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1.
Lasers Med Sci ; 36(4): 791-802, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32638240

RESUMO

Compensatory hypertrophy (CH) occurs due to excessive mechanical load on a muscle, promoting an increase in the size of muscle fibers. In clinical practice, situations such as partial nerve injuries, denervation, and muscle imbalance caused by trauma to muscles and nerves or diseases that promote the loss of nerve conduction can induce CH in muscle fibers. Photobiomodulation (PBM) has demonstrated beneficial effects on muscle tissue during CH. The aim of the present study was to evaluate the effect of PBM on the inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) as well as type 2 metalloproteinases (MMP-2) during the process of CH due to excessive load on the plantaris muscle in rats. Forty-five Wistar rats weighing 250 g were divided into three groups: control group (n = 10), hypertrophy (H) group (n = 40), and H + PBM group (n = 40). CH was induced through the ablation of synergist muscles of the plantaris muscle. The tendons of the gastrocnemius and soleus muscles were isolated and sectioned to enable the partial removal of each of muscle. The preserved plantaris muscle below the removed muscles was submitted to excessive functional load. PBM was performed with low-level laser (AsGaAl, λ = 780 nm; 40 mW; energy density: 10 J/cm2; 10 s on each point, 8 points; 3.2 J). Animals from each group were euthanized after 7 and 14 days. The plantaris muscles were carefully removed and sent for analysis of the gene and protein expression of IL-6 and TNF-α using qPCR and ELISA, respectively. MMP-2 activity was analyzed using zymography. The results were submitted to statistical analysis (ANOVA + Tukey's test, p < 0.05). The protein expression analysis revealed an increase in IL-6 levels in the H + PBM group compared to the H group and a reduction in the H group compared to the control group. A reduction in TNF-α was found in the H and H + PBM groups compared to the control group at 7 days. The gene expression analysis revealed an increase in IL-6 in the H + PBM group compared to the H group at 14 days as well as an increase in TNF-α in the H + PBM group compared to the H group at 7 days. Increases in MMP-2 were found in the H and H + PBM groups compared to the control group at both 7 and 14 days. Based on findings in the present study, it is concluded that PBM was able to modulate pro-inflammatory cytokines that are essential for the compensatory hypertrophy process. However, it has not shown a modulation effect directly in MMP-2 activity during the same period evaluated.


Assuntos
Citocinas/metabolismo , Regulação da Expressão Gênica/efeitos da radiação , Terapia com Luz de Baixa Intensidade , Músculo Esquelético/patologia , Músculo Esquelético/efeitos da radiação , Animais , Hipertrofia/metabolismo , Hipertrofia/patologia , Hipertrofia/radioterapia , Interleucina-6/metabolismo , Masculino , Metaloproteinase 2 da Matriz/metabolismo , Músculo Esquelético/metabolismo , Ratos , Ratos Wistar , Tendões/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
2.
Lasers Med Sci ; 35(4): 777-788, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31768691

RESUMO

To date, there is no compilation of evidence-based information associating photobiomodulation effect and salivary glands. This systematic review aims to assess photobiomodulation effect of low intensity laser on salivary glands in the presence of systemic diseases. MEDLINE databases were searched in duplicate through December 2018. In vivo studies and clinical trials were included if photobiomodulation was performed in salivary glands of animal (rat or mice) or human in the presence of systemic disease. The methodological quality was assessed in duplicate using the modified Newcastle-Ottawa scale (NOS). Search strategy identified 483 potentially eligible articles, and 449 were included. The Boolean search naturally leads to a high amount of works the majority of which were excluded because the analysis of the title and abstract demonstrated it was not focusing on PBM. Only 34 studies were selected for the full-text analysis, of which 5 were excluded due to non-use of photobiomodulation, 4 due to lack of control group, 2 because they were studies of cell cultures and 1 because they did not have the total of animals used. Thus, 21 papers were included for the critical evaluation of the impact of photobiomodulation on the major salivary glands; the studies used rats (n = 10) and humans (n = 11). Although studies reported an increase in the salivary rate, decrease in pain, and increase in quality of life after the PBM, the lack of standards for the application of light and reporting of the parameters, make it hard to reproduce the results. This topic is still in need for further research.


