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1.
Braz. j. biol ; 83: e244784, 2023. tab, graf
Artigo em Inglês | LILACS-Express | MEDLINE, LILACSEXPRESS | ID: biblio-1278541

RESUMO

Abstract The work aimed to evaluate the weight-length relationship and the condition factor, characterizing the biometry, anatomy, histology and volumetric density of the liver of Brycon amazonicus, in different stages of body growth. The experiment used twenty specimens in four stages of body growth (PI, PII, PIII and PIV) harvested every 90 days, containing five specimens, each group. The livers were dissected, weighed (g) and processed routinely using the hematoxylin and eosin technique. The data were submitted to analysis of variance, Pearson's correlation test and linear regression. The equation that represented the weight-length relation was W = 0.05902 x L2.63, with negative allometric growth, but with a relative condition factor equal to 1.0. The liver was divided into three lobes with the gallbladder close to the right lobe and its color varied from light red to dark red, not varying in relation to other fish species. The hepatosomatic relationship followed body growth until the PII group stage and then declined, demonstrating the behavior of its development in Brycon amazonicus. The organ consists predominantly of hepatocytes, followed by sinusoidal vessels and capillaries, with histological morphology similar to that of many species of fish. Melanomacrophage centers were found only in the most developed animals, but in small quantities, prompting the development of new research on this cell, in this species. In this way, research of this nature allows the characterization of fish species, helping to improve breeding methods, understanding pathological processes caused by diseases, and obtaining better productive capacity, serving an increasingly demanding and prosperous market.


Resumo O trabalho objetivou avaliar a relação peso-comprimento e o fator de condição, caracterizando a biometria, anatomia, histologia e densidade volumétrica do fígado de Brycon amazonicus, em diferentes estágios de crescimento corporal. O experimento utilizou vinte espécimes em quatro fases de crescimento corporal (PI, PII, PIII e PIV) colhidos a cada 90 dias, contendo cinco espécimes, cada grupo. Os fígados foram dissecados, pesados (g) e processados rotineiramente pela técnica da hematoxilina e eosina. Os dados foram submetidos à análise de variância, teste de correlação de Pearson e regressão linear. A equação que representou a relação peso-comprimento foi W = 0,05902 x L2,63, com crescimento alométrico negativo, mas com fator de condição relativo igual a 1,0. O fígado apresentou-se dividido em três lobos com a vesícula biliar próxima ao lobo direito e sua cor variou de vermelho claro a vermelho escuro, não variando em relação a outras espécies de peixes. A relação hepatossomática acompanhou o crescimento corporal até a fase do grupo PII e então declinou, demonstrando o comportamento de seu desenvolvimento em Brycon amazonicus. O órgão constitui-se predominantemente por hepatócitos, seguido de vasos e capilares sinusoidais, com a morfologia histológica semelhante ao de muitas espécies de peixes. Centros melanomacrófagos foram encontrados apenas nos animais mais desenvolvidos, mas em pequena quantidade, instigando o desenvolvimento de novas pesquisas sobre esta célula, nesta espécie. Desta forma, pesquisas desta natureza permitem a caracterização de espécies de peixes, auxiliando no aperfeiçoamento de métodos de criação, compreensão de processos patológicos provocados por enfermidades, e obtenção de melhor capacidade produtiva, atendendo um mercado cada vez mais exigente e próspero.

2.
Braz J Biol ; 84: e248656, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34730686

RESUMO

Several species of Cichla successfully colonized lakes and reservoirs of Brazil, since the 1960's, causing serious damage to local wildlife. In this study, 135 peacock bass were collected in a reservoir complex in order to identify if they represented a single dominant species or multiple ones, as several Cichla species have been reported in the basin. Specimens were identified by color pattern, morphometric and meristic data, and using mitochondrial markers COI, 16S rDNA and Control Region (CR). Overlapping morphological data and similar coloration patterns prevented their identification using the taxonomic keys to species identification available in the literature. However, Bayesian and maximum likelihood from sequencing data demonstrated the occurrence of a single species, Cichla kelberi. A single haplotype was observed for the 16S and CR, while three were detected for COI, with a dominant haplotype present in 98.5% of the samples. The extreme low diversity of the transplanted C. kelberi evidenced a limited number of founding maternal lineages. The success of this colonization seems to rely mainly on abiotic factors, such as increased water transparency of lentic environments that favor visual predators that along with the absence of predators, have made C. kelberi a successful invader of these reservoirs.


