RESUMO
The P22 ELISA was recently developed for the serodiagnosis of animal tuberculosis. Herein, the stability of the P22 antigen in different presentations and storage conditions, and the cross-reactivity with Corynebacterium pseudotuberculosis infection in small ruminants were evaluated. For the stability assay, serum samples from cows, sheep, goats, alpacas, badgers, and wild boar were used in the P22 ELISA. The cross-reactivity analysis used sera from sheep and goats with caseous lymphadenitis (CLA). Differences in the immune recognition of P22 were found when the antigen was stored at 40 °C, but without altering the negative or positive status of each sample. P22 ELISA presented 5.71 % cross-reactivity when CLA-positive sheep were evaluated, but no cross-reaction was observed among CLA-positive goat serum samples. This study showed that the P22 protein complex is stable under different formulations and temperatures, and that the assay presents a low cross-reactivity with CLA.
Assuntos
Doenças dos Bovinos , Infecções por Corynebacterium , Corynebacterium pseudotuberculosis , Doenças das Cabras , Doenças dos Ovinos , Tuberculose , Feminino , Ovinos , Bovinos , Animais , Doenças das Cabras/microbiologia , Doenças dos Ovinos/microbiologia , Infecções por Corynebacterium/diagnóstico , Infecções por Corynebacterium/veterinária , Infecções por Corynebacterium/microbiologia , Cabras/microbiologia , Testes Sorológicos/veterinária , Tuberculose/veterináriaRESUMO
The main etiologic agent of tuberculosis (TB) in livestock is Mycobacterium bovis; human TB cases caused by M. bovis are rare. Analysis of a TB outbreak caused by polyresistant M. bovis involving a human and sympatric sheep in Spain suggests local circulation of drug-resistant M. bovis strains among livestock.