Assuntos
Terapia com Luz de Baixa Intensidade , Glândulas Salivares/efeitos da radiação , Animais , Ensaios Clínicos como Assunto , Humanos , Camundongos , Dor/radioterapia , Qualidade de Vida , Ratos
3.
Lasers Med Sci ; 32(9): 2155-2165, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29063472

RESUMO

Phototherapy has demonstrated positive effects in the treatment of peripheral nerve injury, but there is a need to investigate the dosimetric parameters. Thus, the aim of the present study was to conduct a literature review on the effects of photobiomodulation with the use of low-level laser therapy (LLLT) on the treatment of peripheral nerve injury in experimental models. The databases of PubMed/MEDLINE, SCOPUS, and SPIE Digital Library were searched for articles on the use of LLLT in experimental models of peripheral nerve injury published in English between January 2007 and March 2016. The laser parameter variability was wavelength (632.8 to 980 nm), power (10 to 190 mW), and total energy (0.15 to 90 J) in pulsed or continuous wave and single or multiple points. Eighteen original articles demonstrating the effects of LLLT on the acceleration of functional recovery, morphological aspects as well as the modulation of the expression inflammatory cytokines, and growth factors were selected. LLLT is a viable phototherapeutic modality for the treatment of peripheral nerve injury, demonstrating positive effects on the neuromuscular repair process using either red or infrared light. The majority of studies used a power of up to 50 mW and total energy of up to 15 J administered to multiple points. The determination of these parameters is important to the standardization of a LLLT protocol to enhance the regeneration process following a peripheral nerve injury.


Assuntos
Terapia com Luz de Baixa Intensidade/métodos , Traumatismos dos Nervos Periféricos/radioterapia , Animais , Modelos Animais de Doenças , Regeneração Nervosa/efeitos da radiação , Recuperação de Função Fisiológica
4.
Endocr Regul ; 48(3): 152-8, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25110214

RESUMO

The search for a more efficient repair process of muscle injuries has become evident in clinical practice. The aim of the present study was to evaluate the effect of nandrolone decanoate (ND) on the proliferation, adhesion, and expression of myogenic regulatory factors (MRFs) in C2C12 cells.Methods. Cell proliferation and adhesion were assessed using an MTT assay. The expression of MRFs was assessed by real-time PCR.Results. ND applied at 10 or 25 µM concentration induced after 60 min an increase in adhesion, at 5 µM concentration induced after 5 days an increase in cell proliferation, and ND at 50 µM concentration led after 5 days to a decrease in cell proliferation in comparison with other groups. The steroid did not alter the expression of MRFs.Conclusions. The positive effects of ND regarding the proliferation and adhesion of C2C12 cells suggest that this steroid may have positive effects following a muscle injury.


Assuntos
Proliferação de Células/efeitos dos fármacos , Mioblastos/efeitos dos fármacos , Nandrolona/análogos & derivados , Animais , Adesão Celular/efeitos dos fármacos , Adesão Celular/genética , Proliferação de Células/genética , Células Cultivadas , Regulação da Expressão Gênica/efeitos dos fármacos , Camundongos , Desenvolvimento Muscular/efeitos dos fármacos , Desenvolvimento Muscular/genética , Proteína MyoD/genética , Proteína MyoD/metabolismo , Mioblastos/fisiologia , Miogenina/genética , Miogenina/metabolismo , Nandrolona/farmacologia , Decanoato de Nandrolona , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
5.
J Clin Pediatr Dent ; 39(1): 27-9, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25631722

RESUMO

The aim of the present study was to describe a clinical protocol for the treatment of white spots with the use of an abrasive material. A four-year-old patient presented with a white spot on tooth 51 and a white spot associated with a carious lesion in the cervical region of tooth 52. Treatment was planned with microabrasion and restoration of the upper right lateral incisor. Prophylaxis was first performed, followed by protection with a dental dam and the application of the abrasive material (silicon carbide and hydrochloric acid 6%). Five applications were needed to remove the spots. The restoration of the upper right lateral incisor was then performed with a resin composite. A good esthetic outcome was achieved and both the patient and her guardians were satisfied with the results. Microabrasion is a conservative treatment option that achieves satisfactory results with regard to tooth color.