Assuntos
Ciclídeos , Animais , Teorema de Bayes , Ciclídeos/genética , Variação Genética/genética , Haplótipos/genética , Lagos , Filogenia
3.
Cien Saude Colet ; 26(suppl 3): 5013-5022, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34787194

RESUMO

Studies evaluating the factors associated with under reporting and with the recognition and reporting of child physical abuse are scarce and highly necessary. This study aimed to assess the prevalence of recognition and reporting of child physical abuse (CPA) by Brazilian dentistsin primary care and associated factors. A cross-sectional study was carried out with a representative sample of dentistsfrom the Family Health Strategy in Belo Horizonte. A self-administered questionnaire validated to Brazilian Portuguesewas used for data collection. Statistical analysis included univariate and multiple analyses through Poisson regression. A total of 181dentists participated in the study. Among them, 73 (40.3%) had already recognized some cases of CPA, but only 11 (6.1%) ended up reporting. Dentists with six to 19 years of experience as a municipal worker presented 2.38 times [PR = 2.38 (95%CI: 1.29-4.41)] more probability to recognize cases of CPA than the ones with less than six years. Having a graduate degree with a major in childcare [PR = 4.50 (95%CI: 1.08-18.68)] was associated with a larger number of reports. The employment duration as municipal worker was positively associated with the recognition of CPA cases and the prevalence of reporting was low.


Assuntos
Maus-Tratos Infantis , Cirurgiões , Criança , Estudos Transversais , Humanos , Abuso Físico , Prevalência , Inquéritos e Questionários
4.
PLoS Negl Trop Dis ; 15(9): e0009734, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-34555016

RESUMO

BACKGROUND: Treatment of cutaneous leishmaniasis (CL) remains challenging since the drugs currently used are quite toxic, thus contributing to lethality unrelated to the disease itself but to adverse events (AE). The main objective was to evaluate different treatment regimens with meglumine antimoniate (MA), in a reference center in Rio de Janeiro, Brazil. METHODOLOGY: A historical cohort of 592 patients that underwent physical and laboratory examination were enrolled between 2000 and 2017. The outcome measures of effectiveness were epithelialization and complete healing of cutaneous lesions. AE were graded using a standardized scale. Three groups were evaluated: Standard regimen (SR): intramuscular (IM) MA 10-20 mg Sb5+/kg/day during 20 days (n = 46); Alternative regimen (AR): IM MA 5 mg Sb5+/kg/day during 30 days (n = 456); Intralesional route (IL): MA infiltration in the lesion(s) through subcutaneous injections (n = 90). Statistical analysis was performed through Fisher exact and Pearson Chi-square tests, Kruskal-Wallis, Kaplan-Meier and log-rank tests. RESULTS: SR, AR and IL showed efficacy of 95.3%, 84.3% and 75.9%, with abandonment rate of 6.5%, 2.4% and 3.4%, respectively. IL patients had more comorbidities (58.9%; p = 0.001), were mostly over 50 years of age (55.6%), and had an evolution time longer than 2 months (65.6%; p = 0.02). Time for epithelialization and complete healing were similar in IL and IM MA groups (p = 0.9 and p = 0.5; respectively). Total AE and moderate to severe AE that frequently led to treatment interruption were more common in SR group, while AR and IL showed less toxicity. CONCLUSIONS/SIGNIFICANCE: AR and IL showed less toxicity and may be good options especially in CL cases with comorbidities, although SR treatment was more effective. IL treatment was an effective and safe strategy, and it may be used as first therapy option as well as a rescue scheme in patients initially treated with other drugs.

5.
Enferm. foco (Brasília) ; 12(2): 277-282, set. 2021. tab
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1291392

RESUMO

Objetivo: Descrever as representações sociais da Diabetes Mellitus para indivíduos diabéticos e para profissionais de saúde que assistem a estes pacientes. Metodologia: Estudo descritivo, exploratório, com enfoque qualitativo, à luz da Teoria das Representações Sociais de Serge Moscovici. Desenvolvido a partir de um documento semiestruturado dividido em perfil sócio demográfico e questões voltadas ao objeto de pesquisa através de evocação de palavras. A amostra foi composta por 14 pessoas, entre pacientes e profissionais de saúde presentes no Ambulatório de lesões de um hospital público, na cidade de Recife- PE. Resultados: O grupo de pacientes relacionou a doença à palavras de cunho emocional, como medo/tristeza, enquanto os profissionais evocaram palavras mais técnicas e voltadas ao controle e prevenção da patologia. Conclusões: O nível de conhecimento está diretamente ligado a representatividade da doença para o indivíduo, interferindo na adesão terapêutica e dificultando a aceitação da condição, implicando em mais agravos físicos ou psicológicos. (AU)


Objective: Describe the social representations of Diabetes Mellitus for diabetic individuals and for health professionals who assist these patients. Methods: Descriptive study, exploratory, with qualitative approach, in the light of the Theory of Social Representations by Serge Moscovici. Developed from a semi-structured document divided into demographic profile and issues facing the object of research through evocation of words. The sample was composed by 14 people, among patients and health professionals present in the Lesions Outpatient Clinic from a public hospital, in the city of Recife ­ PE. Results: The group of patients related to disease with words of emotional nature as fear/sadness, but the professionals have raised more technical words and aimed at the control and prevention of the disease. Conclusion: The level of knowledge is directly connected to the representativeness of the disease for the individual, interfering in the therapeutic adhesion and hindering the acceptance of the condition, resulting in more grievances physical or psychological. (AU)


