Optimal audio beam pattern synthesis for an enhanced parametric array loudspeaker.

A parametric array loudspeaker (PAL) generates highly directional audible sound in air with a small aperture size compared to a conventional loudspeaker. But in indoor applications, the long propagation distance of a PAL causes reflections, which disturbs the reproduction of narrow audio beams. Moreover, sound distortion appears along the off-axis direction due to the frequency dependence of the beam width. This study proposed an optimal audio beam pattern synthesis for a PAL-based convex optimization, which can design the audio beam of a PAL with an optimal solution. The proposed method overcame the mentioned limitations by applying it to a length-limited PAL for audio spot control and a multichannel PAL array for a constant beam width audio beam. In a length-limited PAL, the proposed method restricts the audio spot to a smaller region and weakens the sound leakage along the off-axis direction. Whereas in a multichannel PAL array, the proposed method also achieves a constant beam width near the radiator axis. Simulations and experiments verify the effectiveness of the proposed method, which will enhance the performance of a PAL in scenarios where control of the audio beam is required.

Atmospheric wet and dry depositions of polycyclic aromatic compounds in a megacity of Southwest China.

The dry and wet depositions of polycyclic aromatic hydrocarbons (PAHs) and related derivatives have rarely been characterized separately. Parent, oxygenated and nitrated PAHs (PAHs, OPAHs and NPAHs) have been measured in monthly-averaged wet and dry deposition samples collected from January to December 2019 in urban Chongqing. The annual average concentrations of Æ©17PAHs, Æ©9OPAHs and Æ©9NPAHs in wet deposition samples were 457 ± 375, 1311 ± 1416 and 8.25 ± 10.2 ng/L, respectively, with significant monthly variations introduced by rainfall and air-borne particle deposition. Most PAHs species were associated with the particulate phase in wet deposition, while OPAHs and NPAHs were mainly distributed in the dissolved phase, probably due to the lower octanol-water partitioning coefficient of N/OPAHs than that of PAHs. Annual deposition fluxes of PAHs, OPAHs and NPAHs in dry deposition were 25264, 25310 and 388 ng/m2/yr, respectively, higher than those in wet deposition (9869, 24083 and 207 ng/m2/yr). This indicates that PACs, especially PAHs, were removed from the atmosphere mainly via dry deposition. The contributions of wet deposition to total deposited PACs were larger for months with higher precipitation and for PACs with higher molecular weight. Composition pattern and temporal variation results indicated that wet deposition fluxes were mainly affected by precipitation-related particle deposition and chemical properties (e.g., water solubility), while dry deposition fluxes were affected more by factors such as gas/particle partitioning, particle size distribution and physicochemical properties of PACs. Principle component analysis combined with diagnostic ratios revealed that PACs in atmospheric deposition samples were from vehicle emission (48.6%), coal combustion (13.4%), petrogenic source (5.9%) and secondary formation (32.1%).

Assessing potential risks of aquatic polycyclic aromatic compounds via multiple approaches: A case study in Jialing and Yangtze Rivers in downtown Chongqing, China.

To better evaluate the potential risks of aquatic polycyclic aromatic compounds (PACs), multiple approaches have been implemented in this study to assess the human health and ecological risks of parent, nitrated and oxygenated polycyclic aromatic hydrocarbons (PAHs, NPAHs and OPAHs) in the surface water of Jialing and Yangtze Rivers in downtown Chongqing in southwestern China. The concentrations of ∑PAHs (334 ± 125 ng L-1) were much higher than those of ∑OPAHs (20.2 ± 7.49 ng L-1) in the two rivers, while NPAHs were barely detected. Concentrations of detected PACs were higher in wet season than dry season, probably resulted from the elevated particle input due to heavy rainfall in wet season. Concentrations of PAHs were higher in the particulate phase than dissolved phase, while OPAHs levels showed a reverse pattern. The partition coefficients (Kp) of PACs in the water-SPM (suspended particulate matter) system were mainly affected by SPM concentrations and octanol/water partition coefficients of specific PACs. Human health risks calculated from non-probabilistic risk assessment model and probabilistic risk assessment model based on Monte Carlo simulation showed similar data pattern with slight difference in absolute values. Both models revealed potential or even severe human health risks contributed mainly by dermal exposure to aquatic PACs in this study. Furthermore, these models also manifested that infant stage was highly sensitive for PAC exposure. Sensitivity analysis indicated that health risk results was most sensitive to Benzo[a]pyrene equivalent toxic concentration (BaPeq), followed by showering time and daily water intake volume. Levels of ecological risks and contributions of individual PACs differed from models based on different quality values. The adequacy of toxicity data was crucial for the reliability of ecological risk assessment.