Assuntos
Cárie Dentária/terapia , Microabrasão do Esmalte/métodos , Compostos Inorgânicos de Carbono/uso terapêutico , Pré-Escolar , Protocolos Clínicos , Resinas Compostas/química , Materiais Dentários/química , Profilaxia Dentária , Restauração Dentária Permanente/instrumentação , Restauração Dentária Permanente/métodos , Estética Dentária , Humanos , Ácido Clorídrico/uso terapêutico , Incisivo/patologia , Masculino , Diques de Borracha , Compostos de Silício/uso terapêutico , Colo do Dente/patologia
6.
Lasers Med Sci ; 29(2): 813-21, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23982721

RESUMO

The treatment of muscle injuries is a common practice at rehabilitation centers. Low-level laser therapy (LLLT) has demonstrated positive effects regarding the modulation of the inflammatory response, the enhancement of the tissue repair process and the prevention of fibrosis. The aim of the present study was to evaluate the effects of LLLT on morphological aspects of muscle tissue, collagen remodeling and activity of matrix metalloproteinase 2 (MMP-2) in rat skeletal muscle following acute injury. Wistar rats were divided into five groups: (1) control group (n = 10), (2) sham group (n = 10), (3) LLLT group (n = 30), (4) non-treated injury group (n = 30) and (5) injury + LLLT group (n = 30). Cryoinjury was performed on the belly of the tibialis anterior (TA) muscle. LLLT was performed daily with an AlGaAs laser (780 nm; beam spot of 0.04 cm(2), output power of 40 mW, power density of 1 W/cm(2), energy density of 10 J/cm(2) and 10-s exposure time). Animals were euthanized at 1, 3 and 7 days. The TA muscles were removed and weighed. Morphological aspects were evaluated using H & E staining. The amount and distribution of collagen fibers were evaluated by picrosirius staining. Characterization and activity of MMP-2 were evaluated by zymography and Western blot techniques, respectively. The results revealed that LLLT induced a reduction in inflammatory infiltrate and myonecrosis after 1 day, an increase in the number of blood vessels after 3 and 7 days as well as an increase in the number of immature muscle fibers and MMP-2 gelatinase activity after 7 days. In conclusion, LLLT has a positive effect on the inflammatory process, MMP2 activity and collagen organization and distribution in the repair process of rat skeletal muscle.


Assuntos
Terapia com Luz de Baixa Intensidade/métodos , Músculo Esquelético/lesões , Tíbia/patologia , Animais , Colágeno/metabolismo , Fibrose/radioterapia , Masculino , Metaloproteinase 2 da Matriz/metabolismo , Músculo Esquelético/patologia , Ratos Wistar
7.
Int J Sports Med ; 34(1): 87-2, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22791621

RESUMO

This study analyzed the effect of nandrolone decanoate (ND) on muscle repair and the expression of myogenic regulatory factors following cryoinjury in rat skeletal muscle. Adult male Wistar rats were randomly divided into 4 groups: control group, sham group, cryoinjured group treated with ND and non-injured group treated with ND. Treatment consisted of subcutaneous injections of ND (5 mg/kg) twice a week. After sacrifice, the tibialis anterior muscle was removed for the isolation of total RNA and analysis of myogenic regulatory factors using real-time PCR as well as morphological analysis using the hematoxylin-eosin assay. There was a significant increase in MyoD mRNA after 7 days and in myogenin mRNA after 21 days in the cryoinjured ND group in comparison to other groups in the same period. The morphological analysis revealed no edema or myonecrosis after 7 days as well as no edema or inflammatory infiltrate after 14 days in the cryoinjured ND group. In conclusion the anabolic steroid nandrolone decanoate can modulate the muscle repair process in rats following cryoinjury by influencing the expression of regulatory myogenic factors and phases of muscle repair.


