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1.
Braz. j. biol ; 81(4): 940-953, Oct.-Dec. 2021. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1153447

RESUMO

Abstract Nowadays food borne illness is most common in people due to their epidemic nature. These diseases affect the human digestive system through bacteria, viruses and parasites. The agents of illness are transmitted in our body through various types of food items, water and uncooked. Pathogens show drastic changes in immunosuppressant people. This review gives general insights to harmful microbial life. Pakistan is a developed country and because of its improper food management, a lot of gastrointestinal problems are noted in many patients. Bacteria are most common agents to spread diarrhoea, villi infection, constipation and dysenteric disease in human and induce the rejection of organ transplant. Enhancement of their lifestyle, properly cooked food should be used and to overcome the outbreak of the diseases.


Resumo Hoje em dia, as doenças transmitidas por alimentos são mais comuns em pessoas devido à sua natureza epidêmica. Essas doenças afetam o sistema digestivo humano por meio de bactérias, vírus e parasitas. Os agentes das doenças são transmitidos em nosso corpo por meio de diversos tipos de alimentos, água e crus. Os patógenos mostram mudanças drásticas em pessoas imunossupressoras. Esta revisão fornece uma visão geral da vida microbiana prejudicial. O Paquistão é um país desenvolvido e, devido ao seu manejo alimentar inadequado, muitos problemas gastrointestinais são observados em muitos pacientes. As bactérias são os agentes mais comuns para espalhar diarreia, infecção de vilosidades, obstipação e doença disentérica em humanos e induzem a rejeição de transplantes de órgãos. Melhoria de seu estilo de vida, alimentos devidamente cozidos devem ser utilizados e para superar o aparecimento de doenças.

3.
AJR Am J Roentgenol ; : 1-6, 2021 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-34319163

RESUMO

BACKGROUND. Extrinsic warming of iodinated CT contrast media to body temperature reduces viscosity and injection pressures. However, studies examining the effect of extrinsic warming on clinical adverse events are limited in number and provide conflicting results. Therefore, consensus practice recommendations have been sparse. OBJECTIVE. The purpose of this study is to compare rates of extravasation, allergic and allergiclike reactions, and physiologic reactions between iohexol 350 mg I/mL warmed to body temperature (37°C) versus this agent maintained at room temperature. METHODS. This retrospective study compared adult patients who received CT examinations using IV iohexol 350 that had either been warmed to body temperature or maintained at room temperature. At our institution, contrast media had historically been warmed to body temperature before a protocol change unrelated to this investigation. Information on the patient and CT examination was extracted from the electronic medical record. Adverse events, including extravasations, allergic and allergiclike reactions, and physiologic reactions, were compared between groups. RESULTS. A total of 3939 patients received contrast media warmed to body temperature before the protocol change; 3933 patients received contrast media at room temperature after the protocol change. The body temperature group experienced 11 (0.28%; 95% CI, 0.14-0.50%) adverse events, all extravasations; the allergic and allergic-like reaction rate was 0.00% (97.5% CI, 0.00-0.09%). The room temperature group experienced 17 (0.43%; 95% CI, 0.25-0.69%) adverse events: 13 (0.33%; 95% CI, 0.17-0.56%) extravasations and four (0.10%; 95% CI, 0.03-0.26%) allergic and allergiclike reactions. No physiologic reaction occurred in either group. The two groups were not different in terms of overall reaction rate (p = .19), extravasation rate (p = .69), allergic and allergiclike reaction rate (p = .06), or physiologic reaction rate (p > .99). Logistic regression adjusting for patient and CT characteristics (age, sex, conventional CT vs CTA, contrast media volume, injection location) showed no significant association of patient group and adverse reaction rate (odds ratio, 2.19; 95% CI, 0.68-7.00). Multivariable regression modeling showed an excess of 0.27 adverse events per 100 patients within the room temperature group, which is below a 0.6% noninferiority margin. CONCLUSION. The data suggest that maintaining iohexol 350 at room temperature is noninferior to warming the agent to body temperature before injection. CLINICAL IMPACT. The resources involved to prewarm iohexol 350 before injection may not be warranted.

