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1.
J Thromb Haemost ; 16(10): 2035-2043, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-29993188

RESUMO

Essentials Missense mutations often impair protein folding, and thus intracellular trafficking and secretion. Cellular models of severe type I hemophilia B were challenged with chaperone-like compounds. Sodium phenylbutyrate improved intracellular trafficking and secretion of the frequent p.R294Q. The increased coagulant activity levels (∼3%) of p.R294Q would ameliorate the bleeding phenotype. SUMMARY: Background Missense mutations often impair protein folding and intracellular processing, which can be improved by small compounds with chaperone-like activity. However, little has been done in coagulopathies, where even modest increases of functional levels could have therapeutic implications. Objectives To rescue the expression of factor IX (FIX) variants affected by missense mutations associated with type I hemophilia B (HB) through chaperone-like compounds. Methods Expression studies of recombinant (r)FIX variants and evaluation of secreted levels (ELISA), intracellular trafficking (immunofluorescence) and activity (coagulant assays) before and after treatment of cells with chaperone-like compounds. Results As a model we chose the most frequent HB mutation (p.R294Q, ~100 patients), compared with other recurrent mutations associated with severe/moderate type I HB. Immunofluorescence studies revealed retention of rFIX variants in the endoplasmic reticulum and negligible localization in the Golgi, thus indicating impaired intracellular trafficking. Consistently, and in agreement with coagulation phenotypes in patients, all missense mutations resulted in impaired secretion (< 1% wild-type rFIX). Sodium phenylbutyrate (NaPBA) quantitatively improved trafficking to the Golgi and dose dependently promoted secretion (from 0.3 ± 0.1% to 1.5 ± 0.3%) only of the rFIX-294Q variant. Noticeably, this variant displayed a specific coagulant activity that was higher (~2.0 fold) than that of wild-type rFIX in all treatment conditions. Importantly, coagulant activity was concurrently increased to levels (3.0 ± 0.9%) that, if achieved in patients, would ameliorate the bleeding phenotype. Conclusions Altogether, our data detail molecular mechanisms underlying type I HB and candidate NaPBA as affordable 'personalized' therapeutics for patients affected by the highly frequent p.R294Q mutation, and with reduced access to substitutive therapy.


Assuntos
Coagulação Sanguínea/efeitos dos fármacos , Fator IX/genética , Fator IX/metabolismo , Hemofilia B/tratamento farmacológico , Mutação de Sentido Incorreto , Fenilbutiratos/farmacologia , Relação Dose-Resposta a Droga , Células HEK293 , Hemofilia B/sangue , Hemofilia B/genética , Humanos , Transporte Proteico , Via Secretória
2.
J Thromb Haemost ; 14(10): 1994-2000, 2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27513915

RESUMO

Essentials Potentially null homozygous Factor(F)7 nonsense mutations are associated to variable bleeding symptoms. Readthrough of p.Ser112X (life-threatening) and p.Cys132X (moderate) stop codons was investigated. Readthrough-mediated insertion of wild-type or tolerated residues produce functional proteins. Functional readthrough over homozygous F7 nonsense mutations contributes to the bleeding phenotype. SUMMARY: Background Whereas the rare homozygous nonsense mutations causing factor (F)VII deficiency may predict null conditions that are almost completely incompatible with life, they are associated with appreciable differences in hemorrhagic symptoms. The misrecognition of premature stop codons (readthrough) may account for variable levels of functional full-length proteins. Objectives To experimentally evaluate the basal and drug-induced levels of FVII resulting from the homozygous p.Cys132X and p.Ser112X nonsense mutations that are associated with moderate (132X) or life-threatening (112X) symptoms, and that are predicted to undergo readthrough with (132X) or without (112X) production of wild-type FVII. Methods We transiently expressed recombinant FVII (rFVII) nonsense and missense variants in human embryonic kidney 293 cells, and evaluated secreted FVII protein and functional levels by ELISA, activated FX generation, and coagulation assays. Results The levels of functional FVII produced by p.Cys132X and p.Ser112X mutants (rFVII-132X, 1.1% ± 0.2% of wild-type rFVII; rFVII-112X, 0.5% ± 0.1% of wild-type rFVII) were compatible with the occurrence of spontaneous readthrough, which was magnified by the addition of G418 - up to 12% of the wild-type value for the rFVII-132X nonsense variant. The predicted missense variants arising from readthrough abolished (rFVII-132Trp/Arg) or reduced (rFVII-112Trp/Cys/Arg, 22-45% of wild-type levels) secretion and function. These data suggest that the appreciable rescue of p.Cys132X function was driven by reinsertion of the wild-type residue, whereas the minimal p.Ser112X function was explained by missense changes permitting FVII secretion and function. Conclusions The extent of functional readthrough might explain differences in the bleeding phenotype of patients homozygous for F7 nonsense mutations, and prevent null conditions even for the most readthrough-unfavorable mutations.