Objetivo: Describa las representaciones sociales de la diabetes mellitus para las personas con diabetes y para los profesionales de la salud que ayudan a estos pacientes. Metodos: Estudio exploratorio descriptivo con enfoque cualitativo, a la luz de la Teoría de las representaciones sociales de Serge Moscovici. Desarrollado a partir de un documento semiestructurado dividido en un perfil sociodemográfico y preguntas centradas en el objeto de investigación a través de la evocación de palabras. La muestra consistió en 14 personas, incluidos pacientes y profesionales de la salud presentes en la Clínica de Lesiones de un hospital público, en la ciudad de Recife-PE. Resultados: El grupo de pacientes relacionó la enfermedad con palabras emocionales, como miedo / tristeza, mientras que los profesionales evocaron palabras más técnicas destinadas a controlar y prevenir la patología. Conclusión: El nivel de conocimiento está directamente relacionado con la representatividad de la enfermedad para el individuo, lo que interfiere con la adherencia terapéutica y dificulta la aceptación de la condición, lo que resulta en más problemas físicos o psicológicos. (AU)


Assuntos
Diabetes Mellitus , Percepção Social , Enfermagem , Pé Diabético
6.
Res Vet Sci ; 140: 56-63, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34399281

RESUMO

The proteoglycan versican (VCAN) plays a complex role in cancer. The expression of this molecule has been related to invasion and progression in malignant mixed tumors, such as carcinoma in mixed tumors (CMT) of the canine mammary gland. In addition, its interaction with surface cell receptors EGFR, HER-2 and CD44 in malignant epithelial cells may be responsible for proliferation and cellular motility in early stages of cancer. We comparatively evaluated the expression of this proteoglycan and its receptors in in situ and invasive areas of simple carcinomas (SC) and CMT to investigate similarities and differences between these histological types. Immunohistochemistry was performed with anti-VCAN, anti-CD44, anti-EGFR and anti-HER-2 antibodies in 32 cases of SC or CMT. VCAN was highly expressed in stroma adjacent to invasive areas in SC and CMT. CMTs presented comparatively higher expression of VCAN in stroma adjacent to in situ and in invasive areas than in corresponding areas in SCs. In CMT, EGFR and HER-2 expressions were higher in situ compared to invasive areas. In contrast, increased CD44 and EGFR expression was found in invasive areas in SC compared to CMT. These results indicate that versican expression is similarly associated with invasiveness in SC and CMT, however higher levels were seen in CMT suggesting that the presence of myoepithelial proliferation in this tumor type participates in stromal composition and promoting an increase in the expression of versican.


Assuntos
Carcinoma , Doenças do Cão , Neoplasias Mamárias Animais , Animais , Carcinoma/veterinária , Proliferação de Células , Cães , Imuno-Histoquímica , Versicanas/genética
7.
Dement Neuropsychol ; 15(2): 223-229, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34345364

RESUMO

The co-occurrence of post-stroke behavioral disorders and cognitive impairment has been extensively investigated. However, studies usually do not include social cognition among the assessed cognitive domains. Objective: To investigate the potential association between facial emotion recognition, a measure of social cognition, and behavioral and cognitive symptoms in the subacute phase of ischemic stroke. Methods: Patients admitted to a Stroke Unit with ischemic stroke were followed up to 60 days. At this time point, they were evaluated with the following tools: Mini-Mental State Examination (MMSE); Frontal Assessment Battery (FAB); Visual Memory Test of the Brief Cognitive Battery (VMT); Phonemic Verbal Fluency (F-A-S Test); Digit Span; Facial Emotion Recognition Test (FERT) and Hospital Anxiety and Depression Scale (HADS). A control group composed of 21 healthy individuals also underwent the same evaluation. Results: Eighteen patients with ischemic stroke were enrolled in this study. They had similar age, sex and schooling years compared to controls. Depression symptoms and episodic memory deficits were significantly more frequent in patients compared to controls. The recognition of sadness expression positively correlated with the levels of anxiety and depression, while and the recognition of fear expression negatively correlated with depression in the stroke group. Conclusions: After an ischemic stroke, patients exhibit impairment in social cognition skills, specifically facial emotion recognition, in association with behavioral symptoms.

8.
Res Vet Sci ; 139: 87-93, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34265511

RESUMO

Regional lymph node status impacts survival in dogs with malignant mammary tumors. However, few studies have evaluated extracapsular extension and tumor implants in regional lymph node metastases in dogs with mammary carcinoma. Therefore, 84 cases of mammary carcinomas with metastases in inguinal and/or axillary lymph nodes from female dogs of different breeds and a total of 139 metastatic lymph nodes were evaluated by routine histological staining. Clinical and pathological characteristics of primary tumors as well as the presence of extracapsular extension and tumor implants in the lymph nodes were analyzed, in addition to survival. One to 5 lymph nodes were evaluated in each case. Extracapsular extension and tumor implants were present in 17.9% and 39.3% of cases, respectively. The simultaneous presence of extracapsular extension and tumor implants were associated with an increased risk of death (hazard ratio 10.46). In addition, "special type carcinomas", high histological grade (grade III), and presence of extracapsular extension associated with tumor implants were related to a worse prognosis and shorter survival times (p < 0.05). Based on these results, we highlight the importance of identifying extracapsular extension and tumor implants in dogs with metastatic mammary carcinomas, as they are associated with a higher risk of death and shorter survival.


