Assuntos
Ossos Faciais/anormalidades , Crânio/anormalidades , Anormalidades Induzidas por Medicamentos/etiologia , Anormalidades Induzidas por Medicamentos/prevenção & controle , Anormalidades Múltiplas/etiologia , Anormalidades Múltiplas/prevenção & controle , Animais , Feminino , Hidrazinas/toxicidade , Masculino , Camundongos , Camundongos Endogâmicos , Ratos , Ratos EndogâmicosRESUMO
The effect of several hydroxamic acids on cell growth and differentiation was studied in vitro in cultures of Friend erythroleukemia cells, line F4-6. Terminal differentiation in F4-6 cells can be induced by exposure to a variety of structurally unrelated compounds or to conditions which inhibit cell growth. Hydroxamic acids do not induce erythroid differentiation but interfere with both cell growth of F4-6 cells and the induction of differentiation by DMSO in these cells. DMSO-induced terminal differentiation is inhibited even when F4-6 cells are pretreated for 24 h with hydroxamates followed by removal of the hydroxamates and transfer to fresh medium containing 1% DMSO. Reduction of cell growth by hydroxamates is completely and immediately reversible upon removal. In contrast, the inhibition of DMSO inducibility is not reversible within 24 h. Cell pretreated with hydroxamates for 24 h prior to a 96 h-exposure to DMSO show the same reduction in synthesis of hemoglobin as cells simultaneously exposed to DMSO and hydroxamates.
Assuntos
Diferenciação Celular/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Ácidos Hidroxâmicos/farmacologia , Leucemia Eritroblástica Aguda/patologia , Animais , Dimetil Sulfóxido/antagonistas & inibidores , Eritrócitos/efeitos dos fármacos , Eritrócitos/fisiologia , CamundongosAssuntos
Hormônios/efeitos adversos , Gravidez/efeitos dos fármacos , Animais , Feminino , Humanos , Camundongos , RatosRESUMO
The DL50 of escin was determined after i.p. application. Juvenile male rats were treated with 2X5 mg/kg escin at age 32 days. After they had reached fertility, kidneys, testes and sperm were examined. The high dose of escin used did not affect fertility and a nephrotoxic activity could not be detected either.
Assuntos
Escina/farmacologia , Fertilidade/efeitos dos fármacos , Saponinas/farmacologia , Fatores Etários , Animais , Escina/toxicidade , Rim/efeitos dos fármacos , Dose Letal Mediana , Masculino , Ratos , Espermatozoides/efeitos dos fármacos , Testículo/efeitos dos fármacosAssuntos
Anormalidades Induzidas por Medicamentos/prevenção & controle , Fenda Labial/prevenção & controle , Fissura Palatina/prevenção & controle , Embrião de Mamíferos/efeitos dos fármacos , Ácidos Hidroxâmicos/efeitos adversos , Animais , Bovinos , Fenômenos Químicos , Química , Fenda Labial/induzido quimicamente , Fenda Labial/embriologia , Fissura Palatina/induzido quimicamente , Modelos Animais de Doenças , Feminino , Idade Gestacional , Heterozigoto , Homozigoto , Humanos , Hidroxilação , Gravidez , Ratos , Extratos de Tecidos/administração & dosagem , Complexo Vitamínico B/administração & dosagem , Vitaminas/administração & dosagemRESUMO
Principles of teratogenic and mutagenic actions are defined. The recent experimental studies with laboratory animals, and our investigations with caffeine-sodium benzoicum and with soluble coffee in pregnant rats and mice showed no teratogenicity. The results are compared with specific teratogenic effects of cytostatic agents. A teratogenicity of caffeine can be excluded, a mutagenicity in animal experiments can also not be proved.