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J Morphol ; 284(5): e21584, 2023 05.
Artigo em Inglês | MEDLINE | ID: mdl-36976835


This work reports on the structural characteristics of the respiratory gas bladder of the osteoglossiform fish Heterotis niloticus. The bladder-vertebrae relationships are also analyzed. A slit-shaped orifice in the mediodorsal pharyngeal wall is surrounded by a muscle sphincter and serves as a glottis-like opening to the gas bladder. The dorsolateral internal surface of the gas bladder is lined by a parenchyma of highly vascularized trabeculae and septa displaying an alveolar-like structure. The trabeculae contain, in addition to vessels, numerous eosinophils probably involved in immune responses. The air spaces are endowed with a thin exchange barrier indicating a good potential for respiratory gas exchange. The ventral wall of the gas bladder is a well-vascularized membrane that exhibits an exchange barrier in the luminal face and an inner structure dominated by the presence of a layer of richly innervated smooth muscle. This is suggestive of an autonomous adjustability of the gas bladder ventral wall. The trunk vertebrae show large transverse processes (parapophyses) and numerous surface openings that lead into intravertebral spaces that become invaded by the bladder parenchyma. Curiously, the caudal vertebrae show a regular teleost morphology with neural and hemal arches, but have similar surface openings and intravertebral pneumatic spaces. The African Arowana hence rivals the freshwater butterfly fish Pantodon in its exceptional role of displaying postcranial skeletal pneumaticity outside of Archosauria. The possible significance of these findings is discussed.

Coluna Vertebral , Bexiga Urinária , Animais , Coluna Vertebral/anatomia & histologia , Peixes/anatomia & histologia , Osso Esponjoso , Faringe
J Fish Biol ; 96(2): 533-538, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31808545


The taxonomic, morphological and meristic features of a dark eel caught by commercial trawling off the Gulf of Patti, southern Tyrrhenian Sea, Mediterranean Sea, February 17, 2017, showed it to be a female Conger conger. Histological analysis of gonads demonstrated that the individual was in a post-spawning phase. X-ray radiographs showed widespread decalcification of skeleton and teeth loss, confirming a strong mobilisation of somatic energy reserves needed for reproductive development.

Enguias/fisiologia , Animais , Calcificação Fisiológica , Feminino , Mar Mediterrâneo , Reprodução/fisiologia
J Morphol ; 276(1): 90-101, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25130402


We report here on the macroscopic, light microscopic, and electron microscopic structure of the gas bladder (GB) of the spotted gar, Lepisosteus oculatus. The GB opens into the pharynx, dorsal to the opening of the oesophagus, through a longitudinal slit bordered by two glottal ridges. Caudal to the ridges, the GB is an elongated sac divided into a central duct and right and left lobes. The lobes are formed by a cranio-caudal sequence of large air spaces that open into the central duct. The structure of the GB is that of a membranous sac supported by a system of septa arising from the walls of a central duct. The septa contain variable amounts of striated and smooth muscle might function to maintain the bladder shape and in providing contractile capabilities. The presence of muscle cells, nerves, and neuroepithelial cells in the wall of the GB strongly suggests that GB function is tightly regulated. The central duct and the apical surface of the thickest septa are covered by mucociliated epithelium. Most of the rest of the inner bladder surface is covered by a respiratory epithelium which contains goblet cells and a single type of pneumocyte. These two cell types produce surfactant. The respiratory barrier contains thick areas with fibrillar material and cell prolongations, and thin areas that only contain basement membrane material between the capillary wall and the respiratory epithelium. Lungs and GBs share many anatomical and histological features. There appears to be no clear criterion for structural distinction between these two types of respiratory organs.

Peixes/anatomia & histologia , Sistema Respiratório/anatomia & histologia , Animais , Peixes/fisiologia
Ann Anat ; 196(4): 178-82, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24675055


The fish sensory epithelium contains three types of sensory cells denominated ciliated, microvillous, and crypt neurons. Each one differs from the other in its morphological, ultrastructural and molecular features, as well as in their projections to the central nervous system. Crypt neurons are present in both bony and cartilaginous fish and can be identified on the basis of their morphology and the expression of some specific proteins and genes. In this study we have investigated the morphology of crypt neurons, as well as the occurrence and co-localization of S100 protein, calretinin and TRPV4, three proposed markers for crypt cells, in the olfactory epithelium of adult zebrafish (Danio rerio) using double immunofluorescence associated to laser confocal microscopy. A sparse population of superficial S100 protein positive cells was detected being identified as crypt neurons. The calretinin immunoreactive cells were more abundant, occasionally resembling the morphology of the crypt cells but never displaying co-localization of both proteins. The TRPV4 positive cells differed in morphology from crypt cells, thus excluding the occurrence of TRPV4 in those cells. These results demonstrate that only S100 protein immunoreactivity can be used to identify crypt cells. Because some calretinin positive cells showed localization and morphology similar to the crypt cells of the sensory epithelium, the occurrence of two subtypes of crypt cells, S100 protein and calretinin positive, cannot be excluded. The significance of these findings remains to be elucidated.

Neurônios/fisiologia , Mucosa Olfatória/inervação , Peixe-Zebra/fisiologia , Animais , Calbindina 2/metabolismo , Imuno-Histoquímica , Proteínas S100/metabolismo , Células Receptoras Sensoriais/fisiologia , Olfato/fisiologia , Canais de Cátion TRPV/metabolismo , Proteínas de Peixe-Zebra/metabolismo
Microsc Res Tech ; 75(10): 1321-8, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22622998


The enigmatic rodlet cells (RCs) are characterized by conspicuous inclusions named "rodlets". They were discovered over 100 years ago and were considered as parasites but shortly afterward interpreted as endogenous cells. The RCs have been described in different tissues of marine and freshwater teleosts, but their origin and function remain unknown. This work was designed to an ultrastructural study on RCs development and distribution in intestinal epithelium of Dicentrarchus labrax. Three different stages of RCs development from early precursor cells to mature phase were observed, as well as a migration and finally an extrusion of their contents. In this study, the immature cells were found near the basal epithelium membrane. They were mainly identified by a rough endoplasmic reticulum with dilated cisternae, by developing rodlets and a thin fibrillar coat. The maturing RCs, localized in the middle zone of the epithelium, appeared to be undergoing a reorganization of the cell organelles. The mature RCs, placed near the free surface, showed a thick subplasmalemmar fibrillar coat. Most of the organelles were aggregated at the cell apex with a basally located nucleus. A cellular polarity was more evident. One of the most conspicuous features was the occurrence of mature rodlets club-sac in shape orientated toward the cell apex. Adhesive junctions between surface epithelial cells and RCs, while discharging their contents, were seen. We have connected morphological figures and distribution to different stages of development in RCs, supporting the hypothesis of their secretory function.

Bass , Mucosa Intestinal/citologia , Intestinos/citologia , Animais , Adesão Celular , Movimento Celular , Organelas/ultraestrutura