Assuntos
Anabolizantes/farmacologia , Músculo Esquelético/efeitos dos fármacos , Nandrolona/análogos & derivados , Anabolizantes/administração & dosagem , Animais , Modelos Animais de Doenças , Regulação da Expressão Gênica/efeitos dos fármacos , Injeções Subcutâneas , Masculino , Músculo Esquelético/lesões , Músculo Esquelético/patologia , Proteína MyoD/genética , Fatores de Regulação Miogênica/genética , Nandrolona/administração & dosagem , Nandrolona/farmacologia , Decanoato de Nandrolona , RNA/metabolismo , Distribuição Aleatória , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real , Fatores de Tempo
8.
Eur J Paediatr Dent ; 13(2): 97-100, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22762169

RESUMO

AIM: The aim of the present study was to perform a comparative analysis of occlusal contact points in children with and without signs and symptoms of Temporomandibular Disorders (TMD). STUDY DESIGN: Cross-sectional study. One hundred fifty children between 6 and 14 years of age were evaluated using the Helkimo questionnaire and a clinical exam. The occlusal contact points in each child were recorded during maximal intercuspation with the aid of carbon strips. Digital photographs were taken of the upper and lower arches before and after recording the occlusal contacts. The number of contact points between sides were compared and recorded on individual charts (occlusograms). STATISTICS: Student's t-test and Pearson's chi-square test were used for the statistical analysis, with the level of significance set at 0.05, which revealed no statistically significant differences between genders. The Student's t-test revealed a statistically significant difference in the mean number of occlusal contact points between the participants with and without TMD, with a higher number of contact points among those without TMD. There was no significant difference between sides. RESULTS The results of this study show a difference in the number of occlusal contact points in centric occlusion between children with and without TMD. CONCLUSION Regardless of the degree of severity, the number of occlusal contact points is lower among children with TMD.


Assuntos
Oclusão Dentária Central , Registro da Relação Maxilomandibular , Transtornos da Articulação Temporomandibular/patologia , Adolescente , Criança , Estudos Transversais , Feminino , Humanos , Processamento de Imagem Assistida por Computador/métodos , Registro da Relação Maxilomandibular/instrumentação , Masculino , Músculos da Mastigação/patologia , Músculos do Pescoço/patologia , Palpação , Fotografia Dentária/métodos , Projetos Piloto , Amplitude de Movimento Articular/fisiologia , Articulação Temporomandibular/patologia , Transtornos da Articulação Temporomandibular/diagnóstico
9.
J Clin Pediatr Dent ; 35(3): 251-4, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21678665

RESUMO

BACKGROUND: The chemical-mechanical removal of caries involves the chemical softening of the infected dentin, following by the mechanical removal of the softened tissue using non-cutting manual instruments. Papacárie (Fórmula&Açao, Sao Paulo, Brazil) is a product in gel form used for the chemical-mechanical softening of the affected tissue. OBJECTIVE: The purpose of the present study was to evaluate the effectiveness of Papacárie. METHODS: Clinical and radiographic evaluations were performed on 14 young permanent molars treated with Papacárie and restored with glass ionomer cement (VitroMolar DFL Ind. Com LTDA, Rio de Janeiro, Brazil) 24 months following intervention. RESULTS: Success was achieved in 13 of the 14 cases. CONCLUSION: Papacárie was effective in the treatment of carious lesions and constitutes a conservative alternative that offers benefits to patients.


Assuntos
Tratamento Dentário Restaurador sem Trauma/métodos , Cárie Dentária/terapia , Preparo da Cavidade Dentária/métodos , Cimentos de Ionômeros de Vidro/uso terapêutico , Papaína/uso terapêutico , Adolescente , Criança , Cárie Dentária/diagnóstico por imagem , Falha de Restauração Dentária , Restauração Dentária Permanente/métodos , Seguimentos , Géis , Humanos , Dente Molar/diagnóstico por imagem , Estudos Prospectivos , Radiografia , Resultado do Tratamento
10.
Indian J Dent Res ; 20(3): 365-9, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19884725

RESUMO

Inflammation of oral mucosa induced by anti neoplastic drugs is an important, dose limiting and costly side effect of cancer therapy. Here is presented an exacerbated case of oral mucositis associated with renal failure in a patient who underwent bone marrow transplantation. The clinical aspects and an integrated rehabilitation program are discussed below.