Assuntos
Códon sem Sentido , Deficiência do Fator VII/genética , Fator VII/genética , Mutação , Coagulação Sanguínea , Códon de Terminação , Fator VII/metabolismo , Vetores Genéticos , Genótipo , Células HEK293 , Hemorragia , Homozigoto , Humanos , Mutagênese , Mutação de Sentido Incorreto , Fenótipo , Proteínas Recombinantes/metabolismo
3.
J Thromb Haemost ; 13(8): 1468-74, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26083275

RESUMO

BACKGROUND: The homologous coagulation factor X (FX), VII (FVII), IX (FIX) and protein C (PC) display striking differences in the carboxyl-terminus, with that of FX being the most extended. This region is essential for FVII, FIX and PC secretion. OBJECTIVES: To provide experimental evidence for the role of the FX carboxyl-terminus. METHODS: Recombinant FX (rFX) variants were expressed in multiple eukaryotic cell systems. Protein and activity levels were evaluated by ELISA, coagulant and amidolytic assays. RESULTS AND DISCUSSION: Expression of a panel of progressively truncated rFX variants in HEK293 cells revealed that the deletion of up to 21 residues in the carboxyl-terminus did not significantly affect secreted protein levels, as confirmed in HepG2 and BHK21 cells. In contrast, chimeric rFX-FVII variants with swapped terminal residues showed severely reduced levels. The truncated rFX variants revealed normal amidolytic activity, suggesting an intact active site. Intriguingly, these variants, which included that resembling the activated FXß form once cleaved, also displayed remarkable or normal pro-coagulant capacity in PT- and aPTT-based assays. This supports the hypothesis that subjects with nonsense mutations in the FX carboxyl-terminus, so far never identified, would be asymptomatic. CONCLUSIONS: For the first time we demonstrate that the FX carboxyl-terminal region downstream of residue K467 is not essential for secretion and provides a modest contribution to pro-coagulant properties. These findings, which might suggest an involvement of the carboxyl-terminal region in the divergence of the homologous FX, FVII, FIX and PC, help to interpret the mutational pattern of FX deficiency.


Assuntos
Coagulação Sanguínea , Fator X/metabolismo , Hepatócitos/metabolismo , Animais , Cricetinae , Fator X/química , Fator X/genética , Células HEK293 , Células Hep G2 , Humanos , Mutagênese Sítio-Dirigida , Mutação , Tempo de Tromboplastina Parcial , Estrutura Terciária de Proteína , Tempo de Protrombina , Relação Estrutura-Atividade , Transfecção
4.
Biochim Biophys Acta ; 1854(10 Pt A): 1351-6, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26012870