4.
Mymensingh Med J ; 30(3): 584-588, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34226441

RESUMO

The aim of the study was to assess the status of body mass index (BMI) and blood pressure in elderly male in comparison to younger male and to study the association of BMI and blood pressure with age. This analytical type of cross-sectional study was carried out in the Department of Physiology, Mymensingh Medical College, Mymensingh, Bangladesh from January 2019 to December 2019. A total number of 140 male subjects were included in this study. Among them seventy (70) younger males were taken as control group (Group I) and seventy (70) elderly males were taken as study group (Group II). Body mass index (BMI) was calculated as the weight in kilograms divided by the square of the height in meters (kg/m²). Systolic and diastolic blood pressure was measured by aneroid sphygmomanometer (ALPK2, Japan). Data were expressed as Mean±SD and statistical significance of difference among the group was calculated by unpaired student's 't' test. Pearson's correlation coefficient test was done to find the correlation of BMI, systolic blood pressure (SBP) & diastolic blood pressure (DBP) with age. In this study we found that mean BMI, systolic blood pressure & diastolic blood pressure were significantly higher in elderly male persons in comparison to younger male. Although the magnitude of correlation differed, we found that BMI, systolic blood pressure & diastolic blood pressure were positively correlated with age of the subjects. Based on the study carried out it can be concluded that due to aging process geriatric populations are more prone to development of hypertension and other cardiovascular complications than younger individuals.


Assuntos
Hipertensão , Idoso , Bangladesh/epidemiologia , Pressão Sanguínea , Índice de Massa Corporal , Estudos Transversais , Humanos , Hipertensão/epidemiologia , Masculino
5.
J Evid Based Dent Pract ; 21(1): 101495, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34051951

RESUMO

ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: A systematic review and meta-analysis of randomized clinical trials comparing rotary canal instrumentation techniques with manual instrumentation techniques in primary teeth. Manchanda S, Sardana D, Yiu CKY. Int Endod J. 2020;53(3):333-353. SOURCE OF FUNDING: Information not available. TYPE OF STUDY/DESIGN: Systematic review with meta-analysis of data.


Assuntos
Preparo de Canal Radicular , Dente Decíduo , Assistência Odontológica , Humanos
6.
J Cell Biol ; 220(6)2021 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-33914026

RESUMO

Rac1 GTPase is hyperactivated in tumors and contributes to malignancy. Rac1 disruption of junctions requires its effector PAK1, but the precise mechanisms are unknown. Here, we show that E-cadherin is internalized via micropinocytosis in a PAK1-dependent manner without catenin dissociation and degradation. In addition to internalization, PAK1 regulates E-cadherin transport by fine-tuning Rab small GTPase function. PAK1 phosphorylates a core Rab regulator, RabGDIß, but not RabGDIα. Phosphorylated RabGDIß preferentially associates with Rab5 and Rab11, which is predicted to promote Rab retrieval from membranes. Consistent with this hypothesis, Rab11 is activated by Rac1, and inhibition of Rab11 function partially rescues E-cadherin destabilization. Thus, Rac1 activation reduces surface cadherin levels as a net result of higher bulk flow of membrane uptake that counteracts Rab11-dependent E-cadherin delivery to junctions (recycling and/or exocytosis). This unique small GTPase crosstalk has an impact on Rac1 and PAK1 regulation of membrane remodeling during epithelial dedifferentiation, adhesion, and motility.


Assuntos
Junções Aderentes/fisiologia , Exocitose , Queratinócitos/fisiologia , Quinases Ativadas por p21/metabolismo , Proteínas rab de Ligação ao GTP/metabolismo , Proteínas rac1 de Ligação ao GTP/metabolismo , Actinas/metabolismo , Junções Aderentes/química , Células Cultivadas , Humanos , Queratinócitos/citologia , Transdução de Sinais , Quinases Ativadas por p21/genética , Proteínas rab de Ligação ao GTP/genética , Proteínas rac1 de Ligação ao GTP/genética
7.
Mymensingh Med J ; 30(2): 351-354, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33830113

