RESUMO
The effect of lipoprotein-deficient serum (LPDS) with and without added low-density lipoprotein (LDL), isolated from diabetic subjects, on the replication of SV40-transformed islet cells (HIT cells) was investigated. Whole serum as well as LPDS preparations stimulated DNA synthesis maximally when added to the culture medium at a final concentration of 0.1%. The addition of LDL at 25 and 175 micrograms protein/ml medium did not cause further stimulation. On the contrary, the higher concentrations resulted in a significant inhibition. These results suggest that previously observed stimulation of DNA synthesis in smooth muscle cells by LDL from diabetic subjects is most likely due to the presence of growth factors in the serum of these patients and not to LDL per se.
Assuntos
DNA/biossíntese , Diabetes Mellitus Tipo 2/sangue , Ilhotas Pancreáticas/patologia , Lipoproteínas LDL/fisiologia , Fenômenos Fisiológicos Sanguíneos , Divisão Celular/fisiologia , Linhagem Celular Transformada , Humanos , Insulina/metabolismo , Secreção de Insulina , Ilhotas Pancreáticas/metabolismoRESUMO
The effect of the calcium-blockers nicardipine, darodipine, PN-200-110 on insulin release from pancreatic islets was studied using nifedipine as a reference compound. All drugs at a concentration of 10(-6)M significantly inhibited insulin release in response to both low (5.5 mM) and high (22 mM) glucose. The present observations support previous reports that calcium blockers of the dihydropyridine series are effective inhibitors of glucose-induced insulin release.