RESUMO

Alterations in coagulation factor X (FX) activation, mediated by the extrinsic VIIa/tissue factor (FVIIa/TF) or the intrinsic factor IXa/factor VIIIa (FIXa/FVIIIa) complexes, can result in hemorrhagic/prothrombotic tendencies. However, the molecular determinants involved in substrate recognition by these enzymes are poorly defined. Here, we investigated the role of arginine 386 (chymotrypsin numbering c202), a surface-exposed residue on the FX catalytic domain. The naturally occurring FX386Cys mutant and FX386Ala variant were characterized. Despite the unpaired cysteine, recombinant (r)FX386Cys was efficiently secreted (88.6±21.3% of rFXwt) and possessed normal clearance in mice. rFX386Cys was also normally activated by FVIIa/TF and displayed intact amidolytic activity. In contrast, rFX386Cys activation by the FIXa/FVIIIa complex was 4.5-fold reduced, which was driven by a decrease in the kcat (1.6∗10(-4) s(-1) vs 5.8∗10(-4) s(-1), rFXwt). The virtually unaltered Km (70.6 nM vs 55.6nM, rFXwt) suggested no major alterations in the FX substrate exosite. Functional assays in plasma supplemented with rFX386Cys indicated a remarkable reduction in the thrombin generation rate and thus in coagulation efficiency. Consistently, the rFX386Ala variant displayed similar biochemical features suggesting that global changes at position 386 impact the intrinsic pathway activation. These data indicate that the FXArg386 is involved in FIXa/FVIIIa-mediated FX activation and help in elucidating the bleeding tendency associated with the FX386Cys in a rare FX deficiency case. Taking advantage of the unpaired cysteine, the rFX386Cys mutant may be efficiently targeted by thiol-specific ligands and represent a valuable tool to study FX structure-function relationships both in vitro and in vivo.


Assuntos
Coagulação Sanguínea/genética , Fator X/metabolismo , Fator Xa/metabolismo , Mutação , Animais , Testes de Coagulação Sanguínea , Domínio Catalítico , Fator IXa/genética , Fator IXa/metabolismo , Fator VIIIa/genética , Fator VIIIa/metabolismo , Fator X/química , Fator X/genética , Fator Xa/química , Fator Xa/genética , Células HEK293 , Humanos , Cinética , Metaloendopeptidases/genética , Metaloendopeptidases/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Modelos Moleculares , Ligação Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Trombina/genética , Trombina/metabolismo
7.
J Thromb Haemost ; 12(2): 177-85, 2014 02.
Artigo em Inglês | MEDLINE | ID: mdl-24738135

RESUMO

BACKGROUND: The ability of the spliceosomal small nuclear RNA U1 (U1snRNA) to rescue pre-mRNA splicing impaired by mutations makes it an attractive therapeutic molecule. Coagulation factor deficiencies due to splicing mutations are relatively frequent and could therefore benefit from this strategy. However, the effects of U1snRNAs in vivo remain unknown. OBJECTIVES: To assess the rescue of the F7 c.859+5G>A splicing mutation (FVII+5A), causing severe human factor VII (hFVII) deficiency, by the modified U1snRNA+5a (U1+5a) in a murine model. METHODS: Mice expressing the human F7 c.859+5G>A mutant were generated following liver-directed expression by plasmid or recombinant adeno-associated viral (AAV) vector administration. The rescue of the splice-site defective pre-mRNA by U1+5a was monitored in liver and plasma through hFVII-specific assays. RESULTS: Injection of plasmids encoding the U1+5a rescued plasma hFVII levels, which increased from undetectable to ~8.5% of those obtained with the wild-type hFVII plasmid control. To assess long-term effects, mice were injected with low and high doses of two AAV vectors encoding the FVII+5A splice site mutant as template to be corrected by U1+5a. This strategy resulted in hFVII plasma levels of 3.9 ± 0.8 or 23.3 ± 5.1 ng mL⁻¹ in a dose-dependent manner, corresponding in patients to circulating FVII levels of ~1-4.5% of normal. Moreover, in both experimental models, we also detected correctly spliced hFVII transcripts and hFVII-positive cells in liver cells. CONCLUSIONS: Here we provide the first in vivo proof of-principle of the rescue of the expression of a splicing-defective F7 mutant by U1snRNAs, thus highlighting their therapeutic potential in coagulation disorders.


