Long-range optofluidic control with plasmon heating.

Using light to manipulate fluids has been a long-sought-after goal for lab-on-a-chip applications to address the size mismatch between bulky external fluid controllers and microfluidic devices. Yet, this goal has remained elusive due to the complexity of thermally driven fluid dynamic phenomena, and the lack of approaches that allow comprehensive multiscale and multiparameter studies. Here, we report an innovative optofluidic platform that fulfills this need by combining digital holographic microscopy with state-of-the-art thermoplasmonics, allowing us to identify the different contributions from thermophoresis, thermo-osmosis, convection, and radiation pressure. In our experiments, we demonstrate that a local thermal perturbation at the microscale can lead to mm-scale changes in both the particle and fluid dynamics, thus achieving long-range transport. Furthermore, thanks to a comprehensive parameter study involving sample geometry, temperature increase, light fluence, and size of the heat source, we showcase an integrated and reconfigurable all-optical control strategy for microfluidic devices, thereby opening new frontiers in fluid actuation technology.

[Delphi study to identify the management skills of nursing executives]. / Estudio Delphi para identificar las competencias en gestión del directivo de enfermería.

OBJECTIVE: To determine and update the skills map for the position of Nurse Administrator in hospitals and Primary Care. METHOD: An observational, descriptive, cross-sectional study based on a Delphi technique was conducted in hospital and Primary Care settings. Two nominal groups with 15 nurses each were used to define the contents of the questionnaire 0 in the Delphi technique. All nurses registered in the professional associations of Alicante, Castellón and Valencia were invited to participate. The results of the Delphi study was submitted to factor analysis to identify the set of skills and, subsequently, compare them with the offer of post-graduate course in colleges and universities during the 2014-15 academic year. RESULTS: Forty-five competences were extracted during the Nominal groups. In total, 705 nurses replied to the first wave in the Delphi Technique, and 394 in the second (response rate of 56%). Factorial analysis grouped the skills chosen into 10 factors: managing people, conflict management, independent learning, ethics, emotional balance, commitment, self-discipline, continuous improvement, critical-thinking, and innovation. Four skills groups identified in this study (emotional balancing, commitment, self-discipline and courage) were not usually included in the post-graduate courses CONCLUSIONS: The nurse administrator skills should be related to relational and ethical behaviour. The training offer of the post-graduate courses must be reoriented.

Basin characteristics and nutrient losses: the EUROHARP catchment network perspective.

The EC-funded EUROHARP project studies the harmonisation of modelling tools to quantify nutrient losses from diffuse sources. This paper describes a set of study areas used in the project from geographical conditions, to land use and land management, geological and hydro-geological perspectives. The status of data availability throughout Europe in relation to the modelling requirements is presented. The relationships between the catchment characteristics and the nutrient export are investigated, using simple data available for all the catchments. In addition, this study also analyses the hydrological representativity of the time series utilised in the EUROHARP project.

[Complications in cochlear implantation]. / Complicaciones en la implantación coclear.

OBJECTIVE: The objective [corrected] of this paper is to present the surgical complications in cochlear implant, in a serie of 346 patients, submitted to surgery by the same surgical team with the subsequent control and follow-up for a long period of time. We show the description and the handling of each complication. MATERIAL AND METHODS: A follow-up was carried out on 346 cochlear implant patients by the same surgical team, from February 1993 to March 2004. The complications were assessed and their handling and follow-up is reported. The series includes 211 children and 135 adults. RESULTS: The complication rate was 9.8% (n = 34). The complications found were: Intraoperatory Complications: Cerebrospinal Fluid Leaks (CSF), 7; Facial nerve injury, 1; excessive thinning of the posterior wall, 2; inmediate post operatory complications: infection of the surgical wound, 4; post operatory persistent pain, 1; Tinnitus, 2; late complications: Mastoiditis, 2; extrusion of the stimulating receptor, 1; facial nerve stimulation, 5; late infections of the stimulation receptor, 2; technical failure of the implanted system, 7. Neither alteration or migration was found in the receptor placing. CONCLUSIONS: The cochlear implant surgery has a low morbility. We found no cases of meningitis due to this procedure.

Computed tomographic anatomy of the head of the loggerhead sea turtle (Caretta caretta).