RESUMO

Chronic obstructive pulmonary disease (COPD) is a major public health problem which causes high health care utilizations, poor health-related quality of life, and substantial cost burden and deaths in worldwide. This study was done to evaluate the changes of peak expiratory flow rate (PEFR) in COPD patients in comparison to healthy person. This analytical type of cross sectional study was carried out in the Department of Physiology, Mymensingh Medical College, Mymensingh, Bangladesh from July 2018 to June 2019. A total number of 160 male subjects, age ranged from 30-70 years was included in this study. Among them, eighty (80) male COPD subjects were taken as study group (Group II) and eighty (80) age matched male healthy subjects were taken as control group (Group I). PEFR was assessed by peak flow meter. Data were expressed as mean ±SD and statistical significance of difference among the group was calculated by unpaired students' 't' test. The mean ±SD of PEFR of Group I and Group II were 432.75±30.23 L/min & 203.5±20.81 L/min respectively. The mean ±SD of PEFR significantly lower in study group in comparison with control group. Patients with COPD with greater changes in PEFR causes more frequent hospitalization and increased mortality and morbidity due to COPD related complications. So, assessment of this parameter is important for early detection and prevention of complications related to COPD for leading a healthy life.


Assuntos
Doença Pulmonar Obstrutiva Crônica , Qualidade de Vida , Adulto , Idoso , Bangladesh/epidemiologia , Estudos Transversais , Humanos , Masculino , Pessoa de Meia-Idade , Pico do Fluxo Expiratório , Doença Pulmonar Obstrutiva Crônica/diagnóstico , Doença Pulmonar Obstrutiva Crônica/epidemiologia
8.
Blood Adv ; 5(5): 1576-1584, 2021 03 09.
Artigo em Inglês | MEDLINE | ID: mdl-33687431

RESUMO

Ischemic stroke is a leading cause of morbidity and mortality worldwide and, despite reperfusion either via thrombolysis or thrombectomy, stroke patients often suffer from lifelong disabilities. These persistent neurological deficits may be improved by treating the ischemia/reperfusion (I/R) injury that occurs following ischemic stroke. There are currently no approved therapies to treat I/R injury, and thus it is imperative to find new targets to decrease the burden of ischemic stroke and related diseases. Platelets, cell fragments from megakaryocytes, are primarily known for their role in hemostasis. More recently, investigators have studied the nonhemostatic role of platelets in inflammatory pathologies, such as I/R injury after ischemic stroke. In this review, we seek to provide an overview of how I/R can lead to platelet activation and how activated platelets, in turn, can exacerbate I/R injury after stroke. We will also discuss potential mechanisms by which platelets may ameliorate I/R injury.


Assuntos
Traumatismo por Reperfusão , Acidente Vascular Cerebral , Plaquetas , Humanos , Isquemia , Ativação Plaquetária
9.
Braz J Biol ; 81(4): 940-953, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33605364

RESUMO

Nowadays food borne illness is most common in people due to their epidemic nature. These diseases affect the human digestive system through bacteria, viruses and parasites. The agents of illness are transmitted in our body through various types of food items, water and uncooked. Pathogens show drastic changes in immunosuppressant people. This review gives general insights to harmful microbial life. Pakistan is a developed country and because of its improper food management, a lot of gastrointestinal problems are noted in many patients. Bacteria are most common agents to spread diarrhoea, villi infection, constipation and dysenteric disease in human and induce the rejection of organ transplant. Enhancement of their lifestyle, properly cooked food should be used and to overcome the outbreak of the diseases.


Assuntos
Doenças Transmitidas por Alimentos , Bactérias , Diarreia , Doenças Transmitidas por Alimentos/epidemiologia , Humanos , Paquistão/epidemiologia
10.
Mymensingh Med J ; 30(1): 56-61, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33397851