Assuntos
Transtornos da Coagulação Sanguínea/genética , Expressão Gênica/genética , Splicing de RNA , RNA Nuclear Pequeno/genética , Animais , Sequência de Bases , Primers do DNA , Vetores Genéticos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Reação em Cadeia da Polimerase Via Transcriptase Reversa
9.
Transplant Proc ; 44(7): 2033-5, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22974900

RESUMO

Transplantation in patients with congenital bleeding disorders is a challenge requiring an integrated approach of various specialists. Renal transplantation, the most frequent type of solid organ transplantation, is rarely performed in individuals with congenital hemorrhagic disorders. We performed a renal transplantation in a 53-year-old man with end-stage renal disease and congenital coagulation factor VII deficiency, a rare bleeding disorder with a peculiar clinical picture requiring replacement therapy in surgical interventions. Perioperative bleeding was successfully prevented by administration of recombinant activated factor VII. Treatment schedule, administration rate, and long-term follow-up are reported in detail. Our report confirmed the feasibility and safety of recombinant activated factor VII in major surgical procedures like solid organ transplantations. Success requires evaluation of doses and therapeutic schedules as well as a multidisciplinary approach.


Assuntos
Transplante de Rim , Estudos de Viabilidade , Humanos , Falência Renal Crônica/cirurgia , Masculino , Pessoa de Meia-Idade
10.
J Thromb Haemost ; 9(11): 2143-52, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21854538

RESUMO

Substitutive therapy has significantly ameliorated the quality of life of patients with coagulation factor deficiencies. However, there are some limitations that support research towards alternative therapeutic approaches. Here we focus on the rescue of coagulation factor biosynthesis by targeting the RNA processing and translation, which would permit restoration of the altered gene expression while maintaining the gene regulation in the physiological tissues. The essential prerequisite of the three reported RNA-based correction approaches (i-iii), which rely on mutation types and are applicable even to large size mRNAs, is the presence in cells of the precursor (pre-mRNA) or mature mRNA forms. (i) In the F7 gene, modification of the small nuclear RNA U1 (U1 snRNA), the key component of the spliceosomal U1 ribonucleoprotein, re-directs correct usage of a mutated exon-intron junction, triggering synthesis of correct mRNA and secretion of functional factor (F)VII. (ii) Spliceosome-mediated RNA trans-splicing (SMaRT) between mutated and engineered pre-mRNAs produces normal FVIII mRNA and secretion of functional protein. (iii) Aminoglycoside drugs induce ribosome readthrough and suppress premature translation termination caused by nonsense mutations in FVII, VIII and IX. The rescued expression levels ranged from very low (aminoglycosides) to moderate (U1 snRNA and SMaRT), which could result in amelioration of the disease phenotypes. These findings prompt further studies aimed at demonstrating the clinical translatability of RNA-based strategies, which might open new avenues in the treatment of coagulation factor deficiencies.


Assuntos
Transtornos de Proteínas de Coagulação/terapia , Proteínas Mutantes/antagonistas & inibidores , RNA/uso terapêutico , Fatores de Coagulação Sanguínea/genética , Terapia Genética , Humanos , Proteínas Mutantes/efeitos dos fármacos , Proteínas Mutantes/genética , Splicing de RNA
11.
Clin Biomech (Bristol, Avon) ; 26(9): 937-43, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21689874