Assuntos
Carcinoma , Doenças do Cão , Metástase Linfática , Animais , Carcinoma/veterinária , Cães , Extensão Extranodal , Feminino , Linfonodos , Prognóstico , Análise de Sobrevida
9.
Braz J Biol ; 83: e244784, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34190759

RESUMO

The work aimed to evaluate the weight-length relationship and the condition factor, characterizing the biometry, anatomy, histology and volumetric density of the liver of Brycon amazonicus, in different stages of body growth. The experiment used twenty specimens in four stages of body growth (PI, PII, PIII and PIV) harvested every 90 days, containing five specimens, each group. The livers were dissected, weighed (g) and processed routinely using the hematoxylin and eosin technique. The data were submitted to analysis of variance, Pearson's correlation test and linear regression. The equation that represented the weight-length relation was W = 0.05902 x L2.63, with negative allometric growth, but with a relative condition factor equal to 1.0. The liver was divided into three lobes with the gallbladder close to the right lobe and its color varied from light red to dark red, not varying in relation to other fish species. The hepatosomatic relationship followed body growth until the PII group stage and then declined, demonstrating the behavior of its development in Brycon amazonicus. The organ consists predominantly of hepatocytes, followed by sinusoidal vessels and capillaries, with histological morphology similar to that of many species of fish. Melanomacrophage centers were found only in the most developed animals, but in small quantities, prompting the development of new research on this cell, in this species. In this way, research of this nature allows the characterization of fish species, helping to improve breeding methods, understanding pathological processes caused by diseases, and obtaining better productive capacity, serving an increasingly demanding and prosperous market.


Assuntos
Caraciformes , Animais , Fígado
10.
Enferm. foco (Brasília) ; 12(1): 196-201, jun. 2021. ilus
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1280852

RESUMO

Objetivo: Descrever o desenvolvimento de um protótipo de game educativo para prevenção de acidentes comuns na infância. Metodologia: Estudo descritivo sobre desenvolvimento tecnológico de um protótipo de game educativo, proveniente de pesquisa original sobre utilização da gamificação como ferramenta educativa para prevenção de acidentes na infância. O desenvolvimento do protótipo guiou-se pela estratégia Design Thinking, com uso do software educacional Scratch que é uma plataforma online, gratuita. Os cenários e personagens foram construídos com figuras disponíveis em acesso livre na internet. Resultados: O protótipo do game foi intitulado "Detetives do perigo", com público alvo crianças entre 8 a 10 anos de idade. As fases do game apresenta situações de risco de acidente na rotina diária da criança, e explica de forma simples e interativa como evitá-los. O game é composto por dez cenários, sendo quatro de orientações e seis dos ambientes domiciliar, escolar, via e parque público, onde são expostas as situações de risco de acidente. Conclusão: O uso de ferramentas tecnológicas nas ações de educação em saúde, permite que a criança seja protagonista no cuidado com sua saúde e bem-estar, o despertar do senso crítico compatível com sua faixa etária, pode contribuir na redução de acidentes na infância. (AU)


Objective: To describe the development of an educational game prototype for the prevention of common accidents in childhood. Methods: Descriptive study on technological development of an educational game prototype, from original research on the use of gamification as an educational tool for the prevention of childhood accidents. The development of the prototype was guided by the Design Thinking strategy, using scratch educational software which is a free online platform. The scenarios and characters were built with figures available in free internet access. Results: The prototype of the game was titled "Danger Detectives", with audience targeting children between 8 to 10 years of age. The phases of the game presents situations of risk of accident in the daily routine of the child and explains in a simple and interactive way how to avoid them. The game consists of ten scenarios, four of which are guidelines and six of the home, school, road and public park environments, where accident risk situations are exposed. Conclusion: The use of technological tools in health education actions allows the child to be a protagonist in the care of their health and well-being, the awakening of critical sense compatible with their age group can contribute to the reduction of accidents in childhood. (AU)


