Effect of multiple stressors and population decline of frogs.

The ongoing decline in anuran populations is linked primarily to the effects of stressor agents such as pathogens, pesticides, alterations of natural landscapes, and the introduction of exotic species. Most studies that have evaluated the effects of these stressors have focused on a single component, which is the opposite of the reality of most natural environments, where anuran populations tend to suffer the influence of multiple agents simultaneously. Studies of the effects of the interaction between these components are extremely important, given that one agent may potentialize (synergistic effect) or weaken another (antagonistic effect) or, in some cases, have a neutral effect. The present study is based on the scientometric analysis of three bibliographic databases (ISI Web of Science, Scopus, and PubMed), which identified 1376 papers that reported on the global decline of anuran populations, although only 172 of these studies focused on the interactive effects of environmental stressors. Synergistic effects were the most frequent type of interaction, followed by antagonistic effects, and a small number of studies that found no clear interaction between the stressors. Pathogens and pesticides were the classes of stressor studied most frequently, while climate-pathogen and pathogen-pesticide interactions were the combinations that featured in the largest number of studies. Overall, we would recommend a more systematic focus on the dynamics of the interactions among the stressors that impact anuran populations, in particular for the elaboration of conservation programs, given that these agents tend to have complex combined effects.

Mutagenic, genotoxic and morphotoxic potential of different pesticides in the erythrocytes of Podocnemis expansa neonates.

Despite the damaging effects of pesticides glyphosate (Gly), atrazine (Atra) and fipronil (Fip) on different organisms, the mutagenic, genotoxic and morphotoxic potential of testudine erythrocytes in freshwater remains unknown. Thus, the aim of the present study is to assess the toxicological potential of these compounds in Podocnemis expansa (Amazonian turtles) neonates from eggs artificially incubated in substrate at different concentrations of herbicides Gly and Atra and insecticide Fip. Micronucleus test and other nuclear abnormalities, as well as comet assay and morphometric measurements taken of models' circulating erythrocytes were used as toxicity biomarkers. Pups exposed to Gly (groups Gly-65 ppb and Gly-6500 ppb) were the ones recording the largest amount of nuclear abnormalities; erythrocytes with multilobulated, notched and displaced nucleus were mostly frequent in groups Atra-2 ppb and Gly -65 ppb. All treatments (Gly-6500 ppb, Atra-2 ppb, Atra-200 ppb, Fip-4 ppb and Fip-400 ppb), except for group Gly-65 ppb, led to decreased erythrocyte area, increased "nuclear area: erythrocyte area" ratio, as well as to decreased erythrocyte and erythrocyte nuclei circularity, which highlights the clear effect on the size and shape of these cells. On the other hand, the comet assay did not evidence any genotoxic effect caused by the assessed pesticides. This is a pioneer study on the mutagenic and morphotoxic potential of pesticides in P. expansa eclodides exposed in ovo to Gly, Atra and Fip; therefore, it is an insight on how these compounds can affect the health of these animals.

Current Status of Ecotoxicological Studies of Bats in Brazil.

Bats are sensitive to contaminants generated by agricultural activities, mining, and urbanization. In this review, we update the status of bat toxicology in Brazil. Agriculture, for example, in addition to habitat fragmentation and loss, undoubtedly affects non-target organisms through the use of pesticides. Other factors such as trace metals are a neglected problem in the country, as they can deposit on insects and plants reaching bats through the ingestion of these foods. Of the 184 species of bats in the country, only 4.9% have been investigated. The frugivorous species, Artibeus lituratus, has frequently been studied for the effects of pesticide exposure, and impacts at the cellular level on metabolism and reproduction have been observed. Given the scarcity of studies on bat ecotoxicology, we encourage national researchers and scientists elsewhere to increase knowledge of the effects of chemical contaminants on bats in Brazil.