Parabrachial nucleus astrocytes regulate wakefulness and isoflurane anesthesia in mice.

Background: The parabrachial nucleus (PBN) is an important structure regulating the sleep-wake behavior and general anesthesia. Astrocytes in the central nervous system modulate neuronal activity and consequential behavior. However, the specific role of the parabrachial nucleus astrocytes in regulating the sleep-wake behavior and general anesthesia remains unclear. Methods: We used chemogenetic approach to activate or inhibit the activity of PBN astrocytes by injecting AAV-GFAabc1d-hM3Dq-eGFP or AAV-GFAabc1d-hM4Di-eGFP into the PBN. We investigated the effects of intraperitoneal injection of CNO or vehicle on the amount of wakefulness, NREM sleep and REM sleep in sleep-wake behavior, and on the time of loss of righting reflex, time of recovery of righting reflex, sensitivity to isoflurane, electroencephalogram (EEG) power spectrum and burst suppression ratio (BSR) in isoflurane anesthesia. Results: The activation of PBN astrocytes increased wakefulness amount for 4 h, while the inhibition of PBN astrocytes decreased total amount of wakefulness during the 3-hour post-injection period. Chemogenetic activation of PBN astrocytes decreased isoflurane sensitivity and shortened the emergence time from isoflurane-induced general anesthesia. Cortical EEG recordings revealed that PBN astrocyte activation decreased the EEG delta power and BSR during isoflurane anesthesia. Chemogenetic Inhibition of PBN astrocytes increased the EEG delta power and BSR during isoflurane anesthesia. Conclusion: PBN astrocytes are a key neural substrate regulating wakefulness and emergence from isoflurane anesthesia.

Analysis of the community composition and bacterial diversity of the rhizosphere microbiome across different plant taxa.

Rhizobacteria play an important role in bridging the soil and plant microbiomes and improving the health and growth of plants. In this study, the bacterial community structures and compositions of rhizosphere microbiomes associated with six plant species, representing two orders and three families of wild plants grown in the same field, were evaluated. The six plant species examined harbored a core and similar bacterial communities of the rhizosphere microbiome, which was dominated by members of Rhizobiales, Sphingomonadales, Burkholderiales, and Xanthomonadales of Proteobacteria, Subgroup 4 of Acidobacteria, and Sphingobacteriales of Bacteroidetes. Plant species had a significant effect on the microbial composition and Operational Taxonomic Unit (OTU) abundance of the rhizosphere microbiome. Statistical analysis indicated a significant differential OTU richness (Chao1, p < 0.05) and bacterial diversity (Shannon index, p < 0.0001) of the rhizosphere microbiome at the plant species, genus, or families levels. The paralleled samples from the same plant species in the PCoA and hierarchical cluster analysis demonstrated a clear tendency to group together, although the samples were not strictly separated according to their taxonomic divergence at the family or order level. The CAP analysis revealed a great proportion (44.85%) of the variations on bacterial communities could be attributed to the plant species. The results demonstrated that largely conserved and taxonomically narrow bacterial communities of the rhizosphere microbiome existed around the plant root. The bacterial communities and diversity of the rhizosphere microbiome were significantly related to the plant taxa, at least at the species levels.

Genome-Wide Identification of Circular RNAs as a Novel Class of Putative Biomarkers for an Ocular Surface Disease.

BACKGROUND/AIMS: Pterygium is a common ocular surface disease with an unknown etiology and threatens vision as it invades into the cornea. Circular RNAs (circRNAs) are a novel class of RNA transcripts that participate in several physiological and pathological processes. However, the role of circRNAs in pathogenesis of pterygium remains largely unknown. METHODS: Genome-wide circRNA expression profiling was performed to identify pterygium -related circRNAs. GO analysis, pathway analysis, and miRNA response elements analysis was performed to predict the function of differentially expressed circRNAs in pterygium. MTT assays, Ki67 staining, Transwell assay, Hoechst 33342 staining, and Calcein-AM/PI staining were performed to determine the effect of circRNA silencing on pterygium fibroblast and epithelial cell function. RESULTS: Approximately 669 circRNAs were identified to be abnormally expressed in pterygium tissues. GO analysis demonstrated that the host genes of differentially expressed circRNAs were targeted to extracellular matrix organization (ontology: biological process), cytoplasm (ontology: cellular component), and protein binding (ontology: molecular function). Pathway analysis showed that dysregulated circRNAs-mediated regulatory networks were mostly enriched in focal adhesion signaling pathway. Notably, circ_0085020 (circ-LAPTM4B) was shown as a potential biomarker for pterygium. circ_0085020 (circ-LAPTM4B) silencing affected the viability, proliferation, migration, and apoptosis of pterygium fibroblast and epithelial cells in vitro. CONCLUSIONS: This study provides evidence that circRNAs are involved in the pathogenesis of pterygium and might constitute promising targets for the therapeutic intervention of pterygium.