RESUMO

Hypertension is an important worldwide public-health challenge because of its high frequency and concomitant risks of cardiovascular and kidney disease. It is recognized as a major contributor to the disease burden globally. Aim of the study was to evaluate body mass index and fasting serum glucose level in male hypertensive patients in order to compare these parameters with male normotensive subjects. This analytical type of cross sectional study was carried out in the Department of Physiology, Mymensingh Medical College, Bangladesh from July 2018 to June 2019. Total 200 male subjects, age range was from 30-65 years were included in this study. Among them, 100 hypertensive subjects were taken as study group (Group II) and 100 ages matched normotensive male subjects were taken as control group (Group I). Quantitative data were expressed as mean (±SD) and statistical significance of difference among the group was calculated by unpaired student's 't' test. In this study we found that body mass index was significantly (p<0.001) increased in study group (29.52±2.46) in comparison with control group (22.91±1.04). Fasting serum glucose level was also significantly (p<0.001) increased in study group (6.35±0.47) in comparison with control group (4.52±0.50). Based on the study carried out it is concluded that there is definite relationship of body mass index and fasting serum glucose level with hypertension. Therefore, basic measurement of weight and height to determine the body mass index and fasting serum glucose level as routine assessment during hospital visitation with appropriate lifestyle modification would help in controlling hypertension as well as its devastating consequences.


Assuntos
Jejum , Hipertensão , Adulto , Idoso , Bangladesh/epidemiologia , Glicemia , Índice de Massa Corporal , Estudos Transversais , Glucose , Humanos , Hipertensão/epidemiologia , Masculino , Pessoa de Meia-Idade
11.
Mymensingh Med J ; 30(1): 69-72, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33397853

RESUMO

Pregnancy is a natural physiological state with hormonal and metabolic changes that helps the growth and survival of the fetus. Adequate body mass index (BMI) in pregnancy is important for securing, protecting and promoting the health of women and newborns. High or low maternal body mass index is associated with adverse outcome. Thyroid physiology plays a major role in pregnancy and thyroid disorders constitute one of the most common endocrine disorders in pregnancy. To assess the levels of body mass index and serum thyroid stimulating hormone (TSH) in third trimester of pregnancy in order to compare these parameters with non-pregnant women of same age range. This analytical type of cross sectional study was carried out in the Department of Physiology, Mymensingh Medical College, Bangladesh from January 2019 to December 2019. A total number of 140 subjects, age range between 20-35 years were included in this study. Among them, 70 healthy subjects were taken as control group (Group I) and 70 pregnant women of third trimester were taken as study group (Group II). The results were calculated and analyzed by using SPSS. Quantitative data were expressed as mean (±SE) and statistical significance of difference among the group was calculated by unpaired student's 't' test. In this study we found that BMI was 27.87±1.9kg/m² and serum TSH level was 2.27±1.18µIU/ml in study group. Both (BMI & TSH) are significantly increased in study groups in comparison with control group. Therefore, by this study we recommended that routine estimation of these parameters is important for prevention of complication related to pregnancy.


Assuntos
Tireotropina , Adulto , Bangladesh , Índice de Massa Corporal , Estudos Transversais , Feminino , Humanos , Recém-Nascido , Gravidez , Terceiro Trimestre da Gravidez , Adulto Jovem
12.
Braz. J. Biol. ; 81(4): 99-103, out.-dez. 2021. graf, tab, ilus
Artigo em Inglês | VETINDEX | ID: vti-31535

RESUMO

Abstract Nowadays food borne illness is most common in people due to their epidemic nature. These diseases affect the human digestive system through bacteria, viruses and parasites. The agents of illness are transmitted in our body through various types of food items, water and uncooked. Pathogens show drastic changes in immunosuppressant people. This review gives general insights to harmful microbial life. Pakistan is a developed country and because of its improper food management, a lot of gastrointestinal problems are noted in many patients. Bacteria are most common agents to spread diarrhoea, villi infection, constipation and dysenteric disease in human and induce the rejection of organ transplant. Enhancement of their lifestyle, properly cooked food should be used and to overcome the outbreak of the diseases.(AU)


Resumo Hoje em dia, as doenças transmitidas por alimentos são mais comuns em pessoas devido à sua natureza epidêmica. Essas doenças afetam o sistema digestivo humano por meio de bactérias, vírus e parasitas. Os agentes das doenças são transmitidos em nosso corpo por meio de diversos tipos de alimentos, água e crus. Os patógenos mostram mudanças drásticas em pessoas imunossupressoras. Esta revisão fornece uma visão geral da vida microbiana prejudicial. O Paquistão é um país desenvolvido e, devido ao seu manejo alimentar inadequado, muitos problemas gastrointestinais são observados em muitos pacientes. As bactérias são os agentes mais comuns para espalhar diarreia, infecção de vilosidades, obstipação e doença disentérica em humanos e induzem a rejeição de transplantes de órgãos. Melhoria de seu estilo de vida, alimentos devidamente cozidos devem ser utilizados e para superar o aparecimento de doenças.(AU)