Objetivo: Describir el desarrollo de un prototipo de juego educativo para la prevención de accidentes comunes en la infância. Methods: Estudio descriptivo sobre desarrollo tecnológico de un prototipo de juego educativo, derivado de la investigación original sobre el uso de la gamificación como herramienta educativa para la prevención de accidentes infantiles. El desarrollo del prototipo fue guiado por la estrategia Design Thinking, utilizando software educativo scratch que es una plataforma en línea gratuita. Los escenarios y personajes fueron construidos con figuras disponibles en acceso gratuito a Internet. Resultados: El prototipo del juego se tituló "Detectives de peligro", con un público dirigido a niños de entre 8 y 10 años de edad. Las fases del juego presentan situaciones de riesgo de accidente en la rutina diaria del niño, y explican de una manera sencilla e interactiva cómo evitarlos. El juego consta de diez escenarios, cuatro de los cuales son pautas y seis de los ambientes de hogar, escuela, carretera y parque público, donde las situaciones de riesgo de accidentes están expuestas. Conclusión: El uso de herramientas tecnológicas en las acciones de educación sanitaria permite al niño ser protagonista en el cuidado de su salud y bienestar, el despertar del sentido crítico compatible con su grupo de edad puede contribuir a la reducción de los accidentes en la infancia. (AU)


Assuntos
Prevenção de Acidentes , Jogos e Brinquedos , Saúde da Criança , Educação em Saúde , Informática em Enfermagem
11.
Braz. J. Biol. ; 81(2): 251-257, Mar.-May 2021. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-762748

RESUMO

Genetically modified plants are one of the tactics used in integrated pest management - IPM. There is great concern about the impact of these plants on non-target organisms. On the other hand, there is little information in the literature on the effects of transgenics (Bacillus thuringiensis) Bt on populations of phytophagous mites, and the physiological responses that this attack promotes on plants. The objective of this work was to evaluate the biology of the T. ludeni mite in Bt cotton, expressing the Cry1F and Cry1Ac proteins. To evaluate the behavior of food and oviposition preference of the T. ludeni with Bt cotton and isohybrid. Verify if the physiological stress caused by T. ludenis attack is differentiated in Bt cotton. The mites were reared in Bt cotton and isohybrid, in a total of 40 replicates in the completely randomized design and the biological cycle was evaluated. The food preference and oviposition analysis were done with 10 replicates, with choice. The physiological stress was evaluated through chlorophyll fluorescence, under greenhouse conditions. The data of the T. ludeni biology were analyzed by Student's t-test, for food and oviposition preference the chi-square test was performed. Regression models were fitted for the fluorescence parameters. The model identity test was used to evaluate the differences between Bt and isohybrid treatments. Cry1F and Cry1Ac proteins have not affected the biology of T. ludeni. The photosynthetic parameters in Bt cotton plants were less influenced by T. ludeni infestation.(AU))


O uso de plantas geneticamente modificadas é uma das táticas utilizadas no manejo integrado de pragas - MIP. Observa-se grande preocupação com o impacto dessas plantas sobre organismos não alvos. Por outro lado, existe pouca informação na literatura sobre efeitos dos transgênicos (Bacillus thuringiensis) Bt em populações de ácaros fitófagos, e as respostas fisiológicas que esse ataque promove nas plantas. Objetivou-se com esse trabalho avaliar a biologia do ácaro T. ludeni em algodoeiro Bt, expressando as proteínas Cry1F e Cry1Ac. Avaliar se há comportamento de preferência alimentar e postura de T. ludeni em relação ao algodoeiro Bt e seu iso-híbrido. E verificar se o estresse fisiológico causado pelo ataque de T. ludeni é diferenciado em algodoeiro Bt. Os ácaros foram criados em algodoeiro Bt e iso-híbrido, em um total de 40 repetições no delineamento inteiramente casualizado, onde foi avaliado o ciclo biológico. A análise de preferência alimentar e de posturas foi feita com 10 repetições, com escolha. O estresse fisiológico foi avaliando através da fluorescência da clorofila, em casa de vegetação. Os dados da biologia de T. ludeni foram analisados pelo teste t Student, para preferência alimentar e postura foi realizado o teste qui-quadrado. Para os parâmetros da fluorescência, foram ajustados modelos de regressão. Para testar as diferenças entre Bt e iso-híbrido foi utilizado o teste de identidade de modelos. As proteínas Cry1F e Cry1Ac não afetaram a biologia de T. ludeni. Os parâmetros fotossintéticos em plantas de algodoeiro Bt foram menos influenciados pela infestação de T. ludeni.(AU)


Assuntos
Tetranychidae/crescimento & desenvolvimento , Estresse Fisiológico , Gossypium/parasitologia , Controle de Pragas , Plantas Geneticamente Modificadas
12.
Braz. j. biol ; 81(2): 251-257, Jan.-May 2021. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1153347

RESUMO

Abstract Genetically modified plants are one of the tactics used in integrated pest management - IPM. There is great concern about the impact of these plants on non-target organisms. On the other hand, there is little information in the literature on the effects of transgenics (Bacillus thuringiensis) Bt on populations of phytophagous mites, and the physiological responses that this attack promotes on plants. The objective of this work was to evaluate the biology of the T. ludeni mite in Bt cotton, expressing the Cry1F and Cry1Ac proteins. To evaluate the behavior of food and oviposition preference of the T. ludeni with Bt cotton and isohybrid. Verify if the physiological stress caused by T. ludeni's attack is differentiated in Bt cotton. The mites were reared in Bt cotton and isohybrid, in a total of 40 replicates in the completely randomized design and the biological cycle was evaluated. The food preference and oviposition analysis were done with 10 replicates, with choice. The physiological stress was evaluated through chlorophyll fluorescence, under greenhouse conditions. The data of the T. ludeni biology were analyzed by Student's t-test, for food and oviposition preference the chi-square test was performed. Regression models were fitted for the fluorescence parameters. The model identity test was used to evaluate the differences between Bt and isohybrid treatments. Cry1F and Cry1Ac proteins have not affected the biology of T. ludeni. The photosynthetic parameters in Bt cotton plants were less influenced by T. ludeni infestation.