RESUMO

BACKGROUND: The present study examines the hand movements of children with cerebral palsy during functional tests and compares the childrens' performance with and without the aid of an orthosis that provides wrist extension and thumb abduction. METHODS: The range of motion of the trapeziometacarpal joint was assessed for 32 participants via a reflexive markers image system. Observed motions included flexion-extension and abduction-adduction motions performed in the course of four tests for manual ability; the rest position, lateral and tripod pinches and cylindrical grasp. Muscle strength and manual ability were evaluated using dynamometry and the Jebsen-Taylor test. FINDINGS: The range of motion tests for the rest position, lateral and tripod pinches and cylindrical grasp demonstrated improvements from 17% to 42% (P<0.001) for flexion/extension and from 36% to 54% for abduction/adduction (P<0.001) with the use of the orthosis. Dynamometry measurements showed that the improvement in muscle strength obtained through use of the orthosis was 50% (P<0.001). Improvements in the time required to perform the movements as determined using the Jebsen-Taylor test varied from 13% to 24% (P<0.01) for the four considered tests of manual ability. INTERPRETATION: The orthosis improved the range of motion of the trapeziometacarpal joint, muscle strength and manual ability. The combination of the three techniques may provide the basis for a quantitative assessment of hand dysfunction/improvement in cerebral palsy that will ultimately guide health professionals in their clinical interventions.


Assuntos
Paralisia Cerebral/fisiopatologia , Mãos/anatomia & histologia , Punho/anatomia & histologia , Criança , Pré-Escolar , Desenho de Equipamento , Feminino , Mãos/fisiologia , Força da Mão , Humanos , Estudos Longitudinais , Masculino , Movimento , Músculos/fisiologia , Fenômenos Fisiológicos Musculoesqueléticos , Aparelhos Ortopédicos , Amplitude de Movimento Articular , Reprodutibilidade dos Testes , Polegar/anatomia & histologia , Punho/fisiologia , Articulação do Punho/fisiologia
12.
Comput Methods Biomech Biomed Engin ; 14(4): 365-70, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21442494

RESUMO

The present study was conducted in order to establish a methodology based on the finite element method to simulate the contraction of the pelvic floor (PF) muscles. In the generated finite element model, a downward pressure of 90 cm H(2)O was applied, while actively contracting the PF muscles with different degrees of muscular activation (10, 50 and 100%). The finite element methodology of the active contraction behaviour proposed in this study is adequate to simulate PF muscle contraction with different degrees of muscular activation. In this case, in particular, for an activation of 100%, the numerical model was able to displace the pubovisceral muscle in a range of values very similar to the displacement found in the magnetic resonance imaging data. In the analysed case study, it would be possible to conclude that an intensity contraction of 50% would be necessary to produce enough stiffness to avoid possible urine loss.


Assuntos
Contração Muscular , Diafragma da Pelve/fisiopatologia , Adulto , Feminino , Análise de Elementos Finitos , Humanos , Imageamento por Ressonância Magnética , Modelos Biológicos , Diafragma da Pelve/anatomia & histologia , Incontinência Urinária por Estresse/fisiopatologia
13.
J Thromb Haemost ; 7(5): 774-9, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19245420

RESUMO

SUMMARY BACKGROUND: The autosomally-inherited factor VII (FVII) deficiency and X-linked hemophilia B offer an attractive model to investigate whether reduced levels of FVII and FIX, acting in the initiation and amplification of coagulation respectively, influence hemostasis to a different extent in relation to age and bleeding site. METHODS: Hemophilia B patients (n = 296) and FVII-deficient males (n = 109) were compared for FVII/FIX clotting activity, F7/F9 genotypes and clinical phenotypes in a retrospective, multi-centre, cohort study. RESULTS: Major clinical differences between diseases were observed. Bleeding occurred earlier in hemophilia B (median age 2.0 years, IR 0.9-5.0) than in FVII deficiency (5.2 years, IR 1.9-15.5) and the bleeding-free survival in FVII deficiency was similar to that observed in 'mild' hemophilia B (P = 0.96). The most frequent disease-presenting symptoms in hemophilia B (hematomas and oral bleeding) differed from those in FVII deficiency (epistaxis and central nervous system bleeding). Differences were confirmed by analysis of FVII-deficient women. CONCLUSIONS: Our data support the notion that low FVII levels sustain hemostasis better than similarly reduced FIX levels. On the other hand, minute amounts of FVII, differently to FIX, are needed to prevent fatal bleeding, as indicated by the rarity of null mutations and the associated life-threatening symptoms in FVII deficiency, which contributes towards shaping clinical differences between diseases in the lowest factor level range. Differences between diseases are only partially explained by mutational patterns and could pertain to the specific roles of FVII and FIX in coagulation phases and to vascular bed-specific components.