Assuntos
Parasitologia de Alimentos , Microbiologia de Alimentos , Prevenção de Doenças
13.
Mymensingh Med J ; 29(4): 859-864, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33116088

RESUMO

With the advancement of age, the production, metabolism and action of thyroid hormone are also changed. The incidence of thyroid dysfunction is increased greatly with age. Aim of the study was to assess the age-related change in thyroid function status in elderly male in order to compare it with younger male. This analytical type of cross-sectional study was carried out in the Department of Physiology, Mymensingh Medical College, Mymensingh, Bangladesh from January 2019 to December 2019. A total number of 140 male subjects were included in this study. Among them seventy (70) younger male were taken as control group (Group I) and seventy (70) elderly male were taken as study group (Group II). For assessment of thyroid function, serum free tri-iodothyronine (FT3), free thyroxine (FT4) and thyroid stimulating hormone (TSH) levels were estimated by RIA method. Data were expressed as mean (±SD) and statistical significance of difference among the group was calculated by unpaired student's t-test. Pearson's correlation coefficient test was done to find the correlation of serum FT3, FT4 & TSH with age. In this study we found that mean serum free tri-iodothyronine (FT3) & free thyroxine (FT4) levels were significantly lower and serum thyroid stimulating hormone (TSH) level was significantly higher in elderly male persons in comparison to younger male. Again, serum FT3, FT4 was negatively correlated with age of the subjects whereas serum TSH level was positively correlated with age of the subjects. Based on the study carried out it is concluded that there is a progressive decrease in thyroid function with advancement of age.


Assuntos
Testes de Função Tireóidea , Tireotropina , Idoso , Bangladesh , Estudos Transversais , Humanos , Masculino , Tiroxina , Tri-Iodotironina
14.
J Thromb Haemost ; 18(11): 3013-3028, 2020 11.
Artigo em Inglês | MEDLINE | ID: mdl-32767736

RESUMO

BACKGROUND: Immune complexes (ICs) bind to and activate platelets via FcγRIIA, causing patients to experience thrombocytopenia, as well as an increased risk of forming occlusive thrombi. Although platelets have been shown to mediate IC-induced pathologies, the mechanisms involved have yet to be fully elucidated. We identified that apoptosis signal-regulating kinase 1 (ASK1) is present in both human and mouse platelets and potentiates many platelet functions. OBJECTIVES: Here we set out to study ASK1's role in regulating IC-mediated platelet functions in vitro and IC-induced pathologies using an in vivo mouse model. METHODS: Using human platelets treated with an ASK1-specific inhibitor and platelets from FCGR2A/Ask1-/- transgenic mice, we examined various platelet functions induced by model ICs in vitro and in vivo. RESULTS: We found that ASK1 was activated in human platelets following cross-linking of FcγRIIA using either anti-hCD9 or IV.3 + goat-anti-mouse. Although genetic deletion or inhibition of ASK1 significantly attenuated anti-CD9-induced platelet aggregation, activation of the canonical FcγRIIA signaling targets Syk and PLCγ2 was unaffected. We further found that anti-mCD9-induced cPla2 phosphorylation and TxA2 generation is delayed in Ask1 null transgenic mouse platelets leading to diminished δ-granule secretion. In vivo, absence of Ask1 protected FCGR2A transgenic mice from thrombocytopenia, thrombosis, and systemic shock following injection of anti-mCD9. In whole blood microfluidics, platelet adhesion and thrombus formation on fibrinogen was enhanced by Ask1. CONCLUSIONS: These findings suggest that ASK1 inhibition may be a potential target for the treatment of IC-induced shock and other immune-mediated thrombotic disorders.


