Green tea and glycine aid in the recovery of tendinitis of the Achilles tendon of rats.

PURPOSE: Green tea (GT) is widely used due to its anti-inflammatory properties. Previous studies have shown beneficial effects of a glycine diet on the remodeling process in inflamed tendons. Tendinitis is commonly observed in athletes and is of concern to surgeons due to the slowness of the recovery process. Our hypothesis is that GT + a glycine diet may improve tendinitis. AIM OF THE STUDY: To analyze the effect of GT and/or glycine in the diet on tendinitis. MATERIALS AND METHODS: Wistar rats were divided into seven groups (G): control group (C); G1 and G4, tendinitis; G2 and G5, tendinitis supplied with GT; and G3 and G6, tendinitis supplied with GT and a glycine diet for 7 or 21 days, respectively. We performed zymography for metalloproteinase, biochemical, morphological and biomechanics tests. RESULTS: G2, G3 and G5 showed high levels of hydroxyproline in relation to G1, while G4 showed high levels of glycosaminoglycans. High activity of metalloproteinase-2 was detected in G3. The organization of collagen bundles was better in G2 and G3. G5 showed similar birefringence measurements compared with C. G5 withstood a larger load compared with G4. CONCLUSIONS: The presence of metalloproteinase-2 indicates that a tissue is undergoing a remodeling process. High birefringence suggests a better organization of collagen bundles. After 21 days, G5 sustained a high load before rupture, unlike G4. The results suggest that GT + a glycine diet has beneficial effects that aid in the recovery process of the tendon after tendinitis.

Structural and biomechanical changes in the Achilles tendon after chronic treatment with statins.

Cases of tendinopathy and tendon ruptures have been reported as side effects associated with statin therapy. This work assessed possible changes in the structural and biomechanical properties of the tendons after chronic treatment with statins. Wistar rats were divided into the following groups: treated with atorvastatin (A-20 and A-80), simvastatin (S-20 and S-80) and the group that received no treatment (C). The doses of statins were calculated using allometric scaling, based on the doses of 80 mg/day and 20 mg/day recommended for humans. The morphological aspect of the tendons in A-20, S-20 and S-80 presented signals consistent with degeneration. Both the groups A-80 and S-80 showed a less pronounced metachromasia in the compression region of the tendons. Measurements of birefringence showed that A-20, A-80 and S-80 groups had a lower degree of organization of the collagen fibers. In all of the groups treated with statins, the thickness of the epitenon was thinner when compared to the C group. In the biomechanical tests the tendons of the groups A-20, A-80 and S-20 were less resistant to rupture. Therefore, statins affected the organization of the collagen fibers and decreased the biomechanical strength of the tendons, making them more predisposed to ruptures.

Courtship behavior of Zaprionus indianus (Gupta) (Diptera: Drosophilidae) from populations colonizing South America.

We describe for the first time the sexual behavior and the courtship song of males of the African fly Zaprionus indianus (Gupta), a recent invader of South America. The male courtship song is formed by monocyclic pulses and the courtship behavior is simple when compared to that of species of Drosophila. Two interpulse interval (IPI) distributions were observed: pre-mounting and mounting. No significant difference was observed between the pre-mounting IPIs of males that descended from three geographical populations from South America. We also observed the songs produced by females and the homosexual behavior exhibited by males. A sequence of bursts is produced by females as a refusal signal against males, while males emit a characteristic song that identifies sex genus, which differs from the courtship song. The short courtship and mating latencies recorded reveal vigorous males and receptive females, respectively.

General discrete modelling of lung sound production in normal subjects.

Lung sound analysis is of a major importance in diagnostic malfunctions of the respiratory system. In normal subjects, it is known that these sounds are caused by the interaction of the respiratory flows with the bronchial tree structure. However, the detailed knowledge of the reasons for the spectral characteristics of such sounds remains to be elucidated. In this paper we propose a model for normal lung sound production based on a discretization of air flow in particle-like elements. Their transport with the involved interactions is implemented using a pseudo-molecular dynamics Monte Carlo procedure. General physical principles were considered for the interaction of these elements with the bronchial tree as well as a two-body interaction potential. The particle-tree interactions and the particle-particle interactions represent the flow-tree and the internal flow interactions, respectively. According to the model, sound is produced in each bronchus with the pitch frequency inversely proportional to its dimensions and with amplitude proportional to the intensity of the interaction, also a function of the bronchus dimensions. The lung sound is then the composition of the sounds produced in each bronchus. The model was successful in approximating the spectral characteristics reported by Gavriely et al (1981, 1995) as a direct consequence of the fractal properties of the bronchial tree and the considered internal fluid interactions. Thus, the reported high-frequency spectrum with its affine property as well as the low-frequency irregularity could be reproduced.

General fractal-discrete scheme for high-frequency lung sound production.

A general scheme is proposed to explain the observed spectral properties of high-frequency human respiratory sounds in terms of the interaction between the respiratory flux and a bronchial tree of fractal properties. The air flux is treated as composed of discrete decoupled elements while the tree is assumed to have a Cantor-based geometry. According to this model, the affine behavior often observed in the high-frequency (log-log) spectral range is a direct consequence of the fractal geometry of the bronchial tree in both qualitative and quantitative aspects. This strongly indicates that the dynamics underlying the high-frequency sound generation must have at most nondominant couplings between the relevant fluid components.