Assuntos
Protocolos de Quimioterapia Combinada Antineoplásica/efeitos adversos , Mieloma Múltiplo/complicações , Insuficiência Renal/complicações , Estomatite/etiologia , Condicionamento Pré-Transplante/efeitos adversos , Transplante de Medula Óssea , Assistência Odontológica para Doentes Crônicos/métodos , Feminino , Humanos , Terapia a Laser , Pessoa de Meia-Idade , Mieloma Múltiplo/terapia , Estomatite/terapia , Resultado do Tratamento
11.
J Clin Pediatr Dent ; 32(3): 177-80, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18524264

RESUMO

The chemo-mechanical model of caries removal has been introduced as an alternative method of caries removal and is indicated to overcome the inconvenience of using burs and local anesthesia, causing less discomfort to the patient, and preserving healthy dental structure. Papacarie is a papain gel composed basically of papain, chloramines, and toluidine blue. Papain interacts with exposed collagen by the dissolution of dentin minerals through bacteria, making the infected dentin softer, and allowing its removal with non-cutting instruments without local anesthesia and burs. This paper proposes the use of a papain-based gel for the removal of active caries with infected tissue followed by a glass ionomer restoration in an adolescent patient. Removal of caries tissue with Papacarie proved to be efficient, easy and comfortable for the patient.


Assuntos
Cárie Dentária/terapia , Preparo da Cavidade Dentária/métodos , Papaína/uso terapêutico , Adolescente , Restauração Dentária Permanente , Dentina , Géis , Cimentos de Ionômeros de Vidro , Humanos , Masculino
12.
Oral Dis ; 13(3): 270-3, 2007 May.
Artigo em Inglês | MEDLINE | ID: mdl-17448207

RESUMO

AIM: The aim of the present study was to clinically evaluate the oral mucosa lesions of leprosy patients during and after multi-drug therapy. METHODS: Clinical examination, medical and dental history examination was performed in 100 leprosy patients. RESULTS: The results revealed that 71 patients, 50 men and 21 women, exibited oral lesions. The most frequent lesions were: fissured tongue (18 cases), inflammatory papillary hyperplasia (16 cases), chronic atrophic candidiasis (10 cases), fibroma (10 cases), erythematous candidiasis (eight cases), and traumatic ulceration (seven cases). CONCLUSION: We conclude that leprosy-related lesions are not present in patients undergoing treatment for leprosy, probably due to response to multidrug therapy.


Assuntos
Hanseníase/complicações , Doenças da Boca/etiologia , Idoso , Brasil , Estudos Transversais , Dermatoses Faciais/etiologia , Feminino , Humanos , Hansenostáticos/uso terapêutico , Hanseníase/tratamento farmacológico , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/patologia
13.
Int Endod J ; 40(1): 72-8, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17209836

RESUMO

AIM: To present an additional case of intraosseous schwannoma involving the apical area in the mandibular alveolar bone mimicking an inflammatory periapical lesion. SUMMARY: This article describes a case of schwannoma periapically located mimicking an inflammatory periapical lesion in the mandible of a 34-year-old female. Diagnostic and therapeutic problems can occur when this lesion is misinterpreted as being endodontic in origin. The diagnosis, radiograph, immunohistochemical aspects and treatment are also discussed. KEY LEARNING POINTS: Intraosseous schwannoma is a rare unilocular radiolucency that when located periapically could be misdiagnosed as an endodontic lesion and result in unnecessary root canal treatment. The vitality of the pulp is an important test to exclude lesions of inflammatory origin. Histological examination is important to establish the diagnosis of lesions in the periradicular region.


Assuntos
Neoplasias Mandibulares/patologia , Neurilemoma/patologia , Granuloma Periapical/diagnóstico , Adulto , Diagnóstico Diferencial , Feminino , Humanos , Neoplasias Mandibulares/cirurgia , Neurilemoma/cirurgia
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