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1.
Braz J Biol ; 83: e269317, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36722663

RESUMO

Bacteria may be the initial cause of certain pathologies as well as a secondary agent responsible for the development of complications such as pressure ulcer infections. Pressure ulcers are a persistent health problem, especially in immunocompromised patients, and associated with infection by opportunistic microorganisms with antimicrobial resistance, such as Klebsiella pneumoniae, highlight the need for the development of new antimicrobial approaches. Thus, the aim of this study was to evaluate the antibacterial and anti-adherent activity of Origanum vulgare L. (oregano) essential oil against Klebsiella pneumoniae strains, as well as the effect of its association with synthetic antimicrobials. To this end, the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) analyses were performed on microdilution plates. The assay of the Minimum Inhibitory Adherence Concentration (MIAC), with test tubes. As well as, the association study through the infusion disc method containing ampicillin (AMP), gentamicin (GEN), ciprofloxacin (CIP) and ceftriaxone (CEF). Therefore, it was possible to obtain that the essential oil of oregano presents antimicrobial and bactericidal activity, with MIC ranging between 128µg/mL and 256 µg/mL and MBC between 256 µg/mL and 512 µg/mL, on the tested K. pneumoniae strains. When used in association with ampicillin and gentamicin, oregano essential oil showed synergistic effect for some strains. Therefore, it is observed that the tested essential oil can act as a promising antibacterial in the treatment of diseases caused by K. pneumoniae.


Assuntos
Óleos Voláteis , Origanum , Humanos , Klebsiella pneumoniae , Antibacterianos/farmacologia , Ampicilina , Gentamicinas , Óleos Voláteis/farmacologia
2.
Arq. bras. med. vet. zootec. (Online) ; 73(6): 1403-1412, Nov.-Dec. 2021. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1355685

RESUMO

The objective was to identify lowering strategies for Urochloa brizantha cv. Marandu in the beginning of the stockpiling period that increase forage production and improve the structure of stockpiled canopies. Three lowering strategies were evaluated: maintenance of marandu palisade grass with 15cm four months before the stockpiling period; maintenance of palisade grass with 30cm for four months, and lowering to 15cm before the stockpiling period; and maintaining of palisade grass at 45cm for four months, and lowering to 15cm before the stockpiling period. The experimental design was completely randomized, with four replications. At the end of stockpiling, the number of reproductive tillers and forage mass were higher in the canopy under 15/15cm than in the others; the percentage of live leaf was higher in the canopy under 45/15cm than under 15/15cm. Forage production during the stockpiling period was higher in the canopy under 15/15cm and lower in the under 45/15cm. Maintaining 15cm marandu palisadegrass for four months before the stockpiling period increases forage production during this period. The lowering of the marandu palisadegrass from 45 to 15cm at the beginning of the stockpiling period improves the structure of the stockpiled canopy.(AU)


Objetivou-se identificar estratégias de rebaixamento da Urochloa brizantha cv. Marandu no início do diferimento, as quais resultem em aumento da produção e em melhoria da estrutura do dossel diferido. Foram avaliadas três estratégias de rebaixamento no início do diferimento: manutenção do capim-marandu com 15cm por quatro meses antes do início do diferimento; manutenção do capim-marandu com 30cm por quatro mese, e rebaixamento para 15cm no início do diferimento; e manutenção do capim com 45cm por quatro meses e rebaixamento para 15cm no início do diferimento. O delineamento foi inteiramente ao acaso, com quatro repetições. No fim do diferimento, o número de perfilho reprodutivo e a massa de forragem foram superiores no dossel sob 15/15cm do que nos demais; a percentagem de folha viva foi maior no dossel sob 45/15cm do que sob 15/15cm. A produção de forragem durante o diferimento foi superior no dossel sob 15/15cm e inferior no sob 45/15cm. A manutenção do capim-marandu com 15cm por quatro meses antes do período de diferimento aumenta a produção de forragem neste período. O rebaixamento do capim-marandu de 45 para 15cm no início do diferimento melhora a estrutura do dossel diferido.(AU)


Assuntos
Cultivos Agrícolas , Folhas de Planta/anatomia & histologia , Brachiaria/crescimento & desenvolvimento
3.
Arq. bras. med. vet. zootec. (Online) ; 73(6): 1413-1421, Nov.-Dec. 2021. tab, graf
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1355684

RESUMO

The objective this study was to identify differences in the morphogenic patterns of four Urochloa brizantha cultivars (marandu, piatã, xaraés and paiaguás grasses) during the stockpiling period. A completely randomized design was used, with three replications, in experimental plots of 9m². The evaluations took place over 2 years. The grasses were stockpiled for 92 (Year 1) and 95 (Year 2) days. The leaf appearance rate of paiaguás grass was higher, compared to other grasses. In Year 1, the stem elongation rate of xaraés grass was higher than other grasses. At the end of stockpiling period of Year 1, the tiller population density (TPD) was higher in the paiaguás grass, intermediate in the xaraés and marandu grasses and lower in the piatã grass. At the end of the stockpiling period in Year 2, TPD was higher in the paiaguás grass canopy, intermediate in the marandu and piatã grasses canopies, and lower in the xaraés grass canopy. Paiaguás grass has greater leaf growth during the stockpiling period and is therefore suitable for use under stockpiled grazing. Xaraés grass has high stem elongation during the stockpiling period, which is why its use under stockpiled grazing must be accompanied by adjustments in pasture management.(AU)