The heads of three loggerhead sea turtles were disarticulated and imaged immediately to minimize postmortem changes and then frozen and sectioned. For computed tomography (CT) imaging, the heads were positioned in ventral recumbency. Transverse CT images with soft-tissue window were obtained from the olfactory sac region to the temporomandibular joint region. After CT imaging, the heads were sectioned and the gross sections were compared to CT images, to assist in the accurate identification of the anatomic structures. Different clinically relevant anatomic structures were identified and labelled in two series of photographs (CT images and anatomic cross-sections). CT images provided good differentiation between the bones and the soft tissues of the head. The information presented in this paper should serve as an initial reference to evaluate CT images of the head of the loggerhead sea turtle and to assist in the interpretation of lesions of this region.

[Use of topical ciprofloxacin in chronic suppurating otitis media]. / Utilización del ciprofloxacino tópico en la otitis media crónica supurada.

OBJECTIVE: A prospective study is presented. We carry out an analysis of the results obtained after treatment with different protocols of administration of ciprofloxacin, during the active phase in chronic otitis media and in chronic otorrhea. MATERIAL AND METHOD: A multicenter, prospective study is carried out, in 3 ENT departments, corresponding to 3 reference tertiary hospitals. 300 patients were included ranging from 5 to 73 years old, all were diagnosed of chronic disease of the middle ear: simple chronic otitis media (n = 128), chronic otitis with bone reabsorption (n = 57), cholesteatoma infection (n = 42) and postsurgery ear infection (n = 73). Patients were placed in 5 treatment groups: ciprofloxacin (oral administration) (only adults were included), topical ciprofloxacin (0.5%), topical ciprofloxacin (0.2%), topical ciprofloxacin (0.2%) plus oral ciprofloxacin and ciprofloxacin (0.3%) plus topical fluocinolone. There was a control group treated with polimixin plus neomicine and hidrocortison. RESULTS: The most common isolated bacterias were: Pseudomonas aeruginosa and S. aureus. We found 19 resistant strains to ciprofloxacin. A better therapeutic response was observed in the topical administration groups. In topical administration, a difference was only observed in the cholesteatoma and chronic middle ear infection with bone reabsorption groups, in those patients that were administered the ciprofloxacin with fluocinolone. CONCLUSION: Forms of topical treatment, with ciprofloxacin, in active infection of chronic disease of the middle ear, improve the results compared to oral administration. The association with of topical fluocinolone improves the results in the cases with cholesteatoma infection and chronic middle ear infection.

Design and antileishmanial activity of amphotericin B-loaded stable ionic amphiphile biovector formulations.

A new delivery system, Ionic Amphiphilic Biovector (ABV), comprised of anionic lipids (dipalmitoyl phosphatidyl glycerol) included in a cationic cross-linked polysaccharide matrix was used as a reservoir for amphotericin B (AmB). Two ABV formulations exhibited an in vitro and in vivo efficacy similar to commercial AmBisome against Leishmania donovani-infected mice. The higher stability of these ABV formulations indicates their potential for further development and applications.

Proofs of the structure of lipid coated nanoparticles (SMBV) used as drug carriers.

PURPOSE: Supramolecular Biovectors (SMBV) consist of cross-linked cationic nanoparticles surrounded by a lipid membrane. The purpose was to study the structure of the lipid membrane and to characterise its interaction with the nanoparticles in order to differentiate SMBV from other polymer/lipid associations. METHODS: The interaction of lipids with the nanoparticle surface was studied using zeta potential. Fluorescence Energy Transfer (FET) and Fluorescence Microscopy. SMBV were compared to liposomes and mixtures nanoparticles/liposomes. Finally the structure of SMBV was visualised by Electron Microscopy. RESULTS: Zeta potential measurements showed that lipids on SMBV had a pronounced shielding effect on the surface charge. This was not the case for mixtures of nanoparticles and liposomes. FET experiments confirmed these results indicating that, for SMBV, the lipids are much closer to the nanoparticle surface. SMBV Fluorescence microscopy on model microparticles showed a lipid crown on SMBV that was confirmed by electron microscopy on SMBV nanoparticles. CONCLUSIONS: Results show that in case of SMBV lipids are strongly adsorbed on the polysaccharide core surface probably due to ionic/hydrophobic interactions. The resulting supramolecular structure is a spherical cationic polysaccharide particle surrounded by a phospholipid/cholesterol layer.