Resumo O uso de plantas geneticamente modificadas é uma das táticas utilizadas no manejo integrado de pragas - MIP. Observa-se grande preocupação com o impacto dessas plantas sobre organismos não alvos. Por outro lado, existe pouca informação na literatura sobre efeitos dos transgênicos (Bacillus thuringiensis) Bt em populações de ácaros fitófagos, e as respostas fisiológicas que esse ataque promove nas plantas. Objetivou-se com esse trabalho avaliar a biologia do ácaro T. ludeni em algodoeiro Bt, expressando as proteínas Cry1F e Cry1Ac. Avaliar se há comportamento de preferência alimentar e postura de T. ludeni em relação ao algodoeiro Bt e seu iso-híbrido. E verificar se o estresse fisiológico causado pelo ataque de T. ludeni é diferenciado em algodoeiro Bt. Os ácaros foram criados em algodoeiro Bt e iso-híbrido, em um total de 40 repetições no delineamento inteiramente casualizado, onde foi avaliado o ciclo biológico. A análise de preferência alimentar e de posturas foi feita com 10 repetições, com escolha. O estresse fisiológico foi avaliando através da fluorescência da clorofila, em casa de vegetação. Os dados da biologia de T. ludeni foram analisados pelo teste t Student, para preferência alimentar e postura foi realizado o teste qui-quadrado. Para os parâmetros da fluorescência, foram ajustados modelos de regressão. Para testar as diferenças entre Bt e iso-híbrido foi utilizado o teste de identidade de modelos. As proteínas Cry1F e Cry1Ac não afetaram a biologia de T. ludeni. Os parâmetros fotossintéticos em plantas de algodoeiro Bt foram menos influenciados pela infestação de T. ludeni.

13.
Toxicon ; 198: 171-175, 2021 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-34029603

RESUMO

Photobiomodulation using light-emitting diode (LED) treatment has analgesic and anti-inflammatory effects which can be an effective therapeutic associated with serum therapy for local treatment of snakebites. Here we explored the effects of LED treatment on isolated macrophage under Bothrops jararacussu venom. Results showed that LED induced IL-6 and TNF-α genes down-regulation and, TGF and ARG1 genes up-regulation which indicates a polarization of macrophages to an M2 phenotype contributing to both tissue repair and resolution of inflammation.


Assuntos
Bothrops , Venenos de Crotalídeos , Terapia com Luz de Baixa Intensidade , Animais , Macrófagos , Camundongos , Fenótipo
14.
J Laryngol Otol ; 135(5): 420-425, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33952362

RESUMO

OBJECTIVE: Tympanostomy is one of the most commonly performed surgical procedures in otolaryngology, and its complexity is challenging for trainee surgeons. Investing in medical education is a cornerstone of good patient safety practices. For trainees, use of simulators before operating on actual patients helps mitigate risks. This study aimed to develop a three-dimensional printed model simulator for myringotomy, tympanostomy and ventilation tube placement. METHODS: An articulated model with a detachable portion, base and plastic bag to simulate the external auditory canal, middle ear and tympanic membrane, respectively, was modelled and printed. RESULTS: The final simulator was made from acrylonitrile butadiene styrene polymer and measured 4 × 4 × 12 cm. It was designed to mimic the angulation of patient anatomy in the myringotomy position and simulate the texture and colour of the tissues of interest. The cost was low, and testing with an operating microscope and endoscope yielded satisfactory results. The advent of three-dimensional printing technology has made surgical simulation more accessible and less expensive, providing several advantages for medical education. CONCLUSION: The proposed model fulfilled expectations as a safe, inexpensive, reproducible, user-friendly and accessible surgical education tool that can be improved and reassessed for further research.