O objetivo deste estudo foi identificar diferenças nos padrões morfogênicos de quatro cultivares de Urochloa brizantha (marandu, piatã, xaraés e paiaguás) durante o período de diferimento. Foi utilizado o delineamento inteiramente ao acaso, com três repetições, em parcelas experimentais de 9m². As avaliações ocorreram por dois anos. Os capins foram diferidos por 92 (Ano 1) e 95 (Ano 2) dias. A taxa de aparecimento foliar do capim-paiaguás foi maior, em comparação aos demais capins. No Ano 1, a taxa de alongamento do colmo do capim-xaraés foi superior aos demais capins. No final do diferimento do Ano 1, a densidade populacional de perfilho (DPP) foi maior no capim-paiaguás, intermediária nos capins xaraés e marandu e inferior no capim-piatã. No fim do período de diferimento do Ano 2, a DPP foi superior no dossel de capim-paiaguás, intermediária nos dosséis dos capins marandu e piatã, e menor no dossel de capim-xaraés. O capim-paiaguás tem maior crescimento foliar durante o período de diferimento, sendo, portanto, apropriado para uso sob pastejo diferido. O capim-xaraés apresenta elevado alongamento de colmo durante o período de diferimento, razão pela qual seu uso sob pastejo diferido deve vir acompanhado de ajustes no manejo da pastagem.(AU)


Assuntos
Caules de Planta , Brachiaria/crescimento & desenvolvimento , Morfogênese/fisiologia
4.
Braz. J. Vet. Pathol. ; 14(1): 66-69, mar. 2021. ilus
Artigo em Inglês | VETINDEX | ID: vti-31224

RESUMO

An approximately 3-month-old crab-eating fox (Cerdocyon thous) was found by environmental authorities in the State of Paraiba, Northeastern Brazil and referred to a wildlife care center. The fox was presenting respiratory distress and it was referred to the Veterinary Hospital of the Federal University of Paraiba (UFPB) for ancillary testing. Abdominal and thoracic ultrasound and radiographies were performed. These imaging tests indicated the fox had a possible diaphragmatic hernia and ectopic kidney. The imageology results were confirmed on necropsy, which revealed a postero-lateral focal discontinuity of the dorsal aspect of the diaphragmatic muscle with protrusion of the gastrointestinal tract into the thoracic cavity. The stomach and intestinal loops were filled with gas and obliterated the visualization of the heart and lungs. Additionally, only the right kidney was found, and no vestigial left kidney was identified. Congenital diaphragmatic hernias are not commonly observed in wildlife but should be considered as a potential differential diagnosis for acute onset of respiratory distress in young carnivores.(AU)


Assuntos
Animais , Hérnia Diafragmática/classificação , Hérnia Diafragmática/diagnóstico , Rim Único/classificação , Rim Único/diagnóstico , Raposas , Xiphosura americana
5.
Int J Pharm ; 591: 120001, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-33141086

RESUMO

The aim of this study was to develop and characterize a double layer biomembrane for dual drug delivery to be used for the treatment of wounds. The membrane was composed of chitosan, hydroxypropyl methylcellulose and lidocaine chloride (anesthetic drug) in the first layer, and of sodium alginate-polymyxin B sulphate (antibiotic) nanoparticles as the second layer. A product with excellent thickness (0.01-0.02 mm), adequate mechanical properties with respect to elasticity, stiffness, tension, and compatible pH for lesion application has been successfully obtained. The incorporation of the drugs was confirmed analysing the membrane cross-sections by scanning electron microscopy. A strong interaction between the drugs and the functional groups of respective polymers was confirmed by Fourier-Transform Infrared Spectroscopy, thermal analysis and X-ray diffraction. Microbiological assays showed a high antimicrobial activity when polymyxin B was present to act against the Staphylococcus aureus and Pseudomonas aeruginosa strains. Low cytotoxicity observed in a cell viability colorimetric assay and SEM analysis suggest biocompatibility between the developed biomembrane and the cell culture. The in vivo assay allowed visualizing the healing potential by calculating the wound retraction index and by histological analysis. Our results confirm the effectiveness of the developed innovative biomaterial for tissue repair and regeneration in an animal model.


Assuntos
Quitosana , Nanopartículas , Alginatos , Animais , Bandagens , Lidocaína , Polimixinas , Espectroscopia de Infravermelho com Transformada de Fourier , Cicatrização
6.
Int J Biol Macromol ; 140: 761-770, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31434004

RESUMO

Lipase B from Candida antarctica (CalB) is the most widely used lipase, including in many industrial sectors, such as in biodiesel and pharmaceuticals production. CalB has been produced by heterologous expression using Pichia pastoris under PGK constitutive promoter (named LipB). Here, we have studied the structural features of commercial CalB and LipB enzymes using circular dichroism and fluorescence under different conditions. In the presence of denaturing agents CalB was more stable than LipB, in contrast, at increasing temperatures, LipB was more thermostable than CalB. Mass spectrometry data indicates that both enzymes have an insertion of amino acids related to α-factor yeast signal, however LipB enzyme showed the addition of nine residues at the N-terminal while CalB showed only four residues. Molecular modeling of LipB showed the formation of an amphipathic α-helix in N-terminal region that was not observed in CalB. This data suggests that this new α-helix possess could be involved in LipB thermostability. These results associated with new structural studies may provide information to the design of novel biocatalysts.


