show that a multidimensional ICT occurs from the electron-rich core to the electron-deficient periphery of these TAT derivatives. The results of solvation effects and the dynamics of the excited states show that the excited states of these three chromophores tend to exhibit an excitation localization on one of the dipolar branches, which is beneficial to achieve large Mge and Δµge, thus leading to enhanced TPA properties.
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The excited-state relaxation dynamics and chromophore interactions in two phthalocyanine compounds (bis- and trisphthalocyanines) are studied by using steady-state and femtosecond transient absorption spectral measurements, where the excited-state energy-transfer mechanism is explored. By exciting phthalocyanine compounds to their second electronically excited states and probing the subsequent relaxation dynamics, a multitude of deactivation pathways are identified. The transient absorption spectra show the relaxation pathway from the exciton state to excimer state and then back to the ground state in bisphthalocyanine (bis-Pc). In trisphthalocyanine (tris-Pc), the monomeric and dimeric subunits are excited and the excitation energy transfers from the monomeric vibrationally hot S1 state to the exciton state of a pre-associated dimer, with subsequent relaxation to the ground state through the excimer state. The theoretical calculations and steady-state spectra also show a face-to-face conformation in bis-Pc, whereas in tris-Pc, two of the three phthalocyanine branches form a pre-associated face-to-face dimeric conformation with the third one acting as a monomeric unit; this is consistent with the results of the transient absorption experiments from the perspective of molecular structure. The detailed structure-property relationships in phthalocyanine compounds is useful for exploring the function of molecular aggregates in energy migration of natural photosynthesis systems.
Assuntos
Indóis/química , Isoindóis , Estrutura Molecular , Espectrofotometria UltravioletaRESUMO
Femtosecond coherent anti-Stokes Raman scattering (fsCARS) spectroscopy, together with perturbation theory based numerical calculation, is employed to study OH stretching (υOH) of pure water and aqueous lithium chloride solutions. Vibrational OH stretching (υOH) modes of aqueous solutions are Raman-excited by a pair of ultrashort, femtosecond laser pulses, and then probed through inelastic scattering of a third, time-delayed laser field. In order to overcome limited spectral resolution of fsCARS, numerical evaluation of the CARS signal through vibrational wave packet propagation was employed in order to confirm the position of distinctive OH stretching mode that is complicated by intramolecular and intermolecular vibrational coupling. Moreover, in order to come to a microscopic description of the observed CARS spectra for aqueous solutions, we have performed molecular dynamics simulations of aqueous lithium chloride solutions with varying concentrations at ambient conditions. To this end we have analyzed the equilibrium distributions of hydrogen bonds in the first solvation shells of the ions as well as in bulk water and also computed the average number of hydrogen bonds per water molecule. According to our experimental and theoretical results on time evolution of Raman OH stretching band of water, it can be inferred that the dissolved ions mainly influence hydrogen bond strength and structure of water molecules in the first hydration shell, the addition of lithium chloride primarily breaks the tetrahedral hydrogen bonding, promotes formation of the donor hydrogen bonding in water, and slightly increases the amount of free OH bonds.
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The photophysical properties of intramolecular charge transfer (ICT) in a novel tribranched donor-π-acceptor chromophore, triphenoxazine-2,4,6-triphenyl-1,3,5-triazine (tri-PXZ-TRZ), with thermally activated delayed fluorescence character was investigated in different aprotic solvents by steady-state spectroscopy and femtosecond and nanosecond transient absorption spectroscopy measurements. Increasing the solvent polarity led to a significant increase in the Stokes shift. The large Stokes shift in highly polar solvents was attributed to ICT properties upon excitation; this resulted in a strong interaction between the tri-PXZ-TRZ molecule and the surrounding solvent, which led to a strong solvation process. Quantum-chemical calculations and changes in the dipole moment showed that this compound has a large degree of ICT. Furthermore, an apolar environment helped to preserve the symmetry of tri-PXZ-TRZ and to enhance its emission efficiency. The femtosecond and nanosecond transient absorption spectroscopy results indicated that the excited-state dynamics of this push-pull molecule were strongly influenced by solvent polarity through the formation of a solvent-stabilized ICT state. The excited-state relaxation mechanism of tri-PXZ-TRZ was proposed by performing target model analysis on the femtosecond transient absorption spectra. In addition, the delayed fluorescence of tri-PXZ-TRZ was significantly modulated by a potential competition between solvation and intersystem crossing processes.