Assuntos
Trombocitopenia , Trombose , Animais , Apoptose , Plaquetas , Humanos , MAP Quinase Quinase Quinase 5/genética , Camundongos , Ativação Plaquetária , Agregação Plaquetária , Trombocitopenia/genética , Trombose/genética
15.
Environ Sci Pollut Res Int ; 27(4): 4460-4467, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31832957

RESUMO

Oil extraction is one of the causes of soil contamination with the total petroleum hydrocarbons. The objective of this study was to clarify the effect of Asteraceae plants on the degradation of petroleum hydrocarbon in contaminated soil. Initial soils with 40 and 90 g kg-1 of total petroleum hydrocarbon (TPH) were prepared. There were three treatments: (1) no addition, (2) addition of FeCl3 and nitrilotriacetic acid (NTA) solution, and (3) addition of FeCl3 + NTA and the cultivation of nine Asteraceae plants. The concentration of TPH was measured using infrared spectrophotometer, 2 and 3 months after transplanting (MAT). Shoot and root dry weights were measured 3 MAT. The concentration of TPH in soil cultivated with Cosmos caudatus was lower than that of the initial soil (40 g kg-1 TPH), 2 MAT. The concentrations of TPH in soils cultivated with Calendula officinalis, Callistephus chinensis, C. caudatus, and Tagetes sp. were also lower than that in the initial soil, 3 MAT. The concentrations of TPH in soils cultivated with Achillea filipendulina, Anthemis tinctoria, Tagetes erecta, Chrysanthemum coronarium, C. officinalis, C. chinensis, and C. caudatus were lower than that in the initial soil (90 g kg-1 TPH), 2 MAT. The concentrations of TPH in soils cultivated with T. erecta, A. tinctoria, Zinnia elegans, C. chinensis, C. caudatus, and Tagetes sp. were lower than that in the initial soil, 3 MAT. A. filipendulina and C. coronarium died at both 40 and 90 kg-1 TPH soils. These results suggest that the roots of Asteraceae plants degrade petroleum hydrocarbon in contaminated soil and C. chinensis and Z. elegans are more suitable for using TPH remediation. Plant survival and extensive root system are important factors for the remediation of TPH in contaminated soil.


Assuntos
Aster/química , Petróleo , Poluentes do Solo , Biodegradação Ambiental , Hidrocarbonetos/química , Petróleo/análise , Microbiologia do Solo , Poluentes do Solo/análise , Poluentes do Solo/química
16.
J Biol Chem ; 294(51): 19565-19576, 2019 12 20.
Artigo em Inglês | MEDLINE | ID: mdl-31719145

RESUMO

Endothelial nitric oxide (NO) synthase (eNOS) plays a critical role in the maintenance of blood vessel homeostasis. Recent findings suggest that cytoskeletal dynamics play an essential role in regulating eNOS expression and activation. Here, we sought to test whether modulation of cytoskeletal dynamics through pharmacological regulation of histone deacetylase 6 (HDAC6)-mediated tubulin deacetylation affects eNOS expression and endothelial function in vitro and in vivo We found that tubulin acetylation inducer (tubacin), a compound that appears to selectively inhibit HDAC6 activity, dramatically increased eNOS expression in several different endothelial cell lines, as determined by both immunoblotting and NO production assays. Mechanistically, we found that these effects were not mediated by tubacin's inhibitory effect on HDAC6 activity, but rather were due to its ability to stabilize eNOS mRNA transcripts. Consistent with these findings, tubacin also inhibited proinflammatory cytokine-induced degradation of eNOS transcripts and impairment of endothelium-dependent relaxation in the mouse aorta. Furthermore, we found that tubacin-induced up-regulation in eNOS expression in vivo is associated with improved endothelial function in diabetic db/db mice and with a marked attenuation of ischemic brain injury in a murine stroke model. Our findings indicate that tubacin exhibits potent eNOS-inducing effects and suggest that this compound might be useful for the prevention or management of endothelial dysfunction-associated cardiovascular diseases.