Assuntos
Candida/enzimologia , Proteínas Fúngicas/química , Lipase/química , Proteínas Recombinantes de Fusão , Sequência de Aminoácidos , Candida/genética , Ativação Enzimática , Estabilidade Enzimática , Proteínas Fúngicas/genética , Proteínas Fúngicas/isolamento & purificação , Proteínas Fúngicas/metabolismo , Hidrólise , Lipase/genética , Lipase/isolamento & purificação , Lipase/metabolismo , Modelos Moleculares , Conformação Proteica , Relação Estrutura-Atividade , Temperatura , Termodinâmica
7.
B. Indústr. Anim. ; 752018. ilus, tab
Artigo em Português | VETINDEX | ID: vti-738944

RESUMO

The objective of this study was to evaluate the incidence of bruises and losses due to injuries in bovine carcasses submitted to road transport. A total of 1599 carcasses were evaluated for the number of bruises and 1440 for injuries losses. The study was conducted in the city of Aquidauana/MS, at a slaughterhouse. The number, location, color and size of the bruises were visually assessed. In addition, data regarding types of trucks in which the animals were transported, distance traveled to the slaughterhouse, sexual class and age of the animals were collected. In order to quantify the injury losses, the injuries removed during toilet of the carcasses were collected and weighed. Animals that traveled longer distances to the slaughterhouse showed more bruises in the carcasses, 97.81% of the carcasses evaluated had one or more bruises. In addition, the regions of the carcasses that obtained the highest average percentage of bruises were the sacral and lumbar, being 31.77 and 31.63%, respectively. Bruises in the posterior area of the carcass may represent greater economic losses because meat cuts of higher market value are located in this area. Females and uncastrated males were the groups with the highest number of carcasses with bruises. The injuries represented an average loss of 0.225 kg per carcass, generating an average economic loss of R$ 2.14, in this context, females showed greater losses due to injuries when compared to uncastrated and castrated males. In conclusion, longer distances increase the risk of bruised carcasses. Transport with inadequate stocking, physical conditions of vehicles and inadequate handling are the main causes of bruising. In addition, factors inherent to the animals may also favor the occurrence of bruises and injuries, such as lower fat cover in females and the more aggressive behavior of uncastrated males when compared to castrated males.(AU)


Objetivou-se avaliar a incidência de hematomas e perdas por lesões em carcaças bovinas de animais submetidos ao transporte rodoviário. Foram avaliadas 1.599 carcaças quanto ao número de hematomas e 1.440 quanto as perdas por lesões. O estudo foi conduzido no município de Aquidauana/MS, em abatedouro frigorífico. Avaliou-se visualmente o número, localização, coloração e tamanho dos hematomas. Além disso, foram coletados dados referentes aos tipos de caminhões em que os animais foram transportados, distância percorrida até o abatedouro frigorífico, classe sexual e idade dos animais. Para quantificar as lesões, realizou-se a coleta e pesagem das lesões retiradas durante a toalete das carcaças. Animais que percorreram maiores distâncias até o frigorífico apresentaram maior número de lesões nas carcaças, sendo que, 97,81% das carcaças avaliadas, apresentaram um ou mais hematomas. Além disso, a região sacral e lombar foram as que obtiveram a maior percentagem média de hematomas, 31,77 e 31,63%, respectivamente. Hematomas na área posterior da carcaça podem representar maiores perdas econômicas pois cortes cárneos de maior valor agregado se localizam nessa área. Fêmeas e machos inteiros foram os grupos com maior número de carcaças com hematomas. As lesões representaram uma perda média de 0,225 kg por carcaça, gerando uma perda econômica média de R$ 2,14, sendo que as fêmeas apresentaram maior perda por lesões quando comparadas a machos inteiros e castrados. Em distâncias mais longas o risco de surgirem animais contundidos aumenta. Transporte com lotação inadequada, condições físicas dos veículos de transporte e manejos inadequados são as principais causas de ocorrência de hematomas. Além destes, fatores inerentes aos animais também podem favorecer a ocorrência de hematomas e lesões como, por exemplo, menor acabamento de gordura na carcaça em fêmeas e o comportamento mais agressivo de machos inteiros quando comparados a machos castrados.(AU)


Assuntos
Animais , Bovinos , Ferimentos e Lesões/veterinária , Meios de Transporte , Abate de Animais/economia , Carne Vermelha/análise , Bem-Estar do Animal , Qualidade dos Alimentos
8.
Braz. j. biol ; 76(3): 645-655, July-Sept. 2016. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-25330