[Resistance to antibiotics in clinical isolates of the genus Enterococcus]. / Resistencia a antibióticos en aislamientos clínicos del género Enterococcus.

The purpose of our study was to determine the prevalence of resistance in 114 clinical strains of enterococci. Identification was made using PASCO dehydrated panels and by the conventional method of Facklam and found the following: 92.1% E. faecalis, 7% E. faecium and 0.9% E. avium. The antibiotic susceptibility was determined by the PASCO system, and the minimum inhibitory concentrations of vancomycin, teicoplanin, gentamicin and streptomycin were determined by the agar dilution method. All the strains were susceptible to vancomycin and teicoplanin. We detected resistance to ampicillin in 4.4% of isolates, to penicillin in 6%, to tetracycline in 41.2%, to rifampicin in 12.3%, to chloramphenicol in 23.7%, to erythromycin in 66.7%, to ciprofloxacin in 53.5%, to gentamicin to a high level in 43.9%, and to streptomycin to a high level in 57%. E. faecium was associated with high-level resistance to gentamicin. E. faecium was more resistant than E. faecalis to quinolones, rifampicin, penicillin and ampicillin, and showed similar susceptibility to tetracycline and chloramphenicol. However, E. faecalis was more resistant than E. faecium to aminoglycosides, mainly to gentamicin.

[Keratitis caused by Acanthamoeba in patients with contact lenses]. / Queratitis producida por Acanthamoeba en pacientes portadores de lentes de contacto.

BACKGROUND: Keratitis by Acanthamoeba is a severe infectious complication which may be derived from the use and bad preservation of contact lens. This disease la increasingly more frequent and rapid diagnosis and treatment condition the posterior evolution of the disease. PATIENTS AND METHODS: The cases of 2 contact lens waters who developed keratitis by Acanthamoeba are presented. The diagnostic methods and treatment are commented upon. RESULTS: Keratitis by Acanthamoeba was diagnosed in 2 patients following analysis of corneal scrapings and of the saline solution used for lens maintenance. Trophozoites and cystes of this parasite were observed in all the samples processed. The evolution was good in the patient treated at 15 days after initiation of the symptoms. However, the evolution was very bad in the patient who delayed in initiating the amebicide treatment. Penetrating keratoplasty was performed in both patients. CONCLUSIONS: Specific treatment with derivates of propamidine implemented early may avoid severe ocular complications. As prophylaxis for contact lens users, it is recommended that the contact lens be maintained clean and correctly care for, and the use of home-made saline solutions which are easily contaminated by Acanthamoeba, should be avoided.

[Corynebacterium amycolatum: sepsis in hematologic patients]. / Corynebacterium amycolatum: sepsis en pacientes hematológicos.

BACKGROUND: To evaluate clinical and microbiological characteristics of sepsis due to Corynebacterium amycolatum, because few cases have been reported in human infections by this bacteria. METHODS: We report two cases of sepsis due to Corynebacterium amycolatum, the patients were diagnosed as having leukaemia. The identification procedures are discussed. RESULTS: We describe 2 patients with sepsis in neutropenic phase. Antibiotic treatment was successful in both cases. Clinical isolates were classified as Corynebacterium xerosis by the Api Coryne system Version 1.0. The chemotaxonomic characteristics of the isolates were determined by thin-layer chromatographic analysis. Isolates did not contain mycolic acids, they were identified as Corynebacterium amycolatum. CONCLUSIONS: Sepsis due to Corynebacterium amycolatum is a rare disorder. A test for mycolic acid could be sufficient to make a distinction between Corynebacterium amycolatum and other diphteroid bacteria. Such a test involves thin-layer chromatography.

Proton production and consumption pathways in yeast metabolism. A chemostat culture analysis.