Assuntos
Anilidas/farmacologia , Endotélio Vascular/patologia , Desacetilase 6 de Histona/metabolismo , Inibidores de Histona Desacetilases/farmacologia , Ácidos Hidroxâmicos/farmacologia , Óxido Nítrico Sintase Tipo III/metabolismo , Óxido Nítrico/química , Acetilação , Animais , Aorta/metabolismo , Encéfalo/patologia , Isquemia Encefálica/patologia , Modelos Animais de Doenças , Feminino , Regulação Enzimológica da Expressão Gênica , Células Endoteliais da Veia Umbilical Humana , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Acidente Vascular Cerebral/fisiopatologia , Tubulina (Proteína)/química , Regulação para Cima
17.
Biochem J ; 476(19): 2835-2850, 2019 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-31530712

RESUMO

Apoptosis signal-regulating kinase 1 (ASK1) is a mitogen-activated protein kinase kinase kinase (MAPKKK) that regulates activation of the c-Jun N-terminal kinase (JNK)- and p38-stress response pathways leading to apoptosis in nucleated cells. We have previously shown that ASK1 is expressed in platelets and regulates agonist-induced platelet activation and thrombosis. However, the mechanism by which platelet agonists cause activation of ASK1 is unknown. Here, we show that in platelets agonist-induced activation of p38 is exclusively dependent on ASK1. Both thrombin and collagen were able to activate ASK1/p38. Activation of ASK1/p38 was strongly dependent on thromboxane A2 (TxA2) and ADP. Agonist-induced ASK1 activation is blocked by inhibition of phospholipase C (PLC) ß/γ activity or by chelating intracellular Ca2+. Furthermore, treatment of platelets with thapsigargin or Ca2+ ionophore robustly induced ASK1/p38 activation. In addition, calcium and integrin-binding protein 1 (CIB1), a Ca2+-dependent negative regulator of ASK1, associates with ASK1 in resting platelets and is dissociated upon platelet activation by thrombin. Dissociation of CIB1 corresponds with ASK1 binding to tumor necrosis factor (TNF) receptor associated factor 6 (TRAF6) and the autophosphorylation of ASK1 Thr838 within the catalytic domain results in full activation of ASK1. Furthermore, genetic ablation of Cib1 in mice augments agonist-induced Ask1/p38 activation. Together our results suggest that in resting platelets ASK1 is bound to CIB1 at low Ca2+ concentrations. Agonist-induced platelet activation causes an increase in intracellular Ca2+ concentration that leads to the dissociation of CIB1 from ASK1, allowing for proper dimerization through ASK1 N-terminal coiled-coil (NCC) domains.


Assuntos
Plaquetas/metabolismo , Proteínas de Ligação ao Cálcio/metabolismo , MAP Quinase Quinase Quinase 5/metabolismo , Ativação Plaquetária/fisiologia , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Animais , Plaquetas/citologia , Cálcio/metabolismo , Feminino , Voluntários Saudáveis , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Ligação Proteica
18.
Mymensingh Med J ; 27(3): 578-584, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30141449

RESUMO

Thalassaemia is one of the commonest inherited disorders in Bangladesh. Since population is rapidly increasing in Bangladesh, therefore birth of large number of new cases is expected every year. Chorionic villus sampling (CVS) is an invasive diagnostic procedure done in early pregnancy to obtain fetal cells for the prenatal diagnosis of chromosomal and genetic disorders like thalassemia. To investigate the feasibility, safety, and outcome of transabdominal Chorionic Villus Sampling (CVS) for prenatal diagnosis of Beta Thalassemia, this retrospective analytical study was carried out on women who had undergone transabdominal CVS for prenatal diagnosis of Beta thalassemia. All CVS are done at Fetal Medicine Centre, Family Foundation, Green Road, Dhaka, Bangladesh from June 2013 to December 2016. A total of 236 couples who were referred for prenatal diagnosis of thalassemia (Beta thalassemia & Hb-E beta thalassemia) were studied. Transabdominal CVS was done under local anesthesia and real-time ultrasound guidance. A 18G/88mm Spinal Needle (B Braun, Germany) was used. Most procedures were done between 11 and 13 weeks (range 11-14 weeks). All CVS were performed with the "2 operators" technique. The samples were sent for prenatal diagnosis by genetic analysis. Results were recorded and analyzed for descriptive statistics. Out of 236, 123 (52.1%) fetuses were diagnosed as trait (Beta trait or HbE trait), 64 (27.1%) as healthy fetuses and 49 (20.7%) as affected fetuses. A total of 236 CVSs were performed as outdoor basis. Most aspirations (95.1%) were easy; however, in 4.8% cases the aspiration was difficult due to a variety of factors. The overall success rate was 100%. Minor complications like placental hematoma and pervaginal (P/V) bleeding occurred in 2% & 1.3% respectively which were subsided by conservative management. The procedure related miscarriage within three weeks not occurred in any cases. Transabdominal CVS under real-time sonography is a useful outdoor procedure for prenatal diagnosis of beta thalassaemia in early pregnancy without significant risk to the mother and the fetus.