RESUMO

Considering the physiological importance and need of greater morphophysiological knowledge of adrenal glands, the aims of present study were compare the morphometric data between left and right adrenal of male and female; perform a histological, scanning and transmission electron microscopy study showing tissue constitution of glands; finally, in order to define the presence and correct site of the cytochrome P450c17 expression in adrenal glands, immunohistochemical study of this enzyme was performed in 18 adrenal glands (right n=9 and left n=9) of nine adult Galea spixii (four males and five females). Right adrenal was more cranially positioned than left adrenal; dimensions (weight, length and width) of right adrenal was larger than left adrenal; no differences between male and female body and adrenal measurements were found; the morphology of cells and different amounts of lipid droplets may be related to the different demands of steroid hormones production, related to each zone of the adrenal cortex; and, the cytochrome P450c17 immunolocalization in fasciculate and reticular zone may be related with synthesis of 17-hydroxy-pregnenolone, 17-hydroxy-progesterone, dehydroepiandrosterone or androstenedione.(AU)


Considerando a importância fisiológica e a necessidade de melhor conhecimento morfofisiológico das glândulas adrenais, os objetivos do presente estudo foram comparar dados morfométricos entre adrenais direitas e esquerdas de machos e fêmeas; realizar estudos histológicos e microscopia eletrônica de varredura e transmissão revelando a constituição tecidual das glândulas; finalmente, foi realizado estudo imunohistoquímico com objetivo de definir a presença e correto sítio de expressão da citocromo P450c17 nas glândulas adrenais em 18 glândulas adrenais (direita n=9 e esquerda n=9) de nove Galea spixii adultos (quatro machos e cinco fêmeas). A glândula adrenal direita estava posicionada mais cranialmente que a adrenal esquerda; as dimensões (peso, comprimento e largura) da glândula adrenal direita foram maiores que as da esquerda; não houve diferença entre as medidas corpóreas e das adrenais entre machos e fêmeas; a morfologia das células e diferentes quantidades de gotículas lipídicas podem estar relacionadas a diferentes demandas na produção de hormônios esteroides relacionados a cada zona do córtex adrenal; e, a imunolocalização da citocromo P450 na zona fasciculada e reticular pode estar relacionado com a síntese de 17-hidroxi-pregnenolona, 17-hidroxi-progesterona, dehidroepiandrosterona ou androstenediona.(AU)


Assuntos
Animais , Roedores/anatomia & histologia , Roedores/classificação , Glândulas Suprarrenais/anatomia & histologia
9.
Braz. j. biol ; 76(3): 645-655, tab, graf
Artigo em Inglês | LILACS | ID: lil-785027

RESUMO

Abstract Considering the physiological importance and need of greater morphophysiological knowledge of adrenal glands, the aims of present study were compare the morphometric data between left and right adrenal of male and female; perform a histological, scanning and transmission electron microscopy study showing tissue constitution of glands; finally, in order to define the presence and correct site of the cytochrome P450c17 expression in adrenal glands, immunohistochemical study of this enzyme was performed in 18 adrenal glands (right n=9 and left n=9) of nine adult Galea spixii (four males and five females). Right adrenal was more cranially positioned than left adrenal; dimensions (weight, length and width) of right adrenal was larger than left adrenal; no differences between male and female body and adrenal measurements were found; the morphology of cells and different amounts of lipid droplets may be related to the different demands of steroid hormones production, related to each zone of the adrenal cortex; and, the cytochrome P450c17 immunolocalization in fasciculate and reticular zone may be related with synthesis of 17-hydroxy-pregnenolone, 17-hydroxy-progesterone, dehydroepiandrosterone or androstenedione.


Resumo Considerando a importância fisiológica e a necessidade de melhor conhecimento morfofisiológico das glândulas adrenais, os objetivos do presente estudo foram comparar dados morfométricos entre adrenais direitas e esquerdas de machos e fêmeas; realizar estudos histológicos e microscopia eletrônica de varredura e transmissão revelando a constituição tecidual das glândulas; finalmente, foi realizado estudo imunohistoquímico com objetivo de definir a presença e correto sítio de expressão da citocromo P450c17 nas glândulas adrenais em 18 glândulas adrenais (direita n=9 e esquerda n=9) de nove Galea spixii adultos (quatro machos e cinco fêmeas). A glândula adrenal direita estava posicionada mais cranialmente que a adrenal esquerda; as dimensões (peso, comprimento e largura) da glândula adrenal direita foram maiores que as da esquerda; não houve diferença entre as medidas corpóreas e das adrenais entre machos e fêmeas; a morfologia das células e diferentes quantidades de gotículas lipídicas podem estar relacionadas a diferentes demandas na produção de hormônios esteroides relacionados a cada zona do córtex adrenal; e, a imunolocalização da citocromo P450 na zona fasciculada e reticular pode estar relacionado com a síntese de 17-hidroxi-pregnenolona, 17-hidroxi-progesterona, dehidroepiandrosterona ou androstenediona.


Assuntos
Animais , Masculino , Feminino , Roedores/anatomia & histologia , Glândulas Suprarrenais/anatomia & histologia , Cobaias
10.
Braz J Biol ; 76(3): 645-55, 2016 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-27143060

RESUMO

Considering the physiological importance and need of greater morphophysiological knowledge of adrenal glands, the aims of present study were compare the morphometric data between left and right adrenal of male and female; perform a histological, scanning and transmission electron microscopy study showing tissue constitution of glands; finally, in order to define the presence and correct site of the cytochrome P450c17 expression in adrenal glands, immunohistochemical study of this enzyme was performed in 18 adrenal glands (right n=9 and left n=9) of nine adult Galea spixii (four males and five females). Right adrenal was more cranially positioned than left adrenal; dimensions (weight, length and width) of right adrenal was larger than left adrenal; no differences between male and female body and adrenal measurements were found; the morphology of cells and different amounts of lipid droplets may be related to the different demands of steroid hormones production, related to each zone of the adrenal cortex; and, the cytochrome P450c17 immunolocalization in fasciculate and reticular zone may be related with synthesis of 17-hydroxy-pregnenolone, 17-hydroxy-progesterone, dehydroepiandrosterone or androstenedione.


