[Development of a technique for the determination of inulin in natural instant chicory after enzymatic hydrolysis by high-performance liquid chromatography].

A highly specific technique for estimating inulin content in natural instant chicory based on the determination of fructose after enzymatic hydrolysis with amyloglucosidase and inulinase by high-performance liquid chromatography with refractometric detection has been developed. Determination of inulin content was carried out by a system for high performance liquid chromatography Agilent 1260 Series with a refractometer, Waters Sugar-Pak column of 10 µm × 6.5 mm × 300 mm: isocratic elution mode (mobile phase - water) with a flow rate of 0.5 ml/min, column temperature - 80 °C, injected sample volume - 10 µl. Inulin content in samples of soluble natural chicory ranges from 4 to 25%. It has been shown that the developed technique can be applied in the quality control of products used for the preparation of soluble natural chicory beverages.

[Selection of the optimal method of extraction and determination of dye Sudan I-IV and Para Red by high-performance liquid chromatography].

Since 2004 was important the problem of determination of non-food dyes as Sudan I-IV and Para Red. Therefore it was necessary to develop reliable methodologies or adapt existing analytical methods for monitoring the presence of such dyes in food. For this purpose, we have previously proposed a method for high-performance liquid chromatography with diode-array and mass detection. However this method showed the urgent need to clarify this important phase of the study as the sample preparation. In the present work was studied the possibility of solving this problem such solvents as ethanol, chloroform, acetone, conducted the selection of the optimum ratio of extraction agent-raw. The authors have also refined the metrological characteristics of the method.

[Determination of dyes sudans in of raw foods].

The number of cases using of forbidden dyes (such as Sudan-I and Para Red) in European foodstuff (including foodstuff from Russia) were increased last time. In this case it was required to adapt existing analytical methods for the control of dyes Sudan I-IV and Para Red. It was used for this purpose high performance liquid chromatography with detectings on diode-array and MS detector. Sudan I and Para Red were detected in 3 foodstuffs from examined 49 products. Concentration levels of these dyes were varied from 3 up to 500 mg/kg. Limits of determination of the methods is: HPLC-diode-array--0.1 mg/kg and HPLC-MS--0.5 mg/kg.