In this investigation, a method for the accurate quantitative determination of net proton production or consumption in biological cultures has been devised. Cells are cultured under constant pH conditions. The specific rate of proton production or consumption by the culture (qH+, mmol h-1 per g biomass) is proportional to the mmol of base or acid required to maintain constant pH per unit time, and this equivalence is independent of the buffering capacity of the culture medium. The above method has been applied to chemostat cultures of Candida utilis growing on glucose or glycerol as carbon source, and different nitrogen sources. The results indicate that the nitrogen assimilation pathway alone determines the value of qH+, and a fixed stoichiometric relationship between nitrogen uptake rate qN (meq h-1 per g biomass) and qH+ has been found for each nitrogen source employed. Thus, qH+/qN values of +1, 0 and -1 were found for ammonium ions, urea and nitrate respectively. Under oxidative metabolism, the contribution of carbon catabolism to the value of qH+ was undetectable. Sine qN may be related to growth and production of type 1 compounds in fermentation processes, the parameter qH+ was incorporated into a model of growth and energy metabolism in chemostat culture (Castrillo and Ugalde, Yeast 10, 185 - 197, 1994), resulting in adequate simulations of experimentally observed culture performance. Thus, it is suggested that qH+ may be employed as a simple and effective control parameter for biotechnological processes involving biomass-related products.

Synthesis and characterization of supramolecular biovector (SMBV) specifically designed for the entrapment of ionic molecules.

Supramolecular biovectors (SMBV) are nanoparticular drug carriers composed of an internal crosslinked solid core externally grafted with fatty acids and surrounded with a phospholipid layer. We show in this paper that the internal core can be derivatized with anionic ligands such as phosphate in order to allow the efficient entrapment of cationic molecules through a process akin to ion exchange. Synthesis of SMBV involved first a cross linking and derivatization step of polysaccharides followed by a homogenization, a drying and a regioselective acylation step. Acylated polysaccharide cores are thus obtained which can be loaded with drugs and wrapped with a phospholipid layer. The SMBVs obtained are characterized through their size, 20 nm, and their ability to filter through 0.22 microns pore size membrane. Gel permeation chromatography experiments performed with various phospholipid/acylated cores ratios indicate that SMBVs form entities distinct from liposomes and that the optimum phospholipid/acylated cores ratio for this specific type of SMBVs is close to 100%. The supramolecular structure of SMBVs and in particular the spatial proximity between acylated cores and phospholipids is demonstrated through resonance energy transfer experiments. The drug loading capability of SMBVs is illustrated by the preparation of gentamicin and doxorubicin loaded SMBV. The therapeutic potential of SMBVs is then discussed notably in the light of a possible biomimetism with low density lipoproteins (LDL).

[Importance of anaerobic bacteria in recurrent childhood non-streptococcal tonsillitis]. / Importancia de las bacterias anaerobias en la amigdalitis recurrente infantil no estreptocócica.

BACKGROUND: The presence of anaerobes in the oropharyngeal flora was already known, but they were infrequently involved in infections, except for specific diseases (abscesses, Vincent's angina). The involvement of anaerobes in more frequent by observed oropharyngeal diseases has been recently proposed. METHODS: We analyzed the tonsillar flora of 22 healthy children (control group) and 54 children diagnosed of recurrent tonsillitis. We compare the clinical response of children with recurrent tonsillitis to two treatments: penicillin, and penicillin plus metronidazole. RESULTS: We isolated Streptococcus pyogenes from 22.2% of children with tonsillitis, and did not isolate it from any healthy children. The rest of tonsillar flora was qualitatively similar in both groups. Both populations showed a high proportion of beta-lactamase-producer bacteria. In children from whom S. pyogenes was isolated, the response to the treatment was similar for both treatments. In those cases where S. pyogenes was not isolated, penicillin + metronidazole cured the 77.2% of children treated, but penicillin cured only 30% of cases. CONCLUSIONS: These results suggest that anaerobes might be involved, at least, in recurrent tonsillitis when S. pyogenes is not found, and justify further studies about the etiology and treatment of this disease.

Use of mixed perfluorinated ion-pairing agents as solvents in ion-pair high-performance liquid chromatography for the preparative purification of aminoglycoside antibiotics.

The possibility of using mixed ion-pair reversed-phase high-performance liquid chromatography for the preparative purification of aminoglycoside antibiotics was investigated. Retention can be satisfactorily defined in aqueous conditions but efficiency is critical. The analysis of a polynominal model allowed ion-pairing agents compositions to be designed leading to improved efficiency. Inexpensive aqueous mobile phases using volatile buffers can be defined for the satisfactory ion-pair preparative chromatography of aminoglycosides.