Assuntos
Glândulas Suprarrenais/anatomia & histologia , Roedores/anatomia & histologia , Animais , Feminino , Cobaias , Masculino
11.
Braz. J. Biol. ; 76(3)2016.
Artigo em Inglês | VETINDEX | ID: vti-744769

RESUMO

Abstract Considering the physiological importance and need of greater morphophysiological knowledge of adrenal glands, the aims of present study were compare the morphometric data between left and right adrenal of male and female; perform a histological, scanning and transmission electron microscopy study showing tissue constitution of glands; finally, in order to define the presence and correct site of the cytochrome P450c17 expression in adrenal glands, immunohistochemical study of this enzyme was performed in 18 adrenal glands (right n=9 and left n=9) of nine adult Galea spixii (four males and five females). Right adrenal was more cranially positioned than left adrenal; dimensions (weight, length and width) of right adrenal was larger than left adrenal; no differences between male and female body and adrenal measurements were found; the morphology of cells and different amounts of lipid droplets may be related to the different demands of steroid hormones production, related to each zone of the adrenal cortex; and, the cytochrome P450c17 immunolocalization in fasciculate and reticular zone may be related with synthesis of 17-hydroxy-pregnenolone, 17-hydroxy-progesterone, dehydroepiandrosterone or androstenedione.


Resumo Considerando a importância fisiológica e a necessidade de melhor conhecimento morfofisiológico das glândulas adrenais, os objetivos do presente estudo foram comparar dados morfométricos entre adrenais direitas e esquerdas de machos e fêmeas; realizar estudos histológicos e microscopia eletrônica de varredura e transmissão revelando a constituição tecidual das glândulas; finalmente, foi realizado estudo imunohistoquímico com objetivo de definir a presença e correto sítio de expressão da citocromo P450c17 nas glândulas adrenais em 18 glândulas adrenais (direita n=9 e esquerda n=9) de nove Galea spixii adultos (quatro machos e cinco fêmeas). A glândula adrenal direita estava posicionada mais cranialmente que a adrenal esquerda; as dimensões (peso, comprimento e largura) da glândula adrenal direita foram maiores que as da esquerda; não houve diferença entre as medidas corpóreas e das adrenais entre machos e fêmeas; a morfologia das células e diferentes quantidades de gotículas lipídicas podem estar relacionadas a diferentes demandas na produção de hormônios esteroides relacionados a cada zona do córtex adrenal; e, a imunolocalização da citocromo P450 na zona fasciculada e reticular pode estar relacionado com a síntese de 17-hidroxi-pregnenolona, 17-hidroxi-progesterona, dehidroepiandrosterona ou androstenediona.

12.
Rev. bras. plantas med ; 17(3): 407-412, Jul-Sep/2015. tab, graf
Artigo em Português | LILACS | ID: lil-752563

RESUMO

RESUMO O presente trabalho teve por objetivo observar o uso de plantas medicinais por moradores de Quixadá-Ceará. A amostragem adotada foi 5% da população urbana adulta na sede do município, o que resultou em 263 domicílios visitados, onde foi aplicado um questionário semi-estruturado. De acordo com os resultados ainda temos um número expressivo de pessoas que fazem uso de plantas medicinais na cidade de Quixadá-Ceará (57,41%), e que a maioria dos entrevistados (97%) considera que o uso indiscriminado de plantas medicinais não causa nenhum problema à saúde. A maioria dos entrevistados foram mulheres (63,57%) e o maior número de informações e conhecimentos sobre plantas medicinais concentrou-se na faixa de 51-60 anos (24,71%). Com relação ao nível de escolaridade, 34,22% dos entrevistados afirmou ter ensino médio completo. A família botânica mais representativa foi a Lamiaceae (21,05%) e a espécie mais citada foi a erva-cidreira. Dos entrevistados 93,37% consideraram utilizar o chá como medicamento e 6,63% alegaram fazer a utilização do mesmo como alimento. Quanto à frequência do uso de plantas medicinais de forma contínua, 27% alegaram fazer o uso diariamente.


ABSTRACT This study aimed to observe the use of medicinal plants by residents from Quixada-Ceara. The sample used was 5% of the adult urban population in the county seat, which resulted in 263 households visited, where we applied a semi-structured questionnaire. According to the results, there is still a significant number of people who uses medicinal plants in the city of Quixada-Ceara (57.41%), and the majority of participants (97%) consider that the indiscriminate employment of medicinal plants do not cause any health problem. Most respondents were female (63.57%) and the highest number of information and knowledge about medicinal plants were concentrated among 51-60 years old individuals (24.71%). In regard of the educational level, 34.22% of the participants claimed to have completed high school. The botanical family Lamiaceae was the most representative (21.05%) and the most cited specie was the lemongrass. 93.37% of the respondents pledged to use tea as medicine and 6.63% claimed to use it as food. Regarding the employment frequency of the medicinal plants, 27% claimed to take them daily.