Assuntos
Amostra da Vilosidade Coriônica , Talassemia , Bangladesh , Feminino , Humanos , Gravidez , Diagnóstico Pré-Natal , Estudos Retrospectivos , Talassemia/diagnóstico
19.
G3 (Bethesda) ; 8(7): 2333-2343, 2018 07 02.
Artigo em Inglês | MEDLINE | ID: mdl-29748198

RESUMO

Convergent evolution is often due to selective pressures generating a similar phenotype. We observe relatively recent duplications in a spectrum of Saccharomycetaceae yeast species resulting in multiple phosphatases that are regulated by different nutrient conditions - thiamine and phosphate starvation. This specialization is both transcriptional and at the level of phosphatase substrate specificity. In Candida glabrata, loss of the ancestral phosphatase family was compensated by the co-option of a different histidine phosphatase family with three paralogs. Using RNA-seq and functional assays, we identify one of these paralogs, CgPMU3, as a thiamine phosphatase. We further determine that the 81% identical paralog CgPMU2 does not encode thiamine phosphatase activity; however, both are capable of cleaving the phosphatase substrate, 1-napthyl-phosphate. We functionally demonstrate that members of this family evolved novel enzymatic functions for phosphate and thiamine starvation, and are regulated transcriptionally by either nutrient condition, and observe similar trends in other yeast species. This independent, parallel evolution involving two different families of histidine phosphatases suggests that there were likely similar selective pressures on multiple yeast species to recycle thiamine and phosphate. In this work, we focused on duplication and specialization, but there is also repeated loss of phosphatases, indicating that the expansion and contraction of the phosphatase family is dynamic in many Ascomycetes. The dynamic evolution of the phosphatase gene families is perhaps just one example of how gene duplication, co-option, and transcriptional and functional specialization together allow species to adapt to their environment with existing genetic resources.


Assuntos
Família Multigênica , Fosfatos/metabolismo , Monoéster Fosfórico Hidrolases/genética , Monoéster Fosfórico Hidrolases/metabolismo , Tiamina/metabolismo , Leveduras/fisiologia , Candida glabrata/fisiologia , Meio Ambiente , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Duplicação Gênica , Regulação Fúngica da Expressão Gênica , Sequenciamento de Nucleotídeos em Larga Escala , Hidrólise , Filogenia , Saccharomycetales/fisiologia , Especificidade por Substrato , Leveduras/classificação
20.
Ecol Evol ; 8(24): 12737-12749, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30619578

RESUMO

The ecological implications of body size extend from the biology of individual organisms to ecosystem-level processes. Measuring body mass for high numbers of invertebrates can be logistically challenging, making length-mass regressions useful for predicting body mass with minimal effort. However, standardized sets of scaling relationships covering a large range in body length, taxonomic groups, and multiple geographical regions are scarce. We collected 6,212 arthropods from 19 higher-level taxa in both temperate and tropical locations to compile a comprehensive set of linear models relating live body mass to a range of predictor variables. We measured live weight (hereafter, body mass), body length and width of each individual and conducted linear regressions to predict body mass using body length, body width, taxonomic group, and geographic region. Additionally, we quantified prediction discrepancy when using parameters from arthropods of a different geographic region. Incorporating body width into taxon- and region-specific length-mass regressions yielded the highest prediction accuracy for body mass. Using regression parameters from a different geographic region increased prediction discrepancy, causing over- or underestimation of body mass depending on geographical origin and whether body width was included. We present a comprehensive range of parameters for predicting arthropod body mass and provide guidance for selecting optimal scaling relationships. Given the importance of body mass for functional invertebrate ecology and the paucity of adequate regressions to predict arthropod body mass from different geographical regions, our study provides a long-needed resource for quantifying live body mass in invertebrate ecology research.

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