Assuntos
Endoscopia/educação , Microcirurgia/educação , Ventilação da Orelha Média/educação , Modelos Anatômicos , Impressão Tridimensional , Treinamento por Simulação , Adulto , Criança , Pré-Escolar , Competência Clínica , Humanos , Internato e Residência
15.
Dement. neuropsychol ; 15(2): 223-229, Apr.-June 2021. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1286199

RESUMO

ABSTRACT. The co-occurrence of post-stroke behavioral disorders and cognitive impairment has been extensively investigated. However, studies usually do not include social cognition among the assessed cognitive domains. Objective: To investigate the potential association between facial emotion recognition, a measure of social cognition, and behavioral and cognitive symptoms in the subacute phase of ischemic stroke. Methods: Patients admitted to a Stroke Unit with ischemic stroke were followed up to 60 days. At this time point, they were evaluated with the following tools: Mini-Mental State Examination (MMSE); Frontal Assessment Battery (FAB); Visual Memory Test of the Brief Cognitive Battery (VMT); Phonemic Verbal Fluency (F-A-S Test); Digit Span; Facial Emotion Recognition Test (FERT) and Hospital Anxiety and Depression Scale (HADS). A control group composed of 21 healthy individuals also underwent the same evaluation. Results: Eighteen patients with ischemic stroke were enrolled in this study. They had similar age, sex and schooling years compared to controls. Depression symptoms and episodic memory deficits were significantly more frequent in patients compared to controls. The recognition of sadness expression positively correlated with the levels of anxiety and depression, while and the recognition of fear expression negatively correlated with depression in the stroke group. Conclusions: After an ischemic stroke, patients exhibit impairment in social cognition skills, specifically facial emotion recognition, in association with behavioral symptoms.


RESUMO. A co-ocorrência de distúrbios comportamentais e comprometimento cognitivo pós-acidente vascular cerebral (AVC) é amplamente descrita na literatura. No entanto, os estudos geralmente não incluem a cognição social entre os domínios cognitivos avaliados. Objetivo: Investigar a potencial associação entre o reconhecimento da emoção facial, uma medida da cognição social, e os sintomas comportamentais e cognitivos na fase subaguda do AVC isquêmico. Métodos: Pacientes internados em uma Unidade de AVC com AVC isquêmico foram acompanhados até 60 dias, quando foram avaliados com os seguintes instrumentos: Mini-Exame do Estado Mental (MEEM); Bateria de Avaliação Frontal (FAB); Teste de Memória Visual da Bateria Cognitiva Breve (VMT); Fluência Verbal Fonêmica (Teste F-A-S); Span de dígitos; Teste de Reconhecimento de Emoção Facial (FERT) e Escala Hospitalar de Ansiedade e Depressão (HADS). Um grupo controle constituído por 21 indivíduos saudáveis também foi submetido à mesma avaliação. Resultados: Dezoito pacientes com AVC isquêmico foram incluídos no presente estudo, apresentando idade, sexo e anos de escolaridade semelhantes aos do grupo controle. Os sintomas de depressão e déficits de memória episódica foram significativamente mais frequentes em pacientes com AVC. O reconhecimento da expressão de tristeza correlacionou-se positivamente com os níveis de ansiedade e depressão, ao passo que o reconhecimento da expressão de medo correlacionou-se negativamente com depressão no grupo de AVC. Conclusões: Após um AVC isquêmico, pacientes podem apresentar alterações de cognição social, especificamente de reconhecimento da emoção facial, em associação com sintomas comportamentais.


Assuntos
Humanos , Cognição , Manifestações Neurocomportamentais , Acidente Vascular Cerebral , Depressão , Disfunção Cognitiva
16.
Polymers (Basel) ; 13(8)2021 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-33924112

RESUMO

Poly (methyl methacrylate) (PMMA) is a thermoplastic synthetic polymer, which displays superior characteristics such as transparency, good tensile strength, and processability. Its performance can be improved by surface engineering via the use of functionalized thin film coatings, resulting in its versatility across a host of applications including, energy harvesting, dielectric layers and water purification. Modification of the PMMA surface can be achieved by atomic layer deposition (ALD), a vapor-phase, chemical deposition technique, which permits atomic-level control. However, PMMA presents a challenge for ALD due to its lack of active surface sites, necessary for gas precursor reaction, nucleation, and subsequent growth. The purpose of this review is to discuss the research related to the employment of PMMA as either a substrate, support, or masking layer over a range of ALD thin film growth techniques, namely, thermal, plasma-enhanced, and area-selective atomic layer deposition. It also highlights applications in the selected fields of flexible electronics, biomaterials, sensing, and photocatalysis, and underscores relevant characterization techniques. Further, it concludes with a prospective view of the role of ALD in PMMA processing.

17.
Clin Transl Oncol ; 23(9): 1955-1960, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-33840046

RESUMO

INTRODUCTION: The aim of this study was to analyze the associations between perceived social support and sociodemographic variables on coping strategies. METHODS: A prospective, cross-sectional, multicenter study was conducted in 404 women with resected, non-metastatic breast cancer. Participants completed questionnaires: perceived social support (MSPSS), coping strategies (Mini-MAC), and psychological distress (BSI-18). RESULTS: Sociodemographic factors as age, education, and partnership status were associated with coping strategies. As for maladaptive strategies, hopelessness was more frequent in older people and lower educational level; fatalism in older and single people, and cognitive avoidance was associated with lower educational level. Suppor t from family, friends, and partners was associated with a greater fighting spirit. In contrast, high psychological distress (anxiety and depression) was associated with greater use of maladaptive strategies. CONCLUSION: Young people, a high level of education, having a partner, low psychological distress, and seeking social support were associated with the use of adaptive cancer coping strategies.