Assuntos
Humanos , Masculino , Feminino , Fitoterapia/estatística & dados numéricos , Plantas Medicinais/classificação , Botânica/métodos , Etnobotânica/métodos , População Urbana/estatística & dados numéricos
13.
Animal ; 9(2): 362-72, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25256316

RESUMO

The objective of this study was to evaluate the fatty acid profile and qualitative characteristics of meat from feedlot young bulls fed ground soybean or ground cottonseed, with or without supplementation of vitamin E. A total of 40 Red Norte young bulls, with an initial average age of 20 months, and an initial average BW of 339±15 kg, were allotted in a completely randomized design using a 2×2 factorial arrangement, with two oilseeds, and daily supplementation or not of 2500 IU of vitamin E. The experimental period was for 84 days, which was preceded by an adaptation period of 28 days. The treatments were ground soybean (SB), ground soybean plus vitamin E (SBE), ground cottonseed (CS) and ground cottonseed plus vitamin E (CSE). The percentage of cottonseed and soybean in the diets (dry matter basis) was 24% and 20%, respectively. Diets were isonitrogenous (13% CP) and presented similar amount of ether extract (6.5%). The animals were slaughtered at average live weight of 464±15 kg, and samples were taken from the longissimus dorsi muscle for the measurement of fatty acid concentration and the evaluation of lipid oxidation and color of the beef. Before fatty acid extraction, muscle tissue and subcutaneous fat of the longissimus dorsi were separated to analyze fatty acid profile in both tissues. Supplementation of vitamin E did not affect fatty acid concentration, lipid oxidation and color (P>0.05). Subcutaneous fat from animals fed CS diet had greater C12:0, C16:0 and C18:0 contents (P<0.03). In addition, CS diets reduced the C18:1 and C18:2 cis-9, trans-11 contents in subcutaneous fat (P<0.05). The muscle from animals fed CS tended to higher C16:0 and C18:0 contents (P<0.11), and decreased C18:1, C18:2 cis-9, trans-11 and C18:3 contents (P<0.05) compared with SB. The Δ9-desaturase index was greater in muscle from animals fed SB (P<0.01). At 42 days of age, meat from cattle fed SB had a greater lipid oxidation rate (P<0.05). Meat from animals fed SB diets had less lightness and redness indices than meat from animals fed CS diets after 14 days of age. In conclusion, the addition of ground cottonseed in the finishing diets did increase the saturated fatty acid content of the longissimus dorsi. However, animals fed cottonseed exhibited greater lightness and redness of beef. In this study, the addition of vitamin E did not affect qualitative characteristics of meat.


Assuntos
Ração Animal/análise , Bovinos/fisiologia , Suplementos Nutricionais , Ácidos Graxos/metabolismo , Carne/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Peso Corporal , Óleo de Sementes de Algodão , Dieta/veterinária , Ácidos Graxos/análise , Metabolismo dos Lipídeos , Masculino , Carne/normas , Sementes , Soja , Gordura Subcutânea/metabolismo , Vitamina E/administração & dosagem
14.
J Anim Sci ; 92(12): 5426-36, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25403202

RESUMO

Degree of unsaturation of fatty acids, which is influenced by lipid source and level of metabolism in the rumen, is a major determinant in how dietary lipids affect genes that regulate beef marbling. A total of 28 Red Norte bulls with an initial live weight of 361±32 kg (P>0.05) were used in a completely randomized experimental design to analyze the expression of genes that are involved in lipid metabolism in the longissimus dorsi (LD) when diets contained soybean grain or rumen-protected fat, with or without monensin. Treatments were arranged as a 2×2 factorial, with 4 treatments and 7 replicates per treatment. Half of the animals that received soybean or rumen-protected fat were supplemented with 230 mg head(-1) d(-1) of monensin. Gene expression was analyzed by reverse-transcription quantitative PCR (RT-qPCR). Expression of sterol regulatory element-binding protein-1c (SREBP-1c) in the LD muscle was not affected by lipid source or monensin (P>0.05). There was an interaction effect (P<0.05) between lipid source and monensin for peroxisome proliferator-activated receptor α (PPAR-α) and stearoyl-CoA desaturase (SCD) expression, where greater gene expression was found in animals fed soybean plus monensin and the lower gene expression was found in animals fed rumen-protected fat plus monensin. Expression of lipoprotein lipase (LPL) and fatty acid-binding protein 4 (FABP4) were greater (P<0.05) in the LD muscle of animals fed soybean. Monensin had no effect on LPL and FABP4 expression when soybean without monensin was fed, but when rumen-protected fat was fed, monensin increased LPL expression and decreased FABP4 expression (P<0.05). Linoleic and arachidonic acids had negative correlations (P<0.05) with the expression of PPAR-α, SCD, FABP4, and LPL genes. PPAR-α gene expression was not correlated with SREBP-1c but was positively correlated with SCD, FABP4, LPL, and glutathione peroxidase (GPX1) gene expression (P<0.001). Lipid sources and monensin interact and alter the expression of PPAR-α, SCD, acetyl CoA carboxylase α (ACACA), LPL, FABP4, and GPX1. These changes in gene expression were most associated with arachidonic and α-linolenic acids and the ability of lipid sources and monensin to increase these fatty acids in tissues.