Gefitinib inhibits retina angiogenesis by affecting VEGF signaling pathway.

Gefitinib, a small-molecule multitargeted tyrosine kinase inhibitor, is reported to be as a new oral antiangiogenic molecule. However, it is unknown whether Gefitinib inhibits retina angiogenesis. In this study, we aimed to investigate the effect of Gefitinib on oxygen-induced retinal angiogenesis and the correlation between Gefitinib and VEGF signaling pathway. 12 cases of mice models of oxygen-induced retinopathy were obtained by exposing to 75% oxygen for 7 days. Then, these models were randomly assigned to two groups, and treated with Gefitinib and DMSO, respectively. Retinal vascular morphology was evaluated by Ink staining, HE staining and isolectin staining. Finally, the expression levels of VEGF, Cyclin E, CDK2, CD31 were detected in retinal tissues. Results shown that newborn retinal vessels and oxygen-induced vaso-obliteration were easily to be observed in oxygen-induced groups compared with controls. Meanwhile, the number of nonganglion cells and neovascular nuclei in oxygen-induced groups was significantly increased compared with those in control groups. However, when treated with Gefitinib, newborn retinal vessels in mice models of oxygen-induced retinopathy were significantly reduced. Further investigation confirmed that Gefitinib treatment affected the VEGF/Cyclin E/CDK2/CD31 pathway. In conclusion, these findings indicate that Gefitinib is critical for the treatment of retina angiogenesis, which is associated with VEGF/Cyclin E/CDK2/CD31 pathway.

Long non-coding RNA MEG3 silencing protects against light-induced retinal degeneration.

Excessive light exposure leads to retinal degeneration and accelerates the progression and severity of several ocular diseases, such as age-related macular degeneration (AMD) and retinitis pigmentosa. Long non-coding RNAs (LncRNAs) have emerged as important regulators of photoreceptor development and ocular diseases. In this study, we investigated the role of lncRNA-MEG3 in light-induced retinal degeneration. MEG3 expression was significantly up-regulated after light insult in vivo and in vitro. MEG3 silencing protected against light-induced retinal degeneration in vivo and light-induced photoreceptor cell apoptosis in vitro. Mechanistically, MEG3 regulated retinal photoreceptor cell function by acting as p53 decoy. MEG3 silencing decreased caspase 3/7 activity, up-regulated anti-apoptotic protein (Bcl-2) expression, and down-regulated pro-apoptotic protein (Bax) expression. Taken together, this study provides a promising method of MEG3 silencing for treating light-induced retinal degeneration.

Activation of KGFR-Akt-mTOR-Nrf2 signaling protects human retinal pigment epithelium cells from Ultra-violet.

Ultra-violet (UV) radiation causes oxidative injuries to human retinal pigment epithelium (RPE) cells. We tested the potential effect of keratinocyte growth factor (KGF) against the process. KGF receptor (KGFR) is expressed in ARPE-19 cells and primary human RPE cells. Pre-treatment with KGF inhibited UV-induced reactive oxygen species (ROS) production and RPE cell death. KGF activated nuclear-factor-E2-related factor 2 (Nrf2) signaling in RPE cells, causing Nrf2 Ser-40 phosphorylation, stabilization and nuclear translocation as well as expression of Nrf2-dependent genes (HO1, NOQ1 and GCLC). Nrf2 knockdown (by targeted shRNAs) or S40T mutation almost reversed KGF-induced RPE cell protection against UV. Further studies demonstrated that KGF activated KGFR-Akt-mTORC1 signaling to mediate downstream Nrf2 activation. KGFR shRNA or Akt-mTORC1 inhibition not only blocked KGF-induced Nrf2 Ser-40 phosphorylation and activation, but also nullified KGF-mediated RPE cell protection against UV. We conclude that KGF-KGFR activates Akt-mTORC1 downstream Nrf2 signaling to protect RPE cells from UV radiation.