Assuntos
Triazinas/química , Teoria Quântica , Solventes/química , Espectrometria de FluorescênciaRESUMO
Modeling the local absorption and retention patterns of membrane-permeant small molecules in a cellular context could facilitate development of site-directed chemical agents for bioimaging or therapeutic applications. Here, we present an integrative approach to this problem, combining in silico computational models, in vitro cell based assays and in vivo biodistribution studies. To target small molecule probes to the epithelial cells of the upper airways, a multiscale computational model of the lung was first used as a screening tool, in silico. Following virtual screening, cell monolayers differentiated on microfabricated pore arrays and multilayer cultures of primary human bronchial epithelial cells differentiated in an air-liquid interface were used to test the local absorption and intracellular retention patterns of selected probes, in vitro. Lastly, experiments involving visualization of bioimaging probe distribution in the lungs after local and systemic administration were used to test the relevance of computational models and cell-based assays, in vivo. The results of in vivo experiments were consistent with the results of in silico simulations, indicating that mitochondrial accumulation of membrane permeant, hydrophilic cations can be used to maximize local exposure and retention, specifically in the upper airways after intratracheal administration.
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Biologia Computacional/métodos , Pulmão/fisiologia , Sondas Moleculares/farmacologia , Absorção , Animais , Transporte Biológico , Brônquios/citologia , Cátions , Diferenciação Celular , Simulação por Computador , Células Epiteliais/citologia , Corantes Fluorescentes/farmacologia , Humanos , Pulmão/patologia , Camundongos , Microscopia Confocal/métodos , Mitocôndrias/metabolismo , Software , Traqueia/patologiaRESUMO
A biophysical, computational model of cell pharmacokinetics (1CellPK) is being developed to enable prediction of the intracellular accumulation and transcellular transport properties of small molecules using their calculated physicochemical properties as input. To test if 1CellPK can generate accurate, quantitative hypotheses and guide experimental analysis of the transcellular transport kinetics of small molecules, epithelial cells were grown on impermeable polyester membranes with cylindrical pores and chloroquine (CQ) was used as a transport probe. The effect of the number of pores and their diameter on transcellular transport of CQ was measured in apical-to-basolateral or basolateral-to-apical directions, at pH 7.4 and 6.5 in the donor compartment. Experimental and simulation results were consistent with a phospholipid bilayer-limited, passive diffusion transport mechanism. In experiments and 1CellPK simulations, intracellular CQ mass and the net rate of mass transport varied <2-fold although total pore area per cell varied >10-fold, so by normalizing the net rate of mass transport by the pore area available for transport, cell permeability on 3 mum pore diameter membranes was more than an order of magnitude less than on 0.4 mum pore diameter membranes. The results of simulations of transcellular transport were accurate for the first four hours of drug exposure, but those of CQ mass accumulation were accurate only for the first five minutes. Upon prolonged incubation, changes in cellular parameters such as lysosome pH rise, lysosome volume expansion, and nuclear shrinkage were associated with excess CQ accumulation. Based on the simulations, lysosome volume expansion alone can partly account for the measured, total intracellular CQ mass increase, while adding the intracellular binding of the protonated, ionized forms of CQ (as reflected in the measured partition coefficient of CQ in detergent-permeabilized cells at physiological pH) can further improve the intracellular CQ mass accumulation prediction.