Assuntos
Bovinos/genética , Gorduras na Dieta/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Metabolismo dos Lipídeos/genética , Monensin/farmacologia , Músculo Esquelético/fisiologia , Análise de Variância , Animais , Bovinos/metabolismo , Primers do DNA/genética , Gorduras na Dieta/metabolismo , Suplementos Nutricionais , Proteínas de Ligação a Ácido Graxo/metabolismo , Ácidos Graxos/metabolismo , Metabolismo dos Lipídeos/fisiologia , Masculino , PPAR alfa/metabolismo , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Rúmen/metabolismo , Soja/metabolismo , Estearoil-CoA Dessaturase/metabolismo , Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo
15.
Meat Sci ; 96(1): 597-605, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24018278

RESUMO

The objective of the present study was to evaluate the meat quality and fatty acid (FA) profile of the muscle and subcutaneous fat of young bulls fed ground soybean grain (SB) or rumen protected fat (RPF) with (230 mg head(-1) day(-1)) or without monensin. Forty animals with an initial weight of 359 kg were allotted in a 2×2 factorial arrangement of treatments. The use of monensin increased the arachidonic and α-linolenic acids in the longissimus dorsi (LD) muscle and subcutaneous fat, respectively (P<0.05). The meat from the animals receiving RPF had greater C18:1 content (P<0.01). The CLA and C18:2 contents were greater in the LD muscle of the animals fed SB (P<0.01). However, α-C18:3 was greater in the LD muscle of animals fed RPF (P<0.01). In the subcutaneous fat, SB reduced C12:0 and C14:0 contents (P<0.01) and increased C18:0 (P<0.05). The inclusion of RPF increased the C18:1 and CLA contents (P<0.01) in the subcutaneous fat. Soybean elevated PUFA contents and increased susceptibility of muscle and subcutaneous fat to lipid oxidation.


Assuntos
Ácidos Linoleicos Conjugados/análise , Metabolismo dos Lipídeos/fisiologia , Carne/análise , Monensin/farmacologia , Soja/química , Ácido alfa-Linolênico/análise , Ração Animal/análise , Animais , Composição Corporal , Bovinos , Cromatografia Gasosa , Cor , Ácidos Graxos/análise , Qualidade dos Alimentos , Masculino , Monensin/análise , Músculo Esquelético/química , Rúmen/química , Gordura Subcutânea/química , Paladar , Substâncias Reativas com Ácido Tiobarbitúrico/análise
16.
Braz. j. morphol. sci ; 30(1): 21-27, 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-699326

RESUMO

The mdx mice model is widely used for Duchenne muscular dystrophy (DMD) studies, which is present in a high percentage from newborns human males. Therefore, the aim of this study was to evaluate possible morphological changes from spleens in these mice and to compare with normal mice (Mus musculus) in contribution to DMD understanding and its consequences on immune system by affected individuals. The study was performed by light and scanning electron microscopy (SEM) techniques beyond immunohistochemistry. Was found microscopically an increased number of lymph nodes and decreased in red pulp region by mdx, beyond a larger VEGF-C (vascular endothelial growth factor C) expression stimulates lymphangiogenesis in red pulp region from spleen. These findings suggest a spleen adaptation in order to supply immunological demand due upper respiratory infection, which are common in individuals affected by Duchenne muscular dystrophy.


Assuntos
Animais , Camundongos , Distrofia Muscular de Duchenne/imunologia , Sistema Imunitário , Infecções Respiratórias/imunologia , Linfócitos , Fator A de Crescimento do Endotélio Vascular , Eutanásia Animal , Camundongos Endogâmicos mdx , Microscopia Eletrônica de Varredura
17.
Braz. j. morphol. sci ; 30(3): 186-190, 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-699347

RESUMO

The Duchenne Muscular Dystrophy (DMD) is a recessive genetic disease linked to chromosome X. Thisdisease is characterized by an absence or dysfunction in the expression of dystrophin. Experimental modelsmdxare widely used for the development of research addressing the DMD. The objective of this research is tocontribute to a detailed study of possible renal morphological changes resulting from DMD. We used five pairsof kidneys frommdxmice and five from normal mice, which were subjected to measurement, light microscopy,and scanning electron microscopy. The morphological findings of kidneys frommdxmice are within thepatterns described in animal studies with severe dehydration, which exhibit signs of diffuse hemorrhage inthe cortical and medullary area, while the glomeruli in the cortical region showed a decrease in urinary space,located between the Bowman’s capsule and the inner cell mass of the glomeruli. However, future experimentswith animals in different ages can assist in the proving of the morphological changes found here.


