[Risk factors for mesioangular and vertical impactions of mandibular third molars based on logistic regression analysis].

PURPOSE: To explore the risk factors for mesioangular and vertical impactions of the mandibular third molars and to construct a predictive model based on logistic regression analysis. METHODS: Clinical data of 243 mandibular third molars collected from June 2021 to December 2023 at Tianjin Stomatology Hospital were classified into the eruption group and the impaction group, with the latter including mesioangular and vertical unilateral impactions. The clinical data were subjected to univariate analysis to screen for statistically significant factors, followed by multivariate analysis using logistic regression to further delineate risk factors for mandibular third molar impaction, with the construction of a nomogram for prediction.SPSS 27.0 software package was used for statistical analysis. RESULTS: Totally 243 mandibular third molars were included, and 75 (30.86%) were in the eruption group and 168 (69.14%) in the impaction group. No significant difference was found between the groups regarding age, gender, number of tooth roots, Co-Go, Co-Cop, W2, W3, W4 and L (P＞0.05). Significant differences were observed between the eruption and impaction group concerning Nolla, L-6 missing, L-E missing, Co-Pog, Co-Go/Co-Pog, L6-MP, α and W1(P＜0.05). Multivariate regression analysis revealed that Nolla, absence of L-6, absence of L-E, Co-Pog, Co-Go/Co-Pog, L6-MP, α and W1 were independent risk factors for mesioangular and vertical impactions of the mandibular third molars (P＜0.05). The construction of nomogram demonstrated high predictive accuracy. Analysis of the receiver operating characteristic curve(ROC) indicated that the area under the curve(AUC) for the joint prediction of mesial and vertical impaction of the mandibular third molar by independent risk factors was 0.924, with a 95%CI of 0.887 to 0.960. The sensitivity was reported to be 86.9%, and the specificity was 86.7%. CONCLUSIONS: Nolla, absence of L-6, absence of L-E, Co-Pog, Co-Go/Co-Pog, L6-MP, α and W1 are major risk factors affecting the impaction of mandibular third molars. The use of logistic regression analysis and nomograms can effectively predict the risk of impaction, providing a scientific basis for clinical treatment.

Effect of acidic culture conditions on the proliferation, apoptosis, and migration ability of human tongue squamous cell carcinoma cells and its related mechanism.

OBJECTIVES: This study aims to explore the effect of acidic culture conditions on the proliferation, apoptosis, and migration ability of human tongue squamous cell carcinoma SCC15 and CAL27 cells and its potential molecular mechanism. METHODS: After acidic culture for different periods, methyl thiazolyl tetrazolium (MTT) method was adop-ted to detect the cell proliferation of SCC15 and CAL27. Flow cytometry was employed to detect the apoptosis level of SCC15 and CAL27 cells. The migration ability of SCC15 and CAL27 after acidic culture was detected by scratch hea-ling test. Real-time fluorescence quantitative polymerase chain reaction (FQ-PCR) was used to detect the mRNA expression of cyclooxygenase 2 (COX-2) and survivin in SCC15 and CAL27 cells after acidic culture. RESULTS: After culture for 24 h under acidic microenvironment, SCC15 and CAL27 cells grew rapidly and reached the stationary phase after adjustment for 3 days. The apoptosis levels of SCC15 and CAL27 cells decreased after acidic culture, but the most significant reduction occurred after 6 h of acidic culture. The scratch healing rates of SCC15 and CAL27 cells increased after acidic culture. The results of FQ-PCR showed that the mRNA expression levels of COX-2 and survivin in SCC15 and CAL27 cells increased after acidic culture. CONCLUSIONS: Extracellular acidic microenvironment can inhibit the apoptosis of tongue squamous carcinoma cells, promote their migration, and induce more adaptable and malignant tongue squamous carcinoma cells. The mechanism may be related to COX-2 and survivin and their signal pathways.

Effects of Shuanghuangbu on the total protein content and ultrastructure in cultured human periodontal ligament cells.

BACKGROUND: Successful periodontal regeneration depends on the migration, proliferation and differentiation of periodontal ligament cells in periodontal defects. The total protein content and the ultrastructure demonstrate the metabolizability and activity of periodontal ligament cells. This study was conducted to observe the effects of Shuanghuangbu, a mixture of medicinal herbs, on the total protein content and the ultrastructure of human periodontal ligament cells. METHODS: Periodontal ligament cells were grown to confluence and then cultured in Dulbecco's modified eagle medium (DMEM) supplemented with Shuanghuangbu over the concentration range of 0 to 1000 microg/ml. The total protein content in cultured cells was determined by using Coommasie brilliant blue technique. Periodontal ligament cells were incubated in 0 and 100 microg/ml Shuanghuangbu decoction for 5 days, then observed through transmission electron microscope. RESULTS: The total protein content of human periodontal ligament cells increased in each experiment group added 10 - 1000 microg/ml Shuanghuangbu respectively, and the effect of 100 microg/ml was excellent. Under the transmission electron microscope, there were more rough endoplasmic reticulums and mitochodrias in the experiment group than those in the control group. CONCLUSION: Shuanghuangbu stimulates the protein synthesis of human periodontal ligament cells and improves human periodontal ligament cells' metabolizability and activity.