RESUMO
We studied the formation of spatial and nonspatial memory in mice during learning in three different condensed versions of Morris water maze task. Learning in combined version caused the formation of both spatial and nonspatial memory, whereas learning in condensed versions (spatial and nonspatial) led to memory formation specific for the version.
Assuntos
Memória de Curto Prazo , Memória Espacial , Animais , Masculino , Aprendizagem em Labirinto , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos DBARESUMO
Seeing the big picture of functional responses within large neural networks in a freely functioning brain is crucial for understanding the cellular mechanisms behind the higher nervous activity, including the most complex brain functions, such as cognition and memory. As a breakthrough toward meeting this challenge, implantable fiber-optic interfaces integrating advanced optogenetic technologies and cutting-edge fiber-optic solutions have been demonstrated, enabling a long-term optogenetic manipulation of neural circuits in freely moving mice. Here, we show that a specifically designed implantable fiber-optic interface provides a powerful tool for parallel long-term optical interrogation of distinctly separate, functionally different sites in the brain of freely moving mice. This interface allows the same groups of neurons lying deeply in the brain of a freely behaving mouse to be reproducibly accessed and optically interrogated over many weeks, providing a long-term dynamic detection of genome activity in response to a broad variety of pharmacological and physiological stimuli.
Assuntos
Encéfalo/fisiologia , Tecnologia de Fibra Óptica , Próteses e Implantes , Animais , Encéfalo/efeitos dos fármacos , Tecnologia de Fibra Óptica/instrumentação , Masculino , Camundongos , Camundongos Transgênicos , Neurônios/efeitos dos fármacos , Neurônios/fisiologia , Fibras Ópticas , Fatores de Transcrição/metabolismoRESUMO
We studied the effects of training on DNA synthesis intensity in mouse brain. Brain cells where DNA synthesis-associated processes took place under the influence of training were detected by immunohistochemical labeling of DNA molecules with synthetic thymine analogue 5-bromo-2'-deoxyuridine. The number of 5-bromo-2'-deoxyuridine-positive cell increased in various structures of the brain under the influence of training.
Assuntos
Aprendizagem da Esquiva/fisiologia , Encéfalo/metabolismo , Bromodesoxiuridina/farmacocinética , DNA/biossíntese , Análise de Variância , Animais , Autorradiografia , Bromodesoxiuridina/administração & dosagem , Bromodesoxiuridina/metabolismo , DNA/metabolismo , Imuno-Histoquímica , Injeções Intraperitoneais , Camundongos , Camundongos Endogâmicos C57BL , Estatísticas não ParamétricasRESUMO
We studied the effects of training on DNA synthesis intensity in mouse brain. Brain cells where DNA synthesis-associated processes took place under the influence of training were detected by immunohistochemical labeling of DNA molecules with synthetic thymine analogue 5-bromo-2'-deoxyuridine. The number of 5-bromo-2'-deoxyuridine-positive cell increased in various structures of the brain under the influence of training.