18.
Rev. SOBECC ; 26(1): 21-26, 31-03-2021.
Artigo em Inglês | LILACS | ID: biblio-1177427

RESUMO

Objetivo: Identificar a percepção de enfermeiros do bloco operatório sobre os motivos para o cancelamento de cirurgias eletivas e as estratégias para reduzir as taxas de suspensão. Método: Estudo de abordagem qualitativa realizado em um hospital de alta complexidade do Recife, Pernambuco, Brasil, com enfermeiros atuantes no bloco operatório. A coleta de dados foi realizada por meio de entrevistas gravadas com sete enfermeiros responsáveis pelas cirurgias eletivas. Para o tratamento dos dados, utilizou-se a técnica da análise de conteúdo de Bardin. Resultados: Os discursos foram agrupados em duas categorias temáticas: lacunas no planejamento cirúrgico, com três subcategorias, e prevenção de situações evitáveis, com duas subcategorias. Conclusão: Os motivos para o cancelamento de cirurgias estavam relacionados ao planejamento do ambiente cirúrgico e à preparação clínica do paciente. Os enfermeiros identificaram esses motivos como situações evitáveis e consideraram que a comunicação efetiva e atitudes de liderança assertivas são estratégias que reduzem os índices de suspensão cirúrgica.


Objective: To identify the perception of surgical block nurses of the reasons for the cancellation of elective surgeries and strategies to reduce suspension rates. Method: This is a qualitative study carried out in a tertiary hospital of Recife, Pernambuco, Brazil, with nurses working in the surgical block. Data were collected through recorded interviews with seven nurses responsible for elective surgeries. Data treatment was based on Bardin's content analysis technique. Results: The statements were grouped into two thematic categories: gaps in surgical planning, with three subcategories, and prevention of avoidable situations, with two subcategories. Conclusion: The causes for surgery cancellation were related to surgical environment planning and the patient's clinical preparation. The nurses defined these reasons as avoidable situations and considered effective communication and assertive leadership attitudes as strategies that reduce surgical suspension rates.


Objetivo: Identificar la percepción de los enfermeros en el quirófano sobre los motivos de cancelación de las cirugías electivas y las estrategias para reducir las tasas de suspensión. Método: Estudio cualitativo, realizado en un hospital de alta complejidad en Recife, Pernambuco, Brasil, con enfermeros trabajando en quirófano. La recolección de datos se realizó mediante entrevistas grabadas con siete enfermeros responsables de cirugías electivas. Para el tratamiento de los datos se utilizó la técnica de Análisis de Contenido de Bardin (Bardin Content Analysis). Resultados: Los discursos se agruparon en dos categorías temáticas: brechas en la planificación quirúrgica, con tres subcategorías, y prevención de situaciones evitables, con dos subcategorías. Conclusión: Los motivos para cancelar cirugías estaban relacionados con la planificación del entorno quirúrgico y la preparación clínica del paciente. Los enfermeros identificaron estos motivos como situaciones prevenibles y consideraron que la comunicación eficaz y las actitudes de liderazgo asertivo son estrategias que reducen las tasas de suspensión quirúrgica.


Assuntos
Humanos , Salas Cirúrgicas , Cirurgia Geral , Enfermeiras e Enfermeiros , Prevenção de Doenças , Planejamento , Hospitais
19.
Nat Commun ; 12(1): 1244, 2021 02 23.
Artigo em Inglês | MEDLINE | ID: mdl-33623024

RESUMO

Differentiation between distinct stages is fundamental for the life cycle of intracellular protozoan parasites and for transmission between hosts, requiring stringent spatial and temporal regulation. Here, we apply kinome-wide gene deletion and gene tagging in Leishmania mexicana promastigotes to define protein kinases with life cycle transition roles. Whilst 162 are dispensable, 44 protein kinase genes are refractory to deletion in promastigotes and are likely core genes required for parasite replication. Phenotyping of pooled gene deletion mutants using bar-seq and projection pursuit clustering reveal functional phenotypic groups of protein kinases involved in differentiation from metacyclic promastigote to amastigote, growth and survival in macrophages and mice, colonisation of the sand fly and motility. This unbiased interrogation of protein kinase function in Leishmania allows targeted investigation of organelle-associated signalling pathways required for successful intracellular parasitism.


Assuntos
Diferenciação Celular , Leishmania mexicana/citologia , Leishmania mexicana/enzimologia , Animais , Proteína 9 Associada à CRISPR/metabolismo , Sistemas CRISPR-Cas/genética , Sobrevivência Celular , Feminino , Flagelos/enzimologia , Deleção de Genes , Leishmaniose/parasitologia , Leishmaniose/patologia , Camundongos Endogâmicos BALB C , Modelos Biológicos , Mutação/genética , Proteínas Quinases/genética , Proteínas Quinases/metabolismo , Proteoma/metabolismo , Psychodidae/parasitologia
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