Assuntos
Animais , Masculino , Camundongos , Desidratação , Distrofia Muscular de Duchenne/complicações , Glomérulos Renais/anatomia & histologia , Rim/anatomia & histologia , Animais de Laboratório , Camundongos Endogâmicos mdx/anatomia & histologia , Eutanásia Animal , Microscopia Eletrônica de Varredura
18.
Int Endod J ; 45(12): 1080-90, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22747502

RESUMO

AIM: To compare cells from normal and inflamed human dental pulps regarding the presence of stem cells, their proliferation and differentiation potential. METHODOLOGY: Human dental pulp stem cells (hDPSCs) were isolated from normal (DPSC-N) and inflamed dental pulps (DPSC-I). They were compared in respect to proliferation (MTT assay), morphology and STRO-1 expression. STRO-1-positive cells were subject to proliferation (MTT and CFU counting) and morphological analyses and then submitted to odonto-osteogenic, adipogenic and condrogenic differentiation. Differentiated cells were evaluated concerning morphology and the expression, by qRT-PCR, of BSP, LPL and SOX-9 genes. The amount of mineralized matrix produced after odonto-osteogenic differentiation was compared with quantitative Alizarin Red staining. RESULTS: No difference was observed in the morphology and in the proliferation rate of DPSC-N and DPSC-I either before or after separation of STRO-1-positive cells. These cells represented 0.46% (±0.14) and 0.43% (±0.19) of the cell population from normal and inflamed dental pulps, respectively. Both DPSC-N and DPSC-I were capable of differentiating under the three assayed conditions and presented similar patterns for BSP, LPL and SOX-9 expression. Mineralized matrix production was also compatible. In all the quantitative experiments, differences were found between cells from each patient, either from normal or from inflamed pulps. Nonetheless, there was no statistical difference between these two groups. CONCLUSION: The morphology, proliferation rate and differentiation potential of DPSC-I were similar to the observed in DPSC-N, thus demonstrating that the inflammatory process did not affect the stem cell properties that were assessed.


Assuntos
Polpa Dentária/citologia , Células-Tronco Mesenquimais , Pulpite/patologia , Adipogenia , Adolescente , Adulto , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Condrogênese , Citometria de Fluxo , Humanos , Imunofenotipagem , Sialoproteína de Ligação à Integrina/biossíntese , Lipase Lipoproteica/biossíntese , Metaloproteinase 3 da Matriz/biossíntese , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/metabolismo , Células-Tronco Mesenquimais/fisiologia , Osteogênese , Regeneração , Fatores de Transcrição SOX9/biossíntese , Adulto Jovem
19.
Int J Oral Maxillofac Surg ; 41(12): 1566-70, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22727360

RESUMO

This study reports the case of a patient with a severely resorbed mandible who was treated without a bone graft, using short implants, internal rigid fixation, rhBMP-2 and ß-tricalcium phosphate. A 76-year-old woman, with a severely resorbed mandible (less than 3mm), reported a history of nearly 25 years of complete edentulism and consecutive treatment failures, with total bilateral exposed inferior alveolar nerves and complete bone resorption of the inferior border in some areas. The treatment of choice was the placement of a 2.0mm thick unilock bone plate (MatrixMandible, Synthes Maxillofacial, Paoli, PA, USA), to reinforce the mandible. Eight short implants with a regular platform (Nobel Biocare, Goteborg, Sweden) were placed: three on the external oblique line on both sides and two on the symphysis. In order to augment mandible height and coat the exposed thread of the anterior implants, rhBMP-2 (Infuse Bone, Meditronic Sofamor Danek, Memphis, TN, USA) and ß-tricalcium phosphate (Cerasorb; Curasan, Kleinostheim, Germany) were used. Four 1.3 mmL miniplates were placed to support the graft. 14 months after surgery, the patient was satisfied and had excellent function without complications.


Assuntos
Proteína Morfogenética Óssea 2/uso terapêutico , Doenças Mandibulares/tratamento farmacológico , Idoso , Proteína Morfogenética Óssea 2/administração & dosagem , Implantes Dentários , Feminino , Humanos , Doenças Mandibulares/patologia , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/uso terapêutico
20.
Cytotechnology ; 64(5): 511-21, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22328147

RESUMO

Isolation of mesenchymal stem cells (MSCs) from umbilical cord blood (UCB) from full-term deliveries is a laborious, time-consuming process that results in a low yield of cells. In this study we identified parameters that can be helpful for a successful isolation of UCB-MSCs. According to our findings, chances for a well succeeded isolation of these cells are higher when MSCs were isolated from UCB collected from normal full-term pregnancies that did not last over 37 weeks. Besides the duration of pregnancy, blood volume and storage period of the UCB should also be considered for a successful isolation of these cells. Here, we found that the ideal blood volume collected should be above 80 mL and the period of storage should not exceed 6 h. We characterized UCB-MSCs by morphologic, immunophenotypic, protein/gene expression and by adipogenic differentiation potential. Isolated UCB-MSCs showed fibroblast-like morphology and the capacity of differentiating into adipocyte-like cells. Looking for markers of the undifferentiated status of UCB-MSCs, we analyzed the UCB-MSCs' protein expression profile along different time periods of the differentiation process into adipocyte-like cells. Our results showed that there is a decrease in the expression of the markers CD73, CD90, and CD105 that correlates to the degree of differentiation of UCB-MSCs We suggest that CD90 can be used as a mark to follow the differentiation commitment degree of MSCs. Microarray results showed an up-regulation of genes related to the adipogenesis process and to redox metabolism in the adipocyte-like differentiated MSCs. Our study provides information on a group of parameters that may help with successful isolation and consequently with characterization of the differentiated/undifferentiated status of UCB-MSCs, which will be useful to monitor the differentiation commitment of UCB-MSC and further facilitate the application of those cells in stem-cell therapy.

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