Assuntos
Deficiência do Fator VII/fisiopatologia , Hemofilia B/fisiopatologia , Hemorragia/fisiopatologia , Intervalo Livre de Doença , Humanos , Inquéritos e Questionários
14.
Photomed Laser Surg ; 26(4): 315-21, 2008 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-18637717

RESUMO

OBJECTIVE: The aims of the present study were to investigate the effect of low-intensity laser irradiation on the total number of mast cells as well as the percentage of degranulation in human gingiva. Blood vessel dilation was also evaluated. BACKGROUND DATA: It has been proposed that low-intensity laser irradiation can ameliorate pain, swelling, and inflammation. In periodontal tissue, mast cells may influence either the destructive events or the defense mechanism against periodontal disease via secretion of cytokines and through cellular migration to improve the healing process. Mast cells play an important role in the inflammatory process. METHODS: Twenty patients with gingival enlargement indicated for gingivectomy were selected. Gingival fragments were obtained from each patient and divided into three different groups before surgery. One fragment was removed without any irradiation. The two others were submitted to punctual irradiation with an energy density of 8 J/cm(2) at an output power of 50 mW at 36 Hz for 36 sec before gingivectomy. Nondegranulated and degranulated mast cells were counted in five areas of the gingival fragment connective tissue. Major and minor diameters of the blood vessels were also measured. RESULTS: Both red and infrared radiation promoted a significant increase in mast cell degranulation compared to controls; however, no statistically significant differences (p > 0.05) were observed between the irradiated groups. No significant differences among the groups were observed regarding blood vessel size. CONCLUSION: The results suggests that red and infrared wavelengths promote mast cell degranulation in human gingival tissue, although no dilation of blood vessels was observed. The effects of premature degranulation of mast cells in human tissue and the laser radiation protocol applied in this study encourage further investigations to extend these results into clinical practice.


Assuntos
Degranulação Celular/efeitos dos fármacos , Gengiva/efeitos da radiação , Crescimento Excessivo da Gengiva/radioterapia , Terapia com Luz de Baixa Intensidade , Mastócitos/efeitos da radiação , Adulto , Feminino , Gengiva/patologia , Gengiva/cirurgia , Crescimento Excessivo da Gengiva/cirurgia , Gengivectomia , Humanos , Masculino , Pessoa de Meia-Idade , Vasodilatação/efeitos dos fármacos
16.
Pediatr Med Chir ; 30(1): 48-50, 2008.
Artigo em Italiano | MEDLINE | ID: mdl-18491680

RESUMO

Hepatitis A virus infection is usually asymptomatic in children. Classic symptomatic forms and atypical clinical manifestations are known. We report a paediatric case of hepatitis A with marked cholestasis, treated with steroids, and with an unusual prolonged course.


Assuntos
Hepatite A , Adolescente , Anti-Inflamatórios/administração & dosagem , Anti-Inflamatórios/uso terapêutico , Colestase/etiologia , Seguimentos , Hepatite A/complicações , Hepatite A/diagnóstico , Hepatite A/tratamento farmacológico , Humanos , Masculino , Prednisona/administração & dosagem , Prednisona/uso terapêutico , Fatores de Tempo , Resultado do Tratamento
17.
J Thromb Haemost ; 6(5): 797-803, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18315553

RESUMO

BACKGROUND: Combined vitamin K-dependent clotting factor (VKCF) deficiency type 2 (VKCFD2) is a rare bleeding disorder caused by mutated vitamin K 2,3-epoxide reductase complex subunit 1 (VKORC1) gene. METHODS AND RESULTS: An Italian patient with moderate to severe bleeding tendency was genotyped, and found to be homozygous for the unique VKORC1 mutation (Arg98Trp) so far detected in VKCFD2. The activity levels of VKCFs were differentially reduced, and inversely related to the previously estimated affinity of procoagulant factor propeptides for the gamma-carboxylase. The normal (factor IX) or reduced antigen levels (other VKCFs) produced a gradient in specific activities. Vitamin K supplementations resulted in reproducible, fast and sustained normalization of PT and APTT. At 24 h the activity/antigen ratios of VKCFs were close to normal, and activity levels were completely (factor VII and IX), virtually (prothrombin, factor X and protein C) or partially (protein S) restored. Thrombin generation assays showed a markedly shortened lag time. The time to peak observed at low tissue factor concentration, potentially mimicking the physiological trigger and able to highlight the effect of reduced protein S levels, was shorter than that in pooled normal plasma. At 72 h the thrombin generation times were normal, and the decrease in activity of procoagulant VKCFs was inversely related to their half-life in plasma. The improved coagulation phenotype permitted the uneventful clinical course after invasive diagnostic procedures. CONCLUSIONS: Modification of coagulation phenotypes in VKCFD2 after vitamin K supplementation was clinically beneficial, and provided valuable patterns of factor specific biosynthesis, half-life and decay.


Assuntos
Transtornos da Coagulação Sanguínea/genética , Oxigenases de Função Mista/genética , Vitamina K/uso terapêutico , Adulto , Transtornos da Coagulação Sanguínea/etiologia , Testes de Coagulação Sanguínea , Feminino , Meia-Vida , Homozigoto , Humanos , Oxigenases de Função Mista/deficiência , Mutação , Resultado do Tratamento , Vitamina K Epóxido Redutases
18.
Arq. bras. med. vet. zootec ; 58(6): 1123-1131, dez. 2006. tab
Artigo em Português | LILACS | ID: lil-455058

RESUMO

Descreve-se uma nova metodologia para medir o temperamento dos bovinos por meio da reatividade, obtida por um dispositivo eletrônico que quantifica o número e a intensidade dos movimentos do animal em ambiente de contenção móvel, numa escala de 1 a 9.999 pontos. A reatividade foi comparada a características determinadas por outros dois métodos tradicionais para quantificar o temperamento bovino: a velocidade de fuga e o escore de temperamento. Foram utilizados 610 bovinos da raça Nelore, com média de idade de 467 dias e provenientes de dois rebanhos. As observações da reatividade não se ajustaram às distribuições normal, Poisson e binomial negativa. A padronização e as transformações logarítmicas neperianas e na base 10 não foram eficientes para adequar os dados à distribuição normal. Os animais avaliados para a característica apresentaram grande variabilidade fenotípica, com reatividade média de 534±447 pontos na escala original, mediana 442 pontos, moda 372 pontos, variação de 1 a 2.218 pontos e coeficiente de variação de 84 por cento. A correlação de classificação de resíduos encontrada entre reatividade e escore de temperamento variou de 0,75 a 0,85 (P<0,001) e foi superior em valores absolutos às correlações entre reatividade e velocidade de fuga e entre velocidade de fuga e escore de temperamento. A nova metodologia é eficiente para quantificar o temperamento de animais da raça Nelore e possui vantagens em relação às características velocidade de fuga e escore de temperamento. A obtenção da reatividade animal é rápida, prática, objetiva e segura para quem a faz, além de apresentar grande variabilidade fenotípica.


This paper describes a new methodology to access bovine temperament using it's reactivity eletronically measured as the intensity and count of movements in a mobile cage, using a scale from 1 to 9,999 points. The new trait, reactivity, was compared to two other traditional methods, flying speed and temperament scores. Six hundred and ten contemporary Nellores, with average age of 467 days and from two herds were used. Reactivity data did not fit Normal nor Poisson nor Negative Binomial Distibutions. Standartized reactivity data, neperian and base 10 log reactivity transformations were not successful in adjusting data to normal distribution. Reactivity presented large phenotipic variation, with average in the original scale of 534±447 points, median of 442, mode 372, range from 1 to 2,218 points and variation coefficient of 84 percent. Residual rank correlation between reactivity and temperament score vary from .75 to .85 (P<.001), and were larger than the ones between reactivity and flight speed and between flight speed and temperament score. The new methodology is efficient in measuring temperament of Nellore cattle and it has advantages in relation to the traits flight speed and temperament score. The measure of reactivity is easy, fast to get and safe to record. In addition, this trait presents large variability.


Assuntos
Animais , Bovinos , Comportamento Animal/fisiologia , Equipamentos de Medição de Riscos , Reatividade-Estabilidade , Temperamento/classificação
19.
Arq. bras. med. vet. zootec ; 58(6): 1123-1131, dez. 2006. tab, graf
Artigo em Português | VETINDEX | ID: vti-7326

RESUMO

Descreve-se uma nova metodologia para medir o temperamento dos bovinos por meio da reatividade, obtida por um dispositivo eletrônico que quantifica o número e a intensidade dos movimentos do animal em ambiente de contenção móvel, numa escala de 1 a 9.999 pontos. A reatividade foi comparada a características determinadas por outros dois métodos tradicionais para quantificar o temperamento bovino: a velocidade de fuga e o escore de temperamento. Foram utilizados 610 bovinos da raça Nelore, com média de idade de 467 dias e provenientes de dois rebanhos. As observações da reatividade não se ajustaram às distribuições normal, Poisson e binomial negativa. A padronização e as transformações logarítmicas neperianas e na base 10 não foram eficientes para adequar os dados à distribuição normal. Os animais avaliados para a característica apresentaram grande variabilidade fenotípica, com reatividade média de 534±447 pontos na escala original, mediana 442 pontos, moda 372 pontos, variação de 1 a 2.218 pontos e coeficiente de variação de 84%. A correlação de classificação de resíduos encontrada entre reatividade e escore de temperamento variou de 0,75 a 0,85 (P<0,001) e foi superior em valores absolutos às correlações entre reatividade e velocidade de fuga e entre velocidade de fuga e escore de temperamento. A nova metodologia é eficiente para quantificar o temperamento de animais da raça Nelore e possui vantagens em relação às características velocidade de fuga e escore de temperamento. A obtenção da reatividade animal é rápida, prática, objetiva e segura para quem a faz, além de apresentar grande variabilidade fenotípica.(AU)


This paper describes a new methodology to access bovine temperament using it's reactivity eletronically measured as the intensity and count of movements in a mobile cage, using a scale from 1 to 9,999 points. The new trait, reactivity, was compared to two other traditional methods, flying speed and temperament scores. Six hundred and ten contemporary Nellores, with average age of 467 days and from two herds were used. Reactivity data did not fit Normal nor Poisson nor Negative Binomial Distibutions. Standartized reactivity data, neperian and base 10 log reactivity transformations were not successful in adjusting data to normal distribution. Reactivity presented large phenotipic variation, with average in the original scale of 534±447 points, median of 442, mode 372, range from 1 to 2,218 points and variation coefficient of 84%. Residual rank correlation between reactivity and temperament score vary from .75 to .85 (P<.001), and were larger than the ones between reactivity and flight speed and between flight speed and temperament score. The new methodology is efficient in measuring temperament of Nellore cattle and it has advantages in relation to the traits flight speed and temperament score. The measure of reactivity is easy, fast to get and safe to record. In addition, this trait presents large variability.(AU)


Assuntos
Animais , Comportamento Animal/fisiologia , Reatividade-Estabilidade , Temperamento/classificação , Equipamentos de Medição de